Restriction map for pF1KB8314
 (Circular) (Six-base) MAPSORT of: pF1KB8314.seq  Check: 4648  from: 1  to: 4217



pF1KB8314  4217 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:28  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    326     326 
   Size:       4217
 
AccI GT'mk_AC

Cuts at:   1255    1255 
   Size:       4217
 
Acc65I G'GTAC_C

Cuts at:   1233    1233 
   Size:       4217
 
AclI AA'CG_TT

Cuts at:   4057    4057 
   Size:       4217
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    370     492     751    2042    2474    3221    3489     370 
   Size:        122     259    1291     432     747     268    1098

  Fragments arranged by size: 

                1291    1098     747     432     268     259     122
 
AflIII A'CryG_T

Cuts at:   1569    2673    3650    1569 
   Size:       1104     977    2136

  Fragments arranged by size: 

                2136    1104     977
 
AgeI A'CCGG_T

Cuts at:   2605    2605 
   Size:       4217
 
AleI CACnn'nnGTG

Cuts at:   1048    1048 
   Size:       4217
 
AlwNI CAG_nnn'CTG

Cuts at:    156    1522    3089     156 
   Size:       1366    1567    1284

  Fragments arranged by size: 

                1567    1366    1284
 
ApaLI G'TGCA_C

Cuts at:   2987    2987 
   Size:       4217
 
ApoI r'AATT_y

Cuts at:   1221    3570    3826    1221 
   Size:       2349     256    1612

  Fragments arranged by size: 

                2349    1612     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4217
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4217
 
AvaI C'yCGr_G

Cuts at:   1237    1237 
   Size:       4217
 
AvrII C'CTAG_G

Cuts at:    885     885 
   Size:       4217
 
BamHI G'GATC_C

Cuts at:   1242    1242 
   Size:       4217
 
BanI G'GyrC_C

Cuts at:    122     627     981    1233    1647    1895    1930     122 
   Size:        505     354     252     414     248      35    2409

  Fragments arranged by size: 

                2409     505     414     354     252     248      35
 
BanII G_rGCy'C

Cuts at:    422     599     653     953     995    1231    2261     422 
   Size:        177      54     300      42     236    1030    2378

  Fragments arranged by size: 

                2378    1030     300     236     177      54      42
 
BbeI G_GCGC'C

Cuts at:    126    1651    1899     126 
   Size:       1525     248    2444

  Fragments arranged by size: 

                2444    1525     248
 
BciVI GTATCCnnnnn_n'

Cuts at:   2115    2876    4217    2115 
   Size:        761    1341    2115

  Fragments arranged by size: 

                2115    1341     761
 
BclI T'GATC_A

Cuts at:   1737    1737 
   Size:       4217
 
BglI GCCn_nnn'nGGC

Cuts at:    120     990     120 
   Size:        870    3347
 
BglII A'GATC_T

Cuts at:    368     482    1732    3769     368 
   Size:        114    1250    2037     816

  Fragments arranged by size: 

                2037    1250     816     114
 
BlpI GC'TnA_GC

Cuts at:   1329    1329 
   Size:       4217
 
Bme1580I G_kGCm'C

Cuts at:    362     632     986    1136    1842    1935    2991     362 
   Size:        270     354     150     706      93    1056    1588

  Fragments arranged by size: 

                1588    1056     706     354     270     150      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1149    1429    1510    1845    3989    1149 
   Size:        280      81     335    2144    1377

  Fragments arranged by size: 

                2144    1377     335     280      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2584    2584 
   Size:       4217
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    430     712     734     865     997     430 
   Size:        282      22     131     132    3650

  Fragments arranged by size: 

                3650     282     132     131      22
 
Bpu10I CC'TnA_GC

Cuts at:    145     925     145 
   Size:        780    3437
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    988    1393    2272    2764    3062     988 
   Size:        405     879     492     298    2143

  Fragments arranged by size: 

                2143     879     492     405     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    448     787    3921     448 
   Size:        339    3134     744

  Fragments arranged by size: 

                3134     744     339
 
BsaAI yAC'GTr

Cuts at:   1570    1570 
   Size:       4217
 
BsaBI GATnn'nnATC

Cuts at:      6    1731    3768       6 
   Size:       1725    2037     455

  Fragments arranged by size: 

