Restriction map for pF1KB8276
 (Linear) (Six-base) MAPSORT of: KIBB8276.seq  Check: 9580  from: 1  to: 417



KIBB8276.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:04  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40     417 
   Size:         40     377
 
AvaI C'yCGr_G

Cuts at:      0     169     360     417 
   Size:        169     191      57

  Fragments arranged by size: 

                 191     169      57
 
BamHI G'GATC_C

Cuts at:      0     356     417 
   Size:        356      61
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      28     417 
   Size:         28     389
 
BglI GCCn_nnn'nGGC

Cuts at:      0     272     417 
   Size:        272     145
 
Bme1580I G_kGCm'C

Cuts at:      0     367     417 
   Size:        367      50
 
BmgBI CAC'GTC

Cuts at:      0     137     417 
   Size:        137     280
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     256     417 
   Size:        256     161
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     117     417 
   Size:        117     300
 
BsiEI CG_ry'CG

Cuts at:      0     267     417 
   Size:        267     150
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      61     417 
   Size:         61     356
 
Bsp1286I G_dGCh'C

Cuts at:      0     367     417 
   Size:        367      50
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     125     212     417 
   Size:        125      87     205

  Fragments arranged by size: 

                 205     125      87
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      48     417 
   Size:         48     369
 
BstYI r'GATC_y

Cuts at:      0     356     417 
   Size:        356      61
 
BtgI C'CryG_G

Cuts at:      0     183     261     267     303     417 
   Size:        183      78       6      36     114

  Fragments arranged by size: 

                 183     114      78      36       6
 
BtsI GCAGTG_nn'

Cuts at:      0      13     417 
   Size:         13     404
 
DraIII CAC_nnn'GTG

Cuts at:      0      87     417 
   Size:         87     330
 
EaeI y'GGCC_r

Cuts at:      0     251     264     321     339     417 
   Size:        251      13      57      18      78

  Fragments arranged by size: 

                 251      78      57      18      13
 
EagI C'GGCC_G

Cuts at:      0     264     417 
   Size:        264     153
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     177     417 
   Size:        177     240
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40     417 
   Size:         40     377
 
FspI TGC'GCA

Cuts at:      0     208     417 
   Size:        208     209
 
HaeII r_GCGC'y

Cuts at:      0     320     417 
   Size:        320      97
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     113     145     341     373     417 
   Size:        113      32     196      32      44

  Fragments arranged by size: 

                 196     113      44      32      32
 
MscI TGG'CCA

Cuts at:      0     253     417 
   Size:        253     164
 
MslI CAynn'nnrTG

Cuts at:      0      46     229     417 
   Size:         46     183     188

  Fragments arranged by size: 

                 188     183      46
 
MspA1I CmG'CkG

Cuts at:      0     185     263     269     305     417 
   Size:        185      78       6      36     112

  Fragments arranged by size: 

                 185     112      78      36       6
 
NotI GC'GGCC_GC

Cuts at:      0     264     417 
   Size:        264     153
 
PstI C_TGCA'G

Cuts at:      0     281     417 
   Size:        281     136
 
SacII CC_GC'GG

Cuts at:      0     186     264     270     306     417 
   Size:        186      78       6      36     111

  Fragments arranged by size: 

                 186     111      78      36       6
 
SexAI A'CCwGG_T

Cuts at:      0      83     417 
   Size:         83     334
 
SfcI C'TryA_G

Cuts at:      0     277     417 
   Size:        277     140
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     272     417 
   Size:        272     145
 
SmaI CCC'GGG

Cuts at:      0     362     417 
   Size:        362      55
 
StuI AGG'CCT

Cuts at:      0     275     417 
   Size:        275     142
 
StyI C'CwwG_G

Cuts at:      0     254     417 
   Size:        254     163
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      71     103     417 
   Size:         71      32     314

  Fragments arranged by size: 

                 314      71      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     232     417 
   Size:        232     185
 
XmaI C'CCGG_G

Cuts at:      0     360     417 
   Size:        360      57
 

 Enzymes that do cut:

     AcuI     AvaI    BamHI     BbsI     BglI Bme1580I    BmgBI    BseRI
     BsgI    BsiEI    BsmBI Bsp1286I    BspMI    BstXI    BstYI     BtgI
     BtsI   DraIII     EaeI     EagI     EciI  Eco57MI     FspI    HaeII
    Hin4I     MscI     MslI   MspA1I     NotI     PstI    SacII    SexAI
     SfcI     SfiI     SmaI     StuI     StyI     TstI  Tth111I     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI     BanI    BanII
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseYI  BsiHKAI    BsiWI     BsmI
    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI
     DraI     DrdI     EarI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI    FspAI   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI     SalI    SanDI
     SapI     SbfI     ScaI     SfoI     SgfI    SgrAI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI    TaqII     TatI     XbaI
     XcmI     XhoI     XmnI     ZraI
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