Restriction map for pF1KB8233
##################################
# NO CUT ENZYMES (  193 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AcyI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AvaI        AvaII       AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbsI        BcgI        BciVI       
BclI        BcnI        BfaI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiWI       
BsmBI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      Bst1107I    
BstAPI      BstBI       BstEII      BstF5I      BstPI       
BstUI       BstXI       BstZ17I     CelII       CfoI        
Cfr10I      Cfr13I      ClaI        CpoI        DraI        
DraII       DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoNI       EcoO109I    EcoO65I     EcoT14I     EcoT22I     
FauI        FbaI        FokI        FseI        FspI        
HaeII       HapII       HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        HpaII       
KasI        KpnI        KspI        MaeI        MaeIII      
MamI        MfeI        MluI        MluNI       MroI        
MscI        MseI        MslI        MspI        MunI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NlaIV       NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshBI       PvuI        
RcaI        RsaI        RsrII       SacI        SacII       
SalI        SapI        Sau96I      SbfI        ScaI        
SexAI       SfaNI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        TaqI        Tru9I       Tsp45I      
TspRI       Tth111I     TthHB8I     XbaI        XcmI        
XhoI        XmaCI       XmaI        


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# ONE CUT ENZYMES (   39 enzymes) #
###################################

AciI          399  GCGG                 CCGC(-3/-1)
AclI          362  AACGTT               AA^CGTT
AlwI          129  GGATC                GGATC(4/5)
AocI           44  CCTCAGG              CC^T[ACGT]AGG
AspHI         229  GAGCAC               G[AT]GC[AT]^C
BbvCI         196  GCTGAGG              CCTCAGC(-5/-2)
BmyI          229  GAGCAC               G[AGT]GC[ACT]^C
BsiHKAI       229  GAGCAC               G[AT]GC[AT]^C
BsiYI         306  CCACAAAATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          306  CCACAAAATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI         433  GAGAC                GTCTC(1/5)
BsmFI         186  GTCCC                GGGAC(10/14)
Bsp1286I      229  GAGCAC               G[AGT]GC[ACT]^C
BssSI         232  CACGAG               CACGAG(-5/-1)
BstYI         129  GGATCT               [GA]^GATC[CT]
Bsu36I         44  CCTCAGG              CC^T[ACGT]AGG
DpnII         130  GATC                 ^GATC
Eco81I         44  CCTCAGG              CC^T[ACGT]AGG
EcoRI         100  GAATTC               G^AATTC
EcoRV          13  GATATC               GAT^ATC
HaeIII         49  GGCC                 GG^CC
HgaI          179  GACGC                GACGC(5/10)
HphI           21  GGTGA                GGTGA(8/7)
MaeII         363  ACGT                 A^CGT
MboI          130  GATC                 ^GATC
MflI          129  GGATCT               [GA]^GATC[CT]
MspA1I        211  CAGCTG               C[AC]G^C[GT]G
MwoI          340  GCACAAATGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII         130  GATC                 ^GATC
NlaIII        190  CATG                 CATG^
PflMI         306  CCACAAAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI         179  GACGCAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I      362  AACGTT               AA^CGTT
PstI          167  CTGCAG               CTGCA^G
PvuII         211  CAGCTG               CAG^CTG
Sau3AI        130  GATC                 ^GATC
SfcI          167  CTGCAG               C^T[GA][CT]AG
Van91I        306  CCACAAAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         129  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

AcsI          100  GAATTC               [GA]^AATT[CT]
              146  AAATTC              
ApoI          100  GAATTC               [GA]^AATT[CT]
              146  AAATTC              
Asp700I        88  GAATCTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              241  GAAGTGCTTC          
BpmI           31  CTGGAG               CTGGAG(16/14)
              139  CTGGAG              
BsaJI          76  CCTCGG               C^C[ACGT][ACGT]GG
              137  CCCTGG              
BsrI           30  ACTGG                ACTGG(1/-1)
              357  ACTGG               
Cac8I          39  GCGTGC               GC[ACGT]^[ACGT]GC
              213  GCTGGC              
EarI          262  GAAGAG               CTCTTC(1/4)
              292  GAAGAG              
Ksp632I       262  GAAGAG               CTCTTC(1/4)
              292  GAAGAG              
XmnI           88  GAATCTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              241  GAAGTGCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   16 enzymes) #
#########################################

AluI        BbvI        BssKI       BstNI       DdeI        
EcoRII      Fnu4HI      HinfI       ItaI        MboII       
MnlI        MvaI        ScrFI       TfiI        TseI        
Tsp509I     
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