Restriction map for pF1KB8264
##################################
# NO CUT ENZYMES (  146 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BalI        BanI        BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BcnI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsiWI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspLU11I    BsrBI       BsrDI       BsrGI       Bst1107I    
BstBI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DraI        DraIII      DrdI        Eam1105I    
EarI        Eco47III    Eco81I      EcoNI       EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HaeII       HgaI        Hin1I       HindIII     HinfI       
HpaI        KasI        KpnI        Ksp632I     MamI        
MfeI        MluI        MluNI       MroI        MscI        
MseI        MunI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SacI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        Sse8387I    SspBI       
SspI        StuI        SwaI        TfiI        Tru9I       
Tth111I     XbaI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AccI          355  GTCGAC               GT^[AC][GT]AC
BamHI         515  GGATCC               G^GATCC
BfaI          430  CTAG                 C^TAG
BspHI         449  TCATGA               T^CATGA
BsrI          587  CCAGT                ACTGG(1/-1)
BssHII        538  GCGCGC               G^CGCGC
BssSI         424  CACGAG               CACGAG(-5/-1)
BstEII         81  GGTGACC              G^GT[ACGT]ACC
BstPI          81  GGTGACC              G^GT[ACGT]ACC
BstXI          63  CCACGACCGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         515  GGATCC               [GA]^GATC[CT]
DdeI          437  CTGAG                C^T[ACGT]AG
DraII         527  GGGACCC              [GA]G^G[ACGT]CC[CT]
EaeI          154  CGGCCG               [CT]^GGCC[GA]
EagI          154  CGGCCG               C^GGCCG
EclXI         154  CGGCCG               C^GGCCG
Eco52I        154  CGGCCG               C^GGCCG
EcoO109I      527  GGGACCC              [GA]G^G[ACGT]CC[CT]
EcoO65I        81  GGTGACC              G^GT[ACGT]ACC
FauI          523  GCGGG                CCCGC(4/6)
HincII        355  GTCGAC               GT[CT]^[GA]AC
HindII        355  GTCGAC               GT[CT]^[GA]AC
KspI          157  CCGCGG               CCGC^GG
MaeI          430  CTAG                 C^TAG
MboII         573  GAAGA                GAAGA(8/7)
MflI          515  GGATCC               [GA]^GATC[CT]
NotI          153  GCGGCCGC             GC^GGCCGC
NspI          278  GCATGC               [GA]CATG^[CT]
PflMI         102  CCAGACCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         527  GGGACCC              [GA]G^G[AT]CC[CT]
PstI          391  CTGCAG               CTGCA^G
RcaI          449  TCATGA               T^CATGA
SacII         157  CCGCGG               CCGC^GG
SalI          355  GTCGAC               G^TCGAC
SfaNI         248  GCATC                GCATC(5/9)
SfcI          391  CTGCAG               C^T[GA][CT]AG
SphI          278  GCATGC               GCATG^C
TaqI          356  TCGA                 T^CGA
Tsp509I       128  AATT                 ^AATT
TspRI         588  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       356  TCGA                 T^CGA
Van91I        102  CCAGACCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         515  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AccII         158  CGCG                 CG^CG
              539  CGCG                
AlwI          516  GATCC                GGATC(4/5)
              515  GGATC               
AspHI         421  GAGCAC               G[AT]GC[AT]^C
              439  GAGCAC              
AvaII         168  GGACC                G^G[AT]CC
              528  GGACC               
BanII         235  GAGCCC               G[GA]GC[CT]^C
              302  GGGCTC              
BpmI          109  CTGGAG               CTGGAG(16/14)
              379  CTGGAG              
BsgI          316  GTGCAG               GTGCAG(16/14)
              625  GTGCAG              
BsiEI          66  CGACCG               CG[GA][CT]^CG
              154  CGGCCG              
BsiHKAI       421  GAGCAC               G[AT]GC[AT]^C
              439  GAGCAC              
BsmAI          16  GAGAC                GTCTC(1/5)
              592  GAGAC               
BsmBI          16  GAGACG               CGTCTC(1/5)
              592  GAGACG              
BspMI         564  GCAGGT               ACCTGC(4/8)
              602  GCAGGT              
BsrFI          23  ACCGGC               [GA]^CCGG[CT]
              617  ACCGGC              
BstAPI        384  GCAGTTTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              396  GCAGGAGCTGC         
BstF5I        375  CATCC                GGATG(2/0)
              579  CATCC               
BstUI         158  CGCG                 CG^CG
              539  CGCG                
Cfr10I         23  ACCGGC               [GA]^CCGG[CT]
              617  ACCGGC              
DpnII         516  GATC                 ^GATC
              576  GATC                
DsaI           69  CCGTGG               C^C[GA][CT]GG
              157  CCGCGG              
EcoT14I       239  CCAAGG               C^C[AT][AT]GG
              455  CCAAGG              
FokI          375  CATCC                GGATG(9/13)
              579  CATCC               
MaeII          19  ACGT                 A^CGT
              443  ACGT                
MaeIII         82  GTGAC                ^GT[ACGT]AC
              466  GTGAC               
MboI          516  GATC                 ^GATC
              576  GATC                
MvnI          158  CGCG                 CG^CG
              539  CGCG                
NdeII         516  GATC                 ^GATC
              576  GATC                
PvuII          55  CAGCTG               CAG^CTG
              505  CAGCTG              
Sau3AI        516  GATC                 ^GATC
              576  GATC                
StyI          239  CCAAGG               C^C[AT][AT]GG
              455  CCAAGG              
Tsp45I         82  GTGAC                ^GT[GC]AC
              466  GTGAC               
XcmI          341  CCAAGCAGTCCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              368  CCAAGTACATCCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   38 enzymes) #
#########################################

AciI        AfaI        AluI        AlwNI       BbvI        
BmyI        BsaJI       BseRI       BsiYI       BslI        
BsmFI       Bsp1286I    BssKI       BstNI       Cac8I       
CfoI        Cfr13I      EcoRII      Fnu4HI      HaeIII      
HapII       HhaI        HinP1I      HpaII       HphI        
ItaI        MnlI        MslI        MspA1I      MspI        
MvaI        MwoI        NlaIII      NlaIV       RsaI        
Sau96I      ScrFI       TseI        
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