Restriction map for pF1KB8403
 (Linear) (Six-base) MAPSORT of: KIBB8403.seq  Check: 401  from: 1  to: 2145



KIBB8403.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:05  ..

AatII G_ACGT'C

Cuts at:      0     503    2145 
   Size:        503    1642
 
AccI GT'mk_AC

Cuts at:      0     470    2145 
   Size:        470    1675
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     959    1517    2145 
   Size:        959     558     628

  Fragments arranged by size: 

                 959     628     558
 
AflIII A'CryG_T

Cuts at:      0      93    1098    1766    2145 
   Size:         93    1005     668     379

  Fragments arranged by size: 

                1005     668     379      93
 
AlwNI CAG_nnn'CTG

Cuts at:      0     426    2014    2145 
   Size:        426    1588     131

  Fragments arranged by size: 

                1588     426     131
 
ApoI r'AATT_y

Cuts at:      0     343     381     800     821    1446    1474    1576    1866 
   Size:        343      38     419      21     625      28     102     290

Cuts at:   1866    2145 
   Size:        279

  Fragments arranged by size: 

                 625     419     343     290     279     102      38      28
                  21
 
AseI AT'TA_AT

Cuts at:      0    1030    1469    2145 
   Size:       1030     439     676

  Fragments arranged by size: 

                1030     676     439
 
BanII G_rGCy'C

Cuts at:      0     578    2145 
   Size:        578    1567
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     296    2145 
   Size:        296    1849
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      14      48    1048    1082    2145 
   Size:         14      34    1000      34    1063

  Fragments arranged by size: 

                1063    1000      34      34      14
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     850    1716    2145 
   Size:        850     866     429

  Fragments arranged by size: 

                 866     850     429
 
BclI T'GATC_A

Cuts at:      0      48    2145 
   Size:         48    2097
 
BlpI GC'TnA_GC

Cuts at:      0     833    2145 
   Size:        833    1312
 
Bme1580I G_kGCm'C

Cuts at:      0    1087    2145 
   Size:       1087    1058
 
BmgBI CAC'GTC

Cuts at:      0      15    2145 
   Size:         15    2130
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     766    2145 
   Size:        766    1379
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     885    2145 
   Size:        885    1260
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     611    1914    2145 
   Size:        611    1303     231

  Fragments arranged by size: 

                1303     611     231
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1832    2039    2145 
   Size:       1832     207     106

  Fragments arranged by size: 

                1832     207     106
 
BsaHI Gr'CG_yC

Cuts at:      0     500    2145 
   Size:        500    1645
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1637    2145 
   Size:       1637     508
 
Bsp1286I G_dGCh'C

Cuts at:      0     578    1087    2145 
   Size:        578     509    1058

  Fragments arranged by size: 

                1058     578     509
 
BspHI T'CATG_A

Cuts at:      0      45    1980    2145 
   Size:         45    1935     165

  Fragments arranged by size: 

                1935     165      45
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     202     500    2145 
   Size:        202     298    1645

  Fragments arranged by size: 

                1645     298     202
 
BsrGI T'GTAC_A

Cuts at:      0     931    2145 
   Size:        931    1214
 
BstBI TT'CG_AA

Cuts at:      0    1173    2145 
   Size:       1173     972
 
BstEII G'GTnAC_C

Cuts at:      0     189    2145 
   Size:        189    1956
 
BstYI r'GATC_y

Cuts at:      0      99     390     526    2145 
   Size:         99     291     136    1619

  Fragments arranged by size: 

                1619     291     136      99
 
BstZ17I GTA'TAC

Cuts at:      0     471    2145 
   Size:        471    1674
 
DraI TTT'AAA

Cuts at:      0     639     924     986    1329    2145 
   Size:        639     285      62     343     816

  Fragments arranged by size: 

                 816     639     343     285      62
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1513    2145 
   Size:       1513     632
 
EarI CTCTTCn'nnn_

Cuts at:      0     406    2104    2145 
   Size:        406    1698      41

  Fragments arranged by size: 

                1698     406      41
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      25    2145 
   Size:         25    2120
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     611     959    1517    1914    2145 
   Size:        611     348     558     397     231

  Fragments arranged by size: 

