Restriction map for pF1KB8430
 (Circular) (Six-base) MAPSORT of: pF1KB8430.seq  Check: 463  from: 1  to: 3884



pF1KB8430  3884 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:28  ..

AccI GT'mk_AC

Cuts at:    922     922 
   Size:       3884
 
Acc65I G'GTAC_C

Cuts at:    900     900 
   Size:       3884
 
AclI AA'CG_TT

Cuts at:   3724    3724 
   Size:       3884
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    376    1709    2141    2494    3156     376 
   Size:       1333     432     353     662    1104

  Fragments arranged by size: 

                1333    1104     662     432     353
 
AflIII A'CryG_T

Cuts at:   1236    3036    3317    1236 
   Size:       1800     281    1803

  Fragments arranged by size: 

                1803    1800     281
 
AlwNI CAG_nnn'CTG

Cuts at:   1189    2627    1189 
   Size:       1438    2446
 
ApaLI G'TGCA_C

Cuts at:   2722    2722 
   Size:       3884
 
ApoI r'AATT_y

Cuts at:    405     888    3237    3493     405 
   Size:        483    2349     256     796

  Fragments arranged by size: 

                2349     796     483     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3884
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3884
 
AvaI C'yCGr_G

Cuts at:    904     904 
   Size:       3884
 
BamHI G'GATC_C

Cuts at:    909     909 
   Size:       3884
 
BanI G'GyrC_C

Cuts at:    900    1314    1562    1597     900 
   Size:        414     248      35    3187

  Fragments arranged by size: 

                3187     414     248      35
 
BanII G_rGCy'C

Cuts at:    533     898    1928     533 
   Size:        365    1030    2489

  Fragments arranged by size: 

                2489    1030     365
 
BbeI G_GCGC'C

Cuts at:   1318    1566    1318 
   Size:        248    3636
 
BbsI GAAGACnn'nnnn_

Cuts at:    188     188 
   Size:       3884
 
BciVI GTATCCnnnnn_n'

Cuts at:   1782    2838    3884    1782 
   Size:       1056    1046    1782

  Fragments arranged by size: 

                1782    1056    1046
 
BclI T'GATC_A

Cuts at:   1404    1404 
   Size:       3884
 
BglII A'GATC_T

Cuts at:    326     606    1399    3436     326 
   Size:        280     793    2037     774

  Fragments arranged by size: 

                2037     793     774     280
 
BlpI GC'TnA_GC

Cuts at:    996     996 
   Size:       3884
 
Bme1580I G_kGCm'C

Cuts at:   1509    1602    2726    1509 
   Size:         93    1124    2667

  Fragments arranged by size: 

                2667    1124      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1096    1177    1512    3656    1096 
   Size:         81     335    2144    1324

  Fragments arranged by size: 

                2144    1324     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2251    2251 
   Size:       3884
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    448     698     713     448 
   Size:        250      15    3619

  Fragments arranged by size: 

                3619     250      15
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1060    1939    2653    2951    1060 
   Size:        879     714     298    1993

  Fragments arranged by size: 

                1993     879     714     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3588    3588 
   Size:       3884
 
BsaAI yAC'GTr

Cuts at:   1237    1237 
   Size:       3884
 
BsaBI GATnn'nnATC

Cuts at:      6    1398    3435       6 
   Size:       1392    2037     455

  Fragments arranged by size: 

                2037    1392     455
 
BsaHI Gr'CG_yC

Cuts at:   1315    1563    2265    3761    1315 
   Size:        248     702    1496    1438

  Fragments arranged by size: 

                1496    1438     702     248
 
BsaWI w'CCGG_w

Cuts at:   1073    1594    2272    2683    2830    3104    1073 
   Size:        521     678     411     147     274    1853

  Fragments arranged by size: 

                1853     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:   1309    2067    2732    3140    1309 
   Size:        758     665     408    2053

  Fragments arranged by size: 

                2053     758     665     408
 
BsiEI CG_ry'CG

Cuts at:    104     125    1426    1472    2702     104 
   Size:         21    1301      46    1230    1286

  Fragments arranged by size: 

                1301    1286    1230      46      21
 
BsiHKAI G_wGCw'C

Cuts at:    898    1676    1866    2726     898 
   Size:        778     190     860    2056

  Fragments arranged by size: 

