Restriction map for pF1KB8273
 (Circular) (Six-base) MAPSORT of: pF1KB8273.seq  Check: 2214  from: 1  to: 3944



pF1KB8273  3944 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:28  ..

AccI GT'mk_AC

Cuts at:    982     982 
   Size:       3944
 
Acc65I G'GTAC_C

Cuts at:    960     960 
   Size:       3944
 
AclI AA'CG_TT

Cuts at:   3784    3784 
   Size:       3944
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    320     505    1769    2201    2948    3216     320 
   Size:        185    1264     432     747     268    1048

  Fragments arranged by size: 

                1264    1048     747     432     268     185
 
AflIII A'CryG_T

Cuts at:   1296    2400    3377    1296 
   Size:       1104     977    1863

  Fragments arranged by size: 

                1863    1104     977
 
AgeI A'CCGG_T

Cuts at:   2332    2332 
   Size:       3944
 
AlwNI CAG_nnn'CTG

Cuts at:   1249    2816    1249 
   Size:       1567    2377
 
ApaLI G'TGCA_C

Cuts at:   2714    2714 
   Size:       3944
 
ApoI r'AATT_y

Cuts at:    948    3297    3553     948 
   Size:       2349     256    1339

  Fragments arranged by size: 

                2349    1339     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3944
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3944
 
AvaI C'yCGr_G

Cuts at:    964     964 
   Size:       3944
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:    831     864     831 
   Size:         33    3911
 
BamHI G'GATC_C

Cuts at:    969     969 
   Size:       3944
 
BanI G'GyrC_C

Cuts at:    960    1374    1622    1657     960 
   Size:        414     248      35    3247

  Fragments arranged by size: 

                3247     414     248      35
 
BanII G_rGCy'C

Cuts at:    431     742     958    1988     431 
   Size:        311     216    1030    2387

  Fragments arranged by size: 

                2387    1030     311     216
 
BbeI G_GCGC'C

Cuts at:   1378    1626    1378 
   Size:        248    3696
 
BbsI GAAGACnn'nnnn_

Cuts at:    445     445 
   Size:       3944
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    225     259     225 
   Size:         34    3910
 
BciVI GTATCCnnnnn_n'

Cuts at:    199    1842    2603    3944     199 
   Size:       1643     761    1341     199

  Fragments arranged by size: 

                1643    1341     761     199
 
BclI T'GATC_A

Cuts at:   1464    1464 
   Size:       3944
 
BglI GCCn_nnn'nGGC

Cuts at:    112     112 
   Size:       3944
 
BglII A'GATC_T

Cuts at:   1459    3496    1459 
   Size:       2037    1907
 
BlpI GC'TnA_GC

Cuts at:   1056    1056 
   Size:       3944
 
Bme1580I G_kGCm'C

Cuts at:    812    1569    1662    2718     812 
   Size:        757      93    1056    2038

  Fragments arranged by size: 

                2038    1056     757      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1156    1237    1572    3716    1156 
   Size:         81     335    2144    1384

  Fragments arranged by size: 

                2144    1384     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    450    2311     450 
   Size:       1861    2083
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    445     913     445 
   Size:        468    3476
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1120    1999    2491    2789    1120 
   Size:        879     492     298    2275

  Fragments arranged by size: 

                2275     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3648    3648 
   Size:       3944
 
BsaAI yAC'GTr

Cuts at:   1297    1297 
   Size:       3944
 
BsaBI GATnn'nnATC

Cuts at:      6    1458    3495       6 
   Size:       1452    2037     455

  Fragments arranged by size: 

                2037    1452     455
 
BsaHI Gr'CG_yC

Cuts at:   1375    1623    2325    3821    1375 
   Size:        248     702    1496    1498

  Fragments arranged by size: 

                1498    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:    224     742    1133    1654    2332    2606    2753    3164     224 
   Size:        518     391     521     678     274     147     411    1004

  Fragments arranged by size: 

                1004     678     521     518     411     391     274     147
            
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    424     454     533     563     724     754     424 
   Size:         30      79      30     161      30    3614

  Fragments arranged by size: 

                3614     161      79      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    691     694     878     691 
   Size:          3     184    3757

  Fragments arranged by size: 

                3757     184       3
 
BseYI C'CCAG_C

Cuts at:    247     373     404    1369    2127    2704    3200     247 
   Size:        126      31     965     758     577     496     991

  Fragments arranged by size: 

                 991     965     758     577     496     126      31
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    542     542 
   Size:       3944
 
BsiEI CG_ry'CG

Cuts at:    104    1486    1532    2740     104 
   Size:       1382      46    1208    1308

