Restriction map for
pF1KB8404
(Circular) (Six-base) MAPSORT of: pF1KB8404.seq Check: 8685 from: 1 to: 6653
pF1KB8404 6653 bp
With 182 enzymes: SgfI *
August 7, 2008 14:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 606 922 606
Size: 316 6337
AccI GT'mk_AC
Cuts at: 395 2060 3691 395
Size: 1665 1631 3357
Fragments arranged by size:
3357 1665 1631
Acc65I G'GTAC_C
Cuts at: 760 3669 760
Size: 2909 3744
AclI AA'CG_TT
Cuts at: 2642 6493 2642
Size: 3851 2802
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 3595 4478 4910 5263 5925 3595
Size: 883 432 353 662 4323
Fragments arranged by size:
4323 883 662 432 353
AflIII A'CryG_T
Cuts at: 954 1516 2045 2538 2671 4005 5805 6086 954
Size: 562 529 493 133 1334 1800 281 1521
Fragments arranged by size:
1800 1521 1334 562 529 493 281 133
AhdI GACnn_n'nnGTC
Cuts at: 1661 1661
Size: 6653
AleI CACnn'nnGTG
Cuts at: 419 3118 3343 419
Size: 2699 225 3729
Fragments arranged by size:
3729 2699 225
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 329 437 2408 3039 3246 329
Size: 108 1971 631 207 3736
Fragments arranged by size:
3736 1971 631 207 108
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 670 702 670
Size: 32 6621
AlwNI CAG_nnn'CTG
Cuts at: 474 3193 3239 3958 5396 474
Size: 2719 46 719 1438 1731
Fragments arranged by size:
2719 1731 1438 719 46
ApaI G_GGCC'C
Cuts at: 1057 1106 1262 2728 3379 1057
Size: 49 156 1466 651 4331
Fragments arranged by size:
4331 1466 651 156 49
ApaLI G'TGCA_C
Cuts at: 5491 5491
Size: 6653
ApoI r'AATT_y
Cuts at: 3657 6006 6262 3657
Size: 2349 256 4048
Fragments arranged by size:
4048 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6653
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6653
AvaI C'yCGr_G
Cuts at: 1443 2081 2339 3379 3673 1443
Size: 638 258 1040 294 4423
Fragments arranged by size:
4423 1040 638 294 258
BamHI G'GATC_C
Cuts at: 2942 3678 2942
Size: 736 5917
BanI G'GyrC_C
Cuts at: 760 1345 1850 1933 2281 2604 2616 2703 2826
Size: 585 505 83 348 323 12 87 123
Cuts at: 2826 2954 3669 4083 4331 4366 760
Size: 128 715 414 248 35 3047
Fragments arranged by size:
3047 715 585 505 414 348 323 248
128 123 87 83 35 12
BanII G_rGCy'C
Cuts at: 773 987 1044 1057 1106 1262 1561 2001 2728
Size: 214 57 13 49 156 299 440 727
Cuts at: 2728 3256 3271 3298 3379 3667 4697 773
Size: 528 15 27 81 288 1030 2729
Fragments arranged by size:
2729 1030 727 528 440 299 288 214
156 81 57 49 27 15 13
BbeI G_GCGC'C
Cuts at: 4087 4335 4087
Size: 248 6405
BbsI GAAGACnn'nnnn_
Cuts at: 952 2557 952
Size: 1605 5048
BbvCI CC'TCA_GC
Cuts at: 1835 2021 2479 1835
Size: 186 458 6009
Fragments arranged by size:
6009 458 186
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 900 934 3421 3455 3593 3627 900
Size: 34 2487 34 138 34 3926
Fragments arranged by size:
3926 2487 138 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1262 4551 5607 6653 1262
Size: 3289 1056 1046 1262
Fragments arranged by size:
3289 1262 1056 1046
BclI T'GATC_A
Cuts at: 1109 2897 4173 1109
Size: 1788 1276 3589
Fragments arranged by size:
3589 1788 1276
BfrBI ATG'CAT