                2056     860     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    533     898    1509    1602    1676    1866    1928    2726     533 
   Size:        365     611      93      74     190      62     798    1691

  Fragments arranged by size: 

                1691     798     611     365     190      93      74      62
            
 
BspHI T'CATG_A

Cuts at:   2316    2316 
   Size:       3884
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    934    1148    1450    1831     934 
   Size:        214     302     381    2987

  Fragments arranged by size: 

                2987     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2176    2230    3714    3879      50 
   Size:       2126      54    1484     165      55

  Fragments arranged by size: 

                2126    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1796    1796 
   Size:       3884
 
BsrFI r'CCGG_y

Cuts at:   1882    2063    1882 
   Size:        181    3703
 
BssSI C'ACGA_G

Cuts at:   2155    2863    2155 
   Size:        708    3176
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1261    1261 
   Size:       3884
 
BstBI TT'CG_AA

Cuts at:   2245    2245 
   Size:       3884
 
BstYI r'GATC_y

Cuts at:      1     326     606     909    1376    1399    1734    1980    2286 
   Size:        325     280     303     467      23     335     246     306

Cuts at:   2286    2298    2384    3436       1 
   Size:         12      86    1052     449

  Fragments arranged by size: 

                1052     467     449     335     325     306     303     280
                 246      86      23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       3884
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1834    2015    2178    1834 
   Size:        181     163    3540

  Fragments arranged by size: 

                3540     181     163
 
BtsI GCAGTG_nn'

Cuts at:    407     770    1127     407 
   Size:        363     357    3164

  Fragments arranged by size: 

                3164     363     357
 
DraI TTT'AAA

Cuts at:    883     883 
   Size:       3884
 
DrdI GACnn_nn'nnGTC

Cuts at:   1590    2934    1590 
   Size:       1344    2540
 
EaeI y'GGCC_r

Cuts at:   1469    1643    2034    2061    3406    1469 
   Size:        174     391      27    1345    1947

  Fragments arranged by size: 

                1947    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1469    1469 
   Size:       3884
 
EarI CTCTTCn'nnn_

Cuts at:    270    1907    2117     270 
   Size:       1637     210    2037

  Fragments arranged by size: 

                2037    1637     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2092    2834    2980    3694    2092 
   Size:        742     146     714    2282

  Fragments arranged by size: 

                2282     742     714     146
 
EcoICRI GAG'CTC

Cuts at:    896     896 
   Size:       3884
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    376     448     698     713    1709    2141    2494    3156     376 
   Size:         72     250      15     996     432     353     662    1104

  Fragments arranged by size: 

                1104     996     662     432     353     250      72      15
            
 
EcoO109I rG'GnC_Cy

Cuts at:   1023    1023 
   Size:       3884
 
EcoRI G'AATT_C

Cuts at:    888     888 
   Size:       3884
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1903    1903 
   Size:       3884
 
FspI TGC'GCA

Cuts at:   1665    1665 
   Size:       3884
 
HaeII r_GCGC'y

Cuts at:   1318    1566    2796    3567    1318 
   Size:        248    1230     771    1635

  Fragments arranged by size: 

                1635    1230     771     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    776     808    1726    1756    1758    1788     776 
   Size:         32     918      30       2      30    2872

  Fragments arranged by size: 

                2872     918      32      30      30       2
 
HincII GTy'rAC

Cuts at:    923     923 
   Size:       3884
 
KasI G'GCGC_C

Cuts at:   1314    1562    1314 
   Size:        248    3636
 
KpnI G_GTAC'C

Cuts at:    904     904 
   Size:       3884
 
MluI A'CGCG_T

Cuts at:   3317    3317 
   Size:       3884
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    232    2643    2827     232 
   Size:       2411     184    1289

  Fragments arranged by size: 

                2411    1289     184
 
MscI TGG'CCA

Cuts at:   1645    1645 
   Size:       3884
 
MslI CAynn'nnrTG

Cuts at:    466    1873    2000     466 
   Size:       1407     127    2350

  Fragments arranged by size: 

                2350    1407     127
 
MspA1I CmG'CkG

Cuts at:    808     995    1309    1669    2453    2698     808 
   Size:        187     314     360     784     245    1994

  Fragments arranged by size: 

