Restriction map for pF1KB8376
 (Linear) (Six-base) MAPSORT of: KIBB8376.seq  Check: 8308  from: 1  to: 1878



KIBB8376.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:05  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     582    1878 
   Size:        582    1296
 
AatII G_ACGT'C

Cuts at:      0     707    1878 
   Size:        707    1171
 
AleI CACnn'nnGTG

Cuts at:      0      65     460     586    1878 
   Size:         65     395     126    1292

  Fragments arranged by size: 

                1292     395     126      65
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     322     604    1878 
   Size:        322     282    1274

  Fragments arranged by size: 

                1274     322     282
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1095    1290    1878 
   Size:       1095     195     588

  Fragments arranged by size: 

                1095     588     195
 
AvaI C'yCGr_G

Cuts at:      0      50     325    1150    1271    1878 
   Size:         50     275     825     121     607

  Fragments arranged by size: 

                 825     607     275     121      50
 
BanI G'GyrC_C

Cuts at:      0      67     432     480     580    1483    1552    1698    1724 
   Size:         67     365      48     100     903      69     146      26

Cuts at:   1724    1878 
   Size:        154

  Fragments arranged by size: 

                 903     365     154     146     100      69      67      48
                  26
 
BanII G_rGCy'C

Cuts at:      0     548     763     959    1518    1636    1769    1878 
   Size:        548     215     196     559     118     133     109

  Fragments arranged by size: 

                 559     548     215     196     133     118     109
 
BbeI G_GCGC'C

Cuts at:      0    1487    1556    1728    1878 
   Size:       1487      69     172     150

  Fragments arranged by size: 

                1487     172     150      69
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     210     902    1878 
   Size:        210     692     976

  Fragments arranged by size: 

                 976     692     210
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1383    1417    1878 
   Size:       1383      34     461

  Fragments arranged by size: 

                1383     461      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     916    1878 
   Size:        916     962
 
BlpI GC'TnA_GC

Cuts at:      0     310     514    1878 
   Size:        310     204    1364

  Fragments arranged by size: 

                1364     310     204
 
Bme1580I G_kGCm'C

Cuts at:      0     437     583     617    1701    1878 
   Size:        437     146      34    1084     177

  Fragments arranged by size: 

                1084     437     177     146      34
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      72    1878 
   Size:         72    1806
 
BmtI G_CTAG'C

Cuts at:      0    1852    1878 
   Size:       1852      26
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     753    1044    1808    1878 
   Size:        753     291     764      70

  Fragments arranged by size: 

                 764     753     291      70
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     889     895    1150    1270    1516    1878 
   Size:        889       6     255     120     246     362

  Fragments arranged by size: 

                 889     362     255     246     120       6
 
Bpu10I CC'TnA_GC

Cuts at:      0     509    1783    1878 
   Size:        509    1274      95

  Fragments arranged by size: 

                1274     509      95
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1032    1878 
   Size:       1032     846
 
BsaAI yAC'GTr

Cuts at:      0     107     768    1878 
   Size:        107     661    1110

  Fragments arranged by size: 

                1110     661     107
 
BsaBI GATnn'nnATC

Cuts at:      0    1582    1878 
   Size:       1582     296
 
BsaHI Gr'CG_yC

Cuts at:      0     704     902    1484    1553    1725    1878 
   Size:        704     198     582      69     172     153

  Fragments arranged by size: 

                 704     582     198     172     153      69
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1366    1396    1878 
   Size:       1366      30     482

  Fragments arranged by size: 

                1366     482      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     729     892    1234    1755    1858    1878 
   Size:        729     163     342     521     103      20

  Fragments arranged by size: 

                 729     521     342     163     103      20
 
BseYI C'CCAG_C

Cuts at:      0     330     522     537     555     642    1636    1749    1878 
   Size:        330     192      15      18      87     994     113     129

  Fragments arranged by size: 

                 994     330     192     129     113      87      18      15
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     282     589     610    1303    1446    1878 
   Size:        282     307      21     693     143     432

  Fragments arranged by size: 

                 693     432     307     282     143      21
 
BsiEI CG_ry'CG

Cuts at:      0    1142    1878 
   Size:       1142     736
 
BsiHKAI G_wGCw'C

Cuts at:      0     395    1042    1244    1409    1878 
   Size:        395     647     202     165     469

