Restriction map for pF1KB8466
 (Linear) (Six-base) MAPSORT of: KIBB8466.seq  Check: 1175  from: 1  to: 1254



KIBB8466.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:06  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     577    1254 
   Size:        577     677
 
AccI GT'mk_AC

Cuts at:      0     149     242    1254 
   Size:        149      93    1012

  Fragments arranged by size: 

                1012     149      93
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     212    1254 
   Size:        212    1042
 
AflIII A'CryG_T

Cuts at:      0      89    1254 
   Size:         89    1165
 
AleI CACnn'nnGTG

Cuts at:      0     729    1254 
   Size:        729     525
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     747     997    1254 
   Size:        747     250     257

  Fragments arranged by size: 

                 747     257     250
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     301     333    1254 
   Size:        301      32     921

  Fragments arranged by size: 

                 921     301      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      43    1254 
   Size:         43    1211
 
ApaI G_GGCC'C

Cuts at:      0     103    1031    1254 
   Size:        103     928     223

  Fragments arranged by size: 

                 928     223     103
 
AvaI C'yCGr_G

Cuts at:      0     543     586     856    1254 
   Size:        543      43     270     398

  Fragments arranged by size: 

                 543     398     270      43
 
BanI G'GyrC_C

Cuts at:      0     710     723    1141    1254 
   Size:        710      13     418     113

  Fragments arranged by size: 

                 710     418     113      13
 
BanII G_rGCy'C

Cuts at:      0     103     275     881    1019    1031    1254 
   Size:        103     172     606     138      12     223

  Fragments arranged by size: 

                 606     223     172     138     103      12
 
BbeI G_GCGC'C

Cuts at:      0     714    1254 
   Size:        714     540
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     531    1045    1254 
   Size:        531     514     209

  Fragments arranged by size: 

                 531     514     209
 
BbvCI CC'TCA_GC

Cuts at:      0     510    1254 
   Size:        510     744
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     847     881    1254 
   Size:        847      34     373

  Fragments arranged by size: 

                 847     373      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0      58     787    1254 
   Size:         58     729     467

  Fragments arranged by size: 

                 729     467      58
 
BlpI GC'TnA_GC

Cuts at:      0     702    1254 
   Size:        702     552
 
Bme1580I G_kGCm'C

Cuts at:      0     103     554     726     850    1031    1254 
   Size:        103     451     172     124     181     223

  Fragments arranged by size: 

                 451     223     181     172     124     103
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     646    1226    1254 
   Size:        646     580      28

  Fragments arranged by size: 

                 646     580      28
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      69    1254 
   Size:         69    1185
 
Bpu10I CC'TnA_GC

Cuts at:      0     510    1254 
   Size:        510     744
 
BsaAI yAC'GTr

Cuts at:      0     822    1254 
   Size:        822     432
 
BsaBI GATnn'nnATC

Cuts at:      0    1240    1254 
   Size:       1240      14
 
BsaHI Gr'CG_yC

Cuts at:      0     711    1254 
   Size:        711     543
 
BsaWI w'CCGG_w

Cuts at:      0     471    1254 
   Size:        471     783
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      45     874    1060    1063    1072    1075    1078    1090 
   Size:         45     829     186       3       9       3       3      12

Cuts at:   1090    1254 
   Size:        164

  Fragments arranged by size: 

                 829     186     164      45      12       9       3       3
                   3
 
BseYI C'CCAG_C

Cuts at:      0     177     282     706    1165    1254 
   Size:        177     105     424     459      89

  Fragments arranged by size: 

                 459     424     177     105      89
 
BsiEI CG_ry'CG

Cuts at:      0     632    1116    1162    1254 
   Size:        632     484      46      92

  Fragments arranged by size: 

                 632     484      92      46
 
BsiHKAI G_wGCw'C

Cuts at:      0     275     568     803     827    1254 
   Size:        275     293     235      24     427

  Fragments arranged by size: 

                 427     293     275     235      24
 
BsmI GAATG_Cn'

Cuts at:      0      54    1254 
   Size:         54    1200
 
Bsp1286I G_dGCh'C

Cuts at:      0     103     275     554     568     726     803     827     850 
   Size:        103     172     279      14     158      77      24      23

Cuts at:    850     881    1019    1031    1254 
   Size:         31     138      12     223

  Fragments arranged by size: 

                 279     223     172     158     138     103      77      31
                  24      23      14      12
 
BspHI T'CATG_A

Cuts at:      0     407    1254 
   Size:        407     847
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     577     686    1254 
   Size:        577     109     568

  Fragments arranged by size: 

                 577     568     109
 
BsrBI CCG'CTC

Cuts at:      0     449    1158    1254 
   Size:        449     709      96

  Fragments arranged by size: 

                 709     449      96
 
BsrFI r'CCGG_y

Cuts at:      0     591     610    1254 
   Size:        591      19     644

  Fragments arranged by size: 

                 644     591      19
 
BssSI C'ACGA_G

Cuts at:      0     729    1254 
   Size:        729     525
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     395    1254 
   Size:        395     859
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1173    1254 
   Size:       1173      81
 
BtgI C'CryG_G

Cuts at:      0     431     515     530     555     782    1254 
   Size:        431      84      15      25     227     472

  Fragments arranged by size: 

                 472     431     227      84      25      15
 
BtsI GCAGTG_nn'

Cuts at:      0     586     850    1254 
   Size:        586     264     404

  Fragments arranged by size: 

