Restriction map for pF1KB8416
 (Circular) (Six-base) MAPSORT of: pF1KB8416.seq  Check: 6066  from: 1  to: 6269



pF1KB8416  6269 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:28  ..

AccI GT'mk_AC

Cuts at:    243    1838    3307     243 
   Size:       1595    1469    3205

  Fragments arranged by size: 

                3205    1595    1469
 
Acc65I G'GTAC_C

Cuts at:   3285    3285 
   Size:       6269
 
AclI AA'CG_TT

Cuts at:   6109    6109 
   Size:       6269
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    485    1928    2239    2365    2617    2945    3231    4094    4526 
   Size:       1443     311     126     252     328     286     863     432

Cuts at:   4526    4879    5541     485 
   Size:        353     662    1213

  Fragments arranged by size: 

                1443    1213     863     662     432     353     328     311
                 286     252     126
 
AflII C'TTAA_G

Cuts at:    303    1443     303 
   Size:       1140    5129
 
AflIII A'CryG_T

Cuts at:   3621    5421    5702    3621 
   Size:       1800     281    4188

  Fragments arranged by size: 

                4188    1800     281
 
AlwNI CAG_nnn'CTG

Cuts at:    214    1479    1600    2812    3574    5012     214 
   Size:       1265     121    1212     762    1438    1471

  Fragments arranged by size: 

                1471    1438    1265    1212     762     121
 
ApaLI G'TGCA_C

Cuts at:   5107    5107 
   Size:       6269
 
ApoI r'AATT_y

Cuts at:    189     677     712     752    1333    1411    1485    1539    1704 
   Size:        488      35      40     581      78      74      54     165

Cuts at:   1704    2080    2344    2488    3273    5622    5878     189 
   Size:        376     264     144     785    2349     256     580

  Fragments arranged by size: 

                2349     785     581     580     488     376     264     256
                 165     144      78      74      54      40      35
 
AseI AT'TA_AT

Cuts at:     20     857      20 
   Size:        837    5432
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6269
 
AvaI C'yCGr_G

Cuts at:   3289    3289 
   Size:       6269
 
BamHI G'GATC_C

Cuts at:    115    1953    3294     115 
   Size:       1838    1341    3090

  Fragments arranged by size: 

                3090    1838    1341
 
BanI G'GyrC_C

Cuts at:   1068    3285    3699    3947    3982    1068 
   Size:       2217     414     248      35    3355

  Fragments arranged by size: 

                3355    2217     414     248      35
 
BanII G_rGCy'C

Cuts at:   3283    4313    3283 
   Size:       1030    5239
 
BbeI G_GCGC'C

Cuts at:   3703    3951    3703 
   Size:        248    6021
 
BbsI GAAGACnn'nnnn_

Cuts at:   2187    2187 
   Size:       6269
 
BciVI GTATCCnnnnn_n'

Cuts at:   4167    5223    6269    4167 
   Size:       1056    1046    4167

  Fragments arranged by size: 

                4167    1056    1046
 
BclI T'GATC_A

Cuts at:   2667    3789    2667 
   Size:       1122    5147
 
BfrBI ATG'CAT

Cuts at:    232    3135     232 
   Size:       2903    3366
 
BglII A'GATC_T

Cuts at:    546    3784    5821     546 
   Size:       3238    2037     994

  Fragments arranged by size: 

                3238    2037     994
 
BlpI GC'TnA_GC

Cuts at:   1851    3381    1851 
   Size:       1530    4739
 
Bme1580I G_kGCm'C

Cuts at:    643    1894    3894    3987    5111     643 
   Size:       1251    2000      93    1124    1801

  Fragments arranged by size: 

                2000    1801    1251    1124      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   2193    3481    3562    3897    6041    2193 
   Size:       1288      81     335    2144    2421

  Fragments arranged by size: 

                2421    2144    1288     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4636    4636 
   Size:       6269
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    844    1033    3179    3215     844 
   Size:        189    2146      36    3898

  Fragments arranged by size: 

                3898    2146     189      36
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3445    4324    5038    5336    3445 
   Size:        879     714     298    4378

  Fragments arranged by size: 

                4378     879     714     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    532    1750    5973     532 
   Size:       1218    4223     828

  Fragments arranged by size: 

                4223    1218     828
 
BsaAI yAC'GTr

Cuts at:   1836    3622    1836 
   Size:       1786    4483
 
BsaBI GATnn'nnATC

Cuts at:      6    2666    3783    5820       6 
   Size:       2660    1117    2037     455

