Restriction map for pF1KB8436
 (Linear) (Six-base) MAPSORT of: KIBB8436.seq  Check: 9907  from: 1  to: 1077



KIBB8436.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:06  ..

AccI GT'mk_AC

Cuts at:      0     866    1077 
   Size:        866     211
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     949    1077 
   Size:        949     128
 
AfeI AGC'GCT

Cuts at:      0     692    1077 
   Size:        692     385
 
ApoI r'AATT_y

Cuts at:      0     919    1077 
   Size:        919     158
 
AvaI C'yCGr_G

Cuts at:      0     133     914    1077 
   Size:        133     781     163

  Fragments arranged by size: 

                 781     163     133
 
BanII G_rGCy'C

Cuts at:      0     119    1077 
   Size:        119     958
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     190     641    1077 
   Size:        190     451     436

  Fragments arranged by size: 

                 451     436     190
 
BclI T'GATC_A

Cuts at:      0     269     705    1077 
   Size:        269     436     372

  Fragments arranged by size: 

                 436     372     269
 
BglI GCCn_nnn'nGGC

Cuts at:      0     262    1077 
   Size:        262     815
 
BglII A'GATC_T

Cuts at:      0     828    1077 
   Size:        828     249
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      28    1030    1077 
   Size:         28    1002      47

  Fragments arranged by size: 

                1002      47      28
 
Bpu10I CC'TnA_GC

Cuts at:      0      31    1077 
   Size:         31    1046
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     103     274    1077 
   Size:        103     171     803

  Fragments arranged by size: 

                 803     171     103
 
BsaAI yAC'GTr

Cuts at:      0     986    1077 
   Size:        986      91
 
BsaBI GATnn'nnATC

Cuts at:      0     178    1077 
   Size:        178     899
 
BsaHI Gr'CG_yC

Cuts at:      0     104    1077 
   Size:        104     973
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     346     376    1077 
   Size:        346      30     701

  Fragments arranged by size: 

                 701     346      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      52     789     853    1077 
   Size:         52     737      64     224

  Fragments arranged by size: 

                 737     224      64      52
 
BsiHKAI G_wGCw'C

Cuts at:      0     119     314    1077 
   Size:        119     195     763

  Fragments arranged by size: 

                 763     195     119
 
BsmI GAATG_Cn'

Cuts at:      0     433    1077 
   Size:        433     644
 
Bsp1286I G_dGCh'C

Cuts at:      0     119     314    1077 
   Size:        119     195     763

  Fragments arranged by size: 

                 763     195     119
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     524    1077 
   Size:        524     553
 
BsrBI CCG'CTC

Cuts at:      0      78    1077 
   Size:         78     999
 
BssSI C'ACGA_G

Cuts at:      0     715    1077 
   Size:        715     362
 
BstYI r'GATC_y

Cuts at:      0     293     828    1077 
   Size:        293     535     249

  Fragments arranged by size: 

                 535     293     249
 
BtgI C'CryG_G

Cuts at:      0     186     278    1077 
   Size:        186      92     799

  Fragments arranged by size: 

                 799     186      92
 
BtsI GCAGTG_nn'

Cuts at:      0     651    1077 
   Size:        651     426
 
DrdI GACnn_nn'nnGTC

Cuts at:      0       9    1077 
   Size:          9    1068
 
EaeI y'GGCC_r

Cuts at:      0     254    1077 
   Size:        254     823
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      72    1077 
   Size:         72    1005
 
EcoICRI GAG'CTC

Cuts at:      0     117    1077 
   Size:        117     960
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      28     949    1030    1077 
   Size:         28     921      81      47

  Fragments arranged by size: 

                 921      81      47      28
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      34    1077 
   Size:         34    1043
 
EcoRI G'AATT_C

Cuts at:      0     919    1077 
   Size:        919     158
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     553     777    1077 
   Size:        553     224     300

  Fragments arranged by size: 

                 553     300     224
 
HaeII r_GCGC'y

Cuts at:      0     694    1077 
   Size:        694     383
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     346     378     533     565    1077 
   Size:        346      32     155      32     512

  Fragments arranged by size: 

                 512     346     155      32      32
 
HincII GTy'rAC

Cuts at:      0     867    1077 
   Size:        867     210
 
MslI CAynn'nnrTG

Cuts at:      0     256    1077 
   Size:        256     821
 
NcoI C'CATG_G

Cuts at:      0     186     278    1077 
   Size:        186      92     799

  Fragments arranged by size: 

                 799     186      92
 
NdeI CA'TA_TG

Cuts at:      0     381    1077 
   Size:        381     696
 
PfoI T'CCnGG_A

Cuts at:      0     414     422    1077 
   Size:        414       8     655

  Fragments arranged by size: 

                 655     414       8
 
PmlI CAC'GTG

Cuts at:      0     986    1077 
   Size:        986      91
 
SacI G_AGCT'C

Cuts at:      0     119    1077 
   Size:        119     958
 
SalI G'TCGA_C

Cuts at:      0     865    1077 
   Size:        865     212
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     262    1077 
   Size:        262     815
 
SmlI C'TyrA_G

Cuts at:      0     118     289    1077 
   Size:        118     171     788

  Fragments arranged by size: 

                 788     171     118
 
StyI C'CwwG_G

Cuts at:      0      44     186     278    1077 
   Size:         44     142      92     799

  Fragments arranged by size: 

                 799     142      92      44
 
TatI w'GTAC_w

Cuts at:      0    1064    1077 
   Size:       1064      13
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     278     310    1077 
   Size:        278      32     767

  Fragments arranged by size: 

                 767     278      32
 
XbaI T'CTAG_A

Cuts at:      0     834    1077 
   Size:        834     243
 
XmnI GAAnn'nnTTC

Cuts at:      0     247     923     934    1077 
   Size:        247     676      11     143

  Fragments arranged by size: 

                 676     247     143      11
 

 Enzymes that do cut:

     AccI     AcuI     AfeI     ApoI     AvaI    BanII    BciVI     BclI
     BglI    BglII     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI
    BsaXI    BseRI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrBI    BssSI
    BstYI     BtgI     BtsI     DrdI     EaeI     EciI  EcoICRI  Eco57MI
    EcoNI    EcoRI     FalI    HaeII    Hin4I   HincII     MslI     NcoI
     NdeI     PfoI     PmlI     SacI     SalI     SfiI     SmlI     StyI
     TatI     TstI     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI
     BcgI    BfrBI     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BsaI    BsaWI    BseYI     BsgI    BsiEI    BsiWI    BsmBI    BspEI
    BspHI    BsrDI    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII
     EagI     EarI EcoO109I    EcoRV     FseI     FspI    FspAI  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI   MspA1I
     NaeI     NarI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PmeI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
    SacII    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII  Tth111I     XcmI     XhoI     XmaI     ZraI
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