Restriction map for pF1KB8233
 (Circular) (Six-base) MAPSORT of: pF1KB8233.seq  Check: 9915  from: 1  to: 3560



pF1KB8233  3560 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:28  ..

AccI GT'mk_AC

Cuts at:    598     598 
   Size:       3560
 
Acc65I G'GTAC_C

Cuts at:    576     576 
   Size:       3560
 
AclI AA'CG_TT

Cuts at:    471    3400     471 
   Size:       2929     631
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    340     369     566    1385    1817    2564    2832     340 
   Size:         29     197     819     432     747     268    1068

  Fragments arranged by size: 

                1068     819     747     432     268     197      29
 
AflIII A'CryG_T

Cuts at:    912    2016    2993     912 
   Size:       1104     977    1479

  Fragments arranged by size: 

                1479    1104     977
 
AgeI A'CCGG_T

Cuts at:   1948    1948 
   Size:       3560
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    505     537     505 
   Size:         32    3528
 
AlwNI CAG_nnn'CTG

Cuts at:    865    2432     865 
   Size:       1567    1993
 
ApaLI G'TGCA_C

Cuts at:   2330    2330 
   Size:       3560
 
ApoI r'AATT_y

Cuts at:    208     254     564    2913    3169     208 
   Size:         46     310    2349     256     599

  Fragments arranged by size: 

                2349     599     310     256      46
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3560
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3560
 
AvaI C'yCGr_G

Cuts at:    580     580 
   Size:       3560
 
BamHI G'GATC_C

Cuts at:    585     585 
   Size:       3560
 
BanI G'GyrC_C

Cuts at:    576     990    1238    1273     576 
   Size:        414     248      35    2863

  Fragments arranged by size: 

                2863     414     248      35
 
BanII G_rGCy'C

Cuts at:    574    1604     574 
   Size:       1030    2530
 
BbeI G_GCGC'C

Cuts at:    994    1242     994 
   Size:        248    3312
 
BbvCI CC'TCA_GC

Cuts at:    305     305 
   Size:       3560
 
BciVI GTATCCnnnnn_n'

Cuts at:   1458    2219    3560    1458 
   Size:        761    1341    1458

  Fragments arranged by size: 

                1458    1341     761
 
BclI T'GATC_A

Cuts at:   1080    1080 
   Size:       3560
 
BglII A'GATC_T

Cuts at:   1075    3112    1075 
   Size:       2037    1523
 
BlpI GC'TnA_GC

Cuts at:    672     672 
   Size:       3560
 
Bme1580I G_kGCm'C

Cuts at:   1185    1278    2334    1185 
   Size:         93    1056    2411

  Fragments arranged by size: 

                2411    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    772     853    1188    3332     772 
   Size:         81     335    2144    1000

  Fragments arranged by size: 

                2144    1000     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1927    1927 
   Size:       3560
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    160     268     160 
   Size:        108    3452
 
Bpu10I CC'TnA_GC

Cuts at:    305     305 
   Size:       3560
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    736    1615    2107    2405     736 
   Size:        879     492     298    1891

  Fragments arranged by size: 

                1891     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3264    3264 
   Size:       3560
 
BsaAI yAC'GTr

Cuts at:    913     913 
   Size:       3560
 
BsaBI GATnn'nnATC

Cuts at:      6    1074    3111       6 
   Size:       1068    2037     455

  Fragments arranged by size: 

                2037    1068     455
 
BsaHI Gr'CG_yC

Cuts at:    991    1239    1941    3437     991 
   Size:        248     702    1496    1114

  Fragments arranged by size: 

                1496    1114     702     248
 
BsaWI w'CCGG_w

Cuts at:    749    1270    1948    2222    2369    2780     749 
   Size:        521     678     274     147     411    1529

  Fragments arranged by size: 

                1529     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:    985    1743    2320    2816     985 
   Size:        758     577     496    1729

  Fragments arranged by size: 

                1729     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1102    1148    2356     104 
   Size:        998      46    1208    1308

  Fragments arranged by size: 

                1308    1208     998      46
 
BsiHKAI G_wGCw'C

Cuts at:    341     574    1352    1542    2334     341 
   Size:        233     778     190     792    1567

