Restriction map for
pF1KB8329
(Circular) (Six-base) MAPSORT of: pF1KB8329.seq Check: 8426 from: 1 to: 3758
pF1KB8329 3758 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 796 796
Size: 3758
Acc65I G'GTAC_C
Cuts at: 774 774
Size: 3758
AclI AA'CG_TT
Cuts at: 752 3598 752
Size: 2846 912
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 578 1583 2015 2762 3030 578
Size: 1005 432 747 268 1306
Fragments arranged by size:
1306 1005 747 432 268
AflIII A'CryG_T
Cuts at: 1110 2214 3191 1110
Size: 1104 977 1677
Fragments arranged by size:
1677 1104 977
AgeI A'CCGG_T
Cuts at: 2146 2146
Size: 3758
AleI CACnn'nnGTG
Cuts at: 310 310
Size: 3758
AlwNI CAG_nnn'CTG
Cuts at: 672 1063 2630 672
Size: 391 1567 1800
Fragments arranged by size:
1800 1567 391
ApaLI G'TGCA_C
Cuts at: 2528 2528
Size: 3758
ApoI r'AATT_y
Cuts at: 158 762 3111 3367 158
Size: 604 2349 256 549
Fragments arranged by size:
2349 604 549 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3758
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3758
AvaI C'yCGr_G
Cuts at: 434 778 434
Size: 344 3414
BamHI G'GATC_C
Cuts at: 783 783
Size: 3758
BanI G'GyrC_C
Cuts at: 382 774 1188 1436 1471 382
Size: 392 414 248 35 2669
Fragments arranged by size:
2669 414 392 248 35
BanII G_rGCy'C
Cuts at: 772 1802 772
Size: 1030 2728
BbeI G_GCGC'C
Cuts at: 1192 1440 1192
Size: 248 3510
BciVI GTATCCnnnnn_n'
Cuts at: 1656 2417 3758 1656
Size: 761 1341 1656
Fragments arranged by size:
1656 1341 761
BclI T'GATC_A
Cuts at: 1278 1278
Size: 3758
BglI GCCn_nnn'nGGC
Cuts at: 138 138
Size: 3758
BglII A'GATC_T
Cuts at: 323 1273 3310 323
Size: 950 2037 771
Fragments arranged by size:
2037 950 771
BlpI GC'TnA_GC
Cuts at: 360 870 360
Size: 510 3248
Bme1580I G_kGCm'C
Cuts at: 387 1383 1476 2532 387
Size: 996 93 1056 1613
Fragments arranged by size:
1613 1056 996 93
BmrI ACTGGGnnnn_n'
Cuts at: 279 970 1051 1386 3530 279
Size: 691 81 335 2144 507
Fragments arranged by size:
2144 691 507 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2125 2125
Size: 3758
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 934 1813 2305 2603 934
Size: 879 492 298 2089
Fragments arranged by size:
2089 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3462 3462
Size: 3758
BsaAI yAC'GTr
Cuts at: 1111 1111
Size: 3758
BsaBI GATnn'nnATC
Cuts at: 6 1272 3309 6
Size: 1266 2037 455
Fragments arranged by size:
2037 1266 455
BsaHI Gr'CG_yC
Cuts at: 1189 1437 2139 3635 1189
Size: 248 702 1496 1312
Fragments arranged by size:
1496 1312 702 248
BsaWI w'CCGG_w
Cuts at: 947 1468 2146 2420 2567 2978 947
Size: 521 678 274 147 411 1727
Fragments arranged by size:
1727 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 183 337 722 1183 1941 2518 3014 183
Size: 154 385 461 758 577 496 927
Fragments arranged by size:
927 758 577 496 461 385 154
BsiEI CG_ry'CG
Cuts at: 104 1300 1346 2554 104
Size: 1196 46 1208 1308
Fragments arranged by size:
1308 1208 1196 46
BsiHKAI G_wGCw'C
Cuts at: 262 772 1550 1740 2532 262
Size: 510 778 190 792 1488
Fragments arranged by size:
1488 792 778 510 190
Bsp1286I G_dGCh'C
Cuts at: 262 387 772 1383 1476 1550 1740 1802 2532
Size: 125 385 611 93 74 190 62 730
Cuts at: 2532 262
Size: 1488
Fragments arranged by size:
1488 730 611 385 190 125 93 74
62
BspEI T'CCGG_A
Cuts at: 2978 2978
Size: 3758
BspHI T'CATG_A
Cuts at: 2934 2934
Size: 3758
BspMI ACCTGCnnnn'nnnn_
Cuts at: 808 1022 1324 1705 808
Size: 214 302 381 2861
Fragments arranged by size:
2861 381 302 214
BsrBI CCG'CTC
Cuts at: 50 271 2050 2104 3588 3753 50
Size: 221 1779 54 1484 165 55
Fragments arranged by size:
1779 1484 221 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1670 1670
Size: 3758
BsrFI r'CCGG_y
Cuts at: 1756 1937 2146 1756
Size: 181 209 3368
Fragments arranged by size:
3368 209 181
BssSI C'ACGA_G
Cuts at: 2029 2387 2029
Size: 358 3400
BstAPI GCAn_nnn'nTGC
Cuts at: 161 1135 161
Size: 974 2784
BstBI TT'CG_AA
Cuts at: 2119 2119
Size: 3758
BstYI r'GATC_y
Cuts at: 1 323 705 783 1250 1273 1608 1854 2866
Size: 322 382 78 467 23 335 246 1012
Cuts at: 2866 2952 2964 3310 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 382 346 335 322 246
86 78 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1708 1889 2052 1708
Size: 181 163 3414
Fragments arranged by size:
3414 181 163
BtsI GCAGTG_nn'
Cuts at: 203 1001 203
Size: 798 2960