                 586     404     264
 
EaeI y'GGCC_r

Cuts at:      0     119     218     263     299     428     629     779    1113 
   Size:        119      99      45      36     129     201     150     334

Cuts at:   1113    1159    1175    1254 
   Size:         46      16      79

  Fragments arranged by size: 

                 334     201     150     129     119      99      79      46
                  45      36      16
 
EagI C'GGCC_G

Cuts at:      0     629    1113    1159    1254 
   Size:        629     484      46      95

  Fragments arranged by size: 

                 629     484      95      46
 
EarI CTCTTCn'nnn_

Cuts at:      0     280     799    1254 
   Size:        280     519     455

  Fragments arranged by size: 

                 519     455     280
 
EcoICRI GAG'CTC

Cuts at:      0     273    1254 
   Size:        273     981
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     212    1254 
   Size:        212    1042
 
EcoO109I rG'GnC_Cy

Cuts at:      0     978    1027    1028    1254 
   Size:        978      49       1     226

  Fragments arranged by size: 

                 978     226      49       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     984    1254 
   Size:        984     270
 
FspI TGC'GCA

Cuts at:      0    1006    1240    1254 
   Size:       1006     234      14

  Fragments arranged by size: 

                1006     234      14
 
FspAI rTGC'GCAy

Cuts at:      0    1240    1254 
   Size:       1240      14
 
HaeII r_GCGC'y

Cuts at:      0     262     714     750     793     901    1254 
   Size:        262     452      36      43     108     353

  Fragments arranged by size: 

                 452     353     262     108      43      36
 
HincII GTy'rAC

Cuts at:      0     837    1254 
   Size:        837     417
 
KasI G'GCGC_C

Cuts at:      0     710    1254 
   Size:        710     544
 
MscI TGG'CCA

Cuts at:      0    1177    1254 
   Size:       1177      77
 
MslI CAynn'nnrTG

Cuts at:      0     199     361     412     688     729     769     775    1254 
   Size:        199     162      51     276      41      40       6     479

  Fragments arranged by size: 

                 479     276     199     162      51      41      40       6
            
 
MspA1I CmG'CkG

Cuts at:      0     458     557     654     706     738     917    1148    1254 
   Size:        458      99      97      52      32     179     231     106

  Fragments arranged by size: 

                 458     231     179     106      99      97      52      32
            
 
NaeI GCC'GGC

Cuts at:      0     593     612    1254 
   Size:        593      19     642

  Fragments arranged by size: 

                 642     593      19
 
NarI GG'CG_CC

Cuts at:      0     711    1254 
   Size:        711     543
 
NgoMIV G'CCGG_C

Cuts at:      0     591     610    1254 
   Size:        591      19     644

  Fragments arranged by size: 

                 644     591      19
 
NotI GC'GGCC_GC

Cuts at:      0    1159    1254 
   Size:       1159      95
 
NspI r_CATG'y

Cuts at:      0       9     579    1254 
   Size:          9     570     675

  Fragments arranged by size: 

                 675     570       9
 
PasI CC'CwG_GG

Cuts at:      0      81    1254 
   Size:         81    1173
 
PpuMI rG'GwC_Cy

Cuts at:      0     978    1254 
   Size:        978     276
 
PspOMI G'GGCC_C

Cuts at:      0      99    1027    1254 
   Size:         99     928     227

  Fragments arranged by size: 

                 928     227      99
 
PvuII CAG'CTG

Cuts at:      0     706     917    1254 
   Size:        706     211     337

  Fragments arranged by size: 

                 706     337     211
 
SacI G_AGCT'C

Cuts at:      0     275    1254 
   Size:        275     979
 
SacII CC_GC'GG

Cuts at:      0     558    1254 
   Size:        558     696
 
SapI GCTCTTCn'nnn_

Cuts at:      0     280     799    1254 
   Size:        280     519     455

  Fragments arranged by size: 

                 519     455     280
 
SexAI A'CCwGG_T

Cuts at:      0     152    1254 
   Size:        152    1102
 
SfoI GGC'GCC

Cuts at:      0     712    1254 
   Size:        712     542
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     492    1254 
   Size:        492     762
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII     AleI     AlfI     AloI    AlwNI
     ApaI     AvaI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI
     BglI     BlpI Bme1580I     BmrI     BplI   Bpu10I    BsaAI    BsaBI
    BsaHI    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspHI    BspMI    BsrBI    BsrFI    BssSI   BstAPI    BstXI     BtgI
     BtsI     EaeI     EagI     EarI  EcoICRI  Eco57MI EcoO109I     FalI
     FspI    FspAI    HaeII   HincII     KasI     MscI     MslI   MspA1I
     NaeI     NarI   NgoMIV     NotI     NspI     PasI    PpuMI   PspOMI
    PvuII     SacI    SacII     SapI    SexAI     SfoI    TaqII

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BciVI
     BclI    BfrBI    BglII    BmgBI     BmtI     BpmI    BpuEI     BsaI
    BsaXI     BsgI    BsiWI    BsmBI    BspEI    BsrDI    BsrGI   BssHII
    BstBI   BstEII    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EciI    EcoNI    EcoRI    EcoRV     FseI
    Hin4I  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     NcoI
     NdeI     NheI     NruI     NsiI     PacI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PstI
     PvuI    RsrII     SalI    SanDI     SbfI     ScaI     SfcI     SfiI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI
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