Restriction map for
pF1KB8273
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# NO CUT ENZYMES ( 170 enzymes) #
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AatII Acc65I AccI AccII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbvCI BclI
BcnI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsiEI BsiWI BsmAI BsmBI
BsoBI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrBI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstYI BstZ17I Bsu36I CelII
CfoI ClaI CpoI DraI DraII
DraIII EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HhaI
Hin1I HinP1I HincII HindII HpaI
KasI KpnI Ksp632I KspI MaeII
MamI MfeI MflI MluI MluNI
MscI MseI MunI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI TaqI
Tru9I Tsp509I Tth111I TthHB8I XbaI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AfaI 732 GTAC GT^AC
AlwI 478 GGATC GGATC(4/5)
AspHI 319 GAGCTC G[AT]GC[AT]^C
BbsI 344 GTCTTC GAAGAC(2/6)
BcgI 128 GCACGACCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 97 GGATAC GTATCC(6/5)
BfaI 421 CTAG C^TAG
BpuAI 344 GTCTTC GAAGAC(2/6)
BsgI 413 GTGCAG GTGCAG(16/14)
BsiHKAI 319 GAGCTC G[AT]GC[AT]^C
BsmI 63 GAATGC GAATGC(1/-1)
BsrDI 529 GCAATG GCAATG(2/0)
BsrFI 728 GCCGGT [GA]^CCGG[CT]
BstXI 581 CCAAGAATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 610 GCTGGC GC[ACGT]^[ACGT]GC
Cfr10I 728 GCCGGT [GA]^CCGG[CT]
DdeI 767 CTGAG C^T[ACGT]AG
DpnII 479 GATC ^GATC
DrdI 649 GACCTCTGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 506 CCATGG C^C[GA][CT]GG
HgaI 656 GCGTC GACGC(5/10)
HindIII 103 AAGCTT A^AGCTT
HinfI 585 GAATC G^A[ACGT]TC
MaeI 421 CTAG C^TAG
MboI 479 GATC ^GATC
MslI 282 CACAGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 506 CCATGG C^CATGG
NdeII 479 GATC ^GATC
RsaI 732 GTAC GT^AC
SacI 319 GAGCTC GAGCT^C
Sau3AI 479 GATC ^GATC
SspI 247 AATATT AAT^ATT
TfiI 585 GAATC G^A[AT]TC
XcmI 134 CCATCGCTGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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AccIII 116 TCCGGA T^CCGGA
634 TCCGGA
BanII 319 GAGCTC G[GA]GC[CT]^C
630 GGGCTC
BpmI 316 CTGGAG CTGGAG(16/14)
784 CTGGAG
BsaWI 116 TCCGGA [AT]^CCGG[AT]
634 TCCGGA
BseAI 116 TCCGGA T^CCGGA
634 TCCGGA
BspEI 116 TCCGGA T^CCGGA
634 TCCGGA
EaeI 339 TGGCCG [CT]^GGCC[GA]
726 TGGCCG
EcoT14I 506 CCATGG C^C[AT][AT]GG
521 CCTTGG
MroI 116 TCCGGA T^CCGGA
634 TCCGGA
MspA1I 272 CAGCTG C[AC]G^C[GT]G
608 CAGCTG
PflMI 25 CCACCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
813 CCAAACAATGG
PstI 275 CTGCAG CTGCA^G
755 CTGCAG
PvuII 272 CAGCTG CAG^CTG
608 CAGCTG
StyI 506 CCATGG C^C[AT][AT]GG
521 CCTTGG
Tsp45I 358 GTGAC ^GT[GC]AC
502 GTCAC
Van91I 25 CCACCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
813 CCAAACAATGG
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AluI AvaII BbvI BmyI
BsaJI BseRI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Cfr13I EcoRII Fnu4HI FokI HaeIII
HapII HpaII HphI ItaI MaeIII
MboII MnlI MspI MvaI MwoI
NlaIII NlaIV Sau96I ScrFI SfaNI
SfcI TseI TspRI