  Fragments arranged by size: 

                2660    2037    1117     455
 
BsaHI Gr'CG_yC

Cuts at:    151    3700    3948    4650    6146     151 
   Size:       3549     248     702    1496     274

  Fragments arranged by size: 

                3549    1496     702     274     248
 
BsaWI w'CCGG_w

Cuts at:   3458    3979    4657    5068    5215    5489    3458 
   Size:        521     678     411     147     274    4238

  Fragments arranged by size: 

                4238     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1006    1036    1006 
   Size:         30    6239
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1439    1439 
   Size:       6269
 
BseYI C'CCAG_C

Cuts at:   3694    4452    5117    5525    3694 
   Size:        758     665     408    4438

  Fragments arranged by size: 

                4438     758     665     408
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    683     683 
   Size:       6269
 
BsiEI CG_ry'CG

Cuts at:    104    3811    3857    5087     104 
   Size:       3707      46    1230    1286

  Fragments arranged by size: 

                3707    1286    1230      46
 
BsiHKAI G_wGCw'C

Cuts at:   3283    4061    4251    5111    3283 
   Size:        778     190     860    4441

  Fragments arranged by size: 

                4441     860     778     190
 
BsmI GAATG_Cn'

Cuts at:   2391    2580    2391 
   Size:        189    6080
 
Bsp1286I G_dGCh'C

Cuts at:    643    1894    3283    3894    3987    4061    4251    4313    5111 
   Size:       1251    1389     611      93      74     190      62     798

Cuts at:   5111     643 
   Size:       1801

  Fragments arranged by size: 

                1801    1389    1251     798     611     190      93      74
                  62
 
BspHI T'CATG_A

Cuts at:    378    3252    4701     378 
   Size:       2874    1449    1946

  Fragments arranged by size: 

                2874    1946    1449
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   2790    3319    3533    3835    4216    2790 
   Size:        529     214     302     381    4843

  Fragments arranged by size: 

                4843     529     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    4561    4615    6099    6264      50 
   Size:       4511      54    1484     165      55

  Fragments arranged by size: 

                4511    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    278     601    4181     278 
   Size:        323    3580    2366

  Fragments arranged by size: 

                3580    2366     323
 
BsrFI r'CCGG_y

Cuts at:   4267    4448    4267 
   Size:        181    6088
 
BsrGI T'GTAC_A

Cuts at:    669    2723     669 
   Size:       2054    4215
 
BssSI C'ACGA_G

Cuts at:   1421    4540    5248    1421 
   Size:       3119     708    2442

  Fragments arranged by size: 

                3119    2442     708
 
BstAPI GCAn_nnn'nTGC

Cuts at:   3646    3646 
   Size:       6269
 
BstBI TT'CG_AA

Cuts at:   4630    4630 
   Size:       6269
 
BstXI CCAn_nnnn'nTGG

Cuts at:    942     942 
   Size:       6269
 
BstYI r'GATC_y

Cuts at:      1     115     405     546     880    1596    1953    3174    3294 
   Size:        114     290     141     334     716     357    1221     120

Cuts at:   3294    3761    3784    4119    4365    4671    4683    4769    5821 
   Size:        467      23     335     246     306      12      86    1052

Cuts at:   5821       1 
   Size:        449

  Fragments arranged by size: 

                1221    1052     716     467     449     357     335     334
                 306     290     246     141     120     114      86      23
                  12
 
Bsu36I CC'TnA_GG

Cuts at:   1064    1064 
   Size:       6269
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       6269
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   4219    4400    4563    4219 
   Size:        181     163    5925

  Fragments arranged by size: 

                5925     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1676    2565    2648    2798    3512    1676 
   Size:        889      83     150     714    4433

  Fragments arranged by size: 

                4433     889     714     150      83
 
ClaI AT'CG_AT

Cuts at:    224    2976     224 
   Size:       2752    3517
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:   3044    3079    3044 
   Size:         35    6234
 
DraI TTT'AAA

Cuts at:    875    1490    2354    3268     875 
   Size:        615     864     914    3876

  Fragments arranged by size: 

                3876     914     864     615
 
DraIII CAC_nnn'GTG

Cuts at:   1469    1469 
   Size:       6269
 
DrdI GACnn_nn'nnGTC

Cuts at:   3975    5319    3975 
   Size:       1344    4925
 
EaeI y'GGCC_r

Cuts at:    128     284    3854    4028    4419    4446    5791     128 
   Size:        156    3570     174     391      27    1345     606

