Restriction map for pF1KB8367
 (Circular) (Six-base) MAPSORT of: pF1KB8367.seq  Check: 666  from: 1  to: 4007



pF1KB8367  4007 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:28  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    767     767 
   Size:       4007
 
AccI GT'mk_AC

Cuts at:    326    1045     326 
   Size:        719    3288
 
Acc65I G'GTAC_C

Cuts at:   1023    1023 
   Size:       4007
 
AclI AA'CG_TT

Cuts at:   3847    3847 
   Size:       4007
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1832    2264    3011    3279    1832 
   Size:        432     747     268    2560

  Fragments arranged by size: 

                2560     747     432     268
 
AflIII A'CryG_T

Cuts at:   1359    2463    3440    1359 
   Size:       1104     977    1926

  Fragments arranged by size: 

                1926    1104     977
 
AgeI A'CCGG_T

Cuts at:   2395    2395 
   Size:       4007
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    646     646 
   Size:       4007
 
AlwNI CAG_nnn'CTG

Cuts at:    598    1312    2879     598 
   Size:        714    1567    1726

  Fragments arranged by size: 

                1726    1567     714
 
ApaLI G'TGCA_C

Cuts at:   2777    2777 
   Size:       4007
 
ApoI r'AATT_y

Cuts at:   1011    3360    3616    1011 
   Size:       2349     256    1402

  Fragments arranged by size: 

                2349    1402     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4007
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4007
 
AvaI C'yCGr_G

Cuts at:   1027    1027 
   Size:       4007
 
BamHI G'GATC_C

Cuts at:   1032    1032 
   Size:       4007
 
BanI G'GyrC_C

Cuts at:   1023    1437    1685    1720    1023 
   Size:        414     248      35    3310

  Fragments arranged by size: 

                3310     414     248      35
 
BanII G_rGCy'C

Cuts at:   1021    2051    1021 
   Size:       1030    2977
 
BbeI G_GCGC'C

Cuts at:   1441    1689    1441 
   Size:        248    3759
 
BbsI GAAGACnn'nnnn_

Cuts at:    277     277 
   Size:       4007
 
BciVI GTATCCnnnnn_n'

Cuts at:    471    1905    2666    4007     471 
   Size:       1434     761    1341     471

  Fragments arranged by size: 

                1434    1341     761     471
 
BclI T'GATC_A

Cuts at:   1527    1527 
   Size:       4007
 
BglII A'GATC_T

Cuts at:   1522    3559    1522 
   Size:       2037    1970
 
BlpI GC'TnA_GC

Cuts at:   1119    1119 
   Size:       4007
 
Bme1580I G_kGCm'C

Cuts at:    447    1632    1725    2781     447 
   Size:       1185      93    1056    1673

  Fragments arranged by size: 

                1673    1185    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    313    1219    1300    1635    3779     313 
   Size:        906      81     335    2144     541

  Fragments arranged by size: 

                2144     906     541     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2374    2374 
   Size:       4007
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    267     491     755     267 
   Size:        224     264    3519

  Fragments arranged by size: 

                3519     264     224
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1183    2062    2554    2852    1183 
   Size:        879     492     298    2338

  Fragments arranged by size: 

                2338     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3711    3711 
   Size:       4007
 
BsaAI yAC'GTr

Cuts at:   1360    1360 
   Size:       4007
 
BsaBI GATnn'nnATC

Cuts at:      6    1521    3558       6 
   Size:       1515    2037     455

  Fragments arranged by size: 

                2037    1515     455
 
BsaHI Gr'CG_yC

Cuts at:   1438    1686    2388    3884    1438 
   Size:        248     702    1496    1561

  Fragments arranged by size: 

                1561    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:    389    1196    1717    2395    2669    2816    3227     389 
   Size:        807     521     678     274     147     411    1169

  Fragments arranged by size: 

                1169     807     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    124     129     132     124 
   Size:          5       3    3999

  Fragments arranged by size: 

                3999       5       3
 
BseYI C'CCAG_C

Cuts at:   1432    2190    2767    3263    1432 
   Size:        758     577     496    2176

  Fragments arranged by size: 

                2176     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1549    1595    2803     104 
   Size:       1445      46    1208    1308

  Fragments arranged by size: 

                1445    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   1021    1799    1989    2781    1021 
   Size:        778     190     792    2247

  Fragments arranged by size: 

                2247     792     778     190
 
BsmI GAATG_Cn'

