Restriction map for pF1KB8365
 (Circular) (Six-base) MAPSORT of: pF1KB8365.seq  Check: 9449  from: 1  to: 4529



pF1KB8365  4529 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:28  ..

AccI GT'mk_AC

Cuts at:   1567    1567 
   Size:       4529
 
Acc65I G'GTAC_C

Cuts at:   1545    1545 
   Size:       4529
 
AclI AA'CG_TT

Cuts at:   4369    4369 
   Size:       4529
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   2354    2786    3533    3801    2354 
   Size:        432     747     268    3082

  Fragments arranged by size: 

                3082     747     432     268
 
AfeI AGC'GCT

Cuts at:    745     901     745 
   Size:        156    4373
 
AflIII A'CryG_T

Cuts at:    302     863    1881    2985    3962     302 
   Size:        561    1018    1104     977     869

  Fragments arranged by size: 

                1104    1018     977     869     561
 
AgeI A'CCGG_T

Cuts at:   2917    2917 
   Size:       4529
 
AlwNI CAG_nnn'CTG

Cuts at:    149    1110    1377    1834    3401     149 
   Size:        961     267     457    1567    1277

  Fragments arranged by size: 

                1567    1277     961     457     267
 
ApaI G_GGCC'C

Cuts at:    493    1091    1199     493 
   Size:        598     108    3823

  Fragments arranged by size: 

                3823     598     108
 
ApaLI G'TGCA_C

Cuts at:   1045    3299    1045 
   Size:       2254    2275
 
ApoI r'AATT_y

Cuts at:   1533    3882    4138    1533 
   Size:       2349     256    1924

  Fragments arranged by size: 

                2349    1924     256
 
AscI GG'CGCG_CC

Cuts at:   1073    1073 
   Size:       4529
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4529
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4529
 
AvaI C'yCGr_G

Cuts at:    318     435    1084    1198    1549     318 
   Size:        117     649     114     351    3298

  Fragments arranged by size: 

                3298     649     351     117     114
 
AvrII C'CTAG_G

Cuts at:    324     324 
   Size:       4529
 
BamHI G'GATC_C

Cuts at:   1554    1554 
   Size:       4529
 
BanI G'GyrC_C

Cuts at:    379     448     663     964    1003    1141    1245    1545    1959 
   Size:         69     215     301      39     138     104     300     414

Cuts at:   1959    2207    2242     379 
   Size:        248      35    2666

  Fragments arranged by size: 

                2666     414     301     300     248     215     138     104
                  69      39      35
 
BanII G_rGCy'C

Cuts at:    226     373     436     493     526     832    1091    1199    1453 
   Size:        147      63      57      33     306     259     108     254

Cuts at:   1453    1463    1543    2573     226 
   Size:         10      80    1030    2182

  Fragments arranged by size: 

                2182    1030     306     259     254     147     108      80
                  63      57      33      10
 
BbeI G_GCGC'C

Cuts at:    383     452     667     968    1007    1145    1963    2211     383 
   Size:         69     215     301      39     138     818     248    2701

  Fragments arranged by size: 

                2701     818     301     248     215     138      69      39
            
 
BbsI GAAGACnn'nnnn_

Cuts at:   1060    1162    1060 
   Size:        102    4427
 
BbvCI CC'TCA_GC

Cuts at:   1464    1464 
   Size:       4529
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    849     883     849 
   Size:         34    4495
 
BciVI GTATCCnnnnn_n'

Cuts at:   2427    3188    4529    2427 
   Size:        761    1341    2427

  Fragments arranged by size: 

                2427    1341     761
 
BclI T'GATC_A

Cuts at:   2049    2049 
   Size:       4529
 
BglI GCCn_nnn'nGGC

Cuts at:    440     566     440 
   Size:        126    4403
 
BglII A'GATC_T

Cuts at:    692    2044    4081     692 
   Size:       1352    2037    1140

  Fragments arranged by size: 

                2037    1352    1140
 
BlpI GC'TnA_GC

Cuts at:    430     685    1641     430 
   Size:        255     956    3318

  Fragments arranged by size: 

                3318     956     255
 
Bme1580I G_kGCm'C

Cuts at:    493    1049    1091    1199    1220    2154    2247    3303     493 
   Size:        556      42     108      21     934      93    1056    1719

  Fragments arranged by size: 

                1719    1056     934     556     108      93      42      21
            
 
BmrI ACTGGGnnnn_n'

Cuts at:   1741    1822    2157    4301    1741 
   Size:         81     335    2144    1969

  Fragments arranged by size: 

                2144    1969     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    201    2896     201 
   Size:       2695    1834
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1145    1492    1145 
   Size:        347    4182
 
Bpu10I CC'TnA_GC

Cuts at:   1464    1464 
   Size:       4529
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1705    2584    3076    3374    1705 
   Size:        879     492     298    2860