                1994     784     360     314     245     187
 
NaeI GCC'GGC

Cuts at:   2065    2065 
   Size:       3884
 
NarI GG'CG_CC

Cuts at:   1315    1563    1315 
   Size:        248    3636
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3884
 
NgoMIV G'CCGG_C

Cuts at:   2063    2063 
   Size:       3884
 
NspI r_CATG'y

Cuts at:    937    1437    3040     937 
   Size:        500    1603    1781

  Fragments arranged by size: 

                1781    1603     500
 
PasI CC'CwG_GG

Cuts at:    481     481 
   Size:       3884
 
PciI A'CATG_T

Cuts at:   3036    3036 
   Size:       3884
 
PmeI GTTT'AAAC

Cuts at:    883     883 
   Size:       3884
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2330    2362    2330 
   Size:         32    3852
 
PstI C_TGCA'G

Cuts at:    931    1616     931 
   Size:        685    3199
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3884
 
PvuII CAG'CTG

Cuts at:    808    1309    1669     808 
   Size:        501     360    3023

  Fragments arranged by size: 

                3023     501     360
 
RsrII CG'GwC_CG

Cuts at:   2079    2079 
   Size:       3884
 
SacI G_AGCT'C

Cuts at:    898     898 
   Size:       3884
 
SalI G'TCGA_C

Cuts at:    921     921 
   Size:       3884
 
SapI GCTCTTCn'nnn_

Cuts at:   1907    2117    1907 
   Size:        210    3674
 
SbfI CC_TGCA'GG

Cuts at:    931     931 
   Size:       3884
 
ScaI AGT'ACT

Cuts at:   3401    3401 
   Size:       3884
 
SfcI C'TryA_G

Cuts at:     33     600     927    1612    2580    2771      33 
   Size:        567     327     685     968     191    1146

  Fragments arranged by size: 

                1146     968     685     567     327     191
 
SfoI GGC'GCC

Cuts at:   1316    1564    1316 
   Size:        248    3636
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3884
 
SmaI CCC'GGG

Cuts at:    906     906 
   Size:       3884
 
SmlI C'TyrA_G

Cuts at:   1039    1954    2668    2930    1039 
   Size:        915     714     262    1993

  Fragments arranged by size: 

                1993     915     714     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3884
 
SphI G_CATG'C

Cuts at:    937    1437     937 
   Size:        500    3384
 
SspI AAT'ATT

Cuts at:    392     392 
   Size:       3884
 
StyI C'CwwG_G

Cuts at:    107     255    1018    1372     107 
   Size:        148     763     354    2619

  Fragments arranged by size: 

                2619     763     354     148
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1467    1600    2059    2268    1467 
   Size:        133     459     209    3083

  Fragments arranged by size: 

                3083     459     209     133
 
TatI w'GTAC_w

Cuts at:   3399    3399 
   Size:       3884
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    690     722    3401    3433     690 
   Size:         32    2679      32    1141

  Fragments arranged by size: 

                2679    1141      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1681    1681 
   Size:       3884
 
XbaI T'CTAG_A

Cuts at:    915     915 
   Size:       3884
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1379    1379 
   Size:       3884
 
XmaI C'CCGG_G

Cuts at:    904     904 
   Size:       3884
 
XmnI GAAnn'nnTTC

Cuts at:    264     737     264 
   Size:        473    3411
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII    AlwNI    ApaLI     ApoI
     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI     BbsI
    BciVI     BclI    BglII     BlpI Bme1580I     BmrI     BplI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI    BsiEI
  BsiHKAI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI
   BstAPI    BstBI    BstYI     BtgI    BtgZI     BtsI     DraI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI
     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NspI     PasI     PciI     PmeI     PpiI     PstI     PvuI    PvuII
    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     SspI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AgeI     AhdI     AleI     AlfI
     AloI     ApaI     AscI    AvrII     BaeI    BbvCI     BcgI    BfrBI
     BglI    BmgBI     BmtI   Bpu10I    BsaXI    BseRI     BsgI    BsiWI
     BsmI    BsmBI    BspEI    BsrGI   BssHII   BstEII    BstXI  BstZ17I
   Bsu36I     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI
  HindIII     HpaI     MfeI     NdeI     NheI     NotI     NruI     NsiI
     PacI    PflMI     PfoI     PmlI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI
     SrfI     StuI     SwaI     XhoI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com