  Fragments arranged by size: 

                3570    1345     606     391     174     156      27
 
EagI C'GGCC_G

Cuts at:   3854    3854 
   Size:       6269
 
EarI CTCTTCn'nnn_

Cuts at:    140    1199    1958    2258    2426    4292    4502     140 
   Size:       1059     759     300     168    1866     210    1907

  Fragments arranged by size: 

                1907    1866    1059     759     300     210     168
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   4477    5219    5365    6079    4477 
   Size:        742     146     714    4667

  Fragments arranged by size: 

                4667     742     714     146
 
EcoICRI GAG'CTC

Cuts at:   3281    3281 
   Size:       6269
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    485     844    1033    1928    2239    2365    2617    2945    3179 
   Size:        359     189     895     311     126     252     328     234

Cuts at:   3179    3215    3231    4094    4526    4879    5541     485 
   Size:         36      16     863     432     353     662    1213

  Fragments arranged by size: 

                1213     895     863     662     432     359     353     328
                 311     252     234     189     126      36      16
 
EcoO109I rG'GnC_Cy

Cuts at:   3226    3408    3226 
   Size:        182    6087
 
EcoRI G'AATT_C

Cuts at:   2488    3273    2488 
   Size:        785    5484
 
EcoRV GAT'ATC

Cuts at:    905     905 
   Size:       6269
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    490    4288     490 
   Size:       3798    2471
 
FspI TGC'GCA

Cuts at:   4050    4050 
   Size:       6269
 
HaeII r_GCGC'y

Cuts at:   3703    3951    5181    5952    3703 
   Size:        248    1230     771    4020

  Fragments arranged by size: 

                4020    1230     771     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    303     335     531     563    2425    2457    4111    4141    4143 
   Size:         32     196      32    1862      32    1654      30       2

Cuts at:   4143    4173     303 
   Size:         30    2399

  Fragments arranged by size: 

                2399    1862    1654     196      32      32      32      30
                  30       2
 
HincII GTy'rAC

Cuts at:    206     244    3128    3308     206 
   Size:         38    2884     180    3167

  Fragments arranged by size: 

                3167    2884     180      38
 
HindIII A'AGCT_T

Cuts at:   2864    2864 
   Size:       6269
 
HpaI GTT'AAC

Cuts at:   3128    3128 
   Size:       6269
 
KasI G'GCGC_C

Cuts at:   3699    3947    3699 
   Size:        248    6021
 
KpnI G_GTAC'C

Cuts at:   3289    3289 
   Size:       6269
 
MfeI C'AATT_G

Cuts at:   2900    2900 
   Size:       6269
 
MluI A'CGCG_T

Cuts at:   5702    5702 
   Size:       6269
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1122    1643    1771    2610    5028    5212    1122 
   Size:        521     128     839    2418     184    2179

  Fragments arranged by size: 

                2418    2179     839     521     184     128
 
MscI TGG'CCA

Cuts at:    286    4030     286 
   Size:       3744    2525
 
MslI CAynn'nnrTG

Cuts at:    271    1090    4258    4385     271 
   Size:        819    3168     127    2155

  Fragments arranged by size: 

                3168    2155     819     127
 
MspA1I CmG'CkG

Cuts at:   2002    2698    2740    3380    3694    4054    4838    5083    2002 
   Size:        696      42     640     314     360     784     245    3188

  Fragments arranged by size: 

                3188     784     696     640     360     314     245      42
            
 
NaeI GCC'GGC

Cuts at:   4450    4450 
   Size:       6269
 
NarI GG'CG_CC

Cuts at:   3700    3948    3700 
   Size:        248    6021
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       6269
 
NgoMIV G'CCGG_C

Cuts at:   4448    4448 
   Size:       6269
 
NsiI A_TGCA'T

Cuts at:    234    3137     234 
   Size:       2903    3366
 
NspI r_CATG'y

Cuts at:   3322    3822    5425    3322 
   Size:        500    1603    4166

  Fragments arranged by size: 

                4166    1603     500
 
PciI A'CATG_T

Cuts at:   5421    5421 
   Size:       6269
 
PflMI CCAn_nnn'nTGG

Cuts at:   1141    2024    1141 
   Size:        883    5386
 
PmeI GTTT'AAAC

Cuts at:   3268    3268 
   Size:       6269
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4715    4747    4715 
   Size:         32    6237
 
