Restriction map for pF1KB8220
 (Circular) (Six-base) MAPSORT of: pF1KB8220.seq  Check: 1946  from: 1  to: 4409



pF1KB8220  4409 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:28  ..

AatII G_ACGT'C

Cuts at:    452     452 
   Size:       4409
 
AccI GT'mk_AC

Cuts at:    452    1447     452 
   Size:        995    3414
 
Acc65I G'GTAC_C

Cuts at:    875    1425     875 
   Size:        550    3859
 
AclI AA'CG_TT

Cuts at:   4249    4249 
   Size:       4409
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   2234    2666    3413    3681    2234 
   Size:        432     747     268    2962

  Fragments arranged by size: 

                2962     747     432     268
 
AflIII A'CryG_T

Cuts at:   1761    2865    3842    1761 
   Size:       1104     977    2328

  Fragments arranged by size: 

                2328    1104     977
 
AgeI A'CCGG_T

Cuts at:   2797    2797 
   Size:       4409
 
AhdI GACnn_n'nnGTC

Cuts at:    763     996    1296     763 
   Size:        233     300    3876

  Fragments arranged by size: 

                3876     300     233
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    685     717     685 
   Size:         32    4377
 
AlwNI CAG_nnn'CTG

Cuts at:    549     907    1161    1714    3281     549 
   Size:        358     254     553    1567    1677

  Fragments arranged by size: 

                1677    1567     553     358     254
 
ApaLI G'TGCA_C

Cuts at:   3179    3179 
   Size:       4409
 
ApoI r'AATT_y

Cuts at:   1413    3762    4018    1413 
   Size:       2349     256    1804

  Fragments arranged by size: 

                2349    1804     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4409
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4409
 
AvaI C'yCGr_G

Cuts at:    251     302    1429     251 
   Size:         51    1127    3231

  Fragments arranged by size: 

                3231    1127      51
 
BamHI G'GATC_C

Cuts at:   1434    1434 
   Size:       4409
 
BanI G'GyrC_C

Cuts at:    208     875     965    1425    1839    2087    2122     208 
   Size:        667      90     460     414     248      35    2495

  Fragments arranged by size: 

                2495     667     460     414     248      90      35
 
BanII G_rGCy'C

Cuts at:    335     434    1423    2453     335 
   Size:         99     989    1030    2291

  Fragments arranged by size: 

                2291    1030     989      99
 
BbeI G_GCGC'C

Cuts at:    969    1843    2091     969 
   Size:        874     248    3287

  Fragments arranged by size: 

                3287     874     248
 
BbsI GAAGACnn'nnnn_

Cuts at:   1345    1345 
   Size:       4409
 
BciVI GTATCCnnnnn_n'

Cuts at:   2307    3068    4409    2307 
   Size:        761    1341    2307

  Fragments arranged by size: 

                2307    1341     761
 
BclI T'GATC_A

Cuts at:   1929    1929 
   Size:       4409
 
BfrBI ATG'CAT

Cuts at:    858     858 
   Size:       4409
 
BglI GCCn_nnn'nGGC

Cuts at:    649     649 
   Size:       4409
 
BglII A'GATC_T

Cuts at:   1924    3961    1924 
   Size:       2037    2372
 
BlpI GC'TnA_GC

Cuts at:    404     461    1521     404 
   Size:         57    1060    3292

  Fragments arranged by size: 

                3292    1060      57
 
Bme1580I G_kGCm'C

Cuts at:    279    2034    2127    3183     279 
   Size:       1755      93    1056    1505

  Fragments arranged by size: 

                1755    1505    1056      93
 
BmgBI CAC'GTC

Cuts at:    783     783 
   Size:       4409
 
BmrI ACTGGGnnnn_n'

Cuts at:   1621    1702    2037    4181    1621 
   Size:         81     335    2144    1849

  Fragments arranged by size: 

                2144    1849     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2776    2776 
   Size:       4409
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    538     556     685     706     724    1015    1045    1120     538 
   Size:         18     129      21      18     291      30      75    3827

  Fragments arranged by size: 

                3827     291     129      75      30      21      18      18
            
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    726    1105    1303    1585    2464    2956    3254     726 
   Size:        379     198     282     879     492     298    1881

  Fragments arranged by size: 