Cuts at: 2256 3633 2256
Size: 1377 5276
BglI GCCn_nnn'nGGC
Cuts at: 2474 2835 2474
Size: 361 6292
BglII A'GATC_T
Cuts at: 4168 6205 4168
Size: 2037 4616
BlpI GC'TnA_GC
Cuts at: 1898 3765 1898
Size: 1867 4786
Bme1580I G_kGCm'C
Cuts at: 966 1057 1106 1262 2679 2708 2728 3370 3379
Size: 91 49 156 1417 29 20 642 9
Cuts at: 3379 4278 4371 5495 966
Size: 899 93 1124 2124
Fragments arranged by size:
2124 1417 1124 899 642 156 93 91
49 29 20 9
BmrI ACTGGGnnnn_n'
Cuts at: 189 224 678 717 850 1204 1555 1620 1886
Size: 35 454 39 133 354 351 65 266
Cuts at: 1886 3865 3946 4281 6425 189
Size: 1979 81 335 2144 417
Fragments arranged by size:
2144 1979 454 417 354 351 335 266
133 81 65 39 35
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 954 5020 954
Size: 4066 2587
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 197 468 534 1111 1320 3240 3282 3498 197
Size: 271 66 577 209 1920 42 216 3352
Fragments arranged by size:
3352 1920 577 271 216 209 66 42
Bpu10I CC'TnA_GC
Cuts at: 755 1835 2021 2479 3250 755
Size: 1080 186 458 771 4158
Fragments arranged by size:
4158 1080 771 458 186
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 1636 3829 4708 5422 5720 268
Size: 1368 2193 879 714 298 1201
Fragments arranged by size:
2193 1368 1201 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 673 1023 1063 1149 2142 6357 673
Size: 350 40 86 993 4215 969
Fragments arranged by size:
4215 993 969 350 86 40
BsaAI yAC'GTr
Cuts at: 2046 2369 2541 2674 3116 4006 2046
Size: 323 172 133 442 890 4693
Fragments arranged by size:
4693 890 442 323 172 133
BsaBI GATnn'nnATC
Cuts at: 6 2884 2902 4167 6204 6
Size: 2878 18 1265 2037 455
Fragments arranged by size:
2878 2037 1265 455 18
BsaHI Gr'CG_yC
Cuts at: 331 2915 4084 4332 5034 6530 331
Size: 2584 1169 248 702 1496 454
Fragments arranged by size:
2584 1496 1169 702 454 248
BsaWI w'CCGG_w
Cuts at: 136 633 987 3842 4363 5041 5452 5599 5873
Size: 497 354 2855 521 678 411 147 274
Cuts at: 5873 136
Size: 916
Fragments arranged by size:
2855 916 678 521 497 411 354 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 670 700 2401 2431 670
Size: 30 1701 30 4892
Fragments arranged by size:
4892 1701 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 598 1569 2890 598
Size: 971 1321 4361
Fragments arranged by size:
4361 1321 971
BseYI C'CCAG_C
Cuts at: 269 747 1239 1494 1620 1993 2379 2519 2678
Size: 478 492 255 126 373 386 140 159
Cuts at: 2678 3126 3274 4078 4836 5501 5909 269
Size: 448 148 804 758 665 408 1013
Fragments arranged by size:
1013 804 758 665 492 478 448 408
386 373 255 159 148 140 126
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 443 799 1262 3370 443
Size: 356 463 2108 3726
Fragments arranged by size:
3726 2108 463 356
BsiEI CG_ry'CG
Cuts at: 104 162 4195 4241 5471 104
Size: 58 4033 46 1230 1286
Fragments arranged by size:
4033 1286 1230 58 46
BsiHKAI G_wGCw'C
Cuts at: 2429 3256 3667 4445 4635 5495 2429
Size: 827 411 778 190 860 3587
Fragments arranged by size:
3587 860 827 778 411 190
BsmI GAATG_Cn'
Cuts at: 2256 2256
Size: 6653
Bsp1286I G_dGCh'C
Cuts at: 773 966 987 1044 1057 1106 1262 1561 2001
