Restriction map for pF1KB8219
##################################
# NO CUT ENZYMES (  140 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaLI       ApoI        AscI        
AseI        AsnI        Asp718I     AspEI       AspHI       
AspI        AvrII       BamHI       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BfaI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaHI       
BsaWI       BseAI       BsiEI       BsiHKAI     BsiWI       
Bsp1407I    BspDI       BspEI       BspHI       BsrBI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DraI        DraIII      DsaI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       EcoT22I     FseI        HaeII       
HgaI        Hin1I       HincII      HindII      HindIII     
HpaI        KasI        KpnI        KspI        MaeI        
MaeII       MfeI        MluI        MroI        MunI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        SwaI        TfiI        Tsp509I     Tth111I     
XbaI        XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   57 enzymes) #
###################################

AccII         142  CGCG                 CG^CG
AflII          43  CTTAAG               C^TTAAG
AflIII        299  ACATGT               A^C[GA][CT]GT
ApaI          391  GGGCCC               GGGCC^C
Asp700I        75  GAAATGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII         279  GGACC                G^G[AT]CC
AviII         182  TGCGCA               TGC^GCA
BalI          308  TGGCCA               TGG^CCA
BclI          479  TGATCA               T^GATCA
BcnI          246  CCGGG                CC^[GC]GG
BfrI           43  CTTAAG               C^TTAAG
BsaBI          64  GATGATTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI         913  GAGGAG               GAGGAG(10/8)
BsgI          472  GTGCAG               GTGCAG(16/14)
BsmBI         760  GAGACG               CGTCTC(1/5)
BsmI          633  GCATTC               GAATGC(1/-1)
BspLU11I      299  ACATGT               A^CATGT
BspMI         570  GCAGGT               ACCTGC(4/8)
BstUI         142  CGCG                 CG^CG
BstXI         375  CCAGAACACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         125  AGATCC               [GA]^GATC[CT]
CfoI          183  GCGC                 GCG^C
DraII         278  AGGACCT              [GA]G^G[ACGT]CC[CT]
DrdI           21  GACCTGGAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI          308  TGGCCA               [CT]^GGCC[GA]
EarI          904  GAAGAG               CTCTTC(1/4)
EcoO109I      278  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoRV         613  GATATC               GAT^ATC
FauI          143  GCGGG                CCCGC(4/6)
FbaI          479  TGATCA               T^GATCA
FspI          182  TGCGCA               TGC^GCA
HapII         246  CCGG                 C^CGG
HhaI          183  GCGC                 GCG^C
HinP1I        183  GCGC                 G^CGC
HpaII         246  CCGG                 C^CGG
Ksp632I       904  GAAGAG               CTCTTC(1/4)
MaeIII        361  GTCAC                ^GT[ACGT]AC
MamI           64  GATGATTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          125  AGATCC               [GA]^GATC[CT]
MluNI         308  TGGCCA               TGG^CCA
MscI          308  TGGCCA               TGG^CCA
MseI           44  TTAA                 T^TAA
MslI          174  CACCATGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          246  CCGG                 C^CGG
MvnI          142  CGCG                 CG^CG
NciI          246  CCGGG                CC^[GC]GG
PflMI          54  CCAACTATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         278  AGGACCT              [GA]G^G[AT]CC[CT]
SmlI           43  CTTAAG               C^T[CT][GA]AG
StuI          797  AGGCCT               AGG^CCT
TaqI          578  TCGA                 T^CGA
Tru9I          44  TTAA                 T^TAA
Tsp45I        361  GTCAC                ^GT[GC]AC
TthHB8I       578  TCGA                 T^CGA
Van91I         54  CCAACTATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         125  AGATCC               [GA]^GATC[CT]
XmnI           75  GAAATGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AfaI          709  GTAC                 GT^AC
              732  GTAC                
AlwI          126  GATCC                GGATC(4/5)
              811  GATCC               
AlwNI         323  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              669  CAGTGTCTG           
AvaI          388  CTCGGG               C^[CT]CG[GA]G
              394  CCCGAG              
BanII         105  GGGCTC               G[GA]GC[CT]^C
              391  GGGCCC              
BbsI           10  GAAGAC               GAAGAC(2/6)
              399  GAAGAC              
BpuAI          10  GAAGAC               GAAGAC(2/6)
              399  GAAGAC              
BsaI          385  GGTCTC               GGTCTC(1/5)
              940  GGTCTC              
BsmFI         296  GGGAC                GGGAC(10/14)
              135  GTCCC               
BsoBI         388  CTCGGG               C^[CT]CG[GA]G
              394  CCCGAG              
BsrDI         345  CATTGC               GCAATG(2/0)
              659  GCAATG              
EcoT14I       272  CCAAGG               C^C[AT][AT]GG
              899  CCAAGG              
HphI          269  TCACC                GGTGA(8/7)
              428  TCACC               
PstI          679  CTGCAG               CTGCA^G
              875  CTGCAG              
RsaI          709  GTAC                 GT^AC
              732  GTAC                
SfcI          679  CTGCAG               C^T[GA][CT]AG
              875  CTGCAG              
StyI          272  CCAAGG               C^C[AT][AT]GG
              899  CCAAGG              


#########################################
# 3 OR MORE CUT ENZYMES (   44 enzymes) #
#########################################

AciI        AluI        BanI        BbvI        BglI        
BmyI        BpmI        BsaJI       BsiYI       BslI        
BsmAI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       Cac8I       Cfr13I      DdeI        DpnII       
EcoRII      Fnu4HI      FokI        HaeIII      HinfI       
ItaI        MboI        MboII       MnlI        MspA1I      
MvaI        MwoI        NdeII       NlaIII      NlaIV       
NspI        PleI        PvuII       Sau3AI      Sau96I      
ScrFI       SfaNI       TseI        TspRI       
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