  Fragments arranged by size: 

                1382    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    431     958    1736    1926    2718     431 
   Size:        527     778     190     792    1657

  Fragments arranged by size: 

                1657     792     778     527     190
 
BsmI GAATG_Cn'

Cuts at:    177     177 
   Size:       3944
 
Bsp1286I G_dGCh'C

Cuts at:    431     742     812     958    1569    1662    1736    1926    1988 
   Size:        311      70     146     611      93      74     190      62

Cuts at:   1988    2718     431 
   Size:        730    1657

  Fragments arranged by size: 

                1657     730     611     311     190     146      93      74
                  70      62
 
BspEI T'CCGG_A

Cuts at:    224     742    3164     224 
   Size:        518    2422    1004

  Fragments arranged by size: 

                2422    1004     518
 
BspHI T'CATG_A

Cuts at:   3120    3120 
   Size:       3944
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    994    1208    1510    1891     994 
   Size:        214     302     381    3047

  Fragments arranged by size: 

                3047     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2236    2290    3774    3939      50 
   Size:       2186      54    1484     165      55

  Fragments arranged by size: 

                2186    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    644    1856     644 
   Size:       1212    2732
 
BsrFI r'CCGG_y

Cuts at:    836    1942    2123    2332     836 
   Size:       1106     181     209    2448

  Fragments arranged by size: 

                2448    1106     209     181
 
BssSI C'ACGA_G

Cuts at:   2215    2573    2215 
   Size:        358    3586
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1321    1321 
   Size:       3944
 
BstBI TT'CG_AA

Cuts at:   2305    2305 
   Size:       3944
 
BstXI CCAn_nnnn'nTGG

Cuts at:    696     696 
   Size:       3944
 
BstYI r'GATC_y

Cuts at:      1     969    1436    1459    1794    2040    3052    3138    3150 
   Size:        968     467      23     335     246    1012      86      12

Cuts at:   3150    3496       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     968     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    107     614     107 
   Size:        507    3437
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    228     696    1894    2075    2238     228 
   Size:        468    1198     181     163    1934

  Fragments arranged by size: 

                1934    1198     468     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1187    1187 
   Size:       3944
 
DraI TTT'AAA

Cuts at:    943     943 
   Size:       3944
 
DrdI GACnn_nn'nnGTC

Cuts at:    763    1650    2508     763 
   Size:        887     858    2199

  Fragments arranged by size: 

                2199     887     858
 
EaeI y'GGCC_r

Cuts at:    447     834    1529    1703    2094    2121    3466     447 
   Size:        387     695     174     391      27    1345     925

  Fragments arranged by size: 

                1345     925     695     391     387     174      27
 
EagI C'GGCC_G

Cuts at:   1529    1529 
   Size:       3944
 
EarI CTCTTCn'nnn_

Cuts at:   1967    2177    1967 
   Size:        210    3734
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    784    2152    2462    2608    3754     784 
   Size:       1368     310     146    1146     974

  Fragments arranged by size: 

                1368    1146     974     310     146
 
EcoICRI GAG'CTC

Cuts at:    429     956     429 
   Size:        527    3417
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    320     445     505     913    1769    2201    2948    3216     320 
   Size:        125      60     408     856     432     747     268    1048

  Fragments arranged by size: 

                1048     856     747     432     408     268     125      60
            
 
EcoO109I rG'GnC_Cy

Cuts at:   1083    1083 
   Size:       3944
 
EcoRI G'AATT_C

Cuts at:    948     948 
   Size:       3944
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1963    1963 
   Size:       3944
 
FspI TGC'GCA

Cuts at:   1725    1725 
   Size:       3944
 
HaeII r_GCGC'y

Cuts at:   1378    1626    2648    3627    1378 
   Size:        248    1022     979    1695

  Fragments arranged by size: 

                1695    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    459     491     548     580     724     756    1786    1816    1818 
   Size:         32      57      32     144      32    1030      30       2

Cuts at:   1818    1848     459 
   Size:         30    2555

  Fragments arranged by size: 

                2555    1030     144      57      32      32      32      30
                  30       2
 
HincII GTy'rAC

Cuts at:    983     983 
   Size:       3944
 
HindIII A'AGCT_T

Cuts at:    211     211 
   Size:       3944
 
KasI G'GCGC_C

Cuts at:   1374    1622    1374 
   Size:        248    3696
 
KpnI G_GTAC'C

Cuts at:    964     964 
   Size:       3944
 
MluI A'CGCG_T

Cuts at:   3377    3377 
   Size:       3944
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    120     443    2615    2799     120 
   Size:        323    2172     184    1265