Size: 193 21 57 13 49 156 299 440
Cuts at: 2001 2429 2679 2708 2728 3256 3271 3298 3370
Size: 428 250 29 20 528 15 27 72
Cuts at: 3370 3379 3667 4278 4371 4445 4635 4697 5495
Size: 9 288 611 93 74 190 62 798
Cuts at: 5495 773
Size: 1931
Fragments arranged by size:
1931 798 611 528 440 428 299 288
250 193 190 156 93 74 72 62
57 49 29 27 21 20 15 13
9
BspEI T'CCGG_A
Cuts at: 633 987 633
Size: 354 6299
BspHI T'CATG_A
Cuts at: 1418 5085 1418
Size: 3667 2986
BspMI ACCTGCnnnn'nnnn_
Cuts at: 606 922 3703 3917 4219 4600 606
Size: 316 2781 214 302 381 2659
Fragments arranged by size:
2781 2659 381 316 302 214
BsrBI CCG'CTC
Cuts at: 50 223 4945 4999 6483 6648 50
Size: 173 4722 54 1484 165 55
Fragments arranged by size:
4722 1484 173 165 55 54
BsrDI GCAATG_nn'
Cuts at: 156 1507 4565 156
Size: 1351 3058 2244
Fragments arranged by size:
3058 2244 1351
BsrFI r'CCGG_y
Cuts at: 157 2623 4651 4832 157
Size: 2466 2028 181 1978
Fragments arranged by size:
2466 2028 1978 181
BssSI C'ACGA_G
Cuts at: 178 274 4924 5632 178
Size: 96 4650 708 1199
Fragments arranged by size:
4650 1199 708 96
BstAPI GCAn_nnn'nTGC
Cuts at: 3239 4030 3239
Size: 791 5862
BstBI TT'CG_AA
Cuts at: 5014 5014
Size: 6653
BstEII G'GTnAC_C
Cuts at: 834 1485 1569 834
Size: 651 84 5918
Fragments arranged by size:
5918 651 84
BstXI CCAn_nnnn'nTGG
Cuts at: 237 3345 237
Size: 3108 3545
BstYI r'GATC_y
Cuts at: 1 1313 2879 2942 3491 3678 4145 4168 4503
Size: 1312 1566 63 549 187 467 23 335
Cuts at: 4503 4749 5055 5067 5153 6205 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1566 1312 1052 549 467 449 335 306
246 187 86 63 23 12
Bsu36I CC'TnA_GG
Cuts at: 1394 1737 1394
Size: 343 6310
BtgI C'CryG_G
Cuts at: 1351 2532 2729 2919 2966 1351
Size: 1181 197 190 47 5038
Fragments arranged by size:
5038 1181 197 190 47
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1468 3184 3424 4603 4784 4947 1468
Size: 1716 240 1179 181 163 3174
Fragments arranged by size:
3174 1716 1179 240 181 163
BtsI GCAGTG_nn'
Cuts at: 1988 2900 3392 3896 1988
Size: 912 492 504 4745
Fragments arranged by size:
4745 912 504 492
DraI TTT'AAA
Cuts at: 3652 3652
Size: 6653
DraIII CAC_nnn'GTG
Cuts at: 197 197
Size: 6653
DrdI GACnn_nn'nnGTC
Cuts at: 706 999 2068 3045 4359 5703 706
Size: 293 1069 977 1314 1344 1656
Fragments arranged by size:
1656 1344 1314 1069 977 293
EaeI y'GGCC_r
Cuts at: 159 494 1640 2156 2273 2625 2694 2751 3455
Size: 335 1146 516 117 352 69 57 704
Cuts at: 3455 3614 4238 4412 4803 4830 6175 159
Size: 159 624 174 391 27 1345 637
Fragments arranged by size:
1345 1146 704 637 624 516 391 352
335 174 159 117 69 57 27
EagI C'GGCC_G
Cuts at: 159 4238 159
Size: 4079 2574
EarI CTCTTCn'nnn_
Cuts at: 632 1417 4676 4886 632
Size: 785 3259 210 2399
Fragments arranged by size:
3259 2399 785 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 595 3021 3390 3462 3484 4861 5603 5749 6463
Size: 2426 369 72 22 1377 742 146 714
Cuts at: 6463 595
Size: 785
Fragments arranged by size:
2426 1377 785 742 714 369 146 72
22
EcoICRI GAG'CTC
Cuts at: 3254 3665 3254
Size: 411 6242
