Restriction map for pF1KB8269
##################################
# NO CUT ENZYMES (  158 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AlwNI       
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AvaI        
AviII       AvrII       BamHI       BbeI        BbvCI       
BcgI        BclI        BfaI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsgI        
BsiWI       BsmBI       BsmFI       BsmI        BsoBI       
BspDI       BspEI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrI        BssHII      BssSI       
Bst1107I    BstAPI      BstEII      BstPI       BstUI       
BstYI       BstZ17I     CelII       Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
Eam1105I    EarI        EclXI       Eco52I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT22I     FauI        
FbaI        FseI        FspI        HgaI        Hin1I       
HincII      HindII      HindIII     HpaI        HphI        
KasI        KpnI        Ksp632I     KspI        MaeI        
MaeIII      MamI        MfeI        MflI        MluI        
MroI        MspA1I      MunI        MvnI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        PacI        
PaeR7I      PflFI       PinAI       PmeI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RsrII       SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfcI        SfiI        
SfoI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    StuI        SwaI        
Tsp45I      Tth111I     XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AciI           62  CCGC                 CCGC(-3/-1)
AocI          477  CCTTAGG              CC^T[ACGT]AGG
Aor51HI       339  AGCGCT               AGC^GCT
AspHI         496  GAGCTC               G[AT]GC[AT]^C
AvaII         284  GGACC                G^G[AT]CC
BalI          371  TGGCCA               TGG^CCA
BanI          403  GGTGCC               G^G[CT][GA]CC
BbrPI         455  CACGTG               CAC^GTG
BbsI          272  GTCTTC               GAAGAC(2/6)
BcnI           54  CCGGG                CC^[GC]GG
BpuAI         272  GTCTTC               GAAGAC(2/6)
BsaAI         455  CACGTG               [CT]AC^GT[GA]
BsiHKAI       496  GAGCTC               G[AT]GC[AT]^C
Bsp1407I      159  TGTACA               T^GTACA
BspHI          93  TCATGA               T^CATGA
BsrGI         159  TGTACA               T^GTACA
BstBI         539  TTCGAA               TT^CGAA
BstXI         393  CCACGGCTATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        477  CCTTAGG              CC^T[ACGT]AGG
CfoI          340  GCGC                 GCG^C
DpnII          91  GATC                 ^GATC
Eco47III      339  AGCGCT               AGC^GCT
Eco81I        477  CCTTAGG              CC^T[ACGT]AGG
HaeII         339  AGCGCT               [GA]GCGC^[CT]
HapII          54  CCGG                 C^CGG
HhaI          340  GCGC                 GCG^C
HinP1I        340  GCGC                 G^CGC
HpaII          54  CCGG                 C^CGG
MboI           91  GATC                 ^GATC
MboII         273  TCTTC                GAAGA(8/7)
MluNI         371  TGGCCA               TGG^CCA
MscI          371  TGGCCA               TGG^CCA
MseI          107  TTAA                 T^TAA
MslI          394  CACGGCTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI           54  CCGG                 C^CGG
NciI           54  CCGGG                CC^[GC]GG
NdeII          91  GATC                 ^GATC
NspV          539  TTCGAA               TT^CGAA
PflMI         564  CCATGACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI         455  CACGTG               CAC^GTG
PmlI          455  CACGTG               CAC^GTG
RcaI           93  TCATGA               T^CATGA
SacI          496  GAGCTC               GAGCT^C
Sau3AI         91  GATC                 ^GATC
SfuI          539  TTCGAA               TT^CGAA
SspBI         159  TGTACA               T^GTACA
SspI           70  AATATT               AAT^ATT
Tru9I         107  TTAA                 T^TAA
Tsp509I       221  AATT                 ^AATT
Van91I        564  CCATGACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AfaI            6  GTAC                 GT^AC
              160  GTAC                
AluI          240  AGCT                 AG^CT
              497  AGCT                
Asp700I       172  GAATATATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              504  GAAACGCTTC          
BanII         496  GAGCTC               G[GA]GC[CT]^C
              597  GGGCTC              
BciVI          17  GTATCC               GTATCC(6/5)
              422  GTATCC              
BmyI          496  GAGCTC               G[AGT]GC[ACT]^C
              597  GGGCTC              
BseRI         304  CTCCTC               GAGGAG(10/8)
              493  GAGGAG              
BsiEI          51  CGACCG               CG[GA][CT]^CG
              429  CGACCG              
BsmAI         152  GAGAC                GTCTC(1/5)
              225  GTCTC               
Bsp1286I      496  GAGCTC               G[AGT]GC[ACT]^C
              597  GGGCTC              
BstF5I        513  CATCC                GGATG(2/0)
              561  CATCC               
DdeI          418  CTCAG                C^T[ACGT]AG
              478  CTTAG               
DraII          24  AGGCCCC              [GA]G^G[ACGT]CC[CT]
              377  AGGCCCC             
DsaI          230  CCACGG               C^C[GA][CT]GG
              393  CCACGG              
EaeI           59  TGGCCG               [CT]^GGCC[GA]
              371  TGGCCA              
EcoO109I       24  AGGCCCC              [GA]G^G[ACGT]CC[CT]
              377  AGGCCCC             
FokI          513  CATCC                GGATG(9/13)
              561  CATCC               
MaeII         188  ACGT                 A^CGT
              456  ACGT                
PleI          414  GACTC                GAGTC(4/5)
              279  GAGTC               
RsaI            6  GTAC                 GT^AC
              160  GTAC                
SfaNI         204  GATGC                GCATC(5/9)
              560  GCATC               
TaqI          428  TCGA                 T^CGA
              540  TCGA                
TfiI          519  GAATC                G^A[AT]TC
              542  GAATC               
TthHB8I       428  TCGA                 T^CGA
              540  TCGA                
XmnI          172  GAATATATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              504  GAAACGCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   25 enzymes) #
#########################################

BbvI        BpmI        BsaJI       BsiYI       BslI        
BssKI       BstNI       Cac8I       Cfr13I      EcoRII      
EcoT14I     Fnu4HI      HaeIII      HinfI       ItaI        
MnlI        MvaI        MwoI        NlaIII      NlaIV       
Sau96I      ScrFI       StyI        TseI        TspRI       
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