################################## # NO CUT ENZYMES ( 171 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BlnI BpmI BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstF5I BstPI BstUI BstXI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraI DraII DraIII DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoT14I EcoT22I FauI FbaI FokI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MaeIII MamI MfeI MluI MroI MseI MunI MvnI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tru9I Tsp45I Tth111I TthHB8I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AcsI 50 AAATTT [GA]^AATT[CT] AlwI 597 GGATC GGATC(4/5) AlwNI 559 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 50 AAATTT [GA]^AATT[CT] AspHI 150 GAGCAC G[AT]GC[AT]^C AvaI 326 CCCGAG C^[CT]CG[GA]G AvaII 69 GGTCC G^G[AT]CC BalI 410 TGGCCA TGG^CCA BanI 274 GGTGCC G^G[CT][GA]CC BbvI 283 GCTGC GCAGC(8/12) BcnI 588 CCGGG CC^[GC]GG BglI 24 GCCCAATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 215 AGATCT A^GATCT BlpI 251 GCTTAGC GC^T[ACGT]AGC Bpu1102I 251 GCTTAGC GC^T[ACGT]AGC BsiHKAI 150 GAGCAC G[AT]GC[AT]^C BsmAI 481 GAGAC GTCTC(1/5) BsoBI 326 CCCGAG C^[CT]CG[GA]G BsrBI 161 GAGCGG CCGCTC(-3/-3) BstAPI 47 GCAAAATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 251 GCTTAGC GC^T[ACGT]AGC DrdI 359 GACAGGCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 410 TGGCCA [CT]^GGCC[GA] EcoRV 242 GATATC GAT^ATC HapII 588 CCGG C^CGG HpaII 588 CCGG C^CGG MluNI 410 TGGCCA TGG^CCA MscI 410 TGGCCA TGG^CCA MslI 198 CACAACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 227 CAGCTG C[AC]G^C[GT]G MspI 588 CCGG C^CGG NciI 588 CCGGG CC^[GC]GG NdeI 449 CATATG CA^TATG NlaIV 274 GGTGCC GG[ACGT]^[ACGT]CC PflMI 558 CCAGAATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 86 CTGCAG CTGCA^G PvuII 227 CAGCTG CAG^CTG SfaNI 374 GCATC GCATC(5/9) TseI 283 GCTGC G^C[AT]GC Van91I 558 CCAGAATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 189 TCTAGA T^CTAGA XcmI 413 CCAACAAACGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AciI 163 GCGG CCGC(-3/-1) 574 GCGG AfaI 141 GTAC GT^AC 264 GTAC BmyI 150 GAGCAC G[AGT]GC[ACT]^C 275 GTGCCC BsmFI 221 GGGAC GGGAC(10/14) 612 GTCCC Bsp1286I 150 GAGCAC G[AGT]GC[ACT]^C 275 GTGCCC BstYI 215 AGATCT [GA]^GATC[CT] 597 GGATCT Cac8I 43 GCCAGC GC[ACGT]^[ACGT]GC 577 GCAGGC DdeI 252 CTTAG C^T[ACGT]AG 533 CTAAG EarI 517 CTCTTC CTCTTC(1/4) 436 GAAGAG HinfI 82 GAATC G^A[ACGT]TC 561 GAATC HphI 342 GGTGA GGTGA(8/7) 173 TCACC Ksp632I 517 CTCTTC CTCTTC(1/4) 436 GAAGAG MboII 436 GAAGA GAAGA(8/7) 518 TCTTC MflI 215 AGATCT [GA]^GATC[CT] 597 GGATCT RsaI 141 GTAC GT^AC 264 GTAC TfiI 82 GAATC G^A[AT]TC 561 GAATC Tsp509I 51 AATT ^AATT 65 AATT XhoII 215 AGATCT [GA]^GATC[CT] 597 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 27 enzymes) # ######################################### AluI BfaI BsaJI BsiYI BslI BsrI BssKI BstNI Cfr13I DpnII EcoRII Fnu4HI HaeIII ItaI MaeI MaeII MboI MnlI MvaI MwoI NdeII NlaIII Sau3AI Sau96I ScrFI SfcI TspRI