Restriction map for pF1KB8220
 (Linear) (Six-base) MAPSORT of: KIBB8220.seq  Check: 3422  from: 1  to: 1296



KIBB8220.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:04  ..

AatII G_ACGT'C

Cuts at:      0     344    1296 
   Size:        344     952
 
AccI GT'mk_AC

Cuts at:      0     344    1296 
   Size:        344     952
 
Acc65I G'GTAC_C

Cuts at:      0     767    1296 
   Size:        767     529
 
AhdI GACnn_n'nnGTC

Cuts at:      0     655     888    1188    1296 
   Size:        655     233     300     108

  Fragments arranged by size: 

                 655     300     233     108
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     577     609    1296 
   Size:        577      32     687

  Fragments arranged by size: 

                 687     577      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     441     799    1053    1296 
   Size:        441     358     254     243

  Fragments arranged by size: 

                 441     358     254     243
 
AvaI C'yCGr_G

Cuts at:      0     143     194    1296 
   Size:        143      51    1102

  Fragments arranged by size: 

                1102     143      51
 
BanI G'GyrC_C

Cuts at:      0     100     767     857    1296 
   Size:        100     667      90     439

  Fragments arranged by size: 

                 667     439     100      90
 
BanII G_rGCy'C

Cuts at:      0     227     326    1296 
   Size:        227      99     970

  Fragments arranged by size: 

                 970     227      99
 
BbeI G_GCGC'C

Cuts at:      0     861    1296 
   Size:        861     435
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1237    1296 
   Size:       1237      59
 
BfrBI ATG'CAT

Cuts at:      0     750    1296 
   Size:        750     546
 
BglI GCCn_nnn'nGGC

Cuts at:      0     541    1296 
   Size:        541     755
 
BlpI GC'TnA_GC

Cuts at:      0     296     353    1296 
   Size:        296      57     943

  Fragments arranged by size: 

                 943     296      57
 
Bme1580I G_kGCm'C

Cuts at:      0     171    1296 
   Size:        171    1125
 
BmgBI CAC'GTC

Cuts at:      0     675    1296 
   Size:        675     621
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     430     448     577     598     616     907     937    1012 
   Size:        430      18     129      21      18     291      30      75

Cuts at:   1012    1296 
   Size:        284

  Fragments arranged by size: 

                 430     291     284     129      75      30      21      18
                  18
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     618     997    1195    1296 
   Size:        618     379     198     101

  Fragments arranged by size: 

                 618     379     198     101
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     184    1296 
   Size:        184    1112
 
BsaAI yAC'GTr

Cuts at:      0     951    1296 
   Size:        951     345
 
BsaHI Gr'CG_yC

Cuts at:      0     341     736     858    1296 
   Size:        341     395     122     438

  Fragments arranged by size: 

                 438     395     341     122
 
BsaWI w'CCGG_w

Cuts at:      0     590    1124    1296 
   Size:        590     534     172

  Fragments arranged by size: 

                 590     534     172
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     577     607    1296 
   Size:        577      30     689

  Fragments arranged by size: 

                 689     577      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      21      36     595     598     628     631     955    1132 
   Size:         21      15     559       3      30       3     324     177

Cuts at:   1132    1296 
   Size:        164

  Fragments arranged by size: 

                 559     324     177     164      30      21      15       3
                   3
 
BseYI C'CCAG_C

Cuts at:      0     163    1296 
   Size:        163    1133
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1162    1296 
   Size:       1162     134
 
BsiHKAI G_wGCw'C

Cuts at:      0     227    1283    1296 
   Size:        227    1056      13

  Fragments arranged by size: 

                1056     227      13
 
BsmI GAATG_Cn'

Cuts at:      0     128    1296 
   Size:        128    1168
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0       3      48    1296 
   Size:          3      45    1248

  Fragments arranged by size: 

                1248      45       3
 
Bsp1286I G_dGCh'C

Cuts at:      0     171     227     326    1283    1296 
   Size:        171      56      99     957      13

  Fragments arranged by size: 

                 957     171      99      56      13
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     645     886    1061    1166    1296 
   Size:        645     241     175     105     130

  Fragments arranged by size: 

                 645     241     175     130     105
 
BsrBI CCG'CTC

Cuts at:      0      35     207     773     945    1296 
   Size:         35     172     566     172     351

  Fragments arranged by size: 

                 566     351     172     172      35
 
BsrDI GCAATG_nn'

Cuts at:      0     737    1296 
   Size:        737     559
 
BsrFI r'CCGG_y

Cuts at:      0     854    1296 
   Size:        854     442
 
BssSI C'ACGA_G

Cuts at:      0     610    1296 
   Size:        610     686
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     175    1296 
   Size:        175    1121
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     333    1296 
   Size:        333     963
 
BstYI r'GATC_y

Cuts at:      0     552     587     605     710    1296 
   Size:        552      35      18     105     586

  Fragments arranged by size: 

                 586     552     105      35      18
 
Bsu36I CC'TnA_GG

Cuts at:      0      48     481    1296 
   Size:         48     433     815

  Fragments arranged by size: 

                 815     433      48
 
BtgI C'CryG_G

Cuts at:      0    1235    1296 
   Size:       1235      61
 
EaeI y'GGCC_r

Cuts at:      0      81     443     677     962    1296 
   Size:         81     362     234     285     334

  Fragments arranged by size: 

                 362     334     285     234      81
 
EarI CTCTTCn'nnn_

Cuts at:      0     755     992    1296 
   Size:        755     237     304

  Fragments arranged by size: 

