Restriction map for pF1KB8281
##################################
# NO CUT ENZYMES (  141 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        AlwNI       AocI        ApaI        ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AviII       BamHI       BanI        
BbeI        BbrPI       BbsI        BcgI        BciVI       
BclI        BfrI        BglII       BlpI        Bpu1102I    
BpuAI       BsaBI       BsaHI       BsaWI       BseAI       
BsgI        BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        Eam1105I    
Eco81I      EcoO65I     EcoRI       EcoRV       FbaI        
FseI        FspI        HgaI        Hin1I       HincII      
HindII      HindIII     HpaI        KasI        KpnI        
KspI        MamI        MfeI        MflI        MluI        
MroI        MspA1I      MunI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SbfI        ScaI        SfcI        SfiI        
SfoI        SfuI        SgrAI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tth111I     Van91I      XbaI        XhoI        XhoII       
XmnI        


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# ONE CUT ENZYMES (   36 enzymes) #
###################################

AccII         169  CGCG                 CG^CG
Alw44I        487  GTGCAC               G^TGCAC
Aor51HI       199  AGCGCT               AGC^GCT
ApaLI         487  GTGCAC               G^TGCAC
AspHI         487  GTGCAC               G[AT]GC[AT]^C
AvrII         727  CCTAGG               C^CTAGG
BalI          687  TGGCCA               TGG^CCA
BbvCI         299  CCTCAGC              CCTCAGC(-5/-2)
BlnI          727  CCTAGG               C^CTAGG
BsaAI         619  TACGTA               [CT]AC^GT[GA]
BsiEI         171  CGGCCG               CG[GA][CT]^CG
BsiHKAI       487  GTGCAC               G[AT]GC[AT]^C
BsiWI         617  CGTACG               C^GTACG
BstUI         169  CGCG                 CG^CG
EagI          171  CGGCCG               C^GGCCG
EclXI         171  CGGCCG               C^GGCCG
Eco47III      199  AGCGCT               AGC^GCT
Eco52I        171  CGGCCG               C^GGCCG
EcoNI         722  CCTGGCCTAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I       589  ATGCAT               ATGCA^T
MaeII         620  ACGT                 A^CGT
MaeIII        514  GTGAC                ^GT[ACGT]AC
MluNI         687  TGGCCA               TGG^CCA
MscI          687  TGGCCA               TGG^CCA
MseI          560  TTAA                 T^TAA
MvnI          169  CGCG                 CG^CG
NsiI          589  ATGCAT               ATGCA^T
NspI          572  ACATGC               [GA]CATG^[CT]
SexAI         223  ACCAGGT              A^CC[AT]GGT
SmaI          867  CCCGGG               CCC^GGG
SmlI          276  CTCAAG               C^T[CT][GA]AG
SnaBI         619  TACGTA               TAC^GTA
Tru9I         560  TTAA                 T^TAA
Tsp45I        514  GTGAC                ^GT[GC]AC
XmaCI         867  CCCGGG               C^CCGGG
XmaI          867  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AfaI          221  GTAC                 GT^AC
              618  GTAC                
AvaI          828  CCCGAG               C^[CT]CG[GA]G
              867  CCCGGG              
BanII          71  GGGCTC               G[GA]GC[CT]^C
              462  GAGCCC              
BsaI          227  GGTCTC               GGTCTC(1/5)
              794  GGTCTC              
BseRI         906  CTCCTC               GAGGAG(10/8)
              810  GAGGAG              
BsoBI         828  CCCGAG               C^[CT]CG[GA]G
              867  CCCGGG              
BspMI         791  GCAGGT               ACCTGC(4/8)
              836  GCAGGT              
BsrI          271  ACTGG                ACTGG(1/-1)
              206  CCAGT               
Cac8I          29  GCTTGC               GC[ACGT]^[ACGT]GC
              714  GCTGGC              
DraII         817  AGGTCCC              [GA]G^G[ACGT]CC[CT]
              838  AGGTCCC             
DsaI          578  CCGTGG               C^C[GA][CT]GG
              802  CCACGG              
EaeI          171  CGGCCG               [CT]^GGCC[GA]
              687  TGGCCA              
EcoO109I      817  AGGTCCC              [GA]G^G[ACGT]CC[CT]
              838  AGGTCCC             
HphI           79  TCACC                GGTGA(8/7)
              503  TCACC               
PpuMI         817  AGGTCCC              [GA]G^G[AT]CC[CT]
              838  AGGTCCC             
RsaI          221  GTAC                 GT^AC
              618  GTAC                
TaqI          154  TCGA                 T^CGA
              241  TCGA                
TfiI          633  GAATC                G^A[AT]TC
              746  GATTC               
Tsp509I       558  AATT                 ^AATT
              681  AATT                
TthHB8I       154  TCGA                 T^CGA
              241  TCGA                
XcmI          704  CCACCGCCGTGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              775  CCACCGCCTATGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   60 enzymes) #
#########################################

AciI        AluI        AlwI        AvaII       BbvI        
BcnI        BfaI        BglI        BmyI        BpmI        
BsaJI       BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BsrBI       BssKI       BstF5I      BstNI       
CfoI        Cfr13I      DdeI        DpnII       EarI        
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       ItaI        Ksp632I     MaeI        
MboI        MboII       MnlI        MslI        MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PleI        SapI        Sau3AI      Sau96I      
ScrFI       SfaNI       StyI        TseI        TspRI       
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