Restriction map for pF1KB8444
##################################
# NO CUT ENZYMES (  150 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BanI        BanII       BbeI        BbrPI       
BbvCI       BcgI        BclI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BseAI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrDI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstXI       
BstZ17I     Bsu36I      CelII       ClaI        DraII       
DraIII      DrdI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoO109I    
EcoO65I     EcoRV       FbaI        FseI        FspI        
HgaI        Hin1I       HincII      HindII      HpaI        
KasI        KpnI        Ksp632I     KspI        MamI        
MfeI        MluI        MroI        MspA1I      MunI        
NarI        NcoI        NdeI        NheI        NotI        
NruI        NspI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
PvuII       RcaI        SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        Tth111I     XbaI        
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AluI          539  AGCT                 AG^CT
AlwNI         602  CAGAACCTG            CAG[ACGT][ACGT][ACGT]^CTG
BalI          816  TGGCCA               TGG^CCA
BamHI         596  GGATCC               G^GATCC
BbsI          418  GAAGAC               GAAGAC(2/6)
BciVI         312  GTATCC               GTATCC(6/5)
BfaI          448  CTAG                 C^TAG
BmyI          485  GTGCCC               G[AGT]GC[ACT]^C
BpuAI         418  GAAGAC               GAAGAC(2/6)
BsaI          171  GGTCTC               GGTCTC(1/5)
BsaWI         658  TCCGGT               [AT]^CCGG[AT]
Bsp1286I      485  GTGCCC               G[AGT]GC[ACT]^C
BsrBI          30  CCGCTC               CCGCTC(-3/-3)
BsrI          819  CCAGT                ACTGG(1/-1)
CpoI           26  CGGACCG              CG^G[AT]CCG
DraI          430  TTTAAA               TTT^AAA
DsaI           96  CCACGG               C^C[GA][CT]GG
EcoNI           5  CCTCCGCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         579  GAATTC               G^AATTC
EcoT14I       764  CCTTGG               C^C[AT][AT]GG
EcoT22I       614  ATGCAT               ATGCA^T
FauI          168  GCGGG                CCCGC(4/6)
HaeII          92  AGCGCC               [GA]GCGC^[CT]
HindIII       538  AAGCTT               A^AGCTT
MaeI          448  CTAG                 C^TAG
MaeII         869  ACGT                 A^CGT
MluNI         816  TGGCCA               TGG^CCA
MscI          816  TGGCCA               TGG^CCA
MslI          798  CACAAGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI          243  GCCGGC               GCC^GGC
NgoMIV        243  GCCGGC               G^CCGGC
NsiI          614  ATGCAT               ATGCA^T
PflMI         270  CCACTACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI          650  CTGCAG               CTGCA^G
RsrII          26  CGGACCG              CG^G[AT]CCG
SfaNI         412  GATGC                GCATC(5/9)
StyI          764  CCTTGG               C^C[AT][AT]GG
Van91I        270  CCACTACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccII         115  CGCG                 CG^CG
              167  CGCG                
AcsI          229  GAATTT               [GA]^AATT[CT]
              579  GAATTC              
AfaI          382  GTAC                 GT^AC
              739  GTAC                
ApoI          229  GAATTT               [GA]^AATT[CT]
              579  GAATTC              
BbvI          673  GCAGC                GCAGC(8/12)
              195  GCTGC               
BpmI           82  CTCCAG               CTGGAG(16/14)
              253  CTGGAG              
BseRI          21  CTCCTC               GAGGAG(10/8)
              641  CTCCTC              
BsrFI          51  GCCGGT               [GA]^CCGG[CT]
              243  GCCGGC              
BstUI         115  CGCG                 CG^CG
              167  CGCG                
Cfr10I         51  GCCGGT               [GA]^CCGG[CT]
              243  GCCGGC              
DdeI          339  CTCAG                C^T[ACGT]AG
              629  CTCAG               
EaeI          501  TGGCCG               [CT]^GGCC[GA]
              816  TGGCCA              
Fnu4HI        195  GCTGC                GC^[ACGT]GC
              673  GCAGC               
ItaI          195  GCTGC                GC^[ACGT]GC
              673  GCAGC               
MaeIII        782  GTGAC                ^GT[ACGT]AC
              796  GTCAC               
MseI          212  TTAA                 T^TAA
              431  TTAA                
MvnI          115  CGCG                 CG^CG
              167  CGCG                
NlaIII        568  CATG                 CATG^
              613  CATG                
PleI           19  GACTC                GAGTC(4/5)
              387  GACTC               
RsaI          382  GTAC                 GT^AC
              739  GTAC                
SfcI          356  CTACAG               C^T[GA][CT]AG
              650  CTGCAG              
TfiI          118  GATTC                G^A[AT]TC
              516  GATTC               
Tru9I         212  TTAA                 T^TAA
              431  TTAA                
TseI          195  GCTGC                G^C[AT]GC
              673  GCAGC               
Tsp45I        782  GTGAC                ^GT[GC]AC
              796  GTCAC               
Tsp509I       230  AATT                 ^AATT
              580  AATT                
TspRI         475  CAGTG                CA[GC]TG[ACGT][ACGT]^
              820  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   43 enzymes) #
#########################################

AciI        AlwI        AvaII       BcnI        BsaJI       
BsiYI       BslI        BsmAI       BsmFI       BspMI       
BssKI       BstF5I      BstNI       BstYI       Cac8I       
CfoI        Cfr13I      DpnII       EcoRII      FokI        
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        MboI        MboII       MflI        
MnlI        MspI        MvaI        MwoI        NciI        
NdeII       NlaIV       Sau3AI      Sau96I      ScrFI       
TaqI        TthHB8I     XhoII       
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