Restriction map for pF1KB8362
 (Linear) (Six-base) MAPSORT of: KIBB8362.seq  Check: 3084  from: 1  to: 1329



KIBB8362.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:05  ..

AatII G_ACGT'C

Cuts at:      0     361     482     946    1329 
   Size:        361     121     464     383

  Fragments arranged by size: 

                 464     383     361     121
 
AccI GT'mk_AC

Cuts at:      0     395     640    1329 
   Size:        395     245     689

  Fragments arranged by size: 

                 689     395     245
 
Acc65I G'GTAC_C

Cuts at:      0     221    1329 
   Size:        221    1108
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1209    1329 
   Size:       1209     120
 
AflIII A'CryG_T

Cuts at:      0    1196    1329 
   Size:       1196     133
 
AleI CACnn'nnGTG

Cuts at:      0     602    1329 
   Size:        602     727
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1147    1329 
   Size:       1147     182
 
ApaI G_GGCC'C

Cuts at:      0     386    1329 
   Size:        386     943
 
AvaI C'yCGr_G

Cuts at:      0      84     378     679    1329 
   Size:         84     294     301     650

  Fragments arranged by size: 

                 650     301     294      84
 
BamHI G'GATC_C

Cuts at:      0     274    1329 
   Size:        274    1055
 
BanI G'GyrC_C

Cuts at:      0     221     561    1329 
   Size:        221     340     768

  Fragments arranged by size: 

                 768     340     221
 
BanII G_rGCy'C

Cuts at:      0     386     866    1028    1329 
   Size:        386     480     162     301

  Fragments arranged by size: 

                 480     386     301     162
 
BbvCI CC'TCA_GC

Cuts at:      0      91    1329 
   Size:         91    1238
 
BglI GCCn_nnn'nGGC

Cuts at:      0     523     719    1329 
   Size:        523     196     610

  Fragments arranged by size: 

                 610     523     196
 
Bme1580I G_kGCm'C

Cuts at:      0     386     566     680    1329 
   Size:        386     180     114     649

  Fragments arranged by size: 

                 649     386     180     114
 
BmgBI CAC'GTC

Cuts at:      0     177    1329 
   Size:        177    1152
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     242     705    1329 
   Size:        242     463     624

  Fragments arranged by size: 

                 624     463     242
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     607    1002    1165    1173    1218    1290    1329 
   Size:        607     395     163       8      45      72      39

  Fragments arranged by size: 

                 607     395     163      72      45      39       8
 
Bpu10I CC'TnA_GC

Cuts at:      0      91    1207    1329 
   Size:         91    1116     122

  Fragments arranged by size: 

                1116     122      91
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     535    1329 
   Size:        535     794
 
BsaAI yAC'GTr

Cuts at:      0     675    1329 
   Size:        675     654
 
BsaHI Gr'CG_yC

Cuts at:      0     358     479     943    1329 
   Size:        358     121     464     386

  Fragments arranged by size: 

                 464     386     358     121
 
BsaWI w'CCGG_w

Cuts at:      0     691    1329 
   Size:        691     638
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     310     340     358     388    1186    1216    1288    1318 
   Size:        310      30      18      30     798      30      72      30

Cuts at:   1318    1329 
   Size:         11

  Fragments arranged by size: 

                 798     310      72      30      30      30      30      18
                  11
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     148     405     624     999    1329 
   Size:        148     257     219     375     330

  Fragments arranged by size: 

                 375     330     257     219     148
 
BseYI C'CCAG_C

Cuts at:      0      26     706     717    1329 
   Size:         26     680      11     612

  Fragments arranged by size: 

                 680     612      26      11
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     235    1329 
   Size:        235    1094
 
BsiEI CG_ry'CG

Cuts at:      0     518    1181    1329 
   Size:        518     663     148

  Fragments arranged by size: 

                 663     518     148
 
BsiHKAI G_wGCw'C

Cuts at:      0      56    1329 
   Size:         56    1273
 
BsmI GAATG_Cn'

Cuts at:      0     996    1329 
   Size:        996     333
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     486    1329 
   Size:        486     843
 
Bsp1286I G_dGCh'C

Cuts at:      0      56     386     566     680     866    1028    1329 
   Size:         56     330     180     114     186     162     301

  Fragments arranged by size: 

                 330     301     186     180     162     114      56
 
BspEI T'CCGG_A

Cuts at:      0     691    1329 
   Size:        691     638
 
BsrDI GCAATG_nn'

Cuts at:      0    1020    1329 
   Size:       1020     309
 
BsrFI r'CCGG_y

Cuts at:      0     517     733     890    1329 
   Size:        517     216     157     439

  Fragments arranged by size: 

                 517     439     216     157
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     857    1329 
   Size:        857     472
 
BstEII G'GTnAC_C

Cuts at:      0    1314    1329 
   Size:       1314      15
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     604    1329 
   Size:        604     725
 
BstYI r'GATC_y

Cuts at:      0     274    1329 
   Size:        274    1055
 
BtgI C'CryG_G

Cuts at:      0     209     237     369     603    1322    1329 
   Size:        209      28     132     234     719       7

  Fragments arranged by size: 

                 719     234     209     132      28       7
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     479    1329 
   Size:        479     850
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1002    1037    1329 
   Size:       1002      35     292

  Fragments arranged by size: 

                1002     292      35
 
EaeI y'GGCC_r

Cuts at:      0     515    1178    1329 
   Size:        515     663     151

  Fragments arranged by size: 

                 663     515     151
 
EagI C'GGCC_G

Cuts at:      0     515    1178    1329 
   Size:        515     663     151

  Fragments arranged by size: 

                 663     515     151
 
EarI CTCTTCn'nnn_

Cuts at:      0     454     703    1329 
   Size:        454     249     626

  Fragments arranged by size: 

                 626     454     249
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      28    1329 
   Size:         28    1301
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     607    1002    1165    1173    1209    1218    1290    1329 
   Size:        607     395     163       8      36       9      72      39

  Fragments arranged by size: 

                 607     395     163      72      39      36       9       8
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0     382     383    1002    1329 
   Size:        382       1     619     327

  Fragments arranged by size: 

                 619     382     327       1
 
HincII GTy'rAC

Cuts at:      0     641    1329 
   Size:        641     688
 
KpnI G_GTAC'C

Cuts at:      0     225    1329 
   Size:        225    1104
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     253    1329 
   Size:        253    1076
 
MslI CAynn'nnrTG

Cuts at:      0     602    1261    1321    1329 
   Size:        602     659      60       8

  Fragments arranged by size: 

                 659     602      60       8
 
MspA1I CmG'CkG

Cuts at:      0     126    1329 
   Size:        126    1203
 
NaeI GCC'GGC

Cuts at:      0     519    1329 
   Size:        519     810
 
NcoI C'CATG_G

Cuts at:      0    1322    1329 
   Size:       1322       7
 
NgoMIV G'CCGG_C

Cuts at:      0     517    1329 
   Size:        517     812
 
NspI r_CATG'y

Cuts at:      0    1200    1245    1329 
   Size:       1200      45      84

  Fragments arranged by size: 

                1200      84      45
 
PasI CC'CwG_GG

Cuts at:      0     306     621    1329 
   Size:        306     315     708

  Fragments arranged by size: 

                 708     315     306
 
PciI A'CATG_T

Cuts at:      0    1196    1329 
   Size:       1196     133
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     980    1329 
   Size:        980     349
 
PpuMI rG'GwC_Cy

Cuts at:      0    1002    1329 
   Size:       1002     327
 
PshAI GACnn'nnGTC

Cuts at:      0    1197    1329 
   Size:       1197     132
 
PspOMI G'GGCC_C

Cuts at:      0     382    1329 
   Size:        382     947
 
PstI C_TGCA'G

Cuts at:      0     927    1329 
   Size:        927     402
 
SalI G'TCGA_C

Cuts at:      0     639    1329 
   Size:        639     690
 
SanDI GG'GwC_CC

Cuts at:      0    1002    1329 
   Size:       1002     327
 
SexAI A'CCwGG_T

Cuts at:      0     557    1329 
   Size:        557     772
 
SfcI C'TryA_G

Cuts at:      0     923    1329 
   Size:        923     406
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     523    1329 
   Size:        523     806
 
SmaI CCC'GGG

Cuts at:      0      86     380    1329 
   Size:         86     294     949

  Fragments arranged by size: 

                 949     294      86
 
SmlI C'TyrA_G

Cuts at:      0     550    1329 
   Size:        550     779
 
SspI AAT'ATT

Cuts at:      0    1047    1329 
   Size:       1047     282
 
StyI C'CwwG_G

Cuts at:      0     758    1322    1329 
   Size:        758     564       7

  Fragments arranged by size: 

                 758     564       7
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      18     189     613    1329 
   Size:         18     171     424     716

  Fragments arranged by size: 

                 716     424     171      18
 
TatI w'GTAC_w

Cuts at:      0     683    1329 
   Size:        683     646
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     310     342     358     390     933     965    1186    1218 
   Size:        310      32      16      32     543      32     221      32

Cuts at:   1218    1258    1288    1290    1320    1329 
   Size:         40      30       2      30       9

  Fragments arranged by size: 

                 543     310     221      40      32      32      32      32
                  30      30      16       9       2
 
Tth111I GACn'n_nGTC

Cuts at:      0    1006    1329 
   Size:       1006     323
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     714    1329 
   Size:        714     615
 
XmaI C'CCGG_G

Cuts at:      0      84     378    1329 
   Size:         84     294     951

  Fragments arranged by size: 

                 951     294      84
 
ZraI GAC'GTC

Cuts at:      0     359     480     944    1329 
   Size:        359     121     464     385

  Fragments arranged by size: 

                 464     385     359     121
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AcuI   AflIII     AleI     AlfI     ApaI
     AvaI    BamHI     BanI    BanII    BbvCI     BglI Bme1580I    BmgBI
     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspEI    BsrDI    BsrFI   BstAPI   BstEII    BstXI    BstYI     BtgI
    BtgZI    CspCI     EaeI     EagI     EarI     EciI  Eco57MI EcoO109I
   HincII     KpnI     MmeI     MslI   MspA1I     NaeI     NcoI   NgoMIV
     NspI     PasI     PciI    PflMI    PpuMI    PshAI   PspOMI     PstI
     SalI    SanDI    SexAI     SfcI     SfiI     SmaI     SmlI     SspI
     StyI    TaqII     TatI     TstI  Tth111I     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

     AarI     AclI     AfeI    AflII     AgeI     AhdI     AloI    AlwNI
    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI     BbeI
     BbsI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI     BmrI
     BmtI     BsaI    BsaBI    BsiWI    BspHI    BspMI    BsrBI    BsrGI
   BssHII    BssSI    BstBI  BstZ17I   Bsu36I     BtsI     ClaI     DraI
   DraIII     DrdI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I  HindIII     HpaI     KasI     MfeI
     MluI     MscI     NarI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PfoI     PmeI     PmlI     PpiI     PsiI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SapI     SbfI     ScaI
     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     StuI
     SwaI     XbaI     XhoI     XmnI
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