  Fragments arranged by size: 

                2860     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   4233    4233 
   Size:       4529
 
BsaAI yAC'GTr

Cuts at:   1882    1882 
   Size:       4529
 
BsaBI GATnn'nnATC

Cuts at:      6    2043    4080       6 
   Size:       2037    2037     455

  Fragments arranged by size: 

                2037    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    292     380     449     581     664     965    1004    1142    1960 
   Size:         88      69     132      83     301      39     138     818

Cuts at:   1960    2208    2910    4406     292 
   Size:        248     702    1496     415

  Fragments arranged by size: 

                1496     818     702     415     301     248     138     132
                  88      83      69      39
 
BsaWI w'CCGG_w

Cuts at:   1718    2239    2917    3191    3338    3749    1718 
   Size:        521     678     274     147     411    2498

  Fragments arranged by size: 

                2498     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    838     868    1291    1321    1465    1495     838 
   Size:         30     423      30     144      30    3872

  Fragments arranged by size: 

                3872     423     144      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    177    1158    1320     177 
   Size:        981     162    3386

  Fragments arranged by size: 

                3386     981     162
 
BseYI C'CCAG_C

Cuts at:    667    1015    1259    1954    2712    3289    3785     667 
   Size:        348     244     695     758     577     496    1411

  Fragments arranged by size: 

                1411     758     695     577     496     348     244
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    829    1249     829 
   Size:        420    4109
 
BsiEI CG_ry'CG

Cuts at:    104     404     503    2071    2117    3325     104 
   Size:        300      99    1568      46    1208    1308

  Fragments arranged by size: 

                1568    1308    1208     300      99      46
 
BsiHKAI G_wGCw'C

Cuts at:   1049    1543    2321    2511    3303    1049 
   Size:        494     778     190     792    2275

  Fragments arranged by size: 

                2275     792     778     494     190
 
BsiWI C'GTAC_G

Cuts at:    533     533 
   Size:       4529
 
Bsp1286I G_dGCh'C

Cuts at:    226     373     436     493     526     832    1049    1091    1199 
   Size:        147      63      57      33     306     217      42     108

Cuts at:   1199    1220    1453    1463    1543    2154    2247    2321    2511 
   Size:         21     233      10      80     611      93      74     190

Cuts at:   2511    2573    3303     226 
   Size:         62     730    1452

  Fragments arranged by size: 

                1452     730     611     306     233     217     190     147
                 108      93      80      74      63      62      57      42
                  33      21      10
 
BspEI T'CCGG_A

Cuts at:   3749    3749 
   Size:       4529
 
BspHI T'CATG_A

Cuts at:   3705    3705 
   Size:       4529
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1579    1793    2095    2476    1579 
   Size:        214     302     381    3632

  Fragments arranged by size: 

                3632     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     275     440     947    2821    2875    4359    4524      50 
   Size:        225     165     507    1874      54    1484     165      55

  Fragments arranged by size: 

                1874    1484     507     225     165     165      55      54
            
 
BsrDI GCAATG_nn'

Cuts at:   2441    2441 
   Size:       4529
 
BsrFI r'CCGG_y

Cuts at:    331     355     403     728     928    1057    1077    1476    2527 
   Size:         24      48     325     200     129      20     399    1051

Cuts at:   2527    2708    2917     331 
   Size:        181     209    1943

  Fragments arranged by size: 

                1943    1051     399     325     209     200     181     129
                  48      24      20
 
BssHII G'CGCG_C

Cuts at:   1073    1073 
   Size:       4529
 
BssSI C'ACGA_G

Cuts at:   1252    2800    3158    1252 
   Size:       1548     358    2623

  Fragments arranged by size: 

                2623    1548     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1906    1906 
   Size:       4529
 
BstBI TT'CG_AA

Cuts at:   2890    2890 
   Size:       4529
 
BstEII G'GTnAC_C

Cuts at:   1437    1437 
   Size:       4529
 
BstYI r'GATC_y

Cuts at:      1     692    1554    2021    2044    2379    2625    3637    3723 
   Size:        691     862     467      23     335     246    1012      86

Cuts at:   3723    3735    4081       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     862     691     467     449     346     335     246
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    416     452     527     647     416 
   Size:         36      75     120    4298

  Fragments arranged by size: 

                4298     120      75      36
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2479    2660    2823    2479 
   Size:        181     163    4185

  Fragments arranged by size: 

                4185     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1375    1772    1375 
   Size:        397    4132
 
DraI TTT'AAA

Cuts at:   1528    1528 
   Size:       4529
 
DrdI GACnn_nn'nnGTC

Cuts at:    291     790    2235    3093     291 
   Size:        499    1445     858    1727

  Fragments arranged by size: 

                1727    1445     858     499
 
EaeI y'GGCC_r

Cuts at:    401     413     500     650    1059    2114    2288    2679    2706 
   Size:         12      87     150     409    1055     174     391      27

Cuts at:   2706    4051     401 
   Size:       1345     879

  Fragments arranged by size: 

                1345    1055     879     409     391     174     150      87
                  27      12
 
EagI C'GGCC_G

Cuts at:    401     500    2114     401 
   Size:         99    1614    2816

  Fragments arranged by size: 

                2816    1614      99
 
EarI CTCTTCn'nnn_

Cuts at:    721    2552    2762     721 
   Size:       1831     210    2488

  Fragments arranged by size: 

                2488    1831     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    751    2737    3047    3193    4339     751 
   Size:       1986     310     146    1146     941

  Fragments arranged by size: 

                1986    1146     941     310     146
 
EcoICRI GAG'CTC

Cuts at:   1541    1541 
   Size:       4529
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1145    1492    2354    2786    3533    3801    1145 
   Size:        347     862     432     747     268    1873

  Fragments arranged by size: 

                1873     862     747     432     347     268
 
EcoO109I rG'GnC_Cy

Cuts at:    490     993    1278    1668     490 
   Size:        503     285     390    3351

  Fragments arranged by size: 

                3351     503     390     285
 
EcoRI G'AATT_C

Cuts at:   1533    1533 
   Size:       4529
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1371    2548    1371 
   Size:       1177    3352
 
FseI GG_CCGG'CC

Cuts at:    335     335 
   Size:       4529
 
FspI TGC'GCA

Cuts at:    461    2310     461 
   Size:       1849    2680
 
HaeII r_GCGC'y

Cuts at:    383     430     452     667     747     903     968    1007    1022 
   Size:         47      22     215      80     156      65      39      15

Cuts at:   1022    1145    1207    1433    1963    2211    3233    4212     383 
   Size:        123      62     226     530     248    1022     979     700

  Fragments arranged by size: 

                1022     979     700     530     248     226     215     156
                 123      80      65      62      47      39      22      15
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   2371    2401    2403    2433    2371 
   Size:         30       2      30    4467

  Fragments arranged by size: 

                4467      30      30       2
 
HincII GTy'rAC

Cuts at:   1568    1568 
   Size:       4529
 
KasI G'GCGC_C

Cuts at:    379     448     663     964    1003    1141    1959    2207     379 
   Size:         69     215     301      39     138     818     248    2701

  Fragments arranged by size: 

                2701     818     301     248     215     138      69      39
            
 
KpnI G_GTAC'C

Cuts at:   1549    1549 
   Size:       4529
 
MluI A'CGCG_T

Cuts at:   3962    3962 
   Size:       4529
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    569    3200    3384     569 
   Size:       2631     184    1714

  Fragments arranged by size: 

                2631    1714     184
 
MscI TGG'CCA

Cuts at:    652    2290     652 
   Size:       1638    2891
 
MslI CAynn'nnrTG

Cuts at:    247     253     646    1040    2518    2645     247 
   Size:          6     393     394    1478     127    2131

  Fragments arranged by size: 

                2131    1478     394     393     127       6
 
MspA1I CmG'CkG

Cuts at:    962    1263    1640    1954    2314    3327    3572     962 
   Size:        301     377     314     360    1013     245    1919

  Fragments arranged by size: 

                1919    1013     377     360     314     301     245
 
NaeI GCC'GGC

Cuts at:    333     357     405     930    1079    1478    2710     333 
   Size:         24      48     525     149     399    1232    2152

  Fragments arranged by size: 

                2152    1232     525     399     149      48      24
 
NarI GG'CG_CC

Cuts at:    380     449     664     965    1004    1142    1960    2208     380 
   Size:         69     215     301      39     138     818     248    2701

  Fragments arranged by size: 

                2701     818     301     248     215     138      69      39
            
 
NcoI C'CATG_G

Cuts at:    416     452     527     647     416 
   Size:         36      75     120    4298

  Fragments arranged by size: 

                4298     120      75      36
 
NgoMIV G'CCGG_C

Cuts at:    331     355     403     928    1077    1476    2708     331 
   Size:         24      48     525     149     399    1232    2152

  Fragments arranged by size: 

                2152    1232     525     399     149      48      24
 
NotI GC'GGCC_GC

Cuts at:    500     500 
   Size:       4529
 
NspI r_CATG'y

Cuts at:    306     363     867    1582    2082    2989     306 
   Size:         57     504     715     500     907    1846

  Fragments arranged by size: 

                1846     907     715     504     500      57
 
PciI A'CATG_T

Cuts at:    302     863    2985     302 
   Size:        561    2122    1846

  Fragments arranged by size: 