                1881     879     492     379     298     282     198
 
BsaI GGTCTCn'nnnn_

Cuts at:    292    4113     292 
   Size:       3821     588
 
BsaAI yAC'GTr

Cuts at:   1059    1762    1059 
   Size:        703    3706
 
BsaBI GATnn'nnATC

Cuts at:      6    1923    3960       6 
   Size:       1917    2037     455

  Fragments arranged by size: 

                2037    1917     455
 
BsaHI Gr'CG_yC

Cuts at:    449     844     966    1840    2088    2790    4286     449 
   Size:        395     122     874     248     702    1496     572

  Fragments arranged by size: 

                1496     874     702     572     395     248     122
 
BsaWI w'CCGG_w

Cuts at:    698    1232    1598    2119    2797    3071    3218    3629     698 
   Size:        534     366     521     678     274     147     411    1478

  Fragments arranged by size: 

                1478     678     534     521     411     366     274     147
            
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    685     715     685 
   Size:         30    4379
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    129     144     703     706     736     739    1063    1240     129 
   Size:         15     559       3      30       3     324     177    3298

  Fragments arranged by size: 

                3298     559     324     177      30      15       3       3
            
 
BseYI C'CCAG_C

Cuts at:    271    1834    2592    3169    3665     271 
   Size:       1563     758     577     496    1015

  Fragments arranged by size: 

                1563    1015     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1270    1270 
   Size:       4409
 
BsiEI CG_ry'CG

Cuts at:    104    1951    1997    3205     104 
   Size:       1847      46    1208    1308

  Fragments arranged by size: 

                1847    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    335    1391    1423    2201    2391    3183     335 
   Size:       1056      32     778     190     792    1561

  Fragments arranged by size: 

                1561    1056     792     778     190      32
 
BsmI GAATG_Cn'

Cuts at:    236     236 
   Size:       4409
 
BsmBI CGTCTCn'nnnn_

Cuts at:    111     156     111 
   Size:         45    4364
 
Bsp1286I G_dGCh'C

Cuts at:    279     335     434    1391    1423    2034    2127    2201    2391 
   Size:         56      99     957      32     611      93      74     190

Cuts at:   2391    2453    3183     279 
   Size:         62     730    1505

  Fragments arranged by size: 

                1505     957     730     611     190      99      93      74
                  62      56      32
 
BspEI T'CCGG_A

Cuts at:   3629    3629 
   Size:       4409
 
BspHI T'CATG_A

Cuts at:   3585    3585 
   Size:       4409
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    753     994    1169    1274    1459    1673    1975    2356     753 
   Size:        241     175     105     185     214     302     381    2806

  Fragments arranged by size: 

                2806     381     302     241     214     185     175     105
            
 
BsrBI CCG'CTC

Cuts at:     50     143     315     881    1053    2701    2755    4239    4404 
   Size:         93     172     566     172    1648      54    1484     165

Cuts at:   4404      50 
   Size:         55

  Fragments arranged by size: 

                1648    1484     566     172     172     165      93      55
                  54
 
BsrDI GCAATG_nn'

Cuts at:    845    2321     845 
   Size:       1476    2933
 
BsrFI r'CCGG_y

Cuts at:    962    2407    2588    2797     962 
   Size:       1445     181     209    2574

  Fragments arranged by size: 

                2574    1445     209     181
 
BssSI C'ACGA_G

Cuts at:    718    2680    3038     718 
   Size:       1962     358    2089

  Fragments arranged by size: 

                2089    1962     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:    283    1786     283 
   Size:       1503    2906
 
BstBI TT'CG_AA

Cuts at:   2770    2770 
   Size:       4409
 
BstXI CCAn_nnnn'nTGG

Cuts at:    441     441 
   Size:       4409
 
BstYI r'GATC_y

Cuts at:      1     660     695     713     818    1434    1901    1924    2259 
   Size:        659      35      18     105     616     467      23     335

Cuts at:   2259    2505    3517    3603    3615    3961       1 
   Size:        246    1012      86      12     346     449

  Fragments arranged by size: 

                1012     659     616     467     449     346     335     246
                 105      86      35      23      18      12
 
Bsu36I CC'TnA_GG

Cuts at:    156     589     156 
   Size:        433    3976
 
BtgI C'CryG_G

Cuts at:    107    1343     107 
   Size:       1236    3173
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2359    2540    2703    2359 
   Size:        181     163    4065

