Restriction map for pF1KB8315
##################################
# NO CUT ENZYMES (  151 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AviII       AvrII       BalI        
BamHI       BanI        BbeI        BbrPI       BbvCI       
BclI        BfrI        BglI        BlnI        BlpI        
Bpu1102I    BsaAI       BsaHI       BsaWI       BseAI       
BsgI        BsiWI       BsmBI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrDI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DdeI        DraI        DrdI        DsaI        
Eam1105I    Eco47III    Eco81I      EcoO65I     EcoRV       
EcoT14I     EcoT22I     FbaI        FseI        FspI        
HaeII       Hin1I       HincII      HindII      HindIII     
HpaI        KasI        KpnI        KspI        MaeII       
MfeI        MluI        MluNI       MroI        MscI        
MseI        MslI        MunI        MvnI        NarI        
NcoI        NdeI        NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsrII       SacII       SalI        
SapI        SbfI        ScaI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TfiI        Tru9I       
Tth111I     Van91I      XbaI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AcsI          192  GAATTC               [GA]^AATT[CT]
ApoI          192  GAATTC               [GA]^AATT[CT]
Asp700I       108  GAAGATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI          322  CCCGAG               C^[CT]CG[GA]G
BbsI          420  GTCTTC               GAAGAC(2/6)
BcgI           13  GCAGATCTTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI          24  GGATAC               GTATCC(6/5)
BcnI          468  CCGGG                CC^[GC]GG
BfaI          200  CTAG                 C^TAG
BpmI          516  CTCCAG               CTGGAG(16/14)
BpuAI         420  GTCTTC               GAAGAC(2/6)
BsaBI         406  GATGACGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          124  GAGACC               GGTCTC(1/5)
BsiEI         465  CGGCCG               CG[GA][CT]^CG
BsmAI         124  GAGAC                GTCTC(1/5)
BsoBI         322  CCCGAG               C^[CT]CG[GA]G
BsrBI         670  CCGCTC               CCGCTC(-3/-3)
BsrI          354  CCAGT                ACTGG(1/-1)
CfoI           48  GCGC                 GCG^C
DraII         665  AGGTCCC              [GA]G^G[ACGT]CC[CT]
DraIII        394  CACCAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI          465  CGGCCG               [CT]^GGCC[GA]
EagI          465  CGGCCG               C^GGCCG
EclXI         465  CGGCCG               C^GGCCG
Eco52I        465  CGGCCG               C^GGCCG
EcoNI          73  CCTGTAGTAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      665  AGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoRI         192  GAATTC               G^AATTC
HhaI           48  GCGC                 GCG^C
HinP1I         48  GCGC                 G^CGC
HinfI         325  GAGTC                G^A[ACGT]TC
MaeI          200  CTAG                 C^TAG
MaeIII        361  GTGAC                ^GT[ACGT]AC
MamI          406  GATGACGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I        682  CAGCTG               C[AC]G^C[GT]G
NaeI          331  GCCGGC               GCC^GGC
NciI          468  CCGGG                CC^[GC]GG
NgoMIV        331  GCCGGC               G^CCGGC
PleI          325  GAGTC                GAGTC(4/5)
PpuMI         665  AGGTCCC              [GA]G^G[AT]CC[CT]
PvuII         682  CAGCTG               CAG^CTG
SacI          522  GAGCTC               GAGCT^C
SexAI         395  ACCAGGT              A^CC[AT]GGT
SfcI           74  CTGTAG               C^T[GA][CT]AG
Tsp45I        361  GTGAC                ^GT[GC]AC
Tsp509I       193  AATT                 ^AATT
XcmI          518  CCAGGAGCTCCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          108  GAAGATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AlwI           82  GGATC                GGATC(4/5)
              657  GGATC               
AlwNI         374  CAGATGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              682  CAGCTGCTG           
AspHI         522  GAGCTC               G[AT]GC[AT]^C
              632  GTGCTC              
AvaII         531  GGACC                G^G[AT]CC
              666  GGTCC               
BanII         319  GAGCCC               G[GA]GC[CT]^C
              522  GAGCTC              
BglII          15  AGATCT               A^GATCT
              110  AGATCT              
BsiHKAI       522  GAGCTC               G[AT]GC[AT]^C
              632  GTGCTC              
BsmFI         470  GGGAC                GGGAC(10/14)
              667  GTCCC               
BsrFI          50  GCCGGT               [GA]^CCGG[CT]
              331  GCCGGC              
BstYI          15  AGATCT               [GA]^GATC[CT]
              110  AGATCT              
Cfr10I         50  GCCGGT               [GA]^CCGG[CT]
              331  GCCGGC              
HgaI          241  GACGC                GACGC(5/10)
              382  GACGC               
MflI           15  AGATCT               [GA]^GATC[CT]
              110  AGATCT              
TaqI          116  TCGA                 T^CGA
              196  TCGA                
TspRI         359  CAGTG                CA[GC]TG[ACGT][ACGT]^
              474  CAGTG               
TthHB8I       116  TCGA                 T^CGA
              196  TCGA                
XhoII          15  AGATCT               [GA]^GATC[CT]
              110  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   42 enzymes) #
#########################################

AciI        AfaI        AluI        BbvI        BmyI        
BsaJI       BseRI       BsiYI       BslI        Bsp1286I    
BssKI       BstF5I      BstNI       Cac8I       Cfr13I      
DpnII       EarI        EcoRII      FauI        Fnu4HI      
FokI        HaeIII      HapII       HpaII       HphI        
ItaI        Ksp632I     MboI        MboII       MnlI        
MspI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       TseI        
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