Restriction map for pF1KB8152
##################################
# NO CUT ENZYMES (  196 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AvaII       AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BcnI        BfrI        BglI        BglII       
BlnI        BmyI        BpmI        BsaHI       BsaI        
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1286I    Bsp1407I    BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssKI       Bst1107I    BstAPI      
BstBI       BstEII      BstF5I      BstNI       BstPI       
BstUI       BstXI       BstYI       BstZ17I     Bsu36I      
Cac8I       CfoI        Cfr10I      Cfr13I      CpoI        
DraI        DraII       DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRII      EcoT22I     FauI        FbaI        FokI        
FseI        FspI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HindIII     
HpaII       HphI        KasI        KpnI        KspI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MspI        MunI        MvaI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        Sau96I      SbfI        ScaI        
ScrFI       SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tsp45I      Tth111I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AfaI          105  GTAC                 GT^AC
BbsI          541  GAAGAC               GAAGAC(2/6)
BfaI          169  CTAG                 C^TAG
BlpI          436  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      436  GCTGAGC              GC^T[ACGT]AGC
BpuAI         541  GAAGAC               GAAGAC(2/6)
BsaAI         457  TACGTG               [CT]AC^GT[GA]
BsaBI         189  GATCGATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI          17  CCAAGG               C^C[ACGT][ACGT]GG
BsiYI         249  CCGAAGTGGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          249  CCGAAGTGGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI         236  GAGAC                GTCTC(1/5)
BspDI         190  ATCGAT               AT^CGAT
BsrI          369  ACTGG                ACTGG(1/-1)
BssSI         479  CACGAG               CACGAG(-5/-1)
CelII         436  GCTGAGC              GC^T[ACGT]AGC
ClaI          190  ATCGAT               AT^CGAT
DpnII         189  GATC                 ^GATC
EcoRI          27  GAATTC               G^AATTC
EcoRV         193  GATATC               GAT^ATC
EcoT14I        17  CCAAGG               C^C[AT][AT]GG
HpaI          451  GTTAAC               GTT^AAC
MaeI          169  CTAG                 C^TAG
MaeII         458  ACGT                 A^CGT
MaeIII        377  GTAAC                ^GT[ACGT]AC
MamI          189  GATCGATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI          189  GATC                 ^GATC
MslI           51  CATCATGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII         189  GATC                 ^GATC
NlaIV         263  GGTTCC               GG[ACGT]^[ACGT]CC
PleI           90  GACTC                GAGTC(4/5)
RsaI          105  GTAC                 GT^AC
Sau3AI        189  GATC                 ^GATC
SfaNI         605  GATGC                GCATC(5/9)
StyI           17  CCAAGG               C^C[AT][AT]GG


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AcsI           27  GAATTC               [GA]^AATT[CT]
              521  AAATTC              
ApoI           27  GAATTC               [GA]^AATT[CT]
              521  AAATTC              
DdeI          198  CTTAG                C^T[ACGT]AG
              437  CTGAG               
EarI          418  GAAGAG               CTCTTC(1/4)
              570  GAAGAG              
HincII        451  GTTAAC               GT[CT]^[GA]AC
              490  GTTGAC              
HindII        451  GTTAAC               GT[CT]^[GA]AC
              490  GTTGAC              
Ksp632I       418  GAAGAG               CTCTTC(1/4)
              570  GAAGAG              
MspA1I        206  CAGCTG               C[AC]G^C[GT]G
              311  CAGCTG              
MwoI           62  GCAGTGGTGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              304  GCAGCTACAGC         
NlaIII         54  CATG                 CATG^
              102  CATG                
PvuII         206  CAGCTG               CAG^CTG
              311  CAGCTG              
TaqI          191  TCGA                 T^CGA
              525  TCGA                
TfiI          295  GAATC                G^A[AT]TC
              593  GAATC               
TspRI          63  CAGTG                CA[GC]TG[ACGT][ACGT]^
              442  CAGTG               
TthHB8I       191  TCGA                 T^CGA
              525  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   12 enzymes) #
#########################################

AluI        BbvI        Fnu4HI      HinfI       ItaI        
MboII       MnlI        MseI        SfcI        Tru9I       
TseI        Tsp509I     
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