Restriction map for pF1KB8200
 (Linear) (Six-base) MAPSORT of: KIBB8200.seq  Check: 3761  from: 1  to: 1116



KIBB8200.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:03  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     150    1116 
   Size:        150     966
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     262    1116 
   Size:        262     854
 
AfeI AGC'GCT

Cuts at:      0     901    1116 
   Size:        901     215
 
AflIII A'CryG_T

Cuts at:      0     230    1116 
   Size:        230     886
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     568     600    1116 
   Size:        568      32     516

  Fragments arranged by size: 

                 568     516      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     602     912    1116 
   Size:        602     310     204

  Fragments arranged by size: 

                 602     310     204
 
ApaI G_GGCC'C

Cuts at:      0     183     315    1116 
   Size:        183     132     801

  Fragments arranged by size: 

                 801     183     132
 
ApaLI G'TGCA_C

Cuts at:      0     133    1116 
   Size:        133     983
 
AvaI C'yCGr_G

Cuts at:      0     184    1116 
   Size:        184     932
 
BanI G'GyrC_C

Cuts at:      0     115     449     540    1116 
   Size:        115     334      91     576

  Fragments arranged by size: 

                 576     334     115      91
 
BanII G_rGCy'C

Cuts at:      0      29     143     183     315     559     874     931    1116 
   Size:         29     114      40     132     244     315      57     185

  Fragments arranged by size: 

                 315     244     185     132     114      57      40      29
            
 
BbvCI CC'TCA_GC

Cuts at:      0     436    1116 
   Size:        436     680
 
BglII A'GATC_T

Cuts at:      0     992    1116 
   Size:        992     124
 
BlpI GC'TnA_GC

Cuts at:      0     835    1003    1116 
   Size:        835     168     113

  Fragments arranged by size: 

                 835     168     113
 
Bme1580I G_kGCm'C

Cuts at:      0     137     183     315    1116 
   Size:        137      46     132     801

  Fragments arranged by size: 

                 801     137     132      46
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     186    1116 
   Size:        186     930
 
Bpu10I CC'TnA_GC

Cuts at:      0     436     863    1116 
   Size:        436     427     253

  Fragments arranged by size: 

                 436     427     253
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     127    1090    1116 
   Size:        127     963      26

  Fragments arranged by size: 

                 963     127      26
 
BsaAI yAC'GTr

Cuts at:      0     231    1116 
   Size:        231     885
 
BsaBI GATnn'nnATC

Cuts at:      0      70     361    1116 
   Size:         70     291     755

  Fragments arranged by size: 

                 755     291      70
 
BsaHI Gr'CG_yC

Cuts at:      0     620     818    1116 
   Size:        620     198     298

  Fragments arranged by size: 

                 620     298     198
 
BseYI C'CCAG_C

Cuts at:      0     308     608    1116 
   Size:        308     300     508

  Fragments arranged by size: 

                 508     308     300
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     955    1020    1116 
   Size:        955      65      96

  Fragments arranged by size: 

                 955      96      65
 
BsiEI CG_ry'CG

Cuts at:      0     538    1116 
   Size:        538     578
 
BsiHKAI G_wGCw'C

Cuts at:      0     137     143     152     874     931    1004    1027    1116 
   Size:        137       6       9     722      57      73      23      89

  Fragments arranged by size: 

                 722     137      89      73      57      23       9       6
            
 
Bsp1286I G_dGCh'C

Cuts at:      0      29     137     143     152     183     315     559     874 
   Size:         29     108       6       9      31     132     244     315

Cuts at:    874     931    1004    1027    1116 
   Size:         57      73      23      89

  Fragments arranged by size: 

                 315     244     132     108      89      73      57      31
                  29      23       9       6
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     150     214    1116 
   Size:        150      64     902

  Fragments arranged by size: 

                 902     150      64
 
BsrBI CCG'CTC

Cuts at:      0     113     425    1116 
   Size:        113     312     691

  Fragments arranged by size: 

                 691     312     113
 
BsrFI r'CCGG_y

Cuts at:      0     475     537    1116 
   Size:        475      62     579

  Fragments arranged by size: 

                 579     475      62
 
BssSI C'ACGA_G

Cuts at:      0     136     350    1116 
   Size:        136     214     766

  Fragments arranged by size: 

                 766     214     136
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     602     677    1116 
   Size:        602      75     439

  Fragments arranged by size: 

                 602     439      75
 
BstBI TT'CG_AA

Cuts at:      0     587    1116 
   Size:        587     529
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1047    1116 
   Size:       1047      69
 
BstYI r'GATC_y

Cuts at:      0     500     992    1116 
   Size:        500     492     124

  Fragments arranged by size: 

