Restriction map for
pF1KB8444
(Circular) (Six-base) MAPSORT of: pF1KB8444.seq Check: 1840 from: 1 to: 4022
pF1KB8444 4022 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 244 244
Size: 4022
AccI GT'mk_AC
Cuts at: 1060 1060
Size: 4022
Acc65I G'GTAC_C
Cuts at: 1038 1038
Size: 4022
AclI AA'CG_TT
Cuts at: 3862 3862
Size: 4022
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 596 1847 2279 2632 3294 596
Size: 1251 432 353 662 1324
Fragments arranged by size:
1324 1251 662 432 353
AflIII A'CryG_T
Cuts at: 1374 3174 3455 1374
Size: 1800 281 1941
Fragments arranged by size:
1941 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 715 1327 2765 715
Size: 612 1438 1972
Fragments arranged by size:
1972 1438 612
ApaLI G'TGCA_C
Cuts at: 2860 2860
Size: 4022
ApoI r'AATT_y
Cuts at: 337 687 1026 3375 3631 337
Size: 350 339 2349 256 728
Fragments arranged by size:
2349 728 350 339 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4022
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4022
AvaI C'yCGr_G
Cuts at: 1042 1042
Size: 4022
BamHI G'GATC_C
Cuts at: 704 1047 704
Size: 343 3679
BanI G'GyrC_C
Cuts at: 1038 1452 1700 1735 1038
Size: 414 248 35 3325
Fragments arranged by size:
3325 414 248 35
BanII G_rGCy'C
Cuts at: 1036 2066 1036
Size: 1030 2992
BbeI G_GCGC'C
Cuts at: 1456 1704 1456
Size: 248 3774
BbsI GAAGACnn'nnnn_
Cuts at: 533 533
Size: 4022
BciVI GTATCCnnnnn_n'
Cuts at: 431 1920 2976 4022 431
Size: 1489 1056 1046 431
Fragments arranged by size:
1489 1056 1046 431
BclI T'GATC_A
Cuts at: 1542 1542
Size: 4022
BfrBI ATG'CAT
Cuts at: 724 724
Size: 4022
BglII A'GATC_T
Cuts at: 1537 3574 1537
Size: 2037 1985
BlpI GC'TnA_GC
Cuts at: 1134 1134
Size: 4022
Bme1580I G_kGCm'C
Cuts at: 597 1647 1740 2864 597
Size: 1050 93 1124 1755
Fragments arranged by size:
1755 1124 1050 93
BmgBI CAC'GTC
Cuts at: 978 978
Size: 4022
BmrI ACTGGGnnnn_n'
Cuts at: 1234 1315 1650 3794 1234
Size: 81 335 2144 1462
Fragments arranged by size:
2144 1462 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2389 2389
Size: 4022
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 382 175
Size: 207 3815
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1198 2077 2791 3089 1198
Size: 879 714 298 2131
Fragments arranged by size:
2131 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 285 3726 285
Size: 3441 581
BsaAI yAC'GTr
Cuts at: 1375 1375
Size: 4022
BsaBI GATnn'nnATC
Cuts at: 6 1536 3573 6
Size: 1530 2037 455
Fragments arranged by size:
2037 1530 455
BsaHI Gr'CG_yC
Cuts at: 1453 1701 2403 3899 1453
Size: 248 702 1496 1576
Fragments arranged by size:
1576 1496 702 248
BsaWI w'CCGG_w
Cuts at: 766 1211 1732 2410 2821 2968 3242 766
Size: 445 521 678 411 147 274 1546
Fragments arranged by size:
1546 678 521 445 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 732 762 732
Size: 30 3992
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 120 740 120
Size: 620 3402
BseYI C'CCAG_C
Cuts at: 1447 2205 2870 3278 1447
Size: 758 665 408 2191
Fragments arranged by size:
2191 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 1564 1610 2840 104
Size: 1460 46 1230 1286
Fragments arranged by size:
1460 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1036 1814 2004 2864 1036
Size: 778 190 860 2194
Fragments arranged by size:
2194 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 597 1036 1647 1740 1814 2004 2066 2864 597
Size: 439 611 93 74 190 62 798 1755
Fragments arranged by size:
1755 798 611 439 190 93 74 62
BspHI T'CATG_A
Cuts at: 2454 2454
Size: 4022
BspMI ACCTGCnnnn'nnnn_
Cuts at: 244 467 539 723 765 1072 1286 1588 1969
Size: 223 72 184 42 307 214 302 381
Cuts at: 1969 244
Size: 2297
Fragments arranged by size:
2297 381 307 302 223 214 184 72
42
BsrBI CCG'CTC
Cuts at: 50 140 2314 2368 3852 4017 50
Size: 90 2174 54 1484 165 55
Fragments arranged by size:
2174 1484 165 90 55 54
BsrDI GCAATG_nn'
Cuts at: 1934 1934
Size: 4022
BsrFI r'CCGG_y
Cuts at: 159 351 2020 2201 159
Size: 192 1669 181 1980
Fragments arranged by size:
1980 1669 192 181
BssSI C'ACGA_G
Cuts at: 2293 3001 2293
Size: 708 3314
BstAPI GCAn_nnn'nTGC
Cuts at: 1399 1399
Size: 4022
BstBI TT'CG_AA
Cuts at: 2383 2383
Size: 4022
BstYI r'GATC_y
Cuts at: 1 374 439 551 655 704 1047 1514 1537
Size: 373 65 112 104 49 343 467 23
Cuts at: 1537 1872 2118 2424 2436 2522 3574 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 467 449 373 343 335 306 246
112 104 86 65 49 23 12
BtgI C'CryG_G
Cuts at: 204 204
Size: 4022
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1972 2153 2316 1972
Size: 181 163 3678
Fragments arranged by size:
3678 181 163
BtsI GCAGTG_nn'
Cuts at: 1265 1265
Size: 4022
DraI TTT'AAA
Cuts at: 540 1021 540
Size: 481 3541
DrdI GACnn_nn'nnGTC
Cuts at: 1728 3072 1728
Size: 1344 2678
EaeI y'GGCC_r
Cuts at: 609 924 1607 1781 2172 2199 3544 609
Size: 315 683 174 391 27 1345 1087
Fragments arranged by size:
1345 1087 683 391 315 174 27
EagI C'GGCC_G
Cuts at: 1607 1607
Size: 4022
EarI CTCTTCn'nnn_
Cuts at: 2045 2255 2045
Size: 210 3812
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 105 132 2230 2972 3118 3832 105
Size: 27 2098 742 146 714 295
Fragments arranged by size:
2098 742 714 295 146 27
EcoICRI GAG'CTC
Cuts at: 1034 1034
Size: 4022
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 382 596 1847 2279 2632 3294 175
Size: 207 214 1251 432 353 662 903
Fragments arranged by size:
1251 903 662 432 353 214 207
EcoNI CCTnn'n_nnAGG
Cuts at: 117 117
Size: 4022
EcoO109I rG'GnC_Cy
Cuts at: 1161 1161
Size: 4022
EcoRI G'AATT_C
Cuts at: 687 1026 687
Size: 339 3683
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 888 2041 888
Size: 1153 2869
FspI TGC'GCA
Cuts at: 1803 1803
Size: 4022
HaeII r_GCGC'y
Cuts at: 204 1456 1704 2934 3705 204
Size: 1252 248 1230 771 521
Fragments arranged by size:
1252 1230 771 521 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 732 764 1864 1894 1896 1926 732
Size: 32 1100 30 2 30 2828
Fragments arranged by size:
2828 1100 32 30 30 2
HincII GTy'rAC
Cuts at: 1061 1061
Size: 4022
HindIII A'AGCT_T
Cuts at: 646 646
Size: 4022
KasI G'GCGC_C
Cuts at: 1452 1700 1452
