Restriction map for pF1KB8461
##################################
# NO CUT ENZYMES (  163 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwI        AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BamHI       BanII       BbeI        BbrPI       
BcgI        BciVI       BclI        BfaI        BfrI        
BglII       BlnI        BsaAI       BsaBI       BsaI        
BsaWI       BseAI       BsgI        BsiWI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrDI       BsrFI       BsrGI       BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstYI       
BstZ17I     Bsu36I      Cfr10I      ClaI        CpoI        
DpnII       DraI        DraIII      DrdI        Eam1105I    
EarI        Eco47III    Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HaeII       HgaI        HincII      HindII      
HindIII     HpaI        KasI        KpnI        Ksp632I     
KspI        MaeI        MaeIII      MamI        MboI        
MfeI        MflI        MluI        MroI        MseI        
MspA1I      MunI        NaeI        NarI        NcoI        
NdeI        NdeII       NgoMIV      NheI        NlaIII      
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        Sau3AI      
SbfI        ScaI        SexAI       SfaNI       SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        TfiI        Tru9I       Tsp45I      Tsp509I     
Tth111I     Van91I      XbaI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AatII         226  GACGTC               GACGT^C
AcyI          226  GACGTC               G[GA]^CG[CT]C
AfaI           93  GTAC                 GT^AC
AlwNI         421  CAGGGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AspHI         468  GTGCTC               G[AT]GC[AT]^C
AvaII         351  GGACC                G^G[AT]CC
BalI          434  TGGCCA               TGG^CCA
BbsI          229  GTCTTC               GAAGAC(2/6)
BbvCI         535  CCTCAGC              CCTCAGC(-5/-2)
BlpI          470  GCTCAGC              GC^T[ACGT]AGC
BpmI          463  CTGGAG               CTGGAG(16/14)
Bpu1102I      470  GCTCAGC              GC^T[ACGT]AGC
BpuAI         229  GTCTTC               GAAGAC(2/6)
BsaHI         226  GACGTC               G[GA]^CG[CT]C
BseRI         571  CTCCTC               GAGGAG(10/8)
BsiHKAI       468  GTGCTC               G[AT]GC[AT]^C
BsiYI         426  CCTGGCACTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          426  CCTGGCACTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI         197  GTCTC                GTCTC(1/5)
BsmBI         196  CGTCTC               CGTCTC(1/5)
BsrBI         205  CCGCTC               CCGCTC(-3/-3)
BssHII        558  GCGCGC               G^CGCGC
BstAPI         44  GCAAGACCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII         470  GCTCAGC              GC^T[ACGT]AGC
DraII         422  AGGGCCT              [GA]G^G[ACGT]CC[CT]
DsaI           38  CCGTGG               C^C[GA][CT]GG
EagI          202  CGGCCG               C^GGCCG
EclXI         202  CGGCCG               C^GGCCG
Eco52I        202  CGGCCG               C^GGCCG
EcoO109I      422  AGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoT14I       437  CCAAGG               C^C[AT][AT]GG
Hin1I         226  GACGTC               G[GA]^CG[CT]C
HinfI         448  GACTC                G^A[ACGT]TC
MluNI         434  TGGCCA               TGG^CCA
MscI          434  TGGCCA               TGG^CCA
MslI          330  CATCCTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PleI          448  GACTC                GAGTC(4/5)
PshAI         222  GACGGACGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          564  CTGCAG               CTGCA^G
RsaI           93  GTAC                 GT^AC
SfcI          564  CTGCAG               C^T[GA][CT]AG
SfiI          513  GGCCATCCGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI          489  AGGCCT               AGG^CCT
StyI          437  CCAAGG               C^C[AT][AT]GG


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

BanI          305  GGCACC               G^G[CT][GA]CC
              340  GGCACC              
BbvI          549  GCAGC                GCAGC(8/12)
              566  GCAGC               
BcnI           85  CCCGG                CC^[GC]GG
              519  CCGGG               
BglI          514  GCCATCCGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              540  GCCCACGCGGC         
BsiEI         192  CGACCG               CG[GA][CT]^CG
              202  CGGCCG              
BsmFI         167  GGGAC                GGGAC(10/14)
              359  GGGAC               
BspMI          49  ACCTGC               ACCTGC(4/8)
              353  ACCTGC              
BsrI          432  ACTGG                ACTGG(1/-1)
              148  CCAGT               
BstXI         125  CCAATGTGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              326  CCATCATCCTGG        
DdeI          471  CTCAG                C^T[ACGT]AG
              536  CTCAG               
EaeI          202  CGGCCG               [CT]^GGCC[GA]
              434  TGGCCA              
FauI          417  CCCGC                CCCGC(4/6)
              357  GCGGG               
MaeII         227  ACGT                 A^CGT
              275  ACGT                
MboII         393  GAAGA                GAAGA(8/7)
              230  TCTTC               
NciI           85  CCCGG                CC^[GC]GG
              519  CCGGG               
TaqI          377  TCGA                 T^CGA
              506  TCGA                
TseI          549  GCAGC                G^C[AT]GC
              566  GCAGC               
TthHB8I       377  TCGA                 T^CGA
              506  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   33 enzymes) #
#########################################

AccII       AciI        AluI        BmyI        BsaJI       
Bsp1286I    BssKI       BstF5I      BstNI       BstUI       
Cac8I       CfoI        Cfr13I      EcoRII      Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HpaII       HphI        ItaI        MnlI        MspI        
MvaI        MvnI        MwoI        NlaIV       Sau96I      
ScrFI       TspRI       XcmI        
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