Restriction map for pF1KB8353
##################################
# NO CUT ENZYMES (  124 enzymes) #
##################################

AatII       AclI        AcsI        AcyI        AflII       
AflIII      AgeI        AhdI        AlwI        AlwNI       
Aor51HI     ApaI        ApoI        AscI        AseI        
AsnI        Asp700I     AspEI       AspI        AviII       
AvrII       BamHI       BbeI        BbrPI       BbsI        
BcgI        BciVI       BfaI        BfrI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaHI       BsaI        BsiWI       BsmI        BspDI       
BspHI       BspLU11I    BsrBI       BsrFI       BsrI        
BssHII      BstAPI      BstBI       BstYI       CelII       
Cfr10I      ClaI        CpoI        DraI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoRI       EcoRV       EcoT22I     FseI        
FspI        Hin1I       HincII      HindII      HpaI        
KasI        KspI        MaeI        MfeI        MflI        
MluI        MunI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        Sse8387I    SspI        
SwaI        TfiI        Tth111I     Van91I      XbaI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   58 enzymes) #
###################################

Acc65I        908  GGTACC               G^GTACC
AccIII        857  TCCGGA               T^CCGGA
Alw44I        220  GTGCAC               G^TGCAC
AocI          899  CCTCAGG              CC^T[ACGT]AGG
ApaLI         220  GTGCAC               G^TGCAC
Asp718I       908  GGTACC               G^GTACC
AvaI          787  CTCGAG               C^[CT]CG[GA]G
BalI           46  TGGCCA               TGG^CCA
BbvCI         330  GCTGAGG              CCTCAGC(-5/-2)
BclI          134  TGATCA               T^GATCA
BcnI          876  CCGGG                CC^[GC]GG
BsaBI         541  GATGCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         857  TCCGGA               [AT]^CCGG[AT]
BseAI         857  TCCGGA               T^CCGGA
BseRI         202  GAGGAG               GAGGAG(10/8)
BsgI          316  GTGCAG               GTGCAG(16/14)
BsiEI         438  CGACCG               CG[GA][CT]^CG
BsmBI         993  CGTCTC               CGTCTC(1/5)
BsoBI         787  CTCGAG               C^[CT]CG[GA]G
Bsp1407I      593  TGTACA               T^GTACA
BspEI         857  TCCGGA               T^CCGGA
BsrGI         593  TGTACA               T^GTACA
BssSI         225  CACGAG               CACGAG(-5/-1)
Bst1107I      763  GTATAC               GTA^TAC
BstEII        894  GGTGACC              G^GT[ACGT]ACC
BstPI         894  GGTGACC              G^GT[ACGT]ACC
BstZ17I       763  GTATAC               GTA^TAC
Bsu36I        899  CCTCAGG              CC^T[ACGT]AGG
EarI          151  GAAGAG               CTCTTC(1/4)
Eco81I        899  CCTCAGG              CC^T[ACGT]AGG
EcoNI         702  CCTGGATAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       894  GGTGACC              G^GT[ACGT]ACC
EcoT14I       801  CCTTGG               C^C[AT][AT]GG
FbaI          134  TGATCA               T^GATCA
HaeII         230  GGCGCT               [GA]GCGC^[CT]
HgaI          992  GCGTC                GACGC(5/10)
HindIII       157  AAGCTT               A^AGCTT
KpnI          908  GGTACC               GGTAC^C
Ksp632I       151  GAAGAG               CTCTTC(1/4)
MamI          541  GATGCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI          46  TGGCCA               TGG^CCA
MroI          857  TCCGGA               T^CCGGA
MscI           46  TGGCCA               TGG^CCA
NciI          876  CCGGG                CC^[GC]GG
PaeR7I        787  CTCGAG               C^TCGAG
PpuMI         206  AGGACCT              [GA]G^G[AT]CC[CT]
PstI         1031  CTGCAG               CTGCA^G
PvuII          16  CAGCTG               CAG^CTG
SacI          244  GAGCTC               GAGCT^C
SfcI         1031  CTGCAG               C^T[GA][CT]AG
SphI          173  GCATGC               GCATG^C
SspBI         593  TGTACA               T^GTACA
StuI          194  AGGCCT               AGG^CCT
StyI          801  CCTTGG               C^C[AT][AT]GG
TaqI          788  TCGA                 T^CGA
Tsp509I       139  AATT                 ^AATT
TthHB8I       788  TCGA                 T^CGA
XhoI          787  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AccI          607  GTCTAC               GT^[AC][GT]AC
              763  GTATAC              
AccII          65  CGCG                 CG^CG
              188  CGCG                
BanII         244  GAGCTC               G[GA]GC[CT]^C
              967  GGGCTC              
BbvI           99  GCTGC                GCAGC(8/12)
             1020  GCTGC               
BglI          643  GCCAAGCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              821  GCCCAGCGGGC         
BsmFI         632  GGGAC                GGGAC(10/14)
              794  GGGAC               
BspMI         209  ACCTGC               ACCTGC(4/8)
              746  ACCTGC              
BsrDI         933  CATTGC               GCAATG(2/0)
              554  GCAATG              
BstUI          65  CGCG                 CG^CG
              188  CGCG                
BstXI         401  CCATCAACGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              884  CCATCCTTATGG        
DpnII         135  GATC                 ^GATC
              535  GATC                
DraII         206  AGGACCT              [GA]G^G[ACGT]CC[CT]
              334  AGGCCCC             
DsaI          441  CCGTGG               C^C[GA][CT]GG
              867  CCGTGG              
EaeI           46  TGGCCA               [CT]^GGCC[GA]
              870  TGGCCG              
EcoO109I      206  AGGACCT              [GA]G^G[ACGT]CC[CT]
              334  AGGCCCC             
HinfI         940  GACTC                G^A[ACGT]TC
              982  GAGTC               
MaeII         407  ACGT                 A^CGT
              755  ACGT                
MaeIII        895  GTGAC                ^GT[ACGT]AC
              984  GTCAC               
MboI          135  GATC                 ^GATC
              535  GATC                
MboII         151  GAAGA                GAAGA(8/7)
              296  TCTTC               
MseI          239  TTAA                 T^TAA
              950  TTAA                
MvnI           65  CGCG                 CG^CG
              188  CGCG                
NdeII         135  GATC                 ^GATC
              535  GATC                
NspI           27  GCATGT               [GA]CATG^[CT]
              173  GCATGC              
PleI          940  GACTC                GAGTC(4/5)
              982  GAGTC               
Sau3AI        135  GATC                 ^GATC
              535  GATC                
SmlI          247  CTCAAG               C^T[CT][GA]AG
              787  CTCGAG              
Tru9I         239  TTAA                 T^TAA
              950  TTAA                
TseI           99  GCTGC                G^C[AT]GC
             1020  GCTGC               
Tsp45I        895  GTGAC                ^GT[GC]AC
              984  GTCAC               
TspRI         427  CACTG                CA[GC]TG[ACGT][ACGT]^
             1034  CAGTG               
XcmI           34  CCAGAGACACGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              546  CCATCAGGGCAATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   44 enzymes) #
#########################################

AciI        AfaI        AluI        AspHI       AvaII       
BanI        BmyI        BpmI        BsaJI       BsiHKAI     
BsiYI       BslI        BsmAI       Bsp1286I    BssKI       
BstF5I      BstNI       Cac8I       CfoI        Cfr13I      
DdeI        EcoRII      FauI        Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HpaII       
HphI        ItaI        MnlI        MslI        MspA1I      
MspI        MvaI        MwoI        NlaIII      NlaIV       
RsaI        Sau96I      ScrFI       SfaNI       
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