PpuMI rG'GwC_Cy

Cuts at:   3226    3226 
   Size:       6269
 
PsiI TTA'TAA

Cuts at:   2164    2164 
   Size:       6269
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:    938     970    2003    2035     938 
   Size:         32    1033      32    5172

  Fragments arranged by size: 

                5172    1033      32      32
 
PstI C_TGCA'G

Cuts at:   1284    2106    3316    4001    1284 
   Size:        822    1210     685    3552

  Fragments arranged by size: 

                3552    1210     822     685
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       6269
 
PvuII CAG'CTG

Cuts at:   2002    2698    2740    3694    4054    2002 
   Size:        696      42     954     360    4217

  Fragments arranged by size: 

                4217     954     696     360      42
 
RsrII CG'GwC_CG

Cuts at:   4464    4464 
   Size:       6269
 
SacI G_AGCT'C

Cuts at:   3283    3283 
   Size:       6269
 
SalI G'TCGA_C

Cuts at:    242    3306     242 
   Size:       3064    3205
 
SapI GCTCTTCn'nnn_

Cuts at:   2258    4292    4502    2258 
   Size:       2034     210    4025

  Fragments arranged by size: 

                4025    2034     210
 
SbfI CC_TGCA'GG

Cuts at:   3316    3316 
   Size:       6269
 
ScaI AGT'ACT

Cuts at:   5786    5786 
   Size:       6269
 
SfcI C'TryA_G

Cuts at:     33    1280    2102    2405    2786    2804    3312    3997    4965 
   Size:       1247     822     303     381      18     508     685     968

Cuts at:   4965    5156      33 
   Size:        191    1146

  Fragments arranged by size: 

                1247    1146     968     822     685     508     381     303
                 191      18
 
SfoI GGC'GCC

Cuts at:   3701    3949    3701 
   Size:        248    6021
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6269
 
SmaI CCC'GGG

Cuts at:   3291    3291 
   Size:       6269
 
SmlI C'TyrA_G

Cuts at:    303    1443    3424    4339    5053    5315     303 
   Size:       1140    1981     915     714     262    1257

  Fragments arranged by size: 

                1981    1257    1140     915     714     262
 
SnaBI TAC'GTA

Cuts at:   1836    1836 
   Size:       6269
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       6269
 
SphI G_CATG'C

Cuts at:   3322    3822    3322 
   Size:        500    5769
 
SspI AAT'ATT

Cuts at:   2236    2463    2236 
   Size:        227    6042
 
StuI AGG'CCT

Cuts at:   1866    1866 
   Size:       6269
 
StyI C'CwwG_G

Cuts at:    107    1100    1199    1257    2825    3403    3757     107 
   Size:        993      99      58    1568     578     354    2619

  Fragments arranged by size: 

                2619    1568     993     578     354      99      58
 
SwaI ATTT'AAAT

Cuts at:   2354    2354 
   Size:       6269
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    304    3852    3985    4444    4653     304 
   Size:       3548     133     459     209    1920

  Fragments arranged by size: 

                3548    1920     459     209     133
 
TatI w'GTAC_w

Cuts at:    471     669    1981    2723    5784     471 
   Size:        198    1312     742    3061     956

  Fragments arranged by size: 

                3061    1312     956     742     198
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5786    5818    5786 
   Size:         32    6237
 
Tth111I GACn'n_nGTC

Cuts at:   1932    4066    1932 
   Size:       2134    4135
 
XbaI T'CTAG_A

Cuts at:   3300    3300 
   Size:       6269
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   3764    3764 
   Size:       6269
 
XmaI C'CCGG_G

Cuts at:   3289    3289 
   Size:       6269
 
XmnI GAAnn'nnTTC

Cuts at:   1335    1412    1335 
   Size:         77    6192
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI    AflII   AflIII    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I     BmrI
     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI
    BstBI    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI     FspI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NsiI     NspI     PciI    PflMI     PmeI     PpiI    PpuMI     PsiI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI
     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI    SnaBI
     SpeI     SphI     SspI     StuI     StyI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI     AgeI     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI    BbvCI     BcgI     BglI    BmgBI
     BmtI   Bpu10I    BsiWI    BsmBI    BspEI   BssHII   BstEII  BstZ17I
    EcoNI     FseI    FspAI     NdeI     NheI     NotI     NruI     PacI
     PasI     PfoI     PmlI    PshAI   PspOMI    PspXI    SacII    SanDI
    SexAI     SfiI    SgrAI     SrfI     XhoI     ZraI
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