  Fragments arranged by size: 

                4065     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1652    1652 
   Size:       4409
 
DraI TTT'AAA

Cuts at:   1408    1408 
   Size:       4409
 
DrdI GACnn_nn'nnGTC

Cuts at:   2115    2973    2115 
   Size:        858    3551
 
EaeI y'GGCC_r

Cuts at:    189     551     785    1070    1994    2168    2559    2586    3931 
   Size:        362     234     285     924     174     391      27    1345

Cuts at:   3931     189 
   Size:        667

  Fragments arranged by size: 

                1345     924     667     391     362     285     234     174
                  27
 
EagI C'GGCC_G

Cuts at:   1994    1994 
   Size:       4409
 
EarI CTCTTCn'nnn_

Cuts at:    863    1100    2432    2642     863 
   Size:        237    1332     210    2630

  Fragments arranged by size: 

                2630    1332     237     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    922    2617    2927    3073    4219     922 
   Size:       1695     310     146    1146    1112

  Fragments arranged by size: 

                1695    1146    1112     310     146
 
EcoICRI GAG'CTC

Cuts at:    333    1421     333 
   Size:       1088    3321
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    538     556     685     706     724    1015    1045    1120    2234 
   Size:         18     129      21      18     291      30      75    1114

Cuts at:   2234    2666    3413    3681     538 
   Size:        432     747     268    1266

  Fragments arranged by size: 

                1266    1114     747     432     291     268     129      75
                  30      21      18      18
 
EcoO109I rG'GnC_Cy

Cuts at:    177     202     546    1158    1548     177 
   Size:         25     344     612     390    3038

  Fragments arranged by size: 

                3038     612     390     344      25
 
EcoRI G'AATT_C

Cuts at:   1413    1413 
   Size:       4409
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    387    2428     387 
   Size:       2041    2368
 
FspI TGC'GCA

Cuts at:   2190    2190 
   Size:       4409
 
HaeII r_GCGC'y

Cuts at:    397     512     969    1090    1843    2091    3113    4092     397 
   Size:        115     457     121     753     248    1022     979     714

  Fragments arranged by size: 

                1022     979     753     714     457     248     121     115
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    519     551     749     781     982    1014    1282    1314    2251 
   Size:         32     198      32     201      32     268      32     937

Cuts at:   2251    2281    2283    2313     519 
   Size:         30       2      30    2615

  Fragments arranged by size: 

                2615     937     268     201     198      32      32      32
                  32      30      30       2
 
HincII GTy'rAC

Cuts at:   1448    1448 
   Size:       4409
 
KasI G'GCGC_C

Cuts at:    965    1839    2087     965 
   Size:        874     248    3287

  Fragments arranged by size: 

                3287     874     248
 
KpnI G_GTAC'C

Cuts at:    879    1429     879 
   Size:        550    3859
 
MluI A'CGCG_T

Cuts at:   3842    3842 
   Size:       4409
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1286    3080    3264    1286 
   Size:       1794     184    2431

  Fragments arranged by size: 

                2431    1794     184
 
MscI TGG'CCA

Cuts at:    553     787    2170     553 
   Size:        234    1383    2792

  Fragments arranged by size: 

                2792    1383     234
 
MslI CAynn'nnrTG

Cuts at:   1246    1333    1394    2398    2525    1246 
   Size:         87      61    1004     127    3130

  Fragments arranged by size: 

                3130    1004     127      87      61
 
MspA1I CmG'CkG

Cuts at:    133     417     750    1520    1834    2194    3207    3452     133 
   Size:        284     333     770     314     360    1013     245    1090

  Fragments arranged by size: 

                1090    1013     770     360     333     314     284     245
            
 
NaeI GCC'GGC

Cuts at:   2590    2590 
   Size:       4409
 
NarI GG'CG_CC

Cuts at:    966    1840    2088     966 
   Size:        874     248    3287

  Fragments arranged by size: 

                3287     874     248
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       4409
 
NgoMIV G'CCGG_C

Cuts at:   2588    2588 
   Size:       4409
 
NsiI A_TGCA'T

Cuts at:    860     860 
   Size:       4409
 
NspI r_CATG'y

Cuts at:    858    1462    1962    2869     858 
   Size:        604     500     907    2398