Cuts at:    800     800 
   Size:       4007
 
Bsp1286I G_dGCh'C

Cuts at:    447    1021    1632    1725    1799    1989    2051    2781     447 
   Size:        574     611      93      74     190      62     730    1673

  Fragments arranged by size: 

                1673     730     611     574     190      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:    389    3227     389 
   Size:       2838    1169
 
BspHI T'CATG_A

Cuts at:   3183    3183 
   Size:       4007
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    767    1057    1271    1573    1954     767 
   Size:        290     214     302     381    2820

  Fragments arranged by size: 

                2820     381     302     290     214
 
BsrBI CCG'CTC

Cuts at:     50    2299    2353    3837    4002      50 
   Size:       2249      54    1484     165      55

  Fragments arranged by size: 

                2249    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1919    1919 
   Size:       4007
 
BsrFI r'CCGG_y

Cuts at:   2005    2186    2395    2005 
   Size:        181     209    3617

  Fragments arranged by size: 

                3617     209     181
 
BssSI C'ACGA_G

Cuts at:   2278    2636    2278 
   Size:        358    3649
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1384    1384 
   Size:       4007
 
BstBI TT'CG_AA

Cuts at:   2368    2368 
   Size:       4007
 
BstYI r'GATC_y

Cuts at:      1    1032    1499    1522    1857    2103    3115    3201    3213 
   Size:       1031     467      23     335     246    1012      86      12

Cuts at:   3213    3559       1 
   Size:        346     449

  Fragments arranged by size: 

                1031    1012     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       4007
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1957    2138    2301    1957 
   Size:        181     163    3663

  Fragments arranged by size: 

                3663     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1250    1250 
   Size:       4007
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    801     836     801 
   Size:         35    3972
 
DraI TTT'AAA

Cuts at:    357    1006     357 
   Size:        649    3358
 
DrdI GACnn_nn'nnGTC

Cuts at:   1713    2571    1713 
   Size:        858    3149
 
EaeI y'GGCC_r

Cuts at:   1592    1766    2157    2184    3529    1592 
   Size:        174     391      27    1345    2070

  Fragments arranged by size: 

                2070    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1592    1592 
   Size:       4007
 
EarI CTCTTCn'nnn_

Cuts at:    347     467     540     925    2030    2240     347 
   Size:        120      73     385    1105     210    2114

  Fragments arranged by size: 

                2114    1105     385     210     120      73
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2215    2525    2671    3817    2215 
   Size:        310     146    1146    2405

  Fragments arranged by size: 

                2405    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1019    1019 
   Size:       4007
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    267     491     755    1832    2264    3011    3279     267 
   Size:        224     264    1077     432     747     268     995

  Fragments arranged by size: 

                1077     995     747     432     268     264     224
 
EcoNI CCTnn'n_nnAGG

Cuts at:    472     472 
   Size:       4007
 
EcoO109I rG'GnC_Cy

Cuts at:   1146    1146 
   Size:       4007
 
EcoRI G'AATT_C

Cuts at:   1011    1011 
   Size:       4007
 
EcoRV GAT'ATC

Cuts at:    772     772 
   Size:       4007
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2026    2026 
   Size:       4007
 
FspI TGC'GCA

Cuts at:   1788    1788 
   Size:       4007
 
HaeII r_GCGC'y

Cuts at:   1441    1689    2711    3690    1441 
   Size:        248    1022     979    1758

  Fragments arranged by size: 

                1758    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1849    1879    1881    1911    1849 
   Size:         30       2      30    3945

  Fragments arranged by size: 

                3945      30      30       2
 
HincII GTy'rAC

Cuts at:   1046    1046 
   Size:       4007
 
KasI G'GCGC_C

Cuts at:   1437    1685    1437 
   Size:        248    3759
 
KpnI G_GTAC'C

Cuts at:   1027    1027 
   Size:       4007
 
MluI A'CGCG_T

Cuts at:   3440    3440 
   Size:       4007
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    289     747    2678    2862     289 
   Size:        458    1931     184    1434

  Fragments arranged by size: 

                1931    1434     458     184
 
MscI TGG'CCA

Cuts at:   1768    1768 
   Size:       4007
 
MslI CAynn'nnrTG

Cuts at:    958    1996    2123     958 
   Size:       1038     127    2842

  Fragments arranged by size: 

                2842    1038     127
 
MspA1I CmG'CkG

Cuts at:   1118    1432    1792    2805    3050    1118 
   Size:        314     360    1013     245    2075

  Fragments arranged by size: 

