Restriction map for pF1KB8160
##################################
# NO CUT ENZYMES (  144 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        AhdI        
Alw44I      Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvaI        AvaII       AviII       AvrII       
BalI        BamHI       BbeI        BbrPI       BcgI        
BciVI       BclI        BfrI        BglII       BlnI        
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsiEI       BsiWI       BsmBI       BsmFI       
BsoBI       BspDI       BspEI       BsrBI       BssHII      
Bst1107I    BstEII      BstPI       BstUI       BstXI       
BstZ17I     ClaI        CpoI        DraI        DraII       
DraIII      DrdI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      EcoNI       EcoO109I    
EcoO65I     EcoRV       FauI        FbaI        FseI        
FspI        HgaI        Hin1I       HincII      HindII      
HindIII     HpaI        KasI        KpnI        Ksp632I     
KspI        MaeII       MaeIII      MamI        MfeI        
MluI        MluNI       MroI        MscI        MseI        
MunI        MvnI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        RsrII       SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspI        SwaI        
TfiI        Tru9I       Tsp45I      Tth111I     Van91I      
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AfaI          750  GTAC                 GT^AC
AflIII        305  ACATGT               A^C[GA][CT]GT
BbsI          301  GAAGAC               GAAGAC(2/6)
BbvCI         708  CCTCAGC              CCTCAGC(-5/-2)
BcnI          450  CCGGG                CC^[GC]GG
BfaI          158  CTAG                 C^TAG
BglI          244  GCCAAACAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          141  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      141  GCTCAGC              GC^T[ACGT]AGC
BpuAI         301  GAAGAC               GAAGAC(2/6)
BseRI         341  CTCCTC               GAGGAG(10/8)
BsmAI         696  GAGAC                GTCTC(1/5)
Bsp1407I      749  TGTACA               T^GTACA
BspHI         297  TCATGA               T^CATGA
BspLU11I      305  ACATGT               A^CATGT
BspMI         618  GCAGGT               ACCTGC(4/8)
BsrFI         651  GCCGGT               [GA]^CCGG[CT]
BsrGI         749  TGTACA               T^GTACA
BssSI         664  CACGAG               CACGAG(-5/-1)
BstAPI        561  GCAACAGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         527  TTCGAA               TT^CGAA
BstF5I        521  GGATG                GGATG(2/0)
BstYI         722  AGATCC               [GA]^GATC[CT]
CelII         141  GCTCAGC              GC^T[ACGT]AGC
Cfr10I        651  GCCGGT               [GA]^CCGG[CT]
EaeI           56  TGGCCG               [CT]^GGCC[GA]
EcoRI         772  GAATTC               G^AATTC
EcoT22I       642  ATGCAT               ATGCA^T
FokI          521  GGATG                GGATG(9/13)
HaeII         145  AGCGCC               [GA]GCGC^[CT]
HinfI         211  GACTC                G^A[ACGT]TC
MaeI          158  CTAG                 C^TAG
MflI          722  AGATCC               [GA]^GATC[CT]
NciI          450  CCGGG                CC^[GC]GG
NsiI          642  ATGCAT               ATGCA^T
NspV          527  TTCGAA               TT^CGAA
PleI          211  GACTC                GAGTC(4/5)
PvuII          37  CAGCTG               CAG^CTG
RcaI          297  TCATGA               T^CATGA
RsaI          750  GTAC                 GT^AC
SfuI          527  TTCGAA               TT^CGAA
SspBI         749  TGTACA               T^GTACA
StuI          356  AGGCCT               AGG^CCT
XbaI          157  TCTAGA               T^CTAGA
XcmI          275  CCATACCCACATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         722  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AciI          457  GCGG                 CCGC(-3/-1)
              558  GCGG                
AlwNI         144  CAGCGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              418  CAGGAGCTG           
AocI          359  CCTCAGG              CC^T[ACGT]AGG
              516  CCTGAGG             
AspHI          43  GAGCTC               G[AT]GC[AT]^C
              139  GAGCTC              
BanI          268  GGCACC               G^G[CT][GA]CC
              648  GGTGCC              
BanII          43  GAGCTC               G[GA]GC[CT]^C
              139  GAGCTC              
BsgI          325  CTGCAC               GTGCAG(16/14)
              415  GTGCAG              
BsiHKAI        43  GAGCTC               G[AT]GC[AT]^C
              139  GAGCTC              
BsmI          289  GAATGC               GAATGC(1/-1)
              640  GAATGC              
BsrDI         462  CATTGC               GCAATG(2/0)
              474  CATTGC              
BsrI          118  ACTGG                ACTGG(1/-1)
              740  ACTGG               
BstNI         207  CCTGG                CC^[AT]GG
              347  CCAGG               
Bsu36I        359  CCTCAGG              CC^T[ACGT]AGG
              516  CCTGAGG             
Cac8I         148  GCCTGC               GC[ACGT]^[ACGT]GC
              220  GCAAGC              
CfoI           50  GCGC                 GCG^C
              146  GCGC                
Cfr13I        252  GGCCC                G^G[ACGT]CC
              513  GGCCC               
DsaI           53  CCATGG               C^C[GA][CT]GG
              198  CCATGG              
Eco81I        359  CCTCAGG              CC^T[ACGT]AGG
              516  CCTGAGG             
EcoRII        207  CCTGG                ^CC[AT]GG
              347  CCAGG               
HapII         450  CCGG                 C^CGG
              652  CCGG                
HhaI           50  GCGC                 GCG^C
              146  GCGC                
HinP1I         50  GCGC                 G^CGC
              146  GCGC                
HpaII         450  CCGG                 C^CGG
              652  CCGG                
MspI          450  CCGG                 C^CGG
              652  CCGG                
MvaI          207  CCTGG                CC^[AT]GG
              347  CCAGG               
NcoI           53  CCATGG               C^CATGG
              198  CCATGG              
NspI          305  ACATGT               [GA]CATG^[CT]
              746  GCATGT              
PstI           34  CTGCAG               CTGCA^G
              547  CTGCAG              
SacI           43  GAGCTC               GAGCT^C
              139  GAGCTC              
Sau96I        252  GGCCC                G^G[ACGT]CC
              513  GGCCC               
TaqI           84  TCGA                 T^CGA
              528  TCGA                
TthHB8I        84  TCGA                 T^CGA
              528  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   36 enzymes) #
#########################################

AcsI        AluI        AlwI        ApoI        BbvI        
BmyI        BpmI        BsaJI       BsiYI       BslI        
Bsp1286I    BssKI       DdeI        DpnII       EcoT14I     
Fnu4HI      HaeIII      HphI        ItaI        MboI        
MboII       MnlI        MslI        MspA1I      MwoI        
NdeII       NlaIII      NlaIV       Sau3AI      ScrFI       
SfaNI       SfcI        StyI        TseI        Tsp509I     
TspRI       
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