                 500     492     124
 
BtgI C'CryG_G

Cuts at:      0     550     824    1116 
   Size:        550     274     292

  Fragments arranged by size: 

                 550     292     274
 
BtsI GCAGTG_nn'

Cuts at:      0     736    1116 
   Size:        736     380
 
DraIII CAC_nnn'GTG

Cuts at:      0     551    1116 
   Size:        551     565
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     673    1116 
   Size:        673     443
 
EaeI y'GGCC_r

Cuts at:      0     535     704    1116 
   Size:        535     169     412

  Fragments arranged by size: 

                 535     412     169
 
EagI C'GGCC_G

Cuts at:      0     535    1116 
   Size:        535     581
 
EarI CTCTTCn'nnn_

Cuts at:      0    1072    1116 
   Size:       1072      44
 
EcoICRI GAG'CTC

Cuts at:      0     141     872     929    1116 
   Size:        141     731      57     187

  Fragments arranged by size: 

                 731     187     141      57
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     262    1116 
   Size:        262     854
 
EcoO109I rG'GnC_Cy

Cuts at:      0     180     312    1101    1116 
   Size:        180     132     789      15

  Fragments arranged by size: 

                 789     180     132      15
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     585    1116 
   Size:        585     531
 
HaeII r_GCGC'y

Cuts at:      0     748     903    1116 
   Size:        748     155     213

  Fragments arranged by size: 

                 748     213     155
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     478    1116 
   Size:        478     638
 
MslI CAynn'nnrTG

Cuts at:      0     101    1045    1116 
   Size:        101     944      71

  Fragments arranged by size: 

                 944     101      71
 
MspA1I CmG'CkG

Cuts at:      0      35     299     344     638     781     826    1007    1116 
   Size:         35     264      45     294     143      45     181     109

  Fragments arranged by size: 

                 294     264     181     143     109      45      45      35
            
 
NaeI GCC'GGC

Cuts at:      0     477     539    1116 
   Size:        477      62     577

  Fragments arranged by size: 

                 577     477      62
 
NgoMIV G'CCGG_C

Cuts at:      0     475     537    1116 
   Size:        475      62     579

  Fragments arranged by size: 

                 579     475      62
 
NruI TCG'CGA

Cuts at:      0     712    1116 
   Size:        712     404
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     551     912    1116 
   Size:        551     361     204

  Fragments arranged by size: 

                 551     361     204
 
PpuMI rG'GwC_Cy

Cuts at:      0    1101    1116 
   Size:       1101      15
 
PspOMI G'GGCC_C

Cuts at:      0     179     311    1116 
   Size:        179     132     805

  Fragments arranged by size: 

                 805     179     132
 
PvuII CAG'CTG

Cuts at:      0      35    1116 
   Size:         35    1081
 
SacI G_AGCT'C

Cuts at:      0     143     874     931    1116 
   Size:        143     731      57     185

  Fragments arranged by size: 

                 731     185     143      57
 
SacII CC_GC'GG

Cuts at:      0     827    1116 
   Size:        827     289
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1072    1116 
   Size:       1072      44
 
SmlI C'TyrA_G

Cuts at:      0     142    1105    1116 
   Size:        142     963      11

  Fragments arranged by size: 

                 963     142      11
 
StyI C'CwwG_G

Cuts at:      0     128    1116 
   Size:        128     988
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      76     558    1116 
   Size:         76     482     558

  Fragments arranged by size: 

                 558     482      76
 

 Enzymes that do cut:

     AarI     AcuI     AfeI   AflIII     AloI    AlwNI     ApaI    ApaLI
     AvaI     BanI    BanII    BbvCI    BglII     BlpI Bme1580I     BmrI
   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BseYI     BsgI    BsiEI
  BsiHKAI Bsp1286I    BspMI    BsrBI    BsrFI    BssSI   BstAPI    BstBI
    BstXI    BstYI     BtgI     BtsI   DraIII     DrdI     EaeI     EagI
     EarI  EcoICRI  Eco57MI EcoO109I     FalI    HaeII     MmeI     MslI
   MspA1I     NaeI   NgoMIV     NruI    PflMI    PpuMI   PspOMI    PvuII
     SacI    SacII     SapI     SmlI     StyI    TaqII

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII     AgeI     AhdI     AleI
     AlfI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI     BglI    BmgBI
     BmtI     BplI     BpmI     BsaI    BsaWI    BsaXI    BseRI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrDI    BsrGI   BssHII   BstEII
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI     EciI    EcoNI
    EcoRI    EcoRV     FseI     FspI    FspAI    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     NarI     NcoI
     NdeI     NheI     NotI     NsiI     NspI     PacI     PasI     PciI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI
     PstI     PvuI    RsrII     SalI    SanDI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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