  Fragments arranged by size: 

                1567     792     778     233     190
 
Bsp1286I G_dGCh'C

Cuts at:    341     574    1185    1278    1352    1542    1604    2334     341 
   Size:        233     611      93      74     190      62     730    1567

  Fragments arranged by size: 

                1567     730     611     233     190      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   2780    2780 
   Size:       3560
 
BspHI T'CATG_A

Cuts at:   2736    2736 
   Size:       3560
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    610     824    1126    1507     610 
   Size:        214     302     381    2663

  Fragments arranged by size: 

                2663     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1852    1906    3390    3555      50 
   Size:       1802      54    1484     165      55

  Fragments arranged by size: 

                1802    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1472    1472 
   Size:       3560
 
BsrFI r'CCGG_y

Cuts at:   1558    1739    1948    1558 
   Size:        181     209    3170

  Fragments arranged by size: 

                3170     209     181
 
BssSI C'ACGA_G

Cuts at:    340    1831    2189     340 
   Size:       1491     358    1711

  Fragments arranged by size: 

                1711    1491     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:    937     937 
   Size:       3560
 
BstBI TT'CG_AA

Cuts at:   1921    1921 
   Size:       3560
 
BstYI r'GATC_y

Cuts at:      1     237     585    1052    1075    1410    1656    2668    2754 
   Size:        236     348     467      23     335     246    1012      86

Cuts at:   2754    2766    3112       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     467     449     348     346     335     246     236
                  86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    153     153 
   Size:       3560
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       3560
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1510    1691    1854    1510 
   Size:        181     163    3216

  Fragments arranged by size: 

                3216     181     163
 
BtsI GCAGTG_nn'

Cuts at:    803     803 
   Size:       3560
 
DraI TTT'AAA

Cuts at:    559     559 
   Size:       3560
 
DrdI GACnn_nn'nnGTC

Cuts at:   1266    2124    1266 
   Size:        858    2702
 
EaeI y'GGCC_r

Cuts at:   1145    1319    1710    1737    3082    1145 
   Size:        174     391      27    1345    1623

  Fragments arranged by size: 

                1623    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1145    1145 
   Size:       3560
 
EarI CTCTTCn'nnn_

Cuts at:    365     395    1583    1793     365 
   Size:         30    1188     210    2132

  Fragments arranged by size: 

                2132    1188     210      30
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1768    2078    2224    3370    1768 
   Size:        310     146    1146    1958

  Fragments arranged by size: 

                1958    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    572     572 
   Size:       3560
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    160     268     340     369     566    1385    1817    2564    2832 
   Size:        108      72      29     197     819     432     747     268

Cuts at:   2832     160 
   Size:        888

  Fragments arranged by size: 

                 888     819     747     432     268     197     108      72
                  29
 
EcoO109I rG'GnC_Cy

Cuts at:    699     699 
   Size:       3560
 
EcoRI G'AATT_C

Cuts at:    208     564     208 
   Size:        356    3204
 
EcoRV GAT'ATC

Cuts at:    123     123 
   Size:       3560
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    370    1579     370 
   Size:       1209    2351
 
FspI TGC'GCA

Cuts at:   1341    1341 
   Size:       3560
 
HaeII r_GCGC'y

Cuts at:    994    1242    2264    3243     994 
   Size:        248    1022     979    1311

  Fragments arranged by size: 

                1311    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1402    1432    1434    1464    1402 
   Size:         30       2      30    3498

  Fragments arranged by size: 

                3498      30      30       2
 
HincII GTy'rAC

Cuts at:    599     599 
   Size:       3560
 
KasI G'GCGC_C

Cuts at:    990    1238     990 
   Size:        248    3312
 
KpnI G_GTAC'C

Cuts at:    580     580 
   Size:       3560
 
MluI A'CGCG_T

Cuts at:   2993    2993 
   Size:       3560
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2231    2415    2231 
   Size:        184    3376
 
MscI TGG'CCA

Cuts at:   1321    1321 
   Size:       3560
 
MslI CAynn'nnrTG

Cuts at:   1549    1676    1549 
   Size:        127    3433
 
MspA1I CmG'CkG

Cuts at:    321     671     985    1345    2358    2603     321 
   Size:        350     314     360    1013     245    1278

  Fragments arranged by size: 

                1278    1013     360     350     314     245
 
NaeI GCC'GGC

Cuts at:   1741    1741 
   Size:       3560
 
NarI GG'CG_CC

Cuts at:    991    1239     991 
   Size:        248    3312
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3560
 
NgoMIV G'CCGG_C

Cuts at:   1739    1739 
   Size:       3560
 
NspI r_CATG'y

Cuts at:    613    1113    2020     613 
   Size:        500     907    2153

  Fragments arranged by size: 

                2153     907     500
 
PciI A'CATG_T

Cuts at:   2016    2016 
   Size:       3560
 
PflMI CCAn_nnn'nTGG

Cuts at:    420     420 
   Size:       3560
 
PmeI GTTT'AAAC

Cuts at:    559     559 
   Size:       3560
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2699    2731    2699 
   Size:         32    3528
 
PshAI GACnn'nnGTC

Cuts at:    291     291 
   Size:       3560
 
PstI C_TGCA'G

Cuts at:    279     607    1292     279 
   Size:        328     685    2547

  Fragments arranged by size: 

                2547     685     328
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3560
 
PvuII CAG'CTG

Cuts at:    321     985    1345     321 
   Size:        664     360    2536

  Fragments arranged by size: 

                2536     664     360
 
RsrII CG'GwC_CG

Cuts at:   1755    1755 
   Size:       3560
 
SacI G_AGCT'C

Cuts at:    574     574 
   Size:       3560
 
SalI G'TCGA_C

Cuts at:    597     597 
   Size:       3560
 
SapI GCTCTTCn'nnn_

Cuts at:   1583    1793    1583 
   Size:        210    3350
 
SbfI CC_TGCA'GG

Cuts at:    607     607 
   Size:       3560
 
ScaI AGT'ACT

Cuts at:   3077    3077 
   Size:       3560
 
SfcI C'TryA_G

Cuts at:     33     275     603    1288    2281    2472      33 
   Size:        242     328     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     328     242     191
 
SfoI GGC'GCC

Cuts at:    992    1240     992 
   Size:        248    3312
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3560
 
SmaI CCC'GGG

Cuts at:    582     582 
   Size:       3560
 
SmlI C'TyrA_G

Cuts at:    715    1630    2122    2384     715 
   Size:        915     492     262    1891

  Fragments arranged by size: 

                1891     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3560
 
SphI G_CATG'C

Cuts at:    613    1113     613 
   Size:        500    3060
 
StyI C'CwwG_G

Cuts at:    107     694    1048     107 
   Size:        587     354    2619

  Fragments arranged by size: 

                2619     587     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    176    1143    1276    1735    1944     176 
   Size:        967     133     459     209    1792

  Fragments arranged by size: 

                1792     967     459     209     133
 
TatI w'GTAC_w

Cuts at:   3075    3075 
   Size:       3560
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3077    3109    3077 
   Size:         32    3528
 
Tth111I GACn'n_nGTC

Cuts at:   1357    1357 
   Size:       3560
 
XbaI T'CTAG_A

Cuts at:    591     591 
   Size:       3560
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1055    1055 
   Size:       3560
 
XmaI C'CCGG_G

Cuts at:    580     580 
   Size:       3560
 
XmnI GAAnn'nnTTC

Cuts at:    200     353     200 
   Size:        153    3407
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AloI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI    BbvCI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BseYI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI     FspI
    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PciI
    PflMI     PmeI     PpiI    PshAI     PstI     PvuI    PvuII    RsrII
     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI
     SmaI     SmlI     SpeI     SphI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     ApaI
     AscI    AvrII     BaeI     BbsI     BcgI    BfrBI     BglI    BmgBI
     BmtI    BsaXI    BseRI     BsgI    BsiWI     BsmI    BsmBI    BsrGI
   BssHII   BstEII    BstXI  BstZ17I     ClaI    CspCI   DraIII    EcoNI
     FseI    FspAI  HindIII     HpaI     MfeI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PasI     PfoI     PmlI    PpuMI     PsiI
   PspOMI    PspXI     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI
    SnaBI     SrfI     SspI     StuI     SwaI     XhoI     ZraI
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