  Fragments arranged by size: 

                 647     469     395     202     165
 
BsmI GAATG_Cn'

Cuts at:      0     930    1878 
   Size:        930     948
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     117    1015    1878 
   Size:        117     898     863

  Fragments arranged by size: 

                 898     863     117
 
Bsp1286I G_dGCh'C

Cuts at:      0     395     437     548     583     617     763     959    1042 
   Size:        395      42     111      35      34     146     196      83

Cuts at:   1042    1244    1409    1518    1636    1701    1769    1878 
   Size:        202     165     109     118      65      68     109

  Fragments arranged by size: 

                 395     202     196     165     146     118     111     109
                 109      83      68      65      42      35      34
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     582    1878 
   Size:        582    1296
 
BsrBI CCG'CTC

Cuts at:      0    1371    1878 
   Size:       1371     507
 
BsrDI GCAATG_nn'

Cuts at:      0    1353    1878 
   Size:       1353     525
 
BsrFI r'CCGG_y

Cuts at:      0     560    1137    1878 
   Size:        560     577     741

  Fragments arranged by size: 

                 741     577     560
 
BssSI C'ACGA_G

Cuts at:      0     244     529     618    1402    1878 
   Size:        244     285      89     784     476

  Fragments arranged by size: 

                 784     476     285     244      89
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     612    1878 
   Size:        612    1266
 
BstBI TT'CG_AA

Cuts at:      0      98    1878 
   Size:         98    1780
 
BstEII G'GTnAC_C

Cuts at:      0     882    1878 
   Size:        882     996
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     429    1494    1878 
   Size:        429    1065     384

  Fragments arranged by size: 

                1065     429     384
 
BstYI r'GATC_y

Cuts at:      0    1091    1732    1878 
   Size:       1091     641     146

  Fragments arranged by size: 

                1091     641     146
 
BtgI C'CryG_G

Cuts at:      0     351     950    1100    1322    1556    1878 
   Size:        351     599     150     222     234     322

  Fragments arranged by size: 

                 599     351     322     234     222     150
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1325    1878 
   Size:       1325     553
 
BtsI GCAGTG_nn'

Cuts at:      0      16     598    1257    1291    1878 
   Size:         16     582     659      34     587

  Fragments arranged by size: 

                 659     587     582      34      16
 
DraIII CAC_nnn'GTG

Cuts at:      0     612    1878 
   Size:        612    1266
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     832    1878 
   Size:        832    1046
 
EaeI y'GGCC_r

Cuts at:      0     633     851     917     933    1007    1139    1194    1878 
   Size:        633     218      66      16      74     132      55     684

  Fragments arranged by size: 

                 684     633     218     132      74      66      55      16
            
 
EagI C'GGCC_G

Cuts at:      0    1139    1878 
   Size:       1139     739
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     889     895    1150    1270    1516    1878 
   Size:        889       6     255     120     246     362

  Fragments arranged by size: 

                 889     362     255     246     120       6
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     841    1878 
   Size:        841    1037
 
EcoO109I rG'GnC_Cy

Cuts at:      0     279     357    1443    1878 
   Size:        279      78    1086     435

  Fragments arranged by size: 

                1086     435     279      78
 
EcoRV GAT'ATC

Cuts at:      0     408    1878 
   Size:        408    1470
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     363    1878 
   Size:        363    1515
 
HaeII r_GCGC'y

Cuts at:      0    1487    1556    1728    1878 
   Size:       1487      69     172     150

  Fragments arranged by size: 

                1487     172     150      69
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1324    1356    1878 
   Size:       1324      32     522

  Fragments arranged by size: 

                1324     522      32
 
HincII GTy'rAC

Cuts at:      0     414    1878 
   Size:        414    1464
 
KasI G'GCGC_C

Cuts at:      0    1483    1552    1724    1878 
   Size:       1483      69     172     154

  Fragments arranged by size: 

                1483     172     154      69
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1331    1878 
   Size:       1331     547
 
MscI TGG'CCA

Cuts at:      0     935    1196    1878 
   Size:        935     261     682

  Fragments arranged by size: 

                 935     682     261
 
MslI CAynn'nnrTG

Cuts at:      0      65     430     460     586    1878 
   Size:         65     365      30     126    1292

  Fragments arranged by size: 