                2037    1725     455
 
BsaHI Gr'CG_yC

Cuts at:    123     251    1648    1896    2598    4094     123 
   Size:        128    1397     248     702    1496     246

  Fragments arranged by size: 

                1496    1397     702     248     246     128
 
BsaWI w'CCGG_w

Cuts at:    653    1406    1927    2605    2879    3026    3437     653 
   Size:        753     521     678     274     147     411    1433

  Fragments arranged by size: 

                1433     753     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    397     427     469     499     397 
   Size:         30      42      30    4115

  Fragments arranged by size: 

                4115      42      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1000    1000 
   Size:       4217
 
BseYI C'CCAG_C

Cuts at:    580     879    1642    2400    2977    3473     580 
   Size:        299     763     758     577     496    1324

  Fragments arranged by size: 

                1324     763     758     577     496     299
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1012    1168    1012 
   Size:        156    4061
 
BsiEI CG_ry'CG

Cuts at:    104    1759    1805    3013     104 
   Size:       1655      46    1208    1308

  Fragments arranged by size: 

                1655    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    337     422     599     953    1231    2009    2199    2991     337 
   Size:         85     177     354     278     778     190     792    1563

  Fragments arranged by size: 

                1563     792     778     354     278     190     177      85
            
 
Bsp1286I G_dGCh'C

Cuts at:    337     362     422     599     632     653     953     986     995 
   Size:         25      60     177      33      21     300      33       9

Cuts at:    995    1136    1231    1842    1935    2009    2199    2261    2991 
   Size:        141      95     611      93      74     190      62     730

Cuts at:   2991     337 
   Size:       1563

  Fragments arranged by size: 

                1563     730     611     300     190     177     141      95
                  93      74      62      60      33      33      25      21
                   9
 
BspEI T'CCGG_A

Cuts at:    653    3437     653 
   Size:       2784    1433
 
BspHI T'CATG_A

Cuts at:    209    3393     209 
   Size:       3184    1033
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    326     404     752     860    1267    1481    1783    2164     326 
   Size:         78     348     108     407     214     302     381    2379

  Fragments arranged by size: 

                2379     407     381     348     302     214     108      78
            
 
BsrBI CCG'CTC

Cuts at:     50    2509    2563    4047    4212      50 
   Size:       2459      54    1484     165      55

  Fragments arranged by size: 

                2459    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2129    2129 
   Size:       4217
 
BsrFI r'CCGG_y

Cuts at:   1152    2215    2396    2605    1152 
   Size:       1063     181     209    2764

  Fragments arranged by size: 

                2764    1063     209     181
 
BsrGI T'GTAC_A

Cuts at:    872     872 
   Size:       4217
 
BssSI C'ACGA_G

Cuts at:    127    2488    2846     127 
   Size:       2361     358    1498

  Fragments arranged by size: 

                2361    1498     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1594    1594 
   Size:       4217
 
BstBI TT'CG_AA

Cuts at:   2578    2578 
   Size:       4217
 
BstXI CCAn_nnnn'nTGG

Cuts at:    390     588     843     390 
   Size:        198     255    3764

  Fragments arranged by size: 

                3764     255     198
 
BstYI r'GATC_y

Cuts at:      1     368     482     491     563    1242    1709    1732    2067 
   Size:        367     114       9      72     679     467      23     335

Cuts at:   2067    2313    3325    3411    3423    3769       1 
   Size:        246    1012      86      12     346     449

  Fragments arranged by size: 

                1012     679     467     449     367     346     335     246
                 114      86      72      23      12       9
 
Bsu36I CC'TnA_GG

Cuts at:    246     987     246 
   Size:        741    3476
 
BtgI C'CryG_G

Cuts at:   1073    1073 
   Size:       4217
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2167    2348    2511    2167 
   Size:        181     163    3873

  Fragments arranged by size: 

                3873     181     163
 
BtsI GCAGTG_nn'

Cuts at:    309     514    1460     309 
   Size:        205     946    3066

  Fragments arranged by size: 

