Restriction map for pF1KB8273
 (Linear) (Six-base) MAPSORT of: KIBB8273.seq  Check: 3762  from: 1  to: 831



KIBB8273.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:04  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     212     397     831 
   Size:        212     185     434

  Fragments arranged by size: 

                 434     212     185
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     723     756     831 
   Size:        723      33      75

  Fragments arranged by size: 

                 723      75      33
 
BanII G_rGCy'C

Cuts at:      0     323     634     831 
   Size:        323     311     197

  Fragments arranged by size: 

                 323     311     197
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     337     831 
   Size:        337     494
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     117     151     831 
   Size:        117      34     680

  Fragments arranged by size: 

                 680     117      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      91     831 
   Size:         91     740
 
Bme1580I G_kGCm'C

Cuts at:      0     704     831 
   Size:        704     127
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     342     831 
   Size:        342     489
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     337     805     831 
   Size:        337     468      26

  Fragments arranged by size: 

                 468     337      26
 
BsaWI w'CCGG_w

Cuts at:      0     116     634     831 
   Size:        116     518     197

  Fragments arranged by size: 

                 518     197     116
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     316     346     425     455     616     646     831 
   Size:        316      30      79      30     161      30     185

  Fragments arranged by size: 

                 316     185     161      79      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     583     586     770     831 
   Size:        583       3     184      61

  Fragments arranged by size: 

                 583     184      61       3
 
BseYI C'CCAG_C

Cuts at:      0     139     265     296     831 
   Size:        139     126      31     535

  Fragments arranged by size: 

                 535     139     126      31
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     434     831 
   Size:        434     397
 
BsiHKAI G_wGCw'C

Cuts at:      0     323     831 
   Size:        323     508
 
BsmI GAATG_Cn'

Cuts at:      0      69     831 
   Size:         69     762
 
Bsp1286I G_dGCh'C

Cuts at:      0     323     634     704     831 
   Size:        323     311      70     127

  Fragments arranged by size: 

                 323     311     127      70
 
BspEI T'CCGG_A

Cuts at:      0     116     634     831 
   Size:        116     518     197

  Fragments arranged by size: 

                 518     197     116
 
BsrDI GCAATG_nn'

Cuts at:      0     536     831 
   Size:        536     295
 
BsrFI r'CCGG_y

Cuts at:      0     728     831 
   Size:        728     103
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     588     831 
   Size:        588     243
 
BtgI C'CryG_G

Cuts at:      0     506     831 
   Size:        506     325
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     120     588     831 
   Size:        120     468     243

  Fragments arranged by size: 

                 468     243     120
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     655     831 
   Size:        655     176
 
EaeI y'GGCC_r

Cuts at:      0     339     726     831 
   Size:        339     387     105

  Fragments arranged by size: 

                 387     339     105
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     676     831 
   Size:        676     155
 
EcoICRI GAG'CTC

Cuts at:      0     321     831 
   Size:        321     510
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     212     337     397     805     831 
   Size:        212     125      60     408      26

  Fragments arranged by size: 

                 408     212     125      60      26
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     351     383     440     472     616     648     831 
   Size:        351      32      57      32     144      32     183

  Fragments arranged by size: 

                 351     183     144      57      32      32      32
 
HindIII A'AGCT_T

Cuts at:      0     103     831 
   Size:        103     728
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      12     335     831 
   Size:         12     323     496

  Fragments arranged by size: 

                 496     323      12
 
MslI CAynn'nnrTG

Cuts at:      0     286     831 
   Size:        286     545
 
MspA1I CmG'CkG

Cuts at:      0     274     610     831 
   Size:        274     336     221

  Fragments arranged by size: 

                 336     274     221
 
NcoI C'CATG_G

Cuts at:      0     506     831 
   Size:        506     325
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      31     819     831 
   Size:         31     788      12

  Fragments arranged by size: 

                 788      31      12
 
PstI C_TGCA'G

Cuts at:      0     279     759     831 
   Size:        279     480      72

  Fragments arranged by size: 

                 480     279      72
 
PvuII CAG'CTG

Cuts at:      0     274     610     831 
   Size:        274     336     221

  Fragments arranged by size: 

                 336     274     221
 
SacI G_AGCT'C

Cuts at:      0     323     831 
   Size:        323     508
 
SfcI C'TryA_G

Cuts at:      0     275     459     755     831 
   Size:        275     184     296      76

  Fragments arranged by size: 

                 296     275     184      76
 
SspI AAT'ATT

Cuts at:      0     249     831 
   Size:        249     582
 
StyI C'CwwG_G

Cuts at:      0     506     521     831 
   Size:        506      15     310

  Fragments arranged by size: 

                 506     310      15
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     316     348     831 
   Size:        316      32     483

  Fragments arranged by size: 

                 483     316      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     141     831 
   Size:        141     690
 

 Enzymes that do cut:

     AcuI     BaeI    BanII     BbsI     BcgI    BciVI Bme1580I     BplI
     BpmI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BsrDI    BsrFI    BstXI     BtgI    BtgZI     DrdI
     EaeI     EciI  EcoICRI  Eco57MI    Hin4I  HindIII     MmeI     MslI
   MspA1I     NcoI    PflMI     PstI    PvuII     SacI     SfcI     SspI
     StyI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII    BamHI     BanI
     BbeI    BbvCI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmrI     BmtI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsiEI    BsiWI    BsmBI    BspHI    BspMI    BsrBI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I     BtsI
     ClaI    CspCI     DraI   DraIII     EagI     EarI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI
     SwaI    TaqII     TatI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI
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