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 197 468 534 1111 1320 3240 3282 3498 3595
Size: 271 66 577 209 1920 42 216 97
Cuts at: 3595 4478 4910 5263 5925 197
Size: 883 432 353 662 925
Fragments arranged by size:
1920 925 883 662 577 432 353 271
216 209 97 66 42
EcoNI CCTnn'n_nnAGG
Cuts at: 317 1432 317
Size: 1115 5538
EcoO109I rG'GnC_Cy
Cuts at: 279 784 1053 1054 1102 1258 1447 1881 2725
Size: 505 269 1 48 156 189 434 844
Cuts at: 2725 3375 3376 3792 279
Size: 650 1 416 3140
Fragments arranged by size:
3140 844 650 505 434 416 269 189
156 48 1 1
EcoRI G'AATT_C
Cuts at: 3657 3657
Size: 6653
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4672 4672
Size: 6653
FspI TGC'GCA
Cuts at: 4434 4434
Size: 6653
HaeII r_GCGC'y
Cuts at: 259 525 2308 2469 2486 3592 4087 4335 5565
Size: 266 1783 161 17 1106 495 248 1230
Cuts at: 5565 6336 259
Size: 771 576
Fragments arranged by size:
1783 1230 1106 771 576 495 266 248
161 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1647 1679 4495 4525 4527 4557 1647
Size: 32 2816 30 2 30 3743
Fragments arranged by size:
3743 2816 32 30 30 2
HincII GTy'rAC
Cuts at: 3692 3692
Size: 6653
KasI G'GCGC_C
Cuts at: 4083 4331 4083
Size: 248 6405
KpnI G_GTAC'C
Cuts at: 764 3673 764
Size: 2909 3744
MluI A'CGCG_T
Cuts at: 6086 6086
Size: 6653
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1699 2474 5412 5596 1699
Size: 775 2938 184 2756
Fragments arranged by size:
2938 2756 775 184
MscI TGG'CCA
Cuts at: 1642 2275 2753 4414 1642
Size: 633 478 1661 3881
Fragments arranged by size:
3881 1661 633 478
MslI CAynn'nnrTG
Cuts at: 419 667 2048 3118 3320 3343 4642 4769 419
Size: 248 1381 1070 202 23 1299 127 2303
Fragments arranged by size:
2303 1381 1299 1070 248 202 127 23
MspA1I CmG'CkG
Cuts at: 1897 2091 2166 2731 3411 3764 4078 4438 5222
Size: 194 75 565 680 353 314 360 784
Cuts at: 5222 5467 1897
Size: 245 3083
Fragments arranged by size:
3083 784 680 565 360 353 314 245
194 75
NaeI GCC'GGC
Cuts at: 159 4834 159
Size: 4675 1978
NarI GG'CG_CC
Cuts at: 4084 4332 4084
Size: 248 6405
NcoI C'CATG_G
Cuts at: 2966 2966
Size: 6653
NgoMIV G'CCGG_C
Cuts at: 157 4832 157
Size: 4675 1978
NsiI A_TGCA'T
Cuts at: 2258 3635 2258
Size: 1377 5276
NspI r_CATG'y
Cuts at: 1292 1364 1520 2639 3633 3706 4206 5809 1292
Size: 72 156 1119 994 73 500 1603 2136
Fragments arranged by size:
2136 1603 1119 994 500 156 73 72
PasI CC'CwG_GG
Cuts at: 1050 1886 2492 1050
Size: 836 606 5211
Fragments arranged by size:
5211 836 606
PciI A'CATG_T
Cuts at: 1516 5805 1516
Size: 4289 2364
PflMI CCAn_nnn'nTGG
Cuts at: 489 489
Size: 6653
PfoI T'CCnGG_A
Cuts at: 236 506 1869 2005 3167 3256 236
Size: 270 1363 136 1162 89 3633
Fragments arranged by size:
3633 1363 1162 270 136 89
PmeI GTTT'AAAC
Cuts at: 3652 3652
Size: 6653
PmlI CAC'GTG
Cuts at: 2046 2369 2541 2674 3116 2046
Size: 323 172 133 442 5583
Fragments arranged by size:
5583 442 323 172 133
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 670 702 5099 5131 670
Size: 32 4397 32 2192
Fragments arranged by size:
4397 2192 32 32
PpuMI rG'GwC_Cy
Cuts at: 784 1881 784
Size: 1097 5556