Size: 248 3774
KpnI G_GTAC'C
Cuts at: 1042 1042
Size: 4022
MluI A'CGCG_T
Cuts at: 3455 3455
Size: 4022
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 716 2781 2965 716
Size: 2065 184 1773
Fragments arranged by size:
2065 1773 184
MscI TGG'CCA
Cuts at: 926 1783 926
Size: 857 3165
MslI CAynn'nnrTG
Cuts at: 910 2011 2138 910
Size: 1101 127 2794
Fragments arranged by size:
2794 1101 127
MspA1I CmG'CkG
Cuts at: 1133 1447 1807 2591 2836 1133
Size: 314 360 784 245 2319
Fragments arranged by size:
2319 784 360 314 245
NaeI GCC'GGC
Cuts at: 353 2203 353
Size: 1850 2172
NarI GG'CG_CC
Cuts at: 1453 1701 1453
Size: 248 3774
NgoMIV G'CCGG_C
Cuts at: 351 2201 351
Size: 1850 2172
NsiI A_TGCA'T
Cuts at: 726 726
Size: 4022
NspI r_CATG'y
Cuts at: 1075 1575 3178 1075
Size: 500 1603 1919
Fragments arranged by size:
1919 1603 500
PasI CC'CwG_GG
Cuts at: 241 241
Size: 4022
PciI A'CATG_T
Cuts at: 3174 3174
Size: 4022
PflMI CCAn_nnn'nTGG
Cuts at: 384 384
Size: 4022
PmeI GTTT'AAAC
Cuts at: 1021 1021
Size: 4022
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2468 2500 2468
Size: 32 3990
PstI C_TGCA'G
Cuts at: 762 1069 1754 762
Size: 307 685 3030
Fragments arranged by size:
3030 685 307
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4022
PvuII CAG'CTG
Cuts at: 1447 1807 1447
Size: 360 3662
RsrII CG'GwC_CG
Cuts at: 135 2217 135
Size: 2082 1940
SacI G_AGCT'C
Cuts at: 1036 1036
Size: 4022
SalI G'TCGA_C
Cuts at: 1059 1059
Size: 4022
SapI GCTCTTCn'nnn_
Cuts at: 2045 2255 2045
Size: 210 3812
SbfI CC_TGCA'GG
Cuts at: 1069 1069
Size: 4022
ScaI AGT'ACT
Cuts at: 3539 3539
Size: 4022
SfcI C'TryA_G
Cuts at: 33 464 758 1065 1750 2718 2909 33
Size: 431 294 307 685 968 191 1146
Fragments arranged by size:
1146 968 685 431 307 294 191
SfoI GGC'GCC
Cuts at: 1454 1702 1454
Size: 248 3774
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4022
SmaI CCC'GGG
Cuts at: 1044 1044
Size: 4022
SmlI C'TyrA_G
Cuts at: 1177 2092 2806 3068 1177
Size: 915 714 262 2131
Fragments arranged by size:
2131 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4022
SphI G_CATG'C
Cuts at: 1075 1575 1075
Size: 500 3522
StyI C'CwwG_G
Cuts at: 872 1156 1510 872
Size: 284 354 3384
Fragments arranged by size:
3384 354 284
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 468 1605 1738 2197 2406 468
Size: 1137 133 459 209 2084
Fragments arranged by size:
2084 1137 459 209 133
TatI w'GTAC_w
Cuts at: 3537 3537
Size: 4022
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3539 3571 3539
Size: 32 3990
Tth111I GACn'n_nGTC
Cuts at: 1819 1819
Size: 4022
XbaI T'CTAG_A
Cuts at: 1053 1053
Size: 4022
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1517 1517
Size: 4022
XmaI C'CCGG_G
Cuts at: 1042 1042
Size: 4022
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NsiI NspI
PasI PciI PflMI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BglI BmtI
Bpu10I BsgI BsiWI BsmI BsmBI BspEI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HpaI MfeI NcoI NdeI NheI NotI
NruI PacI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI