(Circular) (Six-base) MAPSORT of: pF1KB8314.seq Check: 4648 from: 1 to: 4217 pF1KB8314 4217 bp With 182 enzymes: SgfI * June 20, 2008 10:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 326 326 Size: 4217 AccI GT'mk_AC Cuts at: 1255 1255 Size: 4217 Acc65I G'GTAC_C Cuts at: 1233 1233 Size: 4217 AclI AA'CG_TT Cuts at: 4057 4057 Size: 4217 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 370 492 751 2042 2474 3221 3489 370 Size: 122 259 1291 432 747 268 1098 Fragments arranged by size: 1291 1098 747 432 268 259 122 AflIII A'CryG_T Cuts at: 1569 2673 3650 1569 Size: 1104 977 2136 Fragments arranged by size: 2136 1104 977 AgeI A'CCGG_T Cuts at: 2605 2605 Size: 4217 AleI CACnn'nnGTG Cuts at: 1048 1048 Size: 4217 AlwNI CAG_nnn'CTG Cuts at: 156 1522 3089 156 Size: 1366 1567 1284 Fragments arranged by size: 1567 1366 1284 ApaLI G'TGCA_C Cuts at: 2987 2987 Size: 4217 ApoI r'AATT_y Cuts at: 1221 3570 3826 1221 Size: 2349 256 1612 Fragments arranged by size: 2349 1612 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4217 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4217 AvaI C'yCGr_G Cuts at: 1237 1237 Size: 4217 AvrII C'CTAG_G Cuts at: 885 885 Size: 4217 BamHI G'GATC_C Cuts at: 1242 1242 Size: 4217 BanI G'GyrC_C Cuts at: 122 627 981 1233 1647 1895 1930 122 Size: 505 354 252 414 248 35 2409 Fragments arranged by size: 2409 505 414 354 252 248 35 BanII G_rGCy'C Cuts at: 422 599 653 953 995 1231 2261 422 Size: 177 54 300 42 236 1030 2378 Fragments arranged by size: 2378 1030 300 236 177 54 42 BbeI G_GCGC'C Cuts at: 126 1651 1899 126 Size: 1525 248 2444 Fragments arranged by size: 2444 1525 248 BciVI GTATCCnnnnn_n' Cuts at: 2115 2876 4217 2115 Size: 761 1341 2115 Fragments arranged by size: 2115 1341 761 BclI T'GATC_A Cuts at: 1737 1737 Size: 4217 BglI GCCn_nnn'nGGC Cuts at: 120 990 120 Size: 870 3347 BglII A'GATC_T Cuts at: 368 482 1732 3769 368 Size: 114 1250 2037 816 Fragments arranged by size: 2037 1250 816 114 BlpI GC'TnA_GC Cuts at: 1329 1329 Size: 4217 Bme1580I G_kGCm'C Cuts at: 362 632 986 1136 1842 1935 2991 362 Size: 270 354 150 706 93 1056 1588 Fragments arranged by size: 1588 1056 706 354 270 150 93 BmrI ACTGGGnnnn_n' Cuts at: 1149 1429 1510 1845 3989 1149 Size: 280 81 335 2144 1377 Fragments arranged by size: 2144 1377 335 280 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2584 2584 Size: 4217 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 430 712 734 865 997 430 Size: 282 22 131 132 3650 Fragments arranged by size: 3650 282 132 131 22 Bpu10I CC'TnA_GC Cuts at: 145 925 145 Size: 780 3437 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 988 1393 2272 2764 3062 988 Size: 405 879 492 298 2143 Fragments arranged by size: 2143 879 492 405 298 BsaI GGTCTCn'nnnn_ Cuts at: 448 787 3921 448 Size: 339 3134 744 Fragments arranged by size: 3134 744 339 BsaAI yAC'GTr Cuts at: 1570 1570 Size: 4217 BsaBI GATnn'nnATC Cuts at: 6 1731 3768 6 Size: 1725 2037 455 Fragments arranged by size: 2037 1725 455 BsaHI Gr'CG_yC Cuts at: 123 251 1648 1896 2598 4094 123 Size: 128 1397 248 702 1496 246 Fragments arranged by size: 1496 1397 702 248 246 128 BsaWI w'CCGG_w Cuts at: 653 1406 1927 2605 2879 3026 3437 653 Size: 753 521 678 274 147 411 1433 Fragments arranged by size: 1433 753 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 397 427 469 499 397 Size: 30 42 30 4115 Fragments arranged by size: 4115 42 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1000 1000 Size: 4217 BseYI C'CCAG_C Cuts at: 580 879 1642 2400 2977 3473 580 Size: 299 763 758 577 496 1324 Fragments arranged by size: 1324 763 758 577 496 299 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1012 1168 1012 Size: 156 4061 BsiEI CG_ry'CG Cuts at: 104 1759 1805 3013 104 Size: 1655 46 1208 1308 Fragments arranged by size: 1655 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 337 422 599 953 1231 2009 2199 2991 337 Size: 85 177 354 278 778 190 792 1563 Fragments arranged by size: 1563 792 778 354 278 190 177 85 Bsp1286I G_dGCh'C Cuts at: 337 362 422 599 632 653 953 986 995 Size: 25 60 177 33 21 300 33 9 Cuts at: 995 1136 1231 1842 1935 2009 2199 2261 2991 Size: 141 95 611 93 74 190 62 730 Cuts at: 2991 337 Size: 1563 Fragments arranged by size: 1563 730 611 300 190 177 141 95 93 74 62 60 33 33 25 21 9 BspEI T'CCGG_A Cuts at: 653 3437 653 Size: 2784 1433 BspHI T'CATG_A Cuts at: 209 3393 209 Size: 3184 1033 BspMI ACCTGCnnnn'nnnn_ Cuts at: 326 404 752 860 1267 1481 1783 2164 326 Size: 78 348 108 407 214 302 381 2379 Fragments arranged by size: 2379 407 381 348 302 214 108 78 BsrBI CCG'CTC Cuts at: 50 2509 2563 4047 4212 50 Size: 2459 54 1484 165 55 Fragments arranged by size: 2459 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 2129 2129 Size: 4217 BsrFI r'CCGG_y Cuts at: 1152 2215 2396 2605 1152 Size: 1063 181 209 2764 Fragments arranged by size: 2764 1063 209 181 BsrGI T'GTAC_A Cuts at: 872 872 Size: 4217 BssSI C'ACGA_G Cuts at: 127 2488 2846 127 Size: 2361 358 1498 Fragments arranged by size: 2361 1498 358 BstAPI GCAn_nnn'nTGC Cuts at: 1594 1594 Size: 4217 BstBI TT'CG_AA Cuts at: 2578 2578 Size: 4217 BstXI CCAn_nnnn'nTGG Cuts at: 390 588 843 390 Size: 198 255 3764 Fragments arranged by size: 3764 255 198 BstYI r'GATC_y Cuts at: 1 368 482 491 563 1242 1709 1732 2067 Size: 367 114 9 72 679 467 23 335 Cuts at: 2067 2313 3325 3411 3423 3769 1 Size: 246 1012 86 12 346 449 Fragments arranged by size: 1012 679 467 449 367 346 335 246 114 86 72 23 12 9 Bsu36I CC'TnA_GG Cuts at: 246 987 246 Size: 741 3476 BtgI C'CryG_G Cuts at: 1073 1073 Size: 4217 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2167 2348 2511 2167 Size: 181 163 3873 Fragments arranged by size: 3873 181 163 BtsI GCAGTG_nn' Cuts at: 309 514 1460 309 Size: 205 946 3066 Fragments arranged by size: 3066 946 205 DraI TTT'AAA Cuts at: 1216 1216 Size: 4217 DraIII CAC_nnn'GTG Cuts at: 558 558 Size: 4217 DrdI GACnn_nn'nnGTC Cuts at: 1923 2781 1923 Size: 858 3359 EaeI y'GGCC_r Cuts at: 974 1154 1184 1802 1976 2367 2394 3739 974 Size: 180 30 618 174 391 27 1345 1452 Fragments arranged by size: 1452 1345 618 391 180 174 30 27 EagI C'GGCC_G Cuts at: 1802 1802 Size: 4217 EarI CTCTTCn'nnn_ Cuts at: 1123 2240 2450 1123 Size: 1117 210 2890 Fragments arranged by size: 2890 1117 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2425 2735 2881 4027 2425 Size: 310 146 1146 2615 Fragments arranged by size: 2615 1146 310 146 EcoICRI GAG'CTC Cuts at: 420 597 951 1229 420 Size: 177 354 278 3408 Fragments arranged by size: 3408 354 278 177 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 370 430 492 712 734 751 865 997 2042 Size: 60 62 220 22 17 114 132 1045 Cuts at: 2042 2474 3221 3489 370 Size: 432 747 268 1098 Fragments arranged by size: 1098 1045 747 432 268 220 132 114 62 60 22 17 EcoNI CCTnn'n_nnAGG Cuts at: 148 148 Size: 4217 EcoO109I rG'GnC_Cy Cuts at: 153 849 1356 153 Size: 696 507 3014 Fragments arranged by size: 3014 696 507 EcoRI G'AATT_C Cuts at: 1221 1221 Size: 4217 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 450 2236 450 Size: 1786 2431 FspI TGC'GCA Cuts at: 748 1998 748 Size: 1250 2967 HaeII r_GCGC'y Cuts at: 126 239 290 1651 1899 2921 3900 126 Size: 113 51 1361 248 1022 979 443 Fragments arranged by size: 1361 1022 979 443 248 113 51 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 250 282 2059 2089 2091 2121 250 Size: 32 1777 30 2 30 2346 Fragments arranged by size: 2346 1777 32 30 30 2 HincII GTy'rAC Cuts at: 1059 1256 1059 Size: 197 4020 HindIII A'AGCT_T Cuts at: 227 227 Size: 4217 KasI G'GCGC_C Cuts at: 122 1647 1895 122 Size: 1525 248 2444 Fragments arranged by size: 2444 1525 248 KpnI G_GTAC'C Cuts at: 1237 1237 Size: 4217 MluI A'CGCG_T Cuts at: 3650 3650 Size: 4217 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 362 1058 2888 3072 362 Size: 696 1830 184 1507 Fragments arranged by size: 1830 1507 696 184 MscI TGG'CCA Cuts at: 976 1186 1978 976 Size: 210 792 3215 Fragments arranged by size: 3215 792 210 MslI CAynn'nnrTG Cuts at: 673 1048 2206 2333 673 Size: 375 1158 127 2557 Fragments arranged by size: 2557 1158 375 127 MspA1I CmG'CkG Cuts at: 647 1166 1328 1642 2002 3015 3260 647 Size: 519 162 314 360 1013 245 1604 Fragments arranged by size: 1604 1013 519 360 314 245 162 NaeI GCC'GGC Cuts at: 1154 2398 1154 Size: 1244 2973 NarI GG'CG_CC Cuts at: 123 1648 1896 123 Size: 1525 248 2444 Fragments arranged by size: 2444 1525 248 NcoI C'CATG_G Cuts at: 1073 1073 Size: 4217 NgoMIV G'CCGG_C Cuts at: 1152 2396 1152 Size: 1244 2973 NspI r_CATG'y Cuts at: 1270 1770 2677 1270 Size: 500 907 2810 Fragments arranged by size: 2810 907 500 PciI A'CATG_T Cuts at: 2673 2673 Size: 4217 PflMI CCAn_nnn'nTGG Cuts at: 558 558 Size: 4217 PfoI T'CCnGG_A Cuts at: 254 944 254 Size: 690 3527 PmeI GTTT'AAAC Cuts at: 1216 1216 Size: 4217 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3356 3388 3356 Size: 32 4185 PpuMI rG'GwC_Cy Cuts at: 849 849 Size: 4217 PshAI GACnn'nnGTC Cuts at: 344 1067 344 Size: 723 3494 PstI C_TGCA'G Cuts at: 401 530 1264 1949 401 Size: 129 734 685 2669 Fragments arranged by size: 2669 734 685 129 PvuI CG_AT'CG Cuts at: 104 104 Size: 4217 PvuII CAG'CTG Cuts at: 1642 2002 1642 Size: 360 3857 RsrII CG'GwC_CG Cuts at: 2412 2412 Size: 4217 SacI G_AGCT'C Cuts at: 422 599 953 1231 422 Size: 177 354 278 3408 Fragments arranged by size: 3408 354 278 177 SalI G'TCGA_C Cuts at: 1254 1254 Size: 4217 SapI GCTCTTCn'nnn_ Cuts at: 2240 2450 2240 Size: 210 4007 SbfI CC_TGCA'GG Cuts at: 1264 1264 Size: 4217 ScaI AGT'ACT Cuts at: 3734 3734 Size: 4217 SexAI A'CCwGG_T Cuts at: 554 554 Size: 4217 SfcI C'TryA_G Cuts at: 33 282 397 526 1260 1945 2938 3129 33 Size: 249 115 129 734 685 993 191 1121 Fragments arranged by size: 1121 993 734 685 249 191 129 115 SfoI GGC'GCC Cuts at: 124 1649 1897 124 Size: 1525 248 2444 Fragments arranged by size: 2444 1525 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4217 SmaI CCC'GGG Cuts at: 1239 1239 Size: 4217 SmlI C'TyrA_G Cuts at: 1003 1372 2287 2779 3041 1003 Size: 369 915 492 262 2179 Fragments arranged by size: 2179 915 492 369 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4217 SphI G_CATG'C Cuts at: 1270 1770 1270 Size: 500 3717 StuI AGG'CCT Cuts at: 505 505 Size: 4217 StyI C'CwwG_G Cuts at: 632 885 1073 1351 1705 632 Size: 253 188 278 354 3144 Fragments arranged by size: 3144 354 278 253 188 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 291 1042 1800 1933 2392 2601 291 Size: 751 758 133 459 209 1907 Fragments arranged by size: 1907 758 751 459 209 133 TatI w'GTAC_w Cuts at: 872 3732 872 Size: 2860 1357 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3734 3766 3734 Size: 32 4185 Tth111I GACn'n_nGTC Cuts at: 268 2014 268 Size: 1746 2471 XbaI T'CTAG_A Cuts at: 1248 1248 Size: 4217 XcmI CCAnnnn_n'nnnnTGG Cuts at: 552 588 1712 552 Size: 36 1124 3057 Fragments arranged by size: 3057 1124 36 XmaI C'CCGG_G Cuts at: 1237 1237 Size: 4217 XmnI GAAnn'nnTTC Cuts at: 505 505 Size: 4217 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AatII AfeI AflII AhdI AlfI AloI ApaI AscI BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI BsiWI BsmI BsmBI BssHII BstEII BstZ17I ClaI CspCI EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PmlI PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI