Restriction map for pF1KB8180
##################################
# NO CUT ENZYMES (  126 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflIII      AgeI        AhdI        AocI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       AvrII       BamHI       BbeI        
BbrPI       BcgI        BciVI       BclI        BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BsgI        BsiEI       
BsiWI       BsmAI       BsmBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrDI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco52I      Eco81I      EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        Hin1I       HindIII     HpaI        KasI        
KpnI        KspI        MamI        MfeI        MluI        
MroI        MunI        NarI        NdeI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TaqI        
TfiI        Tth111I     TthHB8I     XbaI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccI         1006  GTCTAC               GT^[AC][GT]AC
AcsI         1090  AAATTC               [GA]^AATT[CT]
AflII         958  CTTAAG               C^TTAAG
Alw44I        872  GTGCAC               G^TGCAC
AlwI          738  GATCC                GGATC(4/5)
AlwNI         808  CAGGGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI      1051  AGCGCT               AGC^GCT
ApaLI         872  GTGCAC               G^TGCAC
ApoI         1090  AAATTC               [GA]^AATT[CT]
AvaI          833  CCCGGG               C^[CT]CG[GA]G
BfrI          958  CTTAAG               C^TTAAG
BglII         638  AGATCT               A^GATCT
BseRI        1176  CTCCTC               GAGGAG(10/8)
BsmI          550  GAATGC               GAATGC(1/-1)
BsoBI         833  CCCGGG               C^[CT]CG[GA]G
BspMI         932  ACCTGC               ACCTGC(4/8)
BsrBI        1173  CCGCTC               CCGCTC(-3/-3)
BsrFI         300  GCCGGC               [GA]^CCGG[CT]
BstAPI       1239  GCATGTGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         305  CCAGCCTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I        300  GCCGGC               [GA]^CCGG[CT]
EarI          592  CTCTTC               CTCTTC(1/4)
Eco47III     1051  AGCGCT               AGC^GCT
EcoT14I       695  CCATGG               C^C[AT][AT]GG
HincII        256  GTCAAC               GT[CT]^[GA]AC
HindII        256  GTCAAC               GT[CT]^[GA]AC
HinfI         328  GAGTC                G^A[ACGT]TC
Ksp632I       592  CTCTTC               CTCTTC(1/4)
MaeII         275  ACGT                 A^CGT
NaeI          300  GCCGGC               GCC^GGC
NcoI          695  CCATGG               C^CATGG
NgoMIV        300  GCCGGC               G^CCGGC
NheI          573  GCTAGC               G^CTAGC
NspI         1239  GCATGT               [GA]CATG^[CT]
PflMI        1029  CCACTGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          328  GAGTC                GAGTC(4/5)
PstI          539  CTGCAG               CTGCA^G
PvuII         790  CAGCTG               CAG^CTG
SapI          591  GCTCTTC              GCTCTTC(1/4)
SmaI          833  CCCGGG               CCC^GGG
SmlI          958  CTTAAG               C^T[CT][GA]AG
StyI          695  CCATGG               C^C[AT][AT]GG
Tsp509I      1091  AATT                 ^AATT
Van91I       1029  CCACTGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI         833  CCCGGG               C^CCGGG
XmaI          833  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AfaI          120  GTAC                 GT^AC
             1041  GTAC                
ApaI          479  GGGCCC               GGGCC^C
              836  GGGCCC              
BalI          518  TGGCCA               TGG^CCA
              692  TGGCCA              
BanI          528  GGTGCC               G^G[CT][GA]CC
              745  GGCACC              
BbsI          882  GAAGAC               GAAGAC(2/6)
              916  GTCTTC              
BbvCI           8  CCTCAGC              CCTCAGC(-5/-2)
              752  CCTCAGC             
BfaI          574  CTAG                 C^TAG
             1166  CTAG                
BpmI           71  CTCCAG               CTGGAG(16/14)
              802  CTGGAG              
BpuAI         882  GAAGAC               GAAGAC(2/6)
              916  GTCTTC              
BstYI         638  AGATCT               [GA]^GATC[CT]
              737  AGATCC              
DpnII         639  GATC                 ^GATC
              738  GATC                
DsaI          483  CCGTGG               C^C[GA][CT]GG
              695  CCATGG              
EcoNI         351  CCTCTGCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              801  CCTGGAGCAGG         
HaeII         776  AGCGCC               [GA]GCGC^[CT]
             1051  AGCGCT              
HgaI          944  GCGTC                GACGC(5/10)
             1256  GCGTC               
MaeI          574  CTAG                 C^TAG
             1166  CTAG                
MboI          639  GATC                 ^GATC
              738  GATC                
MflI          638  AGATCT               [GA]^GATC[CT]
              737  AGATCC              
MluNI         518  TGGCCA               TGG^CCA
              692  TGGCCA              
MscI          518  TGGCCA               TGG^CCA
              692  TGGCCA              
MseI          959  TTAA                 T^TAA
             1106  TTAA                
MspA1I        429  CCGCTG               C[AC]G^C[GT]G
              790  CAGCTG              
NdeII         639  GATC                 ^GATC
              738  GATC                
RsaI          120  GTAC                 GT^AC
             1041  GTAC                
Sau3AI        639  GATC                 ^GATC
              738  GATC                
SfcI          373  CTACAG               C^T[GA][CT]AG
              539  CTGCAG              
Tru9I         959  TTAA                 T^TAA
             1106  TTAA                
XhoII         638  AGATCT               [GA]^GATC[CT]
              737  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   58 enzymes) #
#########################################

AccII       AciI        AluI        AspHI       AvaII       
BanII       BbvI        BcnI        BglI        BmyI        
BsaJI       BsiHKAI     BsiYI       BslI        BsmFI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
BstUI       Cac8I       CfoI        Cfr13I      DdeI        
DraII       EaeI        EcoO109I    EcoRII      FauI        
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HpaII       HphI        ItaI        MaeIII      
MboII       MnlI        MslI        MspI        MvaI        
MvnI        MwoI        NciI        NlaIII      NlaIV       
PpuMI       Sau96I      ScrFI       SfaNI       TseI        
Tsp45I      TspRI       XcmI        
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