                2122    1846     561
 
PflMI CCAn_nnn'nTGG

Cuts at:    149    1110     149 
   Size:        961    3568
 
PmeI GTTT'AAAC

Cuts at:   1528    1528 
   Size:       4529
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3668    3700    3668 
   Size:         32    4497
 
PpuMI rG'GwC_Cy

Cuts at:    993    1278     993 
   Size:        285    4244
 
PspOMI G'GGCC_C

Cuts at:    489    1087    1195     489 
   Size:        598     108    3823

  Fragments arranged by size: 

                3823     598     108
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:    145     177     865     897     145 
   Size:         32     688      32    3777

  Fragments arranged by size: 

                3777     688      32      32
 
PstI C_TGCA'G

Cuts at:   1576    2261    1576 
   Size:        685    3844
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4529
 
PvuII CAG'CTG

Cuts at:   1263    1954    2314    1263 
   Size:        691     360    3478

  Fragments arranged by size: 

                3478     691     360
 
RsrII CG'GwC_CG

Cuts at:   2724    2724 
   Size:       4529
 
SacI G_AGCT'C

Cuts at:   1543    1543 
   Size:       4529
 
SalI G'TCGA_C

Cuts at:   1566    1566 
   Size:       4529
 
SanDI GG'GwC_CC

Cuts at:   1278    1278 
   Size:       4529
 
SapI GCTCTTCn'nnn_

Cuts at:   2552    2762    2552 
   Size:        210    4319
 
SbfI CC_TGCA'GG

Cuts at:   1576    1576 
   Size:       4529
 
ScaI AGT'ACT

Cuts at:   4046    4046 
   Size:       4529
 
SfcI C'TryA_G

Cuts at:     33    1572    2257    3250    3441      33 
   Size:       1539     685     993     191    1121

  Fragments arranged by size: 

                1539    1121     993     685     191
 
SfoI GGC'GCC

Cuts at:    381     450     665     966    1005    1143    1961    2209     381 
   Size:         69     215     301      39     138     818     248    2701

  Fragments arranged by size: 

                2701     818     301     248     215     138      69      39
            
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4529
 
SmaI CCC'GGG

Cuts at:    320    1086    1200    1551     320 
   Size:        766     114     351    3298

  Fragments arranged by size: 

                3298     766     351     114
 
SmlI C'TyrA_G

Cuts at:   1684    2599    3091    3353    1684 
   Size:        915     492     262    2860

  Fragments arranged by size: 

                2860     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4529
 
SphI G_CATG'C

Cuts at:    363    1582    2082     363 
   Size:       1219     500    2810

  Fragments arranged by size: 

                2810    1219     500
 
StuI AGG'CCT

Cuts at:    181     218     181 
   Size:         37    4492
 
StyI C'CwwG_G

Cuts at:    324     416     452     527     647     968    1488    1663    2017 
   Size:         92      36      75     120     321     520     175     354

Cuts at:   2017     324 
   Size:       2836

  Fragments arranged by size: 

                2836     520     354     321     175     120      92      75
                  36
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    445    2112    2245    2704    2913     445 
   Size:       1667     133     459     209    2061

  Fragments arranged by size: 

                2061    1667     459     209     133
 
TatI w'GTAC_w

Cuts at:   4044    4044 
   Size:       4529
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    757     789     838     870    1291    1323    4046    4078     757 
   Size:         32      49      32     421      32    2723      32    1208

  Fragments arranged by size: 

                2723    1208     421      49      32      32      32      32
            
 
Tth111I GACn'n_nGTC

Cuts at:   2326    2326 
   Size:       4529
 
XbaI T'CTAG_A

Cuts at:   1560    1560 
   Size:       4529
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    483    2024     483 
   Size:       1541    2988
 
XmaI C'CCGG_G

Cuts at:    318    1084    1198    1549     318 
   Size:        766     114     351    3298

  Fragments arranged by size: 

                3298     766     351     114
 
XmnI GAAnn'nnTTC

Cuts at:    901     901 
   Size:       4529
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI    AlwNI
     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     DraI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI
     FalI     FseI     FspI    HaeII    Hin4I   HincII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NotI     NspI     PciI    PflMI     PmeI     PpiI    PpuMI
   PspOMI     PsrI     PstI     PvuI    PvuII    RsrII     SacI     SalI
    SanDI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI
     SmlI     SpeI     SphI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII    AflII     AhdI     AleI     AlfI     AloI     BaeI
    BfrBI    BmgBI     BmtI     BsmI    BsmBI    BsrGI    BstXI  BstZ17I
   Bsu36I     ClaI    CspCI   DraIII    EcoNI    EcoRV    FspAI  HindIII
     HpaI     MfeI     NdeI     NheI     NruI     NsiI     PacI     PasI
     PfoI     PmlI    PshAI     PsiI    PspXI    SacII    SexAI     SfiI
    SgrAI    SnaBI     SrfI     SspI     SwaI     XhoI     ZraI
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