Restriction map for pF1KB8276
##################################
# NO CUT ENZYMES (  151 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AvaII       AvrII       BanI        BanII       BbeI        
BbrPI       BbvCI       BcgI        BciVI       BclI        
BfaI        BfrI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BsiHKAI     BsiWI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraI        DraII       
DrdI        Eam1105I    EarI        Eco47III    Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        HgaI        Hin1I       
HincII      HindII      HindIII     HpaI        KasI        
KpnI        Ksp632I     MaeI        MamI        MfeI        
MluI        MroI        MunI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        PvuII       RcaI        RsrII       SacI        
SalI        SapI        SbfI        ScaI        SfoI        
SfuI        SgrAI       SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
Tsp509I     Van91I      XbaI        XcmI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AspI          229  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         206  TGCGCA               TGC^GCA
BalI          251  TGGCCA               TGG^CCA
BamHI         356  GGATCC               G^GATCC
BbsI           21  GAAGAC               GAAGAC(2/6)
BglI          266  GCCGCGGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI          363  GGGCAC               G[AGT]GC[ACT]^C
BpuAI          21  GAAGAC               GAAGAC(2/6)
BseRI         241  GAGGAG               GAGGAG(10/8)
BsgI          132  CTGCAC               GTGCAG(16/14)
BsiEI         264  CGGCCG               CG[GA][CT]^CG
BsmAI          67  GAGAC                GTCTC(1/5)
BsmBI          67  GAGACG               CGTCTC(1/5)
Bsp1286I      363  GGGCAC               G[AGT]GC[ACT]^C
BsrI          101  ACTGG                ACTGG(1/-1)
BstXI          41  CCATGTTGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         356  GGATCC               [GA]^GATC[CT]
DdeI           29  CTCAG                C^T[ACGT]AG
DraIII         82  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI          264  CGGCCG               C^GGCCG
EclXI         264  CGGCCG               C^GGCCG
Eco52I        264  CGGCCG               C^GGCCG
EcoT14I       254  CCAAGG               C^C[AT][AT]GG
FauI          406  GCGGG                CCCGC(4/6)
FspI          206  TGCGCA               TGC^GCA
HaeII         316  GGCGCT               [GA]GCGC^[CT]
HinfI          33  GAATC                G^A[ACGT]TC
MaeIII         12  GTCAC                ^GT[ACGT]AC
MflI          356  GGATCC               [GA]^GATC[CT]
MluNI         251  TGGCCA               TGG^CCA
MscI          251  TGGCCA               TGG^CCA
MseI          113  TTAA                 T^TAA
NotI          263  GCGGCCGC             GC^GGCCGC
PflFI         229  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PstI          277  CTGCAG               CTGCA^G
SexAI          83  ACCTGGT              A^CC[AT]GGT
SfcI          277  CTGCAG               C^T[GA][CT]AG
SfiI          265  GGCCGCGGAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI          360  CCCGGG               CCC^GGG
StuI          273  AGGCCT               AGG^CCT
StyI          254  CCAAGG               C^C[AT][AT]GG
TaqI          227  TCGA                 T^CGA
TfiI           33  GAATC                G^A[AT]TC
Tru9I         113  TTAA                 T^TAA
Tsp45I         12  GTCAC                ^GT[GC]AC
TspRI          14  CACTG                CA[GC]TG[ACGT][ACGT]^
Tth111I       229  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       227  TCGA                 T^CGA
XhoII         356  GGATCC               [GA]^GATC[CT]
XmaCI         360  CCCGGG               C^CCGGG
XmaI          360  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AfaI           72  GTAC                 GT^AC
              201  GTAC                
AluI           53  AGCT                 AG^CT
               91  AGCT                
AlwI          357  GATCC                GGATC(4/5)
              356  GGATC               
AvaI          169  CCCGAG               C^[CT]CG[GA]G
              360  CCCGGG              
BsmFI         143  GGGAC                GGGAC(10/14)
              149  GGGAC               
BsoBI         169  CCCGAG               C^[CT]CG[GA]G
              360  CCCGGG              
BspMI         116  ACCTGC               ACCTGC(4/8)
              203  ACCTGC              
CfoI          207  GCGC                 GCG^C
              317  GCGC                
Cfr13I        258  GGGCC                G^G[ACGT]CC
              345  GGGCC               
DpnII         222  GATC                 ^GATC
              357  GATC                
HapII         342  CCGG                 C^CGG
              361  CCGG                
HhaI          207  GCGC                 GCG^C
              317  GCGC                
HinP1I        207  GCGC                 G^CGC
              317  GCGC                
HpaII         342  CCGG                 C^CGG
              361  CCGG                
HphI           87  GGTGA                GGTGA(8/7)
               36  TCACC               
MaeII          70  ACGT                 A^CGT
              136  ACGT                
MboI          222  GATC                 ^GATC
              357  GATC                
MboII          21  GAAGA                GAAGA(8/7)
              387  GAAGA               
MslI           42  CATGTTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              225  CATCGACATG          
MspI          342  CCGG                 C^CGG
              361  CCGG                
NdeII         222  GATC                 ^GATC
              357  GATC                
NlaIV         344  GGGGCC               GG[ACGT]^[ACGT]CC
              356  GGATCC              
RsaI           72  GTAC                 GT^AC
              201  GTAC                
Sau3AI        222  GATC                 ^GATC
              357  GATC                
Sau96I        258  GGGCC                G^G[ACGT]CC
              345  GGGCC               
SfaNI         165  GATGC                GCATC(5/9)
              209  GCATC               


#########################################
# 3 OR MORE CUT ENZYMES (   30 enzymes) #
#########################################

AccII       AciI        BbvI        BcnI        BsaJI       
BsiYI       BslI        BssKI       BstF5I      BstNI       
BstUI       Cac8I       DsaI        EaeI        EcoRII      
Fnu4HI      FokI        HaeIII      ItaI        KspI        
MnlI        MspA1I      MvaI        MvnI        MwoI        
NciI        NlaIII      SacII       ScrFI       TseI        
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