Restriction map for pF1KB8459
 (Linear) (Six-base) MAPSORT of: KIBB8459.seq  Check: 1774  from: 1  to: 1404



KIBB8459.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:06  ..

Acc65I G'GTAC_C

Cuts at:      0     195    1404 
   Size:        195    1209
 
AclI AA'CG_TT

Cuts at:      0     703    1220    1268    1404 
   Size:        703     517      48     136

  Fragments arranged by size: 

                 703     517     136      48
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      83     749    1404 
   Size:         83     666     655

  Fragments arranged by size: 

                 666     655      83
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1228    1404 
   Size:       1228     176
 
ApoI r'AATT_y

Cuts at:      0     618    1379    1404 
   Size:        618     761      25

  Fragments arranged by size: 

                 761     618      25
 
BanI G'GyrC_C

Cuts at:      0     195    1404 
   Size:        195    1209
 
BanII G_rGCy'C

Cuts at:      0     850    1404 
   Size:        850     554
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1174    1404 
   Size:       1174     230
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1294    1328    1404 
   Size:       1294      34      76

  Fragments arranged by size: 

                1294      76      34
 
Bme1580I G_kGCm'C

Cuts at:      0     291    1404 
   Size:        291    1113
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     182     224     260     882    1404 
   Size:        182      42      36     622     522

  Fragments arranged by size: 

                 622     522     182      42      36
 
BsaAI yAC'GTr

Cuts at:      0     522    1404 
   Size:        522     882
 
BsaBI GATnn'nnATC

Cuts at:      0     150    1404 
   Size:        150    1254
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     645     675    1404 
   Size:        645      30     729

  Fragments arranged by size: 

                 729     645      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     179     221     411     414    1404 
   Size:        179      42     190       3     990

  Fragments arranged by size: 

                 990     190     179      42       3
 
BsmI GAATG_Cn'

Cuts at:      0    1000    1317    1404 
   Size:       1000     317      87

  Fragments arranged by size: 

                1000     317      87
 
Bsp1286I G_dGCh'C

Cuts at:      0     291     850    1404 
   Size:        291     559     554

  Fragments arranged by size: 

                 559     554     291
 
BstYI r'GATC_y

Cuts at:      0     321    1404 
   Size:        321    1083
 
BtgI C'CryG_G

Cuts at:      0      83    1404 
   Size:         83    1321
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     354     389    1404 
   Size:        354      35    1015

  Fragments arranged by size: 

                1015     354      35
 
DraI TTT'AAA

Cuts at:      0     174     183     561    1119    1148    1404 
   Size:        174       9     378     558      29     256

  Fragments arranged by size: 

                 558     378     256     174      29       9
 
EaeI y'GGCC_r

Cuts at:      0       2      86     479    1404 
   Size:          2      84     393     925

  Fragments arranged by size: 

                 925     393      84       2
 
EarI CTCTTCn'nnn_

Cuts at:      0      59     945    1010    1404 
   Size:         59     886      65     394

  Fragments arranged by size: 

                 886     394      65      59
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      83     182     224     260     749     882    1404 
   Size:         83      99      42      36     489     133     522

  Fragments arranged by size: 

                 522     489     133      99      83      42      36
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     203     254    1404 
   Size:        203      51    1150

  Fragments arranged by size: 

                1150     203      51
 
EcoRI G'AATT_C

Cuts at:      0     618    1404 
   Size:        618     786
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     351    1404 
   Size:        351    1053
 
HaeII r_GCGC'y

Cuts at:      0     239    1404 
   Size:        239    1165
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     745     769     777     801    1404 
   Size:        745      24       8      24     603

  Fragments arranged by size: 

                 745     603      24      24       8
 
HincII GTy'rAC

Cuts at:      0     701    1404 
   Size:        701     703
 
HindIII A'AGCT_T

Cuts at:      0     446    1404 
   Size:        446     958
 
KpnI G_GTAC'C

Cuts at:      0     199    1404 
   Size:        199    1205
 
MfeI C'AATT_G

Cuts at:      0    1298    1404 
   Size:       1298     106
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     123     435    1404 
   Size:        123     312     969

  Fragments arranged by size: 

                 969     312     123
 
MscI TGG'CCA

Cuts at:      0       4      88    1404 
   Size:          4      84    1316

  Fragments arranged by size: 

                1316      84       4
 
MspA1I CmG'CkG

Cuts at:      0     316     649    1404 
   Size:        316     333     755

  Fragments arranged by size: 

                 755     333     316
 
NcoI C'CATG_G

Cuts at:      0      83    1404 
   Size:         83    1321
 
PmlI CAC'GTG

Cuts at:      0     522    1404 
   Size:        522     882
 
PvuII CAG'CTG

Cuts at:      0     649    1404 
   Size:        649     755
 
SfcI C'TryA_G

Cuts at:      0    1154    1404 
   Size:       1154     250
 
SspI AAT'ATT

Cuts at:      0     577    1404 
   Size:        577     827
 
StyI C'CwwG_G

Cuts at:      0      83     326     368    1404 
   Size:         83     243      42    1036

  Fragments arranged by size: 

                1036     243      83      42
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1047    1248    1404 
   Size:       1047     201     156

  Fragments arranged by size: 

                1047     201     156
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     960    1404 
   Size:        960     444
 

 Enzymes that do cut:

   Acc65I     AclI     AcuI     AlfI     ApoI     BanI    BanII     BbsI
     BcgI Bme1580I     BpmI    BsaAI    BsaBI    BsaXI    BseRI     BsmI
 Bsp1286I    BstYI     BtgI    CspCI     DraI     EaeI     EarI  Eco57MI
    EcoNI    EcoRI     FalI    HaeII    Hin4I   HincII  HindIII     KpnI
     MfeI     MmeI     MscI   MspA1I     NcoI     PmlI    PvuII     SfcI
     SspI     StyI    TaqII     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BbeI    BbvCI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI
   Bpu10I    BpuEI     BsaI    BsaHI    BsaWI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI   DraIII     DrdI     EagI
     EciI  EcoICRI EcoO109I    EcoRV     FseI     FspI    FspAI     HpaI
     KasI     MluI     MslI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI
     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
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