Restriction map for pF1KB8247
##################################
# NO CUT ENZYMES (  154 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AviII       AvrII       
BanI        BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsgI        
BsiEI       BsiWI       BsmAI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraI        DrdI        
DsaI        EagI        Eam1105I    EclXI       Eco52I      
Eco81I      EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HgaI        Hin1I       
HindIII     HpaI        HphI        KasI        KpnI        
KspI        MaeII       MamI        MfeI        MluI        
MroI        MseI        MslI        MunI        MvnI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
PstI        PvuI        RcaI        RsrII       SacII       
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
TfiI        Tru9I       Tth111I     XbaI        XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AfaI            3  GTAC                 GT^AC
Aor51HI       215  AGCGCT               AGC^GCT
AspHI          94  GAGCTC               G[AT]GC[AT]^C
AvaI          268  CCCGAG               C^[CT]CG[GA]G
BamHI         587  GGATCC               G^GATCC
BbsI          697  GAAGAC               GAAGAC(2/6)
BfaI           82  CTAG                 C^TAG
BpuAI         697  GAAGAC               GAAGAC(2/6)
BseRI         307  CTCCTC               GAGGAG(10/8)
BsiHKAI        94  GAGCTC               G[AT]GC[AT]^C
BsoBI         268  CCCGAG               C^[CT]CG[GA]G
BsrBI         358  GAGCGG               CCGCTC(-3/-3)
BstXI         716  CCACAGAACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         587  GGATCC               [GA]^GATC[CT]
CfoI          216  GCGC                 GCG^C
DraIII        403  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco47III      215  AGCGCT               AGC^GCT
HaeII         215  AGCGCT               [GA]GCGC^[CT]
HhaI          216  GCGC                 GCG^C
HinP1I        216  GCGC                 G^CGC
HincII         71  GTCGAC               GT[CT]^[GA]AC
HindII         71  GTCGAC               GT[CT]^[GA]AC
MaeI           82  CTAG                 C^TAG
MflI          587  GGATCC               [GA]^GATC[CT]
MspA1I        754  CAGCTG               C[AC]G^C[GT]G
NlaIII        222  CATG                 CATG^
PflMI         288  CCAGCTCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         644  GGGTCCT              [GA]G^G[AT]CC[CT]
PshAI         384  GACCAATGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII         754  CAGCTG               CAG^CTG
RsaI            3  GTAC                 GT^AC
SacI           94  GAGCTC               GAGCT^C
SalI           71  GTCGAC               G^TCGAC
SapI           61  GAAGAGC              GCTCTTC(1/4)
SfcI          666  CTACAG               C^T[GA][CT]AG
StuI          551  AGGCCT               AGG^CCT
Tsp45I        155  GTGAC                ^GT[GC]AC
Tsp509I       144  AATT                 ^AATT
Van91I        288  CCAGCTCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         587  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AccI           71  GTCGAC               GT^[AC][GT]AC
              391  GTCTAC              
AlwI          588  GATCC                GGATC(4/5)
              587  GGATC               
ApaI          173  GGGCCC               GGGCC^C
              651  GGGCCC              
AvaII         119  GGACC                G^G[AT]CC
              645  GGTCC               
BalI          458  TGGCCA               TGG^CCA
              725  TGGCCA              
EaeI          458  TGGCCA               [CT]^GGCC[GA]
              725  TGGCCA              
EcoT14I       371  CCAAGG               C^C[AT][AT]GG
              461  CCAAGG              
FauI          475  CCCGC                CCCGC(4/6)
               56  GCGGG               
HinfI          74  GACTC                G^A[ACGT]TC
              271  GAGTC               
MaeIII        155  GTGAC                ^GT[ACGT]AC
              467  GTAAC               
MluNI         458  TGGCCA               TGG^CCA
              725  TGGCCA              
MscI          458  TGGCCA               TGG^CCA
              725  TGGCCA              
MwoI          185  GCATCCTACGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              684  GCAGCAGAAGC         
PleI           74  GACTC                GAGTC(4/5)
              271  GAGTC               
SfaNI         185  GCATC                GCATC(5/9)
              542  GCATC               
SmlI           13  CTCAAG               C^T[CT][GA]AG
               76  CTCAAG              
StyI          371  CCAAGG               C^C[AT][AT]GG
              461  CCAAGG              
TspRI         407  CAGTG                CA[GC]TG[ACGT][ACGT]^
              573  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (   46 enzymes) #
#########################################

AciI        AluI        BanII       BbvI        BcnI        
BmyI        BpmI        BsaJI       BsiYI       BslI        
BsmFI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       Cac8I       Cfr13I      DdeI        DpnII       
DraII       EarI        EcoNI       EcoO109I    EcoRII      
Fnu4HI      FokI        HaeIII      HapII       HpaII       
ItaI        Ksp632I     MboI        MboII       MnlI        
MspI        MvaI        NciI        NdeII       NlaIV       
Sau3AI      Sau96I      ScrFI       TaqI        TseI        
TthHB8I     
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