                 755     304     237
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     814    1296 
   Size:        814     482
 
EcoICRI GAG'CTC

Cuts at:      0     225    1296 
   Size:        225    1071
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     430     448     577     598     616     907     937    1012 
   Size:        430      18     129      21      18     291      30      75

Cuts at:   1012    1296 
   Size:        284

  Fragments arranged by size: 

                 430     291     284     129      75      30      21      18
                  18
 
EcoO109I rG'GnC_Cy

Cuts at:      0      69      94     438    1050    1296 
   Size:         69      25     344     612     246

  Fragments arranged by size: 

                 612     344     246      69      25
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     279    1296 
   Size:        279    1017
 
HaeII r_GCGC'y

Cuts at:      0     289     404     861     982    1296 
   Size:        289     115     457     121     314

  Fragments arranged by size: 

                 457     314     289     121     115
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     411     443     641     673     874     906    1174    1206 
   Size:        411      32     198      32     201      32     268      32

Cuts at:   1206    1296 
   Size:         90

  Fragments arranged by size: 

                 411     268     201     198      90      32      32      32
                  32
 
KasI G'GCGC_C

Cuts at:      0     857    1296 
   Size:        857     439
 
KpnI G_GTAC'C

Cuts at:      0     771    1296 
   Size:        771     525
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1178    1296 
   Size:       1178     118
 
MscI TGG'CCA

Cuts at:      0     445     679    1296 
   Size:        445     234     617

  Fragments arranged by size: 

                 617     445     234
 
MslI CAynn'nnrTG

Cuts at:      0    1138    1225    1286    1296 
   Size:       1138      87      61      10

  Fragments arranged by size: 

                1138      87      61      10
 
MspA1I CmG'CkG

Cuts at:      0      25     309     642    1296 
   Size:         25     284     333     654

  Fragments arranged by size: 

                 654     333     284      25
 
NarI GG'CG_CC

Cuts at:      0     858    1296 
   Size:        858     438
 
NsiI A_TGCA'T

Cuts at:      0     752    1296 
   Size:        752     544
 
NspI r_CATG'y

Cuts at:      0     750    1296 
   Size:        750     546
 
PfoI T'CCnGG_A

Cuts at:      0     266     464     632    1296 
   Size:        266     198     168     664

  Fragments arranged by size: 

                 664     266     198     168
 
PmlI CAC'GTG

Cuts at:      0     951    1296 
   Size:        951     345
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     577     609    1296 
   Size:        577      32     687

  Fragments arranged by size: 

                 687     577      32
 
PpuMI rG'GwC_Cy

Cuts at:      0      94     438    1050    1296 
   Size:         94     344     612     246

  Fragments arranged by size: 

                 612     344     246      94
 
PstI C_TGCA'G

Cuts at:      0    1163    1296 
   Size:       1163     133
 
PvuII CAG'CTG

Cuts at:      0     309     642    1296 
   Size:        309     333     654

  Fragments arranged by size: 

                 654     333     309
 
SacI G_AGCT'C

Cuts at:      0     227    1296 
   Size:        227    1069
 
SfcI C'TryA_G

Cuts at:      0     513    1095    1159    1296 
   Size:        513     582      64     137

  Fragments arranged by size: 

                 582     513     137      64
 
SfoI GGC'GCC

Cuts at:      0     859    1296 
   Size:        859     437
 
SmaI CCC'GGG

Cuts at:      0     145     196    1296 
   Size:        145      51    1100

  Fragments arranged by size: 

                1100     145      51
 
SmlI C'TyrA_G

Cuts at:      0     597    1012    1210    1296 
   Size:        597     415     198      86

  Fragments arranged by size: 

                 597     415     198      86
 
StyI C'CwwG_G

Cuts at:      0     317    1296 
   Size:        317     979
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     483    1296 
   Size:        483     813
 
XmaI C'CCGG_G

Cuts at:      0     143     194    1296 
   Size:        143      51    1102

  Fragments arranged by size: 

                1102     143      51
 
ZraI GAC'GTC

Cuts at:      0     342    1296 
   Size:        342     954
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AhdI     AloI    AlwNI     AvaI     BanI
    BanII     BbeI     BbsI    BfrBI     BglI     BlpI Bme1580I    BmgBI
     BpmI    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspMI    BsrBI
    BsrDI    BsrFI    BssSI   BstAPI    BstXI    BstYI   Bsu36I     BtgI
     EaeI     EarI     EciI  EcoICRI  Eco57MI EcoO109I     FalI    HaeII
    Hin4I     KasI     KpnI     MmeI     MscI     MslI   MspA1I     NarI
     NsiI     NspI     PfoI     PmlI     PpiI    PpuMI     PstI    PvuII
     SacI     SfcI     SfoI     SmaI     SmlI     StyI     XcmI     XmaI
     ZraI

 Enzymes that do not cut: 

     AarI     AclI     AcuI     AfeI    AflII   AflIII     AgeI     AleI
     AlfI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI    BbvCI     BcgI    BciVI     BclI    BglII     BmrI
     BmtI     BplI   Bpu10I    BsaBI    BsiEI    BsiWI    BspEI    BspHI
    BsrGI   BssHII    BstBI   BstEII  BstZ17I    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI    EcoNI    EcoRI    EcoRV
     FseI     FspI    FspAI   HincII  HindIII     HpaI     MfeI     MluI
     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     PacI
     PasI     PciI    PflMI     PmeI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SgfI    SgrAI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XhoI     XmnI
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