  Fragments arranged by size: 

                2172    1265     323     184
 
MscI TGG'CCA

Cuts at:   1705    1705 
   Size:       3944
 
MslI CAynn'nnrTG

Cuts at:    394    1933    2060     394 
   Size:       1539     127    2278

  Fragments arranged by size: 

                2278    1539     127
 
MspA1I CmG'CkG

Cuts at:    382     718    1055    1369    1729    2742    2987     382 
   Size:        336     337     314     360    1013     245    1339

  Fragments arranged by size: 

                1339    1013     360     337     336     314     245
 
NaeI GCC'GGC

Cuts at:   2125    2125 
   Size:       3944
 
NarI GG'CG_CC

Cuts at:   1375    1623    1375 
   Size:        248    3696
 
NcoI C'CATG_G

Cuts at:    107     614     107 
   Size:        507    3437
 
NgoMIV G'CCGG_C

Cuts at:   2123    2123 
   Size:       3944
 
NspI r_CATG'y

Cuts at:    997    1497    2404     997 
   Size:        500     907    2537

  Fragments arranged by size: 

                2537     907     500
 
PciI A'CATG_T

Cuts at:   2400    2400 
   Size:       3944
 
PflMI CCAn_nnn'nTGG

Cuts at:    139     927     139 
   Size:        788    3156
 
PmeI GTTT'AAAC

Cuts at:    943     943 
   Size:       3944
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3083    3115    3083 
   Size:         32    3912
 
PstI C_TGCA'G

Cuts at:    387     867     991    1676     387 
   Size:        480     124     685    2655

  Fragments arranged by size: 

                2655     685     480     124
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3944
 
PvuII CAG'CTG

Cuts at:    382     718    1369    1729     382 
   Size:        336     651     360    2597

  Fragments arranged by size: 

                2597     651     360     336
 
RsrII CG'GwC_CG

Cuts at:   2139    2139 
   Size:       3944
 
SacI G_AGCT'C

Cuts at:    431     958     431 
   Size:        527    3417
 
SalI G'TCGA_C

Cuts at:    981     981 
   Size:       3944
 
SapI GCTCTTCn'nnn_

Cuts at:   1967    2177    1967 
   Size:        210    3734
 
SbfI CC_TGCA'GG

Cuts at:    991     991 
   Size:       3944
 
ScaI AGT'ACT

Cuts at:   3461    3461 
   Size:       3944
 
SfcI C'TryA_G

Cuts at:     33     383     567     863     987    1672    2665    2856      33 
   Size:        350     184     296     124     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     350     296     191     184     124
            
 
SfoI GGC'GCC

Cuts at:   1376    1624    1376 
   Size:        248    3696
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3944
 
SmaI CCC'GGG

Cuts at:    966     966 
   Size:       3944
 
SmlI C'TyrA_G

Cuts at:   1099    2014    2506    2768    1099 
   Size:        915     492     262    2275

  Fragments arranged by size: 

                2275     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3944
 
SphI G_CATG'C

Cuts at:    997    1497     997 
   Size:        500    3444
 
SspI AAT'ATT

Cuts at:    357     357 
   Size:       3944
 
StyI C'CwwG_G

Cuts at:    107     614     629    1078    1432     107 
   Size:        507      15     449     354    2619

  Fragments arranged by size: 

                2619     507     449     354      15
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1527    1660    2119    2328    1527 
   Size:        133     459     209    3143

  Fragments arranged by size: 

                3143     459     209     133
 
TatI w'GTAC_w

Cuts at:   3459    3459 
   Size:       3944
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    424     456    3461    3493     424 
   Size:         32    3005      32     875

  Fragments arranged by size: 

                3005     875      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1741    1741 
   Size:       3944
 
XbaI T'CTAG_A

Cuts at:    975     975 
   Size:       3944
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    249    1439     249 
   Size:       1190    2754
 
XmaI C'CCGG_G

Cuts at:    964     964 
   Size:       3944
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI     BaeI    BamHI     BanI    BanII
     BbeI     BbsI     BcgI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BssSI   BstAPI    BstBI    BstXI    BstYI     BtgI    BtgZI     BtsI
     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
 EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII  HindIII
     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NspI     PciI    PflMI     PmeI     PpiI
     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI
     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI
     SspI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII    BbvCI    BfrBI    BmgBI     BmtI   Bpu10I
    BsiWI    BsmBI    BsrGI   BssHII   BstEII  BstZ17I   Bsu36I     ClaI
    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI     HpaI     MfeI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI     PfoI
     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI    SacII
    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     StuI     SwaI
     XhoI     XmnI     ZraI
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