################################## # NO CUT ENZYMES ( 154 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AcsI AfaI AflII AflIII AgeI AluI Alw44I AlwI AlwNI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspHI AviII AvrII BalI BamHI BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BlnI BsaAI BsaBI BsaWI BseAI BsgI BsiHKAI BsiWI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HapII HincII HindII HindIII HpaI HpaII KpnI KspI MaeI MamI MfeI MluI MluNI MroI MscI MseI MspI MunI MvnI NaeI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuII RcaI RsaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI Tru9I XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AatII 205 GACGTC GACGT^C AhdI 322 GACGAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 342 CCTCAGG CC^T[ACGT]AGG ApaI 430 GGGCCC GGGCC^C AspEI 322 GACGAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 118 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 233 CTCGAG C^[CT]CG[GA]G BbeI 22 GGCGCC GGCGC^C BbsI 142 GTCTTC GAAGAC(2/6) BglII 155 AGATCT A^GATCT BlpI 62 GCTCAGC GC^T[ACGT]AGC Bpu1102I 62 GCTCAGC GC^T[ACGT]AGC BpuAI 142 GTCTTC GAAGAC(2/6) BsaI 136 GAGACC GGTCTC(1/5) BsiEI 464 CGATCG CG[GA][CT]^CG BsmAI 136 GAGAC GTCTC(1/5) BsoBI 233 CTCGAG C^[CT]CG[GA]G BstYI 155 AGATCT [GA]^GATC[CT] Bsu36I 342 CCTCAGG CC^T[ACGT]AGG CelII 62 GCTCAGC GC^T[ACGT]AGC DraII 430 GGGCCCT [GA]G^G[ACGT]CC[CT] Eam1105I 322 GACGAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 501 GAAGAG CTCTTC(1/4) Eco81I 342 CCTCAGG CC^T[ACGT]AGG EcoNI 342 CCTCAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 430 GGGCCCT [GA]G^G[ACGT]CC[CT] HgaI 118 GACGC GACGC(5/10) HphI 92 TCACC GGTGA(8/7) KasI 22 GGCGCC G^GCGCC Ksp632I 501 GAAGAG CTCTTC(1/4) MaeII 206 ACGT A^CGT MaeIII 229 GTCAC ^GT[ACGT]AC MflI 155 AGATCT [GA]^GATC[CT] MslI 444 CACTGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 57 CCGCTG C[AC]G^C[GT]G NarI 22 GGCGCC GG^CGCC NspI 166 ACATGC [GA]CATG^[CT] PaeR7I 233 CTCGAG C^TCGAG PflFI 118 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 328 GAGTC GAGTC(4/5) PvuI 464 CGATCG CGAT^CG SapI 501 GAAGAGC GCTCTTC(1/4) SfaNI 13 GCATC GCATC(5/9) SfcI 333 CTACAG C^T[GA][CT]AG SfoI 22 GGCGCC GGC^GCC SmlI 233 CTCGAG C^T[CT][GA]AG StuI 71 AGGCCT AGG^CCT TfiI 314 GATTC G^A[AT]TC Tsp45I 229 GTCAC ^GT[GC]AC Tsp509I 221 AATT ^AATT Tth111I 118 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 443 CCACTGAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 233 CTCGAG C^TCGAG XhoII 155 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AcyI 22 GGCGCC G[GA]^CG[CT]C 205 GACGTC BanI 22 GGCGCC G^G[CT][GA]CC 298 GGCACC BanII 82 GAGCCC G[GA]GC[CT]^C 430 GGGCCC BpmI 79 CTGGAG CTGGAG(16/14) 267 CTGGAG BsaHI 22 GGCGCC G[GA]^CG[CT]C 205 GACGTC BseRI 340 CTCCTC GAGGAG(10/8) 325 GAGGAG FauI 56 CCCGC CCCGC(4/6) 384 CCCGC Hin1I 22 GGCGCC G[GA]^CG[CT]C 205 GACGTC HinfI 314 GATTC G^A[ACGT]TC 328 GAGTC NlaIII 167 CATG CATG^ 189 CATG PflMI 183 CCAGAACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 240 CCAGGATGTGG TaqI 234 TCGA T^CGA 320 TCGA TthHB8I 234 TCGA T^CGA 320 TCGA Van91I 183 CCAGAACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 240 CCAGGATGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AciI AvaII BbvI BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeII HaeIII HhaI HinP1I ItaI MboI MboII MnlI MvaI MwoI NdeII NlaIV Sau3AI Sau96I ScrFI TseI TspRI