                 611     558     397     348     231
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1834    2145 
   Size:       1834     311
 
EcoRI G'AATT_C

Cuts at:      0    1474    2145 
   Size:       1474     671
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     988    1570    2145 
   Size:        988     582     575

  Fragments arranged by size: 

                 988     582     575
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     123     155    1019    1051    1672    1704    2145 
   Size:        123      32     864      32     621      32     441

  Fragments arranged by size: 

                 864     621     441     123      32      32      32
 
HincII GTy'rAC

Cuts at:      0     679    1506    2145 
   Size:        679     827     639

  Fragments arranged by size: 

                 827     679     639
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     504    2145 
   Size:        504    1641
 
MslI CAynn'nnrTG

Cuts at:      0    1345    2145 
   Size:       1345     800
 
MspA1I CmG'CkG

Cuts at:      0     426    2145 
   Size:        426    1719
 
NdeI CA'TA_TG

Cuts at:      0    1346    2145 
   Size:       1346     799
 
NspI r_CATG'y

Cuts at:      0      97    1102    1615    1770    2145 
   Size:         97    1005     513     155     375

  Fragments arranged by size: 

                1005     513     375     155      97
 
PciI A'CATG_T

Cuts at:      0      93    1098    1766    2145 
   Size:         93    1005     668     379

  Fragments arranged by size: 

                1005     668     379      93
 
PpuMI rG'GwC_Cy

Cuts at:      0    1834    2145 
   Size:       1834     311
 
PsiI TTA'TAA

Cuts at:      0     320    2145 
   Size:        320    1825
 
PvuII CAG'CTG

Cuts at:      0     426    2145 
   Size:        426    1719
 
SexAI A'CCwGG_T

Cuts at:      0     717    2145 
   Size:        717    1428
 
SfcI C'TryA_G

Cuts at:      0     303     418    1943    2145 
   Size:        303     115    1525     202

  Fragments arranged by size: 

                1525     303     202     115
 
SmlI C'TyrA_G

Cuts at:      0    1847    2054    2145 
   Size:       1847     207      91

  Fragments arranged by size: 

                1847     207      91
 
SspI AAT'ATT

Cuts at:      0     181    2145 
   Size:        181    1964
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     810    2145 
   Size:        810    1335
 
TatI w'GTAC_w

Cuts at:      0      90     931    1411    2082    2145 
   Size:         90     841     480     671      63

  Fragments arranged by size: 

                 841     671     480      90      63
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1578    1610    2145 
   Size:       1578      32     535

  Fragments arranged by size: 

                1578     535      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     456    2145 
   Size:        456    1689
 
XbaI T'CTAG_A

Cuts at:      0    1618    2145 
   Size:       1618     527
 
XmnI GAAnn'nnTTC

Cuts at:      0    1355    1577    2145 
   Size:       1355     222     568

  Fragments arranged by size: 

                1355     568     222
 
ZraI GAC'GTC

Cuts at:      0     501    2145 
   Size:        501    1644
 

 Enzymes that do cut:

    AatII     AccI     AcuI   AflIII    AlwNI     ApoI     AseI    BanII
     BbsI     BcgI    BciVI     BclI     BlpI Bme1580I    BmgBI     BmrI
     BplI     BpmI    BpuEI    BsaHI    BseRI Bsp1286I    BspHI    BspMI
    BsrGI    BstBI   BstEII    BstYI  BstZ17I     DraI     DrdI     EarI
     EciI  Eco57MI EcoO109I    EcoRI     FalI    Hin4I   HincII     MmeI
     MslI   MspA1I     NdeI     NspI     PciI    PpuMI     PsiI    PvuII
    SexAI     SfcI     SmlI     SspI    TaqII     TatI     TstI  Tth111I
     XbaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AleI
     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BanI     BbeI    BbvCI    BfrBI     BglI    BglII
     BmtI   Bpu10I     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BsrBI
    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstXI   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI   DraIII     EaeI     EagI  EcoICRI
    EcoNI    EcoRV     FseI     FspI    FspAI    HaeII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     NaeI     NarI     NcoI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI
     PfoI     PmeI     PmlI     PpiI    PshAI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     StuI     StyI     SwaI     XcmI     XhoI
     XmaI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com