                2075    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   2188    2188 
   Size:       4007
 
NarI GG'CG_CC

Cuts at:   1438    1686    1438 
   Size:        248    3759
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       4007
 
NgoMIV G'CCGG_C

Cuts at:   2186    2186 
   Size:       4007
 
NspI r_CATG'y

Cuts at:   1060    1560    2467    1060 
   Size:        500     907    2600

  Fragments arranged by size: 

                2600     907     500
 
PasI CC'CwG_GG

Cuts at:    168     168 
   Size:       4007
 
PciI A'CATG_T

Cuts at:   2463    2463 
   Size:       4007
 
PflMI CCAn_nnn'nTGG

Cuts at:    468     468 
   Size:       4007
 
PmeI GTTT'AAAC

Cuts at:   1006    1006 
   Size:       4007
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3146    3178    3146 
   Size:         32    3975
 
PsiI TTA'TAA

Cuts at:    997     997 
   Size:       4007
 
PstI C_TGCA'G

Cuts at:   1054    1739    1054 
   Size:        685    3322
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4007
 
PvuII CAG'CTG

Cuts at:   1432    1792    1432 
   Size:        360    3647
 
RsrII CG'GwC_CG

Cuts at:   2202    2202 
   Size:       4007
 
SacI G_AGCT'C

Cuts at:   1021    1021 
   Size:       4007
 
SalI G'TCGA_C

Cuts at:   1044    1044 
   Size:       4007
 
SapI GCTCTTCn'nnn_

Cuts at:   2030    2240    2030 
   Size:        210    3797
 
SbfI CC_TGCA'GG

Cuts at:   1054    1054 
   Size:       4007
 
ScaI AGT'ACT

Cuts at:   3524    3524 
   Size:       4007
 
SexAI A'CCwGG_T

Cuts at:    155     155 
   Size:       4007
 
SfcI C'TryA_G

Cuts at:     33    1050    1735    2728    2919      33 
   Size:       1017     685     993     191    1121

  Fragments arranged by size: 

                1121    1017     993     685     191
 
SfoI GGC'GCC

Cuts at:   1439    1687    1439 
   Size:        248    3759
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4007
 
SmaI CCC'GGG

Cuts at:   1029    1029 
   Size:       4007
 
SmlI C'TyrA_G

Cuts at:   1162    2077    2569    2831    1162 
   Size:        915     492     262    2338

  Fragments arranged by size: 

                2338     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4007
 
SphI G_CATG'C

Cuts at:   1060    1560    1060 
   Size:        500    3507
 
SspI AAT'ATT

Cuts at:    742     742 
   Size:       4007
 
StuI AGG'CCT

Cuts at:    184     184 
   Size:       4007
 
StyI C'CwwG_G

Cuts at:    107     873    1141    1495     107 
   Size:        766     268     354    2619

  Fragments arranged by size: 

                2619     766     354     268
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1590    1723    2182    2391    1590 
   Size:        133     459     209    3206

  Fragments arranged by size: 

                3206     459     209     133
 
TatI w'GTAC_w

Cuts at:   3522    3522 
   Size:       4007
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    973    1005    3524    3556     973 
   Size:         32    2519      32    1424

  Fragments arranged by size: 

                2519    1424      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1804    1804 
   Size:       4007
 
XbaI T'CTAG_A

Cuts at:   1038    1038 
   Size:       4007
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1502    1502 
   Size:       4007
 
XmaI C'CCGG_G

Cuts at:   1027    1027 
   Size:       4007
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AlfI
    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI     BbsI    BciVI     BclI    BglII     BlpI Bme1580I
     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI
    BstYI     BtgI    BtgZI     BtsI    CspCI     DraI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NspI     PasI     PciI    PflMI     PmeI     PpiI     PsiI
     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI
     ScaI    SexAI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI
     SphI     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     AleI     AloI     ApaI     AscI
    AvrII     BaeI    BbvCI     BcgI    BfrBI     BglI    BmgBI     BmtI
   Bpu10I    BsaXI     BsgI    BsiWI    BsmBI    BsrGI   BssHII   BstEII
    BstXI  BstZ17I   Bsu36I     ClaI   DraIII     FseI    FspAI  HindIII
     HpaI     MfeI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PfoI     PmlI    PpuMI    PshAI   PspOMI    PspXI     PsrI    SacII
    SanDI     SfiI    SgrAI    SnaBI     SrfI     SwaI     XhoI     XmnI
     ZraI
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