                1292     365     126      65      30
 
MspA1I CmG'CkG

Cuts at:      0     157     473     550     807    1128    1397    1878 
   Size:        157     316      77     257     321     269     481

  Fragments arranged by size: 

                 481     321     316     269     257     157      77
 
NaeI GCC'GGC

Cuts at:      0     562    1139    1878 
   Size:        562     577     739

  Fragments arranged by size: 

                 739     577     562
 
NarI GG'CG_CC

Cuts at:      0    1484    1553    1725    1878 
   Size:       1484      69     172     153

  Fragments arranged by size: 

                1484     172     153      69
 
NgoMIV G'CCGG_C

Cuts at:      0     560    1137    1878 
   Size:        560     577     741

  Fragments arranged by size: 

                 741     577     560
 
NheI G'CTAG_C

Cuts at:      0    1848    1878 
   Size:       1848      30
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     642    1878 
   Size:        642    1236
 
PfoI T'CCnGG_A

Cuts at:      0     137     401     839     866    1247    1878 
   Size:        137     264     438      27     381     631

  Fragments arranged by size: 

                 631     438     381     264     137      27
 
PmlI CAC'GTG

Cuts at:      0     107     768    1878 
   Size:        107     661    1110

  Fragments arranged by size: 

                1110     661     107
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1360    1392    1878 
   Size:       1360      32     486

  Fragments arranged by size: 

                1360     486      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     279     357    1878 
   Size:        279      78    1521

  Fragments arranged by size: 

                1521     279      78
 
PstI C_TGCA'G

Cuts at:      0     680    1127    1878 
   Size:        680     447     751

  Fragments arranged by size: 

                 751     680     447
 
PvuII CAG'CTG

Cuts at:      0     473    1128    1878 
   Size:        473     655     750

  Fragments arranged by size: 

                 750     655     473
 
ScaI AGT'ACT

Cuts at:      0     343    1168    1878 
   Size:        343     825     710

  Fragments arranged by size: 

                 825     710     343
 
SfcI C'TryA_G

Cuts at:      0     676    1123    1878 
   Size:        676     447     755

  Fragments arranged by size: 

                 755     676     447
 
SfoI GGC'GCC

Cuts at:      0    1485    1554    1726    1878 
   Size:       1485      69     172     152

  Fragments arranged by size: 

                1485     172     152      69
 
StuI AGG'CCT

Cuts at:      0     655    1878 
   Size:        655    1223
 
StyI C'CwwG_G

Cuts at:      0    1820    1878 
   Size:       1820      58
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     247     576    1878 
   Size:        247     329    1302

  Fragments arranged by size: 

                1302     329     247
 
TatI w'GTAC_w

Cuts at:      0     341     377    1166    1382    1878 
   Size:        341      36     789     216     496

  Fragments arranged by size: 

                 789     496     341     216      36
 
Tth111I GACn'n_nGTC

Cuts at:      0     901    1878 
   Size:        901     977
 
ZraI GAC'GTC

Cuts at:      0     705    1878 
   Size:        705    1173
 

 Enzymes that do cut:

     AarI    AatII     AleI     AlfI    AlwNI     AvaI     BanI    BanII
     BbeI     BbsI     BcgI     BglI     BlpI Bme1580I     BmrI     BmtI
     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI     BtgI    BtgZI     BtsI   DraIII     DrdI     EaeI
     EagI  Eco57MI    EcoNI EcoO109I    EcoRV     FalI    HaeII    Hin4I
   HincII     KasI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
   NgoMIV     NheI    PflMI     PfoI     PmlI     PpiI    PpuMI     PstI
    PvuII     ScaI     SfcI     SfoI     StuI     StyI    TaqII     TatI
  Tth111I     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI    BbvCI    BciVI     BclI    BfrBI    BglII
    BmgBI    BpuEI    BsaWI    BsiWI    BspEI    BspHI    BsrGI   BssHII
  BstZ17I   Bsu36I     ClaI    CspCI     DraI     EarI     EciI  EcoICRI
    EcoRI     FseI     FspI    FspAI  HindIII     HpaI     KpnI     MfeI
     MluI     NcoI     NdeI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI     PmeI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
    SexAI     SfiI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI     TstI     XbaI     XcmI     XhoI
     XmaI     XmnI
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