Restriction map for pF1KB8466
##################################
# NO CUT ENZYMES (  132 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvrII       BamHI       BbrPI       BciVI       BclI        
BfaI        BfrI        BglII       BlnI        BpmI        
BsaI        BseAI       BsgI        BsiWI       BsmAI       
BsmBI       BsmFI       Bsp1407I    BspDI       BspEI       
BspLU11I    BsrDI       BsrGI       BssHII      Bst1107I    
BstBI       BstEII      BstPI       BstYI       BstZ17I     
Bsu36I      ClaI        CpoI        DraI        DraIII      
DrdI        Eam1105I    Eco47III    Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FbaI        FseI        HgaI        HindIII     HinfI       
HpaI        KpnI        MaeI        MfeI        MflI        
MluI        MroI        MseI        MunI        NcoI        
NdeI        NheI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PstI        PvuI        RsrII       
SalI        SbfI        ScaI        SfcI        SfiI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TaqI        TfiI        
Tru9I       Tsp509I     Tth111I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AcyI          710  GGCGCC               G[GA]^CG[CT]C
AfaI          436  GTAC                 GT^AC
AflIII         89  ACGTGT               A^C[GA][CT]GT
AlwI          138  GATCC                GGATC(4/5)
AlwNI          38  CAGAACCTG            CAG[ACGT][ACGT][ACGT]^CTG
BalI         1175  TGGCCA               TGG^CCA
BbeI          710  GGCGCC               GGCGC^C
BbvCI         509  CCTCAGC              CCTCAGC(-5/-2)
BcgI          858  CGAGGAGCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI          701  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      701  GCTCAGC              GC^T[ACGT]AGC
BsaAI         820  TACGTG               [CT]AC^GT[GA]
BsaBI        1236  GATGCGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         710  GGCGCC               G[GA]^CG[CT]C
BsaWI         471  TCCGGT               [AT]^CCGG[AT]
BsmI           48  GAATGC               GAATGC(1/-1)
BspHI         407  TCATGA               T^CATGA
BssSI         729  CTCGTG               CACGAG(-5/-1)
BstAPI        389  GCATATTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI        1166  CCAGCAGAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         701  GCTCAGC              GC^T[ACGT]AGC
Hin1I         710  GGCGCC               G[GA]^CG[CT]C
HincII        835  GTCAAC               GT[CT]^[GA]AC
HindII        835  GTCAAC               GT[CT]^[GA]AC
KasI          710  GGCGCC               G^GCGCC
KspI          555  CCGCGG               CCGC^GG
MamI         1236  GATGCGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        1175  TGGCCA               TGG^CCA
MscI         1175  TGGCCA               TGG^CCA
NarI          710  GGCGCC               GG^CGCC
NotI         1158  GCGGCCGC             GC^GGCCGC
PpuMI         977  GGGTCCT              [GA]G^G[AT]CC[CT]
RcaI          407  TCATGA               T^CATGA
RsaI          436  GTAC                 GT^AC
SacI          271  GAGCTC               GAGCT^C
SacII         555  CCGCGG               CCGC^GG
SexAI         152  ACCTGGT              A^CC[AT]GGT
SfoI          710  GGCGCC               GGC^GCC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AccI          148  GTCTAC               GT^[AC][GT]AC
              241  GTCTAC              
ApaI           99  GGGCCC               GGGCC^C
             1027  GGGCCC              
AviII        1004  TGCGCA               TGC^GCA
             1238  TGCGCA              
BbsI         1038  GAAGAC               GAAGAC(2/6)
              538  GTCTTC              
BglI           52  GCCTCCTCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              781  GCCGTGGTGGC         
BpuAI        1038  GAAGAC               GAAGAC(2/6)
              538  GTCTTC              
BspMI         568  ACCTGC               ACCTGC(4/8)
              677  ACCTGC              
BsrBI         447  CCGCTC               CCGCTC(-3/-3)
             1156  GAGCGG              
BsrFI         591  GCCGGC               [GA]^CCGG[CT]
              610  GCCGGC              
Cfr10I        591  GCCGGC               [GA]^CCGG[CT]
              610  GCCGGC              
DpnII         138  GATC                 ^GATC
             1128  GATC                
EarI          274  CTCTTC               CTCTTC(1/4)
              793  CTCTTC              
FspI         1004  TGCGCA               TGC^GCA
             1238  TGCGCA              
Ksp632I       274  CTCTTC               CTCTTC(1/4)
              793  CTCTTC              
MboI          138  GATC                 ^GATC
             1128  GATC                
NaeI          591  GCCGGC               GCC^GGC
              610  GCCGGC              
NdeII         138  GATC                 ^GATC
             1128  GATC                
NgoMIV        591  GCCGGC               G^CCGGC
              610  GCCGGC              
NspI            5  ACATGC               [GA]CATG^[CT]
              575  ACATGC              
PvuII         704  CAGCTG               CAG^CTG
              915  CAGCTG              
SapI          273  GCTCTTC              GCTCTTC(1/4)
              792  GCTCTTC             
Sau3AI        138  GATC                 ^GATC
             1128  GATC                
Tsp45I        232  GTCAC                ^GT[GC]AC
              763  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   65 enzymes) #
#########################################

AccII       AciI        AluI        AspHI       AvaI        
AvaII       BanI        BanII       BbvI        BcnI        
BmyI        BsaJI       BseRI       BsiEI       BsiHKAI     
BsiYI       BslI        BsoBI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       BstUI       Cac8I       
CfoI        Cfr13I      DdeI        DraII       DsaI        
EaeI        EagI        EclXI       Eco52I      EcoO109I    
EcoRII      FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HhaI        HinP1I      HpaII       
HphI        ItaI        MaeII       MaeIII      MboII       
MnlI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NlaIII      NlaIV       
Sau96I      ScrFI       SfaNI       TseI        TspRI       
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