Restriction map for pF1KB8243
##################################
# NO CUT ENZYMES (  172 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvaI        AvrII       BalI        BamHI       
BanI        BbeI        BbrPI       BbvCI       BciVI       
BclI        BfrI        BlnI        BlpI        BpmI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiWI       
BsmBI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DraI        DraIII      DrdI        DsaI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FauI        FbaI        FseI        
HaeII       HgaI        Hin1I       HincII      HindII      
HindIII     HpaI        KasI        KpnI        Ksp632I     
KspI        MamI        MfeI        MluI        MluNI       
MroI        MscI        MspA1I      MunI        MvnI        
NarI        NcoI        NdeI        NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        SexAI       SfaNI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        Tsp45I      Tth111I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AspHI         592  GAGCAC               G[AT]GC[AT]^C
AviII           6  TGCGCA               TGC^GCA
BanII          16  GAGCCC               G[GA]GC[CT]^C
BbsI          409  GTCTTC               GAAGAC(2/6)
BcgI          441  CGACATTTCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI          627  CCCGG                CC^[GC]GG
BglI          303  GCCTAACTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         318  AGATCT               A^GATCT
BpuAI         409  GTCTTC               GAAGAC(2/6)
BsaI          509  GAGACC               GGTCTC(1/5)
BsiHKAI       592  GAGCAC               G[AT]GC[AT]^C
BsmAI         509  GAGAC                GTCTC(1/5)
BsmFI         191  GGGAC                GGGAC(10/14)
BsrFI         199  GCCGGC               [GA]^CCGG[CT]
BstYI         318  AGATCT               [GA]^GATC[CT]
CfoI            7  GCGC                 GCG^C
Cfr10I        199  GCCGGC               [GA]^CCGG[CT]
DpnII         319  GATC                 ^GATC
DraII         168  AGGACCT              [GA]G^G[ACGT]CC[CT]
EaeI          201  CGGCCA               [CT]^GGCC[GA]
EcoO109I      168  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoT14I       125  CCTTGG               C^C[AT][AT]GG
FspI            6  TGCGCA               TGC^GCA
HaeIII        202  GGCC                 GG^CC
HhaI            7  GCGC                 GCG^C
HinP1I          7  GCGC                 G^CGC
HinfI         426  GAATC                G^A[ACGT]TC
MaeII          84  ACGT                 A^CGT
MboI          319  GATC                 ^GATC
MflI          318  AGATCT               [GA]^GATC[CT]
MslI          258  CATCATAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI          199  GCCGGC               GCC^GGC
NciI          627  CCCGG                CC^[GC]GG
NdeII         319  GATC                 ^GATC
NgoMIV        199  GCCGGC               G^CCGGC
NlaIV         567  GGACCC               GG[ACGT]^[ACGT]CC
PpuMI         168  AGGACCT              [GA]G^G[AT]CC[CT]
Sau3AI        319  GATC                 ^GATC
ScaI          434  AGTACT               AGT^ACT
StyI          125  CCTTGG               C^C[AT][AT]GG
TaqI          280  TCGA                 T^CGA
TfiI          426  GAATC                G^A[AT]TC
TspRI         250  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       280  TCGA                 T^CGA
XhoII         318  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AluI          500  AGCT                 AG^CT
              573  AGCT                
BbvI          580  GCAGC                GCAGC(8/12)
                4  GCTGC               
BfaI          148  CTAG                 C^TAG
              494  CTAG                
BmyI           16  GAGCCC               G[AGT]GC[ACT]^C
              592  GAGCAC              
BsaJI          20  CCCAGG               C^C[ACGT][ACGT]GG
              125  CCTTGG              
Bsp1286I       16  GAGCCC               G[AGT]GC[ACT]^C
              592  GAGCAC              
BstNI          21  CCAGG                CC^[AT]GG
              204  CCAGG               
EcoRII         21  CCAGG                ^CC[AT]GG
              204  CCAGG               
Fnu4HI          4  GCTGC                GC^[ACGT]GC
              580  GCAGC               
HapII         200  CCGG                 C^CGG
              628  CCGG                
HpaII         200  CCGG                 C^CGG
              628  CCGG                
HphI           49  GGTGA                GGTGA(8/7)
               97  GGTGA               
ItaI            4  GCTGC                GC^[ACGT]GC
              580  GCAGC               
MaeI          148  CTAG                 C^TAG
              494  CTAG                
MseI          176  TTAA                 T^TAA
              377  TTAA                
MspI          200  CCGG                 C^CGG
              628  CCGG                
MvaI           21  CCAGG                CC^[AT]GG
              204  CCAGG               
NlaIII        534  CATG                 CATG^
              564  CATG                
Tru9I         176  TTAA                 T^TAA
              377  TTAA                
TseI            4  GCTGC                G^C[AT]GC
              580  GCAGC               


#########################################
# 3 OR MORE CUT ENZYMES (   21 enzymes) #
#########################################

AcsI        AfaI        ApoI        AvaII       BsiYI       
BslI        BsrI        BssKI       BstF5I      Cac8I       
Cfr13I      DdeI        FokI        MaeIII      MboII       
MnlI        MwoI        RsaI        Sau96I      ScrFI       
Tsp509I     
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