Restriction map for pF1KB8180
 (Linear) (Six-base) MAPSORT of: KIBB8180.seq  Check: 2437  from: 1  to: 1275



KIBB8180.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:03  ..

AccI GT'mk_AC

Cuts at:      0    1007    1275 
   Size:       1007     268
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     875    1177    1275 
   Size:        875     302      98

  Fragments arranged by size: 

                 875     302      98
 
AfeI AGC'GCT

Cuts at:      0    1053    1275 
   Size:       1053     222
 
AflII C'TTAA_G

Cuts at:      0     958    1275 
   Size:        958     317
 
AleI CACnn'nnGTG

Cuts at:      0    1264    1275 
   Size:       1264      11
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     577     580     887    1275 
   Size:        577       3     307     388

  Fragments arranged by size: 

                 577     388     307       3
 
AlwNI CAG_nnn'CTG

Cuts at:      0     813    1275 
   Size:        813     462
 
ApaI G_GGCC'C

Cuts at:      0     483     840    1275 
   Size:        483     357     435

  Fragments arranged by size: 

                 483     435     357
 
ApaLI G'TGCA_C

Cuts at:      0     872    1275 
   Size:        872     403
 
ApoI r'AATT_y

Cuts at:      0    1090    1275 
   Size:       1090     185
 
AvaI C'yCGr_G

Cuts at:      0     833    1275 
   Size:        833     442
 
BanI G'GyrC_C

Cuts at:      0     528     745    1275 
   Size:        528     217     530

  Fragments arranged by size: 

                 530     528     217
 
BanII G_rGCy'C

Cuts at:      0       8      27     483     827     840    1275 
   Size:          8      19     456     344      13     435

  Fragments arranged by size: 

                 456     435     344      19      13       8
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     889     909    1275 
   Size:        889      20     366

  Fragments arranged by size: 

                 889     366      20
 
BbvCI CC'TCA_GC

Cuts at:      0       9     753    1275 
   Size:          9     744     522

  Fragments arranged by size: 

                 744     522       9
 
BglI GCCn_nnn'nGGC

Cuts at:      0     160     478     850    1133    1275 
   Size:        160     318     372     283     142

  Fragments arranged by size: 

                 372     318     283     160     142
 
BglII A'GATC_T

Cuts at:      0     638    1275 
   Size:        638     637
 
Bme1580I G_kGCm'C

Cuts at:      0     483     533     840     876    1275 
   Size:        483      50     307      36     399

  Fragments arranged by size: 

                 483     399     307      50      36
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     691    1275 
   Size:        691     584
 
BmtI G_CTAG'C

Cuts at:      0     577    1275 
   Size:        577     698
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      56     823    1275 
   Size:         56     767     452

  Fragments arranged by size: 

                 767     452      56
 
Bpu10I CC'TnA_GC

Cuts at:      0       9     569     753    1275 
   Size:          9     560     184     522

  Fragments arranged by size: 

                 560     522     184       9
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1167    1275 
   Size:       1167     108
 
BseYI C'CCAG_C

Cuts at:      0     614     792     827    1275 
   Size:        614     178      35     448

  Fragments arranged by size: 

                 614     448     178      35
 
BsiHKAI G_wGCw'C

Cuts at:      0     469     876    1250    1275 
   Size:        469     407     374      25

  Fragments arranged by size: 

                 469     407     374      25
 
BsmI GAATG_Cn'

Cuts at:      0     556    1275 
   Size:        556     719
 
Bsp1286I G_dGCh'C

Cuts at:      0       8      27     469     483     533     827     840     876 
   Size:          8      19     442      14      50     294      13      36

Cuts at:    876    1250    1275 
   Size:        374      25

  Fragments arranged by size: 

                 442     374     294      50      36      25      19      14
                  13       8
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     941    1275 
   Size:        941     334
 
BsrBI CCG'CTC

Cuts at:      0    1175    1275 
   Size:       1175     100
 
BsrFI r'CCGG_y

Cuts at:      0     300    1275 
   Size:        300     975
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1245    1275 
   Size:       1245      30
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     312    1275 
   Size:        312     963
 
BstYI r'GATC_y

Cuts at:      0     638     737    1275 
   Size:        638      99     538

  Fragments arranged by size: 

                 638     538      99
 
BtgI C'CryG_G

Cuts at:      0     483     695    1275 
   Size:        483     212     580

  Fragments arranged by size: 

                 580     483     212
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     132     402    1275 
   Size:        132     270     873

  Fragments arranged by size: 

                 873     270     132
 
BtsI GCAGTG_nn'

Cuts at:      0     404     564    1275 
   Size:        404     160     711

  Fragments arranged by size: 

                 711     404     160
 
EaeI y'GGCC_r

Cuts at:      0     206     302     342     518     692    1275 
   Size:        206      96      40     176     174     583

  Fragments arranged by size: 

                 583     206     176     174      96      40
 
EarI CTCTTCn'nnn_

Cuts at:      0     598    1275 
   Size:        598     677
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     231     970    1275 
   Size:        231     739     305

  Fragments arranged by size: 

                 739     305     231
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      56     823     875    1177    1275 
   Size:         56     767      52     302      98

  Fragments arranged by size: 

