Restriction map for pF1KB8365
##################################
# NO CUT ENZYMES (  108 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AgeI        AhdI        AocI        
ApoI        AseI        AsnI        Asp718I     AspEI       
AspI        BamHI       BbrPI       BciVI       BclI        
BfrI        BsaAI       BsaBI       BsaI        BsaWI       
BseAI       BsmAI       BsmBI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspMI       BsrDI       
BsrGI       Bst1107I    BstAPI      BstBI       BstXI       
BstZ17I     Bsu36I      ClaI        CpoI        DraI        
DraIII      Eam1105I    Eco81I      EcoNI       EcoRI       
EcoRV       EcoT22I     FbaI        HincII      HindII      
HindIII     HpaI        KpnI        KspI        MamI        
MfeI        MluI        MroI        MseI        MunI        
NdeI        NheI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PstI        PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfcI        SfiI        SfuI        SgrAI       
SmlI        SnaBI       SpeI        Sse8387I    SspBI       
SspI        SwaI        TfiI        Tru9I       Tsp509I     
Tth111I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

Alw44I        937  GTGCAC               G^TGCAC
AlwI          596  GGATC                GGATC(4/5)
ApaLI         937  GTGCAC               G^TGCAC
AscI          964  GGCGCGCC             GG^CGCGCC
Asp700I       789  GAAGCGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         937  GTGCAC               G[AT]GC[AT]^C
AviII         351  TGCGCA               TGC^GCA
AvrII         216  CCTAGG               C^CTAGG
BalI          542  TGGCCA               TGG^CCA
BbvCI        1355  CCTCAGC              CCTCAGC(-5/-2)
BcgI          752  GCAACATGTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII         584  AGATCT               A^GATCT
BlnI          216  CCTAGG               C^CTAGG
BsiHKAI       937  GTGCAC               G[AT]GC[AT]^C
BsiWI         425  CGTACG               C^GTACG
BssHII        965  GCGCGC               G^CGCGC
BssSI        1144  CACGAG               CACGAG(-5/-1)
BstEII       1329  GGTGACC              G^GT[ACGT]ACC
BstPI        1329  GGTGACC              G^GT[ACGT]ACC
BstYI         584  AGATCT               [GA]^GATC[CT]
EarI          607  CTCTTC               CTCTTC(1/4)
EcoO65I      1329  GGTGACC              G^GT[ACGT]ACC
FseI          222  GGCCGGCC             GGCCGG^CC
FspI          351  TGCGCA               TGC^GCA
Ksp632I       607  CTCTTC               CTCTTC(1/4)
MflI          584  AGATCT               [GA]^GATC[CT]
MluNI         542  TGGCCA               TGG^CCA
MscI          542  TGGCCA               TGG^CCA
NotI          391  GCGGCCGC             GC^GGCCGC
PvuII        1153  CAGCTG               CAG^CTG
SfaNI         567  GATGC                GCATC(5/9)
SphI          251  GCATGC               GCATG^C
XcmI          368  CCATGAATGGCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         584  AGATCT               [GA]^GATC[CT]
XmnI          789  GAAGCGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AflIII        194  ACATGT               A^C[GA][CT]GT
              755  ACATGT              
Aor51HI       635  AGCGCT               AGC^GCT
              791  AGCGCT              
BbsI          945  GAAGAC               GAAGAC(2/6)
             1047  GAAGAC              
BglI          326  GCCCGAGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              452  GCCGCGCGGGC         
BlpI          321  GCTGAGC              GC^T[ACGT]AGC
              576  GCTGAGC             
BpmI         1052  CTCCAG               CTGGAG(16/14)
             1363  CTGGAG              
Bpu1102I      321  GCTGAGC              GC^T[ACGT]AGC
              576  GCTGAGC             
BpuAI         945  GAAGAC               GAAGAC(2/6)
             1047  GAAGAC              
BsgI          736  CTGCAC               GTGCAG(16/14)
             1156  CTGCAC              
BsiEI         293  CGGCCG               CG[GA][CT]^CG
              392  CGGCCG              
BspLU11I      194  ACATGT               A^CATGT
              755  ACATGT              
BstF5I        546  CATCC                GGATG(2/0)
              651  CATCC               
CelII         321  GCTGAGC              GC^T[ACGT]AGC
              576  GCTGAGC             
DpnII         585  GATC                 ^GATC
              597  GATC                
DrdI          177  GACCCCGGCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              676  GACTGCTTCGTC        
EagI          293  CGGCCG               C^GGCCG
              392  CGGCCG              
EclXI         293  CGGCCG               C^GGCCG
              392  CGGCCG              
Eco47III      635  AGCGCT               AGC^GCT
              791  AGCGCT              
Eco52I        293  CGGCCG               C^GGCCG
              392  CGGCCG              
FokI          546  CATCC                GGATG(9/13)
              651  CATCC               
MaeII         481  ACGT                 A^CGT
             1294  ACGT                
MaeIII        301  GTGAC                ^GT[ACGT]AC
             1330  GTGAC               
MboI          585  GATC                 ^GATC
              597  GATC                
MspA1I        852  CAGCGG               C[AC]G^C[GT]G
             1153  CAGCTG              
NdeII         585  GATC                 ^GATC
              597  GATC                
PflMI          35  CCAGCGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              996  CCAGACTCTGG         
PpuMI         884  AGGACCC              [GA]G^G[AT]CC[CT]
             1169  GGGACCC             
Sau3AI        585  GATC                 ^GATC
              597  GATC                
StuI           71  AGGCCT               AGG^CCT
              108  AGGCCT              
TaqI          761  TCGA                 T^CGA
             1349  TCGA                
Tsp45I        301  GTGAC                ^GT[GC]AC
             1330  GTGAC               
TthHB8I       761  TCGA                 T^CGA
             1349  TCGA                
Van91I         35  CCAGCGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              996  CCAGACTCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   82 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwNI       ApaI        AvaI        AvaII       BanI        
BanII       BbeI        BbvI        BcnI        BfaI        
BmyI        BsaHI       BsaJI       BseRI       BsiYI       
BslI        BsmFI       BsoBI       Bsp1286I    BsrBI       
BsrFI       BsrI        BssKI       BstNI       BstUI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DraII       DsaI        EaeI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        KasI        
MaeI        MboII       MnlI        MslI        MspI        
MvaI        MvnI        MwoI        NaeI        NarI        
NciI        NcoI        NgoMIV      NlaIII      NlaIV       
NspI        PleI        RsaI        Sau96I      ScrFI       
SfoI        SmaI        StyI        TseI        TspRI       
XmaCI       XmaI        
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