                3066     946     205
 
DraI TTT'AAA

Cuts at:   1216    1216 
   Size:       4217
 
DraIII CAC_nnn'GTG

Cuts at:    558     558 
   Size:       4217
 
DrdI GACnn_nn'nnGTC

Cuts at:   1923    2781    1923 
   Size:        858    3359
 
EaeI y'GGCC_r

Cuts at:    974    1154    1184    1802    1976    2367    2394    3739     974 
   Size:        180      30     618     174     391      27    1345    1452

  Fragments arranged by size: 

                1452    1345     618     391     180     174      30      27
            
 
EagI C'GGCC_G

Cuts at:   1802    1802 
   Size:       4217
 
EarI CTCTTCn'nnn_

Cuts at:   1123    2240    2450    1123 
   Size:       1117     210    2890

  Fragments arranged by size: 

                2890    1117     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2425    2735    2881    4027    2425 
   Size:        310     146    1146    2615

  Fragments arranged by size: 

                2615    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    420     597     951    1229     420 
   Size:        177     354     278    3408

  Fragments arranged by size: 

                3408     354     278     177
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    370     430     492     712     734     751     865     997    2042 
   Size:         60      62     220      22      17     114     132    1045

Cuts at:   2042    2474    3221    3489     370 
   Size:        432     747     268    1098

  Fragments arranged by size: 

                1098    1045     747     432     268     220     132     114
                  62      60      22      17
 
EcoNI CCTnn'n_nnAGG

Cuts at:    148     148 
   Size:       4217
 
EcoO109I rG'GnC_Cy

Cuts at:    153     849    1356     153 
   Size:        696     507    3014

  Fragments arranged by size: 

                3014     696     507
 
EcoRI G'AATT_C

Cuts at:   1221    1221 
   Size:       4217
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    450    2236     450 
   Size:       1786    2431
 
FspI TGC'GCA

Cuts at:    748    1998     748 
   Size:       1250    2967
 
HaeII r_GCGC'y

Cuts at:    126     239     290    1651    1899    2921    3900     126 
   Size:        113      51    1361     248    1022     979     443

  Fragments arranged by size: 

                1361    1022     979     443     248     113      51
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    250     282    2059    2089    2091    2121     250 
   Size:         32    1777      30       2      30    2346

  Fragments arranged by size: 

                2346    1777      32      30      30       2
 
HincII GTy'rAC

Cuts at:   1059    1256    1059 
   Size:        197    4020
 
HindIII A'AGCT_T

Cuts at:    227     227 
   Size:       4217
 
KasI G'GCGC_C

Cuts at:    122    1647    1895     122 
   Size:       1525     248    2444

  Fragments arranged by size: 

                2444    1525     248
 
KpnI G_GTAC'C

Cuts at:   1237    1237 
   Size:       4217
 
MluI A'CGCG_T

Cuts at:   3650    3650 
   Size:       4217
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    362    1058    2888    3072     362 
   Size:        696    1830     184    1507

  Fragments arranged by size: 

                1830    1507     696     184
 
MscI TGG'CCA

Cuts at:    976    1186    1978     976 
   Size:        210     792    3215

  Fragments arranged by size: 

                3215     792     210
 
MslI CAynn'nnrTG

Cuts at:    673    1048    2206    2333     673 
   Size:        375    1158     127    2557

  Fragments arranged by size: 

                2557    1158     375     127
 
MspA1I CmG'CkG

Cuts at:    647    1166    1328    1642    2002    3015    3260     647 
   Size:        519     162     314     360    1013     245    1604

  Fragments arranged by size: 

                1604    1013     519     360     314     245     162
 
NaeI GCC'GGC

Cuts at:   1154    2398    1154 
   Size:       1244    2973
 
NarI GG'CG_CC

Cuts at:    123    1648    1896     123 
   Size:       1525     248    2444

  Fragments arranged by size: 

                2444    1525     248
 
NcoI C'CATG_G

Cuts at:   1073    1073 
   Size:       4217
 
NgoMIV G'CCGG_C

Cuts at:   1152    2396    1152 
   Size:       1244    2973
 
NspI r_CATG'y

Cuts at:   1270    1770    2677    1270 
   Size:        500     907    2810

  Fragments arranged by size: 