                 767     302      98      56      52
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     355     805    1275 
   Size:        355     450     470

  Fragments arranged by size: 

                 470     450     355
 
EcoO109I rG'GnC_Cy

Cuts at:      0      36     141     798     810    1141    1161    1275 
   Size:         36     105     657      12     331      20     114

  Fragments arranged by size: 

                 657     331     114     105      36      20      12
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1131    1275 
   Size:       1131     144
 
HaeII r_GCGC'y

Cuts at:      0     780    1055    1275 
   Size:        780     275     220

  Fragments arranged by size: 

                 780     275     220
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1205    1237    1275 
   Size:       1205      32      38

  Fragments arranged by size: 

                1205      38      32
 
HincII GTy'rAC

Cuts at:      0     258    1275 
   Size:        258    1017
 
MscI TGG'CCA

Cuts at:      0     520     694    1275 
   Size:        520     174     581

  Fragments arranged by size: 

                 581     520     174
 
MslI CAynn'nnrTG

Cuts at:      0     526     694    1264    1275 
   Size:        526     168     570      11

  Fragments arranged by size: 

                 570     526     168      11
 
MspA1I CmG'CkG

Cuts at:      0     431     792    1275 
   Size:        431     361     483

  Fragments arranged by size: 

                 483     431     361
 
NaeI GCC'GGC

Cuts at:      0     302    1275 
   Size:        302     973
 
NcoI C'CATG_G

Cuts at:      0     695    1275 
   Size:        695     580
 
NgoMIV G'CCGG_C

Cuts at:      0     300    1275 
   Size:        300     975
 
NheI G'CTAG_C

Cuts at:      0     573    1275 
   Size:        573     702
 
NspI r_CATG'y

Cuts at:      0    1243    1275 
   Size:       1243      32
 
PasI CC'CwG_GG

Cuts at:      0      19    1275 
   Size:         19    1256
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1035    1275 
   Size:       1035     240
 
PfoI T'CCnGG_A

Cuts at:      0    1094    1275 
   Size:       1094     181
 
PpuMI rG'GwC_Cy

Cuts at:      0      36     141     798    1141    1275 
   Size:         36     105     657     343     134

  Fragments arranged by size: 

                 657     343     134     105      36
 
PspOMI G'GGCC_C

Cuts at:      0     479     836    1275 
   Size:        479     357     439

  Fragments arranged by size: 

                 479     439     357
 
PstI C_TGCA'G

Cuts at:      0     543    1275 
   Size:        543     732
 
PvuII CAG'CTG

Cuts at:      0     792    1275 
   Size:        792     483
 
SanDI GG'GwC_CC

Cuts at:      0      36    1141    1275 
   Size:         36    1105     134

  Fragments arranged by size: 

                1105     134      36
 
SapI GCTCTTCn'nnn_

Cuts at:      0     598    1275 
   Size:        598     677
 
SfcI C'TryA_G

Cuts at:      0     373     539    1275 
   Size:        373     166     736

  Fragments arranged by size: 

                 736     373     166
 
SmaI CCC'GGG

Cuts at:      0     835    1275 
   Size:        835     440
 
SmlI C'TyrA_G

Cuts at:      0     958    1275 
   Size:        958     317
 
StyI C'CwwG_G

Cuts at:      0     695    1275 
   Size:        695     580
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     464    1275 
   Size:        464     811
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     312     756     897    1275 
   Size:        312     444     141     378

  Fragments arranged by size: 

                 444     378     312     141
 
XmaI C'CCGG_G

Cuts at:      0     833    1275 
   Size:        833     442
 

 Enzymes that do cut:

     AccI     AcuI     AfeI    AflII     AleI     AlfI    AlwNI     ApaI
    ApaLI     ApoI     AvaI     BanI    BanII     BbsI    BbvCI     BglI
    BglII Bme1580I     BmrI     BmtI     BpmI   Bpu10I    BseRI    BseYI
  BsiHKAI     BsmI Bsp1286I    BspMI    BsrBI    BsrFI   BstAPI    BstXI
    BstYI     BtgI    BtgZI     BtsI     EaeI     EarI     EciI  Eco57MI
    EcoNI EcoO109I     FalI    HaeII    Hin4I   HincII     MscI     MslI
   MspA1I     NaeI     NcoI   NgoMIV     NheI     NspI     PasI    PflMI
     PfoI    PpuMI   PspOMI     PstI    PvuII    SanDI     SapI     SfcI
     SmaI     SmlI     StyI    TaqII     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI   AflIII     AgeI     AhdI     AloI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI     BcgI
    BciVI     BclI    BfrBI     BlpI    BmgBI     BplI    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI     BsgI    BsiEI    BsiWI
    BsmBI    BspEI    BspHI    BsrDI    BsrGI   BssHII    BssSI    BstBI
   BstEII  BstZ17I   Bsu36I     ClaI    CspCI     DraI   DraIII     DrdI
     EagI  EcoICRI    EcoRI    EcoRV     FseI     FspI    FspAI  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     NarI     NdeI
     NotI     NruI     NsiI     PacI     PciI     PmeI     PmlI     PpiI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI     TatI
     TstI  Tth111I     XbaI     XhoI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com