Restriction map for pF1KB8267
 (Linear) (Six-base) MAPSORT of: KIBB8267.seq  Check: 9946  from: 1  to: 693



KIBB8267.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:04  ..

AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      40      43     693 
   Size:         40       3     650

  Fragments arranged by size: 

                 650      40       3
 
AlwNI CAG_nnn'CTG

Cuts at:      0     457     466     693 
   Size:        457       9     227

  Fragments arranged by size: 

                 457     227       9
 
ApaI G_GGCC'C

Cuts at:      0     310     571     693 
   Size:        310     261     122

  Fragments arranged by size: 

                 310     261     122
 
ApoI r'AATT_y

Cuts at:      0     114     693 
   Size:        114     579
 
AvaI C'yCGr_G

Cuts at:      0     278     693 
   Size:        278     415
 
BamHI G'GATC_C

Cuts at:      0     611     693 
   Size:        611      82
 
BanI G'GyrC_C

Cuts at:      0      85     693 
   Size:         85     608
 
BanII G_rGCy'C

Cuts at:      0     310     492     571     693 
   Size:        310     182      79     122

  Fragments arranged by size: 

                 310     182     122      79
 
Bme1580I G_kGCm'C

Cuts at:      0     310     571     693 
   Size:        310     261     122

  Fragments arranged by size: 

                 310     261     122
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     492     693 
   Size:        492     201
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     314     539     693 
   Size:        314     225     154

  Fragments arranged by size: 

                 314     225     154
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     237     579     693 
   Size:        237     342     114

  Fragments arranged by size: 

                 342     237     114
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     245     557     656     693 
   Size:        245     312      99      37

  Fragments arranged by size: 

                 312     245      99      37
 
BseYI C'CCAG_C

Cuts at:      0     342     346     382     693 
   Size:        342       4      36     311

  Fragments arranged by size: 

                 342     311      36       4
 
BsiEI CG_ry'CG

Cuts at:      0      72     693 
   Size:         72     621
 
BsiHKAI G_wGCw'C

Cuts at:      0     549     693 
   Size:        549     144
 
BsmI GAATG_Cn'

Cuts at:      0     662     693 
   Size:        662      31
 
Bsp1286I G_dGCh'C

Cuts at:      0     310     492     549     571     693 
   Size:        310     182      57      22     122

  Fragments arranged by size: 

                 310     182     122      57      22
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     379     457     466     693 
   Size:        379      78       9     227

  Fragments arranged by size: 

                 379     227      78       9
 
BstYI r'GATC_y

Cuts at:      0      74     611     693 
   Size:         74     537      82

  Fragments arranged by size: 

                 537      82      74
 
BtgI C'CryG_G

Cuts at:      0     393     513     693 
   Size:        393     120     180

  Fragments arranged by size: 

                 393     180     120
 
EaeI y'GGCC_r

Cuts at:      0      69     510     693 
   Size:         69     441     183

  Fragments arranged by size: 

                 441     183      69
 
EagI C'GGCC_G

Cuts at:      0      69     693 
   Size:         69     624
 
EarI CTCTTCn'nnn_

Cuts at:      0     179     432     618     693 
   Size:        179     253     186      75

  Fragments arranged by size: 

                 253     186     179      75
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     314     539     693 
   Size:        314     225     154

  Fragments arranged by size: 

                 314     225     154
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     623     693 
   Size:        623      70
 
EcoO109I rG'GnC_Cy

Cuts at:      0     250     306     520     598     693 
   Size:        250      56     214      78      95

  Fragments arranged by size: 

                 250     214      95      78      56
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     517     693 
   Size:        517     176
 
MspA1I CmG'CkG

Cuts at:      0     346     457     463     693 
   Size:        346     111       6     230

  Fragments arranged by size: 

                 346     230     111       6
 
NspI r_CATG'y

Cuts at:      0     645     693 
   Size:        645      48
 
PasI CC'CwG_GG

Cuts at:      0     310     673     693 
   Size:        310     363      20

  Fragments arranged by size: 

                 363     310      20
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     292     394     577     693 
   Size:        292     102     183     116

  Fragments arranged by size: 

                 292     183     116     102
 
PfoI T'CCnGG_A

Cuts at:      0     651     693 
   Size:        651      42
 
PpuMI rG'GwC_Cy

Cuts at:      0     250     520     598     693 
   Size:        250     270      78      95

  Fragments arranged by size: 

                 270     250      95      78
 
PspOMI G'GGCC_C

Cuts at:      0     306     567     693 
   Size:        306     261     126

  Fragments arranged by size: 

                 306     261     126
 
PspXI vC'TCGA_Gb

Cuts at:      0     278     693 
   Size:        278     415
 
PstI C_TGCA'G

Cuts at:      0      32      65     375     462     693 
   Size:         32      33     310      87     231

  Fragments arranged by size: 

                 310     231      87      33      32
 
PvuII CAG'CTG

Cuts at:      0     346     463     693 
   Size:        346     117     230

  Fragments arranged by size: 

                 346     230     117
 
SanDI GG'GwC_CC

Cuts at:      0     598     693 
   Size:        598      95
 
SbfI CC_TGCA'GG

Cuts at:      0      65     375     693 
   Size:         65     310     318

  Fragments arranged by size: 

                 318     310      65
 
SfcI C'TryA_G

Cuts at:      0      28      61     371     458     693 
   Size:         28      33     310      87     235

  Fragments arranged by size: 

                 310     235      87      33      28
 
SmlI C'TyrA_G

Cuts at:      0     278     693 
   Size:        278     415
 
SphI G_CATG'C

Cuts at:      0     645     693 
   Size:        645      48
 
StuI AGG'CCT

Cuts at:      0     686     693 
   Size:        686       7
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     190     693 
   Size:        190     503
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     505     693 
   Size:        505     188
 
XhoI C'TCGA_G

Cuts at:      0     278     693 
   Size:        278     415
 

 Enzymes that do cut:

     AlfI    AlwNI     ApaI     ApoI     AvaI    BamHI     BanI    BanII
 Bme1580I     BmrI     BpmI     BsaI    BseRI    BseYI    BsiEI  BsiHKAI
     BsmI Bsp1286I   BstAPI    BstYI     BtgI     EaeI     EagI     EarI
  Eco57MI    EcoNI EcoO109I     MmeI   MspA1I     NspI     PasI    PflMI
     PfoI    PpuMI   PspOMI    PspXI     PstI    PvuII    SanDI     SbfI
     SfcI     SmlI     SphI     StuI    TaqII     XcmI     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AloI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI     BplI
   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI     BsgI
    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EciI
  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PciI     PmeI     PmlI     PpiI
    PshAI     PsiI     PsrI     PvuI    RsrII     SacI    SacII     SalI
     SapI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SrfI     SspI     StyI     SwaI     TatI     TstI
  Tth111I     XbaI     XmaI     XmnI     ZraI
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