                2810     907     500
 
PciI A'CATG_T

Cuts at:   2673    2673 
   Size:       4217
 
PflMI CCAn_nnn'nTGG

Cuts at:    558     558 
   Size:       4217
 
PfoI T'CCnGG_A

Cuts at:    254     944     254 
   Size:        690    3527
 
PmeI GTTT'AAAC

Cuts at:   1216    1216 
   Size:       4217
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3356    3388    3356 
   Size:         32    4185
 
PpuMI rG'GwC_Cy

Cuts at:    849     849 
   Size:       4217
 
PshAI GACnn'nnGTC

Cuts at:    344    1067     344 
   Size:        723    3494
 
PstI C_TGCA'G

Cuts at:    401     530    1264    1949     401 
   Size:        129     734     685    2669

  Fragments arranged by size: 

                2669     734     685     129
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4217
 
PvuII CAG'CTG

Cuts at:   1642    2002    1642 
   Size:        360    3857
 
RsrII CG'GwC_CG

Cuts at:   2412    2412 
   Size:       4217
 
SacI G_AGCT'C

Cuts at:    422     599     953    1231     422 
   Size:        177     354     278    3408

  Fragments arranged by size: 

                3408     354     278     177
 
SalI G'TCGA_C

Cuts at:   1254    1254 
   Size:       4217
 
SapI GCTCTTCn'nnn_

Cuts at:   2240    2450    2240 
   Size:        210    4007
 
SbfI CC_TGCA'GG

Cuts at:   1264    1264 
   Size:       4217
 
ScaI AGT'ACT

Cuts at:   3734    3734 
   Size:       4217
 
SexAI A'CCwGG_T

Cuts at:    554     554 
   Size:       4217
 
SfcI C'TryA_G

Cuts at:     33     282     397     526    1260    1945    2938    3129      33 
   Size:        249     115     129     734     685     993     191    1121

  Fragments arranged by size: 

                1121     993     734     685     249     191     129     115
            
 
SfoI GGC'GCC

Cuts at:    124    1649    1897     124 
   Size:       1525     248    2444

  Fragments arranged by size: 

                2444    1525     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4217
 
SmaI CCC'GGG

Cuts at:   1239    1239 
   Size:       4217
 
SmlI C'TyrA_G

Cuts at:   1003    1372    2287    2779    3041    1003 
   Size:        369     915     492     262    2179

  Fragments arranged by size: 

                2179     915     492     369     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4217
 
SphI G_CATG'C

Cuts at:   1270    1770    1270 
   Size:        500    3717
 
StuI AGG'CCT

Cuts at:    505     505 
   Size:       4217
 
StyI C'CwwG_G

Cuts at:    632     885    1073    1351    1705     632 
   Size:        253     188     278     354    3144

  Fragments arranged by size: 

                3144     354     278     253     188
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    291    1042    1800    1933    2392    2601     291 
   Size:        751     758     133     459     209    1907

  Fragments arranged by size: 

                1907     758     751     459     209     133
 
TatI w'GTAC_w

Cuts at:    872    3732     872 
   Size:       2860    1357
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3734    3766    3734 
   Size:         32    4185
 
Tth111I GACn'n_nGTC

Cuts at:    268    2014     268 
   Size:       1746    2471
 
XbaI T'CTAG_A

Cuts at:   1248    1248 
   Size:       4217
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    552     588    1712     552 
   Size:         36    1124    3057

  Fragments arranged by size: 

                3057    1124      36
 
XmaI C'CCGG_G

Cuts at:   1237    1237 
   Size:       4217
 
XmnI GAAnn'nnTTC

Cuts at:    505     505 
   Size:       4217
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AleI
    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    AvrII    BamHI
     BanI    BanII     BbeI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI    BssSI   BstAPI    BstBI    BstXI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII  HindIII     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI
     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI     PstI
     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI
    SexAI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     AlfI     AloI     ApaI     AscI
     BaeI     BbsI    BbvCI     BcgI    BfrBI    BmgBI     BmtI    BsiWI
     BsmI    BsmBI   BssHII   BstEII  BstZ17I     ClaI    CspCI    EcoRV
     FseI    FspAI     HpaI     MfeI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PasI     PmlI     PsiI   PspOMI    PspXI     PsrI
    SacII    SanDI     SfiI    SgrAI    SnaBI     SrfI     SspI     SwaI
     XhoI     ZraI
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