  Fragments arranged by size: 

                2398     907     604     500
 
PciI A'CATG_T

Cuts at:   2865    2865 
   Size:       4409
 
PfoI T'CCnGG_A

Cuts at:    374     572     740     374 
   Size:        198     168    4043

  Fragments arranged by size: 

                4043     198     168
 
PmeI GTTT'AAAC

Cuts at:   1408    1408 
   Size:       4409
 
PmlI CAC'GTG

Cuts at:   1059    1059 
   Size:       4409
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    685     717    3548    3580     685 
   Size:         32    2831      32    1514

  Fragments arranged by size: 

                2831    1514      32      32
 
PpuMI rG'GwC_Cy

Cuts at:    202     546    1158     202 
   Size:        344     612    3453

  Fragments arranged by size: 

                3453     612     344
 
PstI C_TGCA'G

Cuts at:   1271    1456    2141    1271 
   Size:        185     685    3539

  Fragments arranged by size: 

                3539     685     185
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4409
 
PvuII CAG'CTG

Cuts at:    417     750    1834    2194     417 
   Size:        333    1084     360    2632

  Fragments arranged by size: 

                2632    1084     360     333
 
RsrII CG'GwC_CG

Cuts at:   2604    2604 
   Size:       4409
 
SacI G_AGCT'C

Cuts at:    335    1423     335 
   Size:       1088    3321
 
SalI G'TCGA_C

Cuts at:   1446    1446 
   Size:       4409
 
SapI GCTCTTCn'nnn_

Cuts at:   2432    2642    2432 
   Size:        210    4199
 
SbfI CC_TGCA'GG

Cuts at:   1456    1456 
   Size:       4409
 
ScaI AGT'ACT

Cuts at:   3926    3926 
   Size:       4409
 
SfcI C'TryA_G

Cuts at:     33     621    1203    1267    1452    2137    3130    3321      33 
   Size:        588     582      64     185     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     588     582     191     185      64
            
 
SfoI GGC'GCC

Cuts at:    967    1841    2089     967 
   Size:        874     248    3287

  Fragments arranged by size: 

                3287     874     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4409
 
SmaI CCC'GGG

Cuts at:    253     304    1431     253 
   Size:         51    1127    3231

  Fragments arranged by size: 

                3231    1127      51
 
SmlI C'TyrA_G

Cuts at:    705    1120    1318    1564    2479    2971    3233     705 
   Size:        415     198     246     915     492     262    1881

  Fragments arranged by size: 

                1881     915     492     415     262     246     198
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4409
 
SphI G_CATG'C

Cuts at:   1462    1962    1462 
   Size:        500    3909
 
StyI C'CwwG_G

Cuts at:    107     425    1543    1897     107 
   Size:        318    1118     354    2619

  Fragments arranged by size: 

                2619    1118     354     318
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1992    2125    2584    2793    1992 
   Size:        133     459     209    3608

  Fragments arranged by size: 

                3608     459     209     133
 
TatI w'GTAC_w

Cuts at:   3924    3924 
   Size:       4409
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3926    3958    3926 
   Size:         32    4377
 
Tth111I GACn'n_nGTC

Cuts at:   2206    2206 
   Size:       4409
 
XbaI T'CTAG_A

Cuts at:   1440    1440 
   Size:       4409
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    591    1904     591 
   Size:       1313    3096
 
XmaI C'CCGG_G

Cuts at:    251     302    1429     251 
   Size:         51    1127    3231

  Fragments arranged by size: 

                3231    1127      51
 
ZraI GAC'GTC

Cuts at:    450     450 
   Size:       4409
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AhdI
     AloI    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI
     BanI    BanII     BbeI     BbsI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstXI
    BstYI   Bsu36I     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NsiI
     NspI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI     PstI
     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI
     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI    AflII     AleI     AlfI     ApaI     AscI    AvrII
     BaeI    BbvCI     BcgI     BmtI   Bpu10I    BsiWI    BsrGI   BssHII
   BstEII  BstZ17I     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI
    FspAI  HindIII     HpaI     MfeI     NdeI     NheI     NotI     NruI
     PacI     PasI    PflMI    PshAI     PsiI   PspOMI    PspXI     PsrI
    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI
     StuI     SwaI     XhoI     XmnI
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