DraI TTT'AAA
Cuts at: 757 757
Size: 3758
DrdI GACnn_nn'nnGTC
Cuts at: 473 1464 2322 473
Size: 991 858 1909
Fragments arranged by size:
1909 991 858
EaeI y'GGCC_r
Cuts at: 518 1343 1517 1908 1935 3280 518
Size: 825 174 391 27 1345 996
Fragments arranged by size:
1345 996 825 391 174 27
EagI C'GGCC_G
Cuts at: 1343 1343
Size: 3758
EarI CTCTTCn'nnn_
Cuts at: 539 631 1781 1991 539
Size: 92 1150 210 2306
Fragments arranged by size:
2306 1150 210 92
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1966 2276 2422 3568 1966
Size: 310 146 1146 2156
Fragments arranged by size:
2156 1146 310 146
EcoICRI GAG'CTC
Cuts at: 770 770
Size: 3758
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 578 1583 2015 2762 3030 578
Size: 1005 432 747 268 1306
Fragments arranged by size:
1306 1005 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 897 897
Size: 3758
EcoRI G'AATT_C
Cuts at: 762 762
Size: 3758
EcoRV GAT'ATC
Cuts at: 352 352
Size: 3758
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1777 1777
Size: 3758
FspI TGC'GCA
Cuts at: 1539 1539
Size: 3758
HaeII r_GCGC'y
Cuts at: 1192 1440 2462 3441 1192
Size: 248 1022 979 1509
Fragments arranged by size:
1509 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1600 1630 1632 1662 1600
Size: 30 2 30 3696
Fragments arranged by size:
3696 30 30 2
HincII GTy'rAC
Cuts at: 797 797
Size: 3758
KasI G'GCGC_C
Cuts at: 1188 1436 1188
Size: 248 3510
KpnI G_GTAC'C
Cuts at: 778 778
Size: 3758
MluI A'CGCG_T
Cuts at: 3191 3191
Size: 3758
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2429 2613 2429
Size: 184 3574
MscI TGG'CCA
Cuts at: 520 1519 520
Size: 999 2759
MslI CAynn'nnrTG
Cuts at: 310 1747 1874 310
Size: 1437 127 2194
Fragments arranged by size:
2194 1437 127
MspA1I CmG'CkG
Cuts at: 337 869 1183 1543 2556 2801 337
Size: 532 314 360 1013 245 1294
Fragments arranged by size:
1294 1013 532 360 314 245
NaeI GCC'GGC
Cuts at: 1939 1939
Size: 3758
NarI GG'CG_CC
Cuts at: 1189 1437 1189
Size: 248 3510
NdeI CA'TA_TG
Cuts at: 558 558
Size: 3758
NgoMIV G'CCGG_C
Cuts at: 1937 1937
Size: 3758
NspI r_CATG'y
Cuts at: 811 1311 2218 811
Size: 500 907 2351
Fragments arranged by size:
2351 907 500
PciI A'CATG_T
Cuts at: 2214 2214
Size: 3758
PflMI CCAn_nnn'nTGG
Cuts at: 672 672
Size: 3758
PmeI GTTT'AAAC
Cuts at: 757 757
Size: 3758
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2897 2929 2897
Size: 32 3726
PstI C_TGCA'G
Cuts at: 198 805 1490 198
Size: 607 685 2466
Fragments arranged by size:
2466 685 607
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3758
PvuII CAG'CTG
Cuts at: 337 1183 1543 337
Size: 846 360 2552
Fragments arranged by size:
2552 846 360
RsrII CG'GwC_CG
Cuts at: 1953 1953
Size: 3758
SacI G_AGCT'C
Cuts at: 772 772
Size: 3758
SalI G'TCGA_C
Cuts at: 795 795
Size: 3758
SapI GCTCTTCn'nnn_
Cuts at: 1781 1991 1781
Size: 210 3548
SbfI CC_TGCA'GG
Cuts at: 805 805
Size: 3758
ScaI AGT'ACT
Cuts at: 3275 3275
Size: 3758
SfcI C'TryA_G
Cuts at: 33 194 375 460 801 1486 2479 2670 33
Size: 161 181 85 341 685 993 191 1121
Fragments arranged by size:
1121 993 685 341 191 181 161 85
SfoI GGC'GCC
Cuts at: 1190 1438 1190
Size: 248 3510
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3758
SmaI CCC'GGG
Cuts at: 780 780
Size: 3758
SmlI C'TyrA_G
Cuts at: 913 1828 2320 2582 913
Size: 915 492 262 2089
Fragments arranged by size:
2089 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3758
SphI G_CATG'C
Cuts at: 811 1311 811
Size: 500 3258
StyI C'CwwG_G
Cuts at: 892 1246 892
Size: 354 3404
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 298 438 1341 1474 1933 2142 298
Size: 140 903 133 459 209 1914
Fragments arranged by size:
1914 903 459 209 140 133
TatI w'GTAC_w
Cuts at: 371 3273 371
Size: 2902 856
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3275 3307 3275
Size: 32 3726
Tth111I GACn'n_nGTC
Cuts at: 1555 1555
Size: 3758
XbaI T'CTAG_A
Cuts at: 297 789 297
Size: 492 3266
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 528 1253 528
Size: 725 3033
XmaI C'CCGG_G
Cuts at: 778 778
Size: 3758
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NdeI NgoMIV NspI PciI PflMI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI
BmtI BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI
ClaI CspCI DraIII EcoNI FseI FspAI HindIII HpaI
MfeI NcoI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI