Restriction map for pF1KB8314
 (Linear) (Six-base) MAPSORT of: KIBB8314.seq  Check: 7817  from: 1  to: 1104



KIBB8314.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:04  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     218    1104 
   Size:        218     886
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     262     384     643    1104 
   Size:        262     122     259     461

  Fragments arranged by size: 

                 461     262     259     122
 
AleI CACnn'nnGTG

Cuts at:      0     940    1104 
   Size:        940     164
 
AlwNI CAG_nnn'CTG

Cuts at:      0      48    1104 
   Size:         48    1056
 
AvrII C'CTAG_G

Cuts at:      0     777    1104 
   Size:        777     327
 
BanI G'GyrC_C

Cuts at:      0      14     519     873    1104 
   Size:         14     505     354     231

  Fragments arranged by size: 

                 505     354     231      14
 
BanII G_rGCy'C

Cuts at:      0     314     491     545     845     887    1104 
   Size:        314     177      54     300      42     217

  Fragments arranged by size: 

                 314     300     217     177      54      42
 
BbeI G_GCGC'C

Cuts at:      0      18    1104 
   Size:         18    1086
 
BglI GCCn_nnn'nGGC

Cuts at:      0      12     882    1104 
   Size:         12     870     222

  Fragments arranged by size: 

                 870     222      12
 
BglII A'GATC_T

Cuts at:      0     260     374    1104 
   Size:        260     114     730

  Fragments arranged by size: 

                 730     260     114
 
Bme1580I G_kGCm'C

Cuts at:      0     254     524     878    1028    1104 
   Size:        254     270     354     150      76

  Fragments arranged by size: 

                 354     270     254     150      76
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1041    1104 
   Size:       1041      63
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     322     604     626     757     889    1104 
   Size:        322     282      22     131     132     215

  Fragments arranged by size: 

                 322     282     215     132     131      22
 
Bpu10I CC'TnA_GC

Cuts at:      0      37     817    1104 
   Size:         37     780     287

  Fragments arranged by size: 

                 780     287      37
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     880    1104 
   Size:        880     224
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     340     679    1104 
   Size:        340     339     425

  Fragments arranged by size: 

                 425     340     339
 
BsaHI Gr'CG_yC

Cuts at:      0      15     143    1104 
   Size:         15     128     961

  Fragments arranged by size: 

                 961     128      15
 
BsaWI w'CCGG_w

Cuts at:      0     545    1104 
   Size:        545     559
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     289     319     361     391    1104 
   Size:        289      30      42      30     713

  Fragments arranged by size: 

                 713     289      42      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     892    1104 
   Size:        892     212
 
BseYI C'CCAG_C

Cuts at:      0     472     771    1104 
   Size:        472     299     333

  Fragments arranged by size: 

                 472     333     299
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     904    1060    1104 
   Size:        904     156      44

  Fragments arranged by size: 

                 904     156      44
 
BsiHKAI G_wGCw'C

Cuts at:      0     229     314     491     845    1104 
   Size:        229      85     177     354     259

  Fragments arranged by size: 

                 354     259     229     177      85
 
Bsp1286I G_dGCh'C

Cuts at:      0     229     254     314     491     524     545     845     878 
   Size:        229      25      60     177      33      21     300      33

Cuts at:    878     887    1028    1104 
   Size:          9     141      76

  Fragments arranged by size: 

                 300     229     177     141      76      60      33      33
                  25      21       9
 
BspEI T'CCGG_A

Cuts at:      0     545    1104 
   Size:        545     559
 
BspHI T'CATG_A

Cuts at:      0     101    1104 
   Size:        101    1003
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     218     296     644     752    1104 
   Size:        218      78     348     108     352

  Fragments arranged by size: 

                 352     348     218     108      78
 
BsrFI r'CCGG_y

Cuts at:      0    1044    1104 
   Size:       1044      60
 
BsrGI T'GTAC_A

Cuts at:      0     764    1104 
   Size:        764     340
 
BssSI C'ACGA_G

Cuts at:      0      19    1104 
   Size:         19    1085
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     282     480     735    1104 
   Size:        282     198     255     369

  Fragments arranged by size: 

                 369     282     255     198
 
BstYI r'GATC_y

Cuts at:      0     260     374     383     455    1104 
   Size:        260     114       9      72     649

  Fragments arranged by size: 

                 649     260     114      72       9
 
Bsu36I CC'TnA_GG

Cuts at:      0     138     879    1104 
   Size:        138     741     225

  Fragments arranged by size: 

                 741     225     138
 
BtgI C'CryG_G

Cuts at:      0     965    1104 
   Size:        965     139
 
BtsI GCAGTG_nn'

Cuts at:      0     201     406    1104 
   Size:        201     205     698

  Fragments arranged by size: 

                 698     205     201
 
DraIII CAC_nnn'GTG

Cuts at:      0     450    1104 
   Size:        450     654
 
EaeI y'GGCC_r

Cuts at:      0     866    1046    1076    1104 
   Size:        866     180      30      28

  Fragments arranged by size: 

                 866     180      30      28
 
EarI CTCTTCn'nnn_

Cuts at:      0    1015    1104 
   Size:       1015      89
 
EcoICRI GAG'CTC

Cuts at:      0     312     489     843    1104 
   Size:        312     177     354     261

  Fragments arranged by size: 

                 354     312     261     177
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     262     322     384     604     626     643     757     889 
   Size:        262      60      62     220      22      17     114     132

Cuts at:    889    1104 
   Size:        215

  Fragments arranged by size: 

                 262     220     215     132     114      62      60      22
                  17
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      40    1104 
   Size:         40    1064
 
EcoO109I rG'GnC_Cy

Cuts at:      0      45     741    1104 
   Size:         45     696     363

  Fragments arranged by size: 

                 696     363      45
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     342    1104 
   Size:        342     762
 
FspI TGC'GCA

Cuts at:      0     640    1104 
   Size:        640     464
 
HaeII r_GCGC'y

Cuts at:      0      18     131     182    1104 
   Size:         18     113      51     922

  Fragments arranged by size: 

                 922     113      51      18
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     142     174    1104 
   Size:        142      32     930

  Fragments arranged by size: 

                 930     142      32
 
HincII GTy'rAC

Cuts at:      0     951    1104 
   Size:        951     153
 
HindIII A'AGCT_T

Cuts at:      0     119    1104 
   Size:        119     985
 
KasI G'GCGC_C

Cuts at:      0      14    1104 
   Size:         14    1090
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     254     950    1104 
   Size:        254     696     154

  Fragments arranged by size: 

                 696     254     154
 
MscI TGG'CCA

Cuts at:      0     868    1078    1104 
   Size:        868     210      26

  Fragments arranged by size: 

                 868     210      26
 
MslI CAynn'nnrTG

Cuts at:      0     565     940    1104 
   Size:        565     375     164

  Fragments arranged by size: 

                 565     375     164
 
MspA1I CmG'CkG

Cuts at:      0     539    1058    1104 
   Size:        539     519      46

  Fragments arranged by size: 

                 539     519      46
 
NaeI GCC'GGC

Cuts at:      0    1046    1104 
   Size:       1046      58
 
NarI GG'CG_CC

Cuts at:      0      15    1104 
   Size:         15    1089
 
NcoI C'CATG_G

Cuts at:      0     965    1104 
   Size:        965     139
 
NgoMIV G'CCGG_C

Cuts at:      0    1044    1104 
   Size:       1044      60
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     450    1104 
   Size:        450     654
 
PfoI T'CCnGG_A

Cuts at:      0     146     836    1104 
   Size:        146     690     268

  Fragments arranged by size: 

                 690     268     146
 
PpuMI rG'GwC_Cy

Cuts at:      0     741    1104 
   Size:        741     363
 
PshAI GACnn'nnGTC

Cuts at:      0     236     959    1104 
   Size:        236     723     145

  Fragments arranged by size: 

                 723     236     145
 
PstI C_TGCA'G

Cuts at:      0     293     422    1104 
   Size:        293     129     682

  Fragments arranged by size: 

                 682     293     129
 
SacI G_AGCT'C

Cuts at:      0     314     491     845    1104 
   Size:        314     177     354     259

  Fragments arranged by size: 

                 354     314     259     177
 
SexAI A'CCwGG_T

Cuts at:      0     446    1104 
   Size:        446     658
 
SfcI C'TryA_G

Cuts at:      0     174     289     418    1104 
   Size:        174     115     129     686

  Fragments arranged by size: 

                 686     174     129     115
 
SfoI GGC'GCC

Cuts at:      0      16    1104 
   Size:         16    1088
 
SmlI C'TyrA_G

Cuts at:      0     895    1104 
   Size:        895     209
 
StuI AGG'CCT

Cuts at:      0     397    1104 
   Size:        397     707
 
StyI C'CwwG_G

Cuts at:      0     524     777     965    1104 
   Size:        524     253     188     139

  Fragments arranged by size: 

                 524     253     188     139
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     183     934    1104 
   Size:        183     751     170

  Fragments arranged by size: 

                 751     183     170
 
TatI w'GTAC_w

Cuts at:      0     764    1104 
   Size:        764     340
 
Tth111I GACn'n_nGTC

Cuts at:      0     160    1104 
   Size:        160     944
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     444     480    1104 
   Size:        444      36     624

  Fragments arranged by size: 

                 624     444      36
 
XmnI GAAnn'nnTTC

Cuts at:      0     397    1104 
   Size:        397     707
 

 Enzymes that do cut:

     AarI     AcuI     AleI    AlwNI    AvrII     BanI    BanII     BbeI
     BglI    BglII Bme1580I     BmrI     BpmI   Bpu10I    BpuEI     BsaI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I
    BspEI    BspHI    BspMI    BsrFI    BsrGI    BssSI    BstXI    BstYI
   Bsu36I     BtgI     BtsI   DraIII     EaeI     EarI  EcoICRI  Eco57MI
    EcoNI EcoO109I     FalI     FspI    HaeII    Hin4I   HincII  HindIII
     KasI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV    PflMI     PfoI    PpuMI    PshAI     PstI     SacI    SexAI
     SfcI     SfoI     SmlI     StuI     StyI    TaqII     TatI  Tth111I
     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI     BaeI    BamHI     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BlpI    BmgBI     BmtI     BplI    BsaAI    BsaBI
    BsiEI    BsiWI     BsmI    BsmBI    BsrBI    BsrDI   BssHII   BstAPI
    BstBI   BstEII  BstZ17I    BtgZI     ClaI    CspCI     DraI     DrdI
     EagI     EciI    EcoRI    EcoRV     FseI    FspAI     HpaI     KpnI
     MfeI     MluI     NdeI     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI     PmeI     PmlI     PpiI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII    SacII     SalI    SanDI
     SapI     SbfI     ScaI     SfiI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI     TstI     XbaI     XhoI
     XmaI     ZraI
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