Restriction map for pF1KB8361
##################################
# NO CUT ENZYMES (  184 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvaII       
AviII       AvrII       BalI        BanII       BbeI        
BbrPI       BbvCI       BcgI        BciVI       BcnI        
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaHI       BsaI        BsaWI       BseAI       
BseRI       BsgI        BsiEI       BsiWI       BsmAI       
BsmBI       BsmFI       BsmI        Bsp1407I    BspDI       
BspEI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssKI       BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstNI       
BstPI       BstUI       BstXI       BstZ17I     Bsu36I      
CelII       CfoI        Cfr10I      ClaI        CpoI        
DraI        DraII       DraIII      DrdI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoO109I    EcoO65I     EcoRI       
EcoRII      EcoRV       EcoT22I     FauI        FseI        
FspI        HaeII       HapII       HgaI        HhaI        
Hin1I       HinP1I      HindIII     HpaI        HpaII       
KasI        KpnI        Ksp632I     KspI        MfeI        
MluI        MluNI       MroI        MscI        MseI        
MslI        MspA1I      MspI        MunI        MvaI        
MvnI        NaeI        NarI        NciI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
PvuII       RsrII       SacI        SacII       SalI        
SapI        SbfI        ScrFI       SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        TaqI        Tru9I       
Tth111I     TthHB8I     Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AciI          110  CCGC                 CCGC(-3/-1)
AluI          253  AGCT                 AG^CT
AspHI         417  GAGCAC               G[AT]GC[AT]^C
AvaI          270  CTCGGG               C^[CT]CG[GA]G
BamHI         160  GGATCC               G^GATCC
BanI          238  GGCACC               G^G[CT][GA]CC
BbsI            3  GAAGAC               GAAGAC(2/6)
BfaI          356  CTAG                 C^TAG
BglI          137  GCCACTGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI          417  GAGCAC               G[AGT]GC[ACT]^C
BpmI          220  CTGGAG               CTGGAG(16/14)
BpuAI           3  GAAGAC               GAAGAC(2/6)
BsaBI          27  GATCATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI       417  GAGCAC               G[AT]GC[AT]^C
BsoBI         270  CTCGGG               C^[CT]CG[GA]G
Bsp1286I      417  GAGCAC               G[AGT]GC[ACT]^C
BsrI          387  CCAGT                ACTGG(1/-1)
BstYI         160  GGATCC               [GA]^GATC[CT]
Cac8I          48  GCAGGC               GC[ACGT]^[ACGT]GC
Cfr13I         51  GGCCC                G^G[ACGT]CC
DsaI           54  CCATGG               C^C[GA][CT]GG
EcoNI          42  CCTACTGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
MaeI          356  CTAG                 C^TAG
MaeIII        193  GTCAC                ^GT[ACGT]AC
MamI           27  GATCATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII           3  GAAGA                GAAGA(8/7)
MflI          160  GGATCC               [GA]^GATC[CT]
NcoI           54  CCATGG               C^CATGG
PleI          301  GACTC                GAGTC(4/5)
Sau96I         51  GGCCC                G^G[ACGT]CC
ScaI          389  AGTACT               AGT^ACT
SfaNI         172  GATGC                GCATC(5/9)
TfiI          281  GAATC                G^A[AT]TC
Tsp45I        193  GTCAC                ^GT[GC]AC
XhoII         160  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AfaI          384  GTAC                 GT^AC
              390  GTAC                
BclI           26  TGATCA               T^GATCA
               83  TGATCA              
BsaJI          54  CCATGG               C^C[ACGT][ACGT]GG
              167  CCAAGG              
BsiYI          12  CCTACTGTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
               42  CCTACTGCAGG         
BslI           12  CCTACTGTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
               42  CCTACTGCAGG         
BspHI          29  TCATGA               T^CATGA
              198  TCATGA              
BstF5I        171  GGATG                GGATG(2/0)
              232  GGATG               
DdeI          115  CTCAG                C^T[ACGT]AG
              250  CTGAG               
EcoT14I        54  CCATGG               C^C[AT][AT]GG
              167  CCAAGG              
FbaI           26  TGATCA               T^GATCA
               83  TGATCA              
FokI          171  GGATG                GGATG(9/13)
              232  GGATG               
HaeIII         40  GGCC                 GG^CC
               51  GGCC                
HincII         90  GTTGAC               GT[CT]^[GA]AC
              314  GTCAAC              
HindII         90  GTTGAC               GT[CT]^[GA]AC
              314  GTCAAC              
HinfI         281  GAATC                G^A[ACGT]TC
              301  GACTC               
HphI           66  GGTGA                GGTGA(8/7)
              284  TCACC               
MaeII         216  ACGT                 A^CGT
              228  ACGT                
NlaIV         160  GGATCC               GG[ACGT]^[ACGT]CC
              238  GGCACC              
RcaI           29  TCATGA               T^CATGA
              198  TCATGA              
RsaI          384  GTAC                 GT^AC
              390  GTAC                
StyI           54  CCATGG               C^C[AT][AT]GG
              167  CCAAGG              


#########################################
# 3 OR MORE CUT ENZYMES (   18 enzymes) #
#########################################

AcsI        AlwI        ApoI        BbvI        DpnII       
Fnu4HI      ItaI        MboI        MnlI        MwoI        
NdeII       NlaIII      PstI        Sau3AI      SfcI        
TseI        Tsp509I     TspRI       
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