PshAI GACnn'nnGTC
Cuts at: 723 2240 723
Size: 1517 5136
PspOMI G'GGCC_C
Cuts at: 1053 1102 1258 2724 3375 1053
Size: 49 156 1466 651 4331
Fragments arranged by size:
4331 1466 651 156 49
PstI C_TGCA'G
Cuts at: 718 743 1896 2874 3028 3700 4385 718
Size: 25 1153 978 154 672 685 2986
Fragments arranged by size:
2986 1153 978 685 672 154 25
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6653
PvuII CAG'CTG
Cuts at: 1897 2091 3411 4078 4438 1897
Size: 194 1320 667 360 4112
Fragments arranged by size:
4112 1320 667 360 194
RsrII CG'GwC_CG
Cuts at: 2840 4848 2840
Size: 2008 4645
SacI G_AGCT'C
Cuts at: 3256 3667 3256
Size: 411 6242
SacII CC_GC'GG
Cuts at: 2732 2732
Size: 6653
SalI G'TCGA_C
Cuts at: 3690 3690
Size: 6653
SanDI GG'GwC_CC
Cuts at: 1881 1881
Size: 6653
SapI GCTCTTCn'nnn_
Cuts at: 1417 4676 4886 1417
Size: 3259 210 3184
Fragments arranged by size:
3259 3184 210
SbfI CC_TGCA'GG
Cuts at: 3700 3700
Size: 6653
ScaI AGT'ACT
Cuts at: 6170 6170
Size: 6653
SfcI C'TryA_G
Cuts at: 33 170 714 739 1892 2057 2870 3024 3063
Size: 137 544 25 1153 165 813 154 39
Cuts at: 3063 3696 4381 5349 5540 33
Size: 633 685 968 191 1146
Fragments arranged by size:
1153 1146 968 813 685 633 544 191
165 154 137 39 25
SfoI GGC'GCC
Cuts at: 4085 4333 4085
Size: 248 6405
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6653
SmaI CCC'GGG
Cuts at: 1445 2083 2341 3381 3675 1445
Size: 638 258 1040 294 4423
Fragments arranged by size:
4423 1040 638 294 258
SmlI C'TyrA_G
Cuts at: 283 1651 3808 4723 5437 5699 283
Size: 1368 2157 915 714 262 1237
Fragments arranged by size:
2157 1368 1237 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6653
SphI G_CATG'C
Cuts at: 3706 4206 3706
Size: 500 6153
StuI AGG'CCT
Cuts at: 376 514 2176 376
Size: 138 1662 4853
Fragments arranged by size:
4853 1662 138
StyI C'CwwG_G
Cuts at: 290 497 1763 1769 2171 2441 2966 3787 4141
Size: 207 1266 6 402 270 525 821 354
Cuts at: 4141 290
Size: 2802
Fragments arranged by size:
2802 1266 821 525 402 354 270 207
6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 208 1212 1776 1951 2428 2634 3108 3170 4236
Size: 1004 564 175 477 206 474 62 1066
Cuts at: 4236 4369 4828 5037 208
Size: 133 459 209 1824
Fragments arranged by size:
1824 1066 1004 564 477 474 459 209
206 175 133 62
TatI w'GTAC_w
Cuts at: 6168 6168
Size: 6653
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6170 6202 6170
Size: 32 6621
Tth111I GACn'n_nGTC
Cuts at: 330 2334 4450 330
Size: 2004 2116 2533
Fragments arranged by size:
2533 2116 2004
XbaI T'CTAG_A
Cuts at: 3684 3684
Size: 6653
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 558 1980 2470 4148 558
Size: 1422 490 1678 3063
Fragments arranged by size:
3063 1678 1422 490
XmaI C'CCGG_G
Cuts at: 1443 2081 2339 3379 3673 1443
Size: 638 258 1040 294 4423
Fragments arranged by size:
4423 1040 638 294 258
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AscI AvrII BaeI BmgBI
BmtI BsiWI BsmBI BsrGI BssHII BstZ17I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI PacI PsiI PspXI PsrI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI