Restriction map for pF1KB8141
##################################
# NO CUT ENZYMES (  155 enzymes) #
##################################

AatII       Acc65I      AclI        AcsI        AcyI        
AflII       AflIII      AhdI        Alw44I      AlwI        
AlwNI       ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AviII       AvrII       BamHI       
BanI        BanII       BbeI        BbrPI       BbsI        
BbvCI       BclI        BfrI        BglI        BglII       
BlnI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BseRI       BsgI        BsiHKAI     BsiWI       BsmAI       
BsmBI       BsmI        Bsp1286I    Bsp1407I    BspDI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstEII      BstPI       BstXI       BstYI       BstZ17I     
CelII       ClaI        CpoI        DraI        DraII       
DraIII      DrdI        EagI        Eam1105I    EarI        
EclXI       Eco52I      EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FbaI        FseI        
FspI        HgaI        Hin1I       HindIII     HpaI        
KasI        KpnI        Ksp632I     KspI        MaeII       
MamI        MfeI        MflI        MluI        MseI        
MunI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NotI        NruI        NsiI        NspI        
PacI        PaeR7I      PflFI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SacII       
SapI        SbfI        ScaI        SfaNI       SfiI        
SfoI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        Tru9I       Tsp509I     TspRI       Tth111I     
XbaI        XcmI        XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   60 enzymes) #
###################################

AccI          171  GTCGAC               GT^[AC][GT]AC
AccII         225  CGCG                 CG^CG
AccIII        188  TCCGGA               T^CCGGA
AfaI          297  GTAC                 GT^AC
AgeI           34  ACCGGT               A^CCGGT
AocI          246  CCTGAGG              CC^T[ACGT]AGG
Aor51HI        85  AGCGCT               AGC^GCT
AvaI          130  CCCGGG               C^[CT]CG[GA]G
AvaII         134  GGACC                G^G[AT]CC
BalI           62  TGGCCA               TGG^CCA
BciVI         389  GGATAC               GTATCC(6/5)
BfaI          287  CTAG                 C^TAG
BseAI         188  TCCGGA               T^CCGGA
BsiEI         173  CGACCG               CG[GA][CT]^CG
BsmFI         133  GGGAC                GGGAC(10/14)
BsoBI         130  CCCGGG               C^[CT]CG[GA]G
BspEI         188  TCCGGA               T^CCGGA
BsrI          375  CCAGT                ACTGG(1/-1)
BstBI         313  TTCGAA               TT^CGAA
BstUI         225  CGCG                 CG^CG
Bsu36I        246  CCTGAGG              CC^T[ACGT]AGG
DpnII         222  GATC                 ^GATC
DsaI          137  CCGTGG               C^C[GA][CT]GG
EaeI           62  TGGCCA               [CT]^GGCC[GA]
Eco47III       85  AGCGCT               AGC^GCT
Eco81I        246  CCTGAGG              CC^T[ACGT]AGG
EcoNI         200  CCTTCCAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII        171  GTCGAC               GT[CT]^[GA]AC
HindII        171  GTCGAC               GT[CT]^[GA]AC
MaeI          287  CTAG                 C^TAG
MaeIII        352  GTCAC                ^GT[ACGT]AC
MboI          222  GATC                 ^GATC
MluNI          62  TGGCCA               TGG^CCA
MroI          188  TCCGGA               T^CCGGA
MscI           62  TGGCCA               TGG^CCA
MslI          354  CACCATCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I         58  CAGCTG               C[AC]G^C[GT]G
MvnI          225  CGCG                 CG^CG
NdeII         222  GATC                 ^GATC
NheI          286  GCTAGC               G^CTAGC
NlaIII        360  CATG                 CATG^
NlaIV         133  GGGACC               GG[ACGT]^[ACGT]CC
NspV          313  TTCGAA               TT^CGAA
PflMI         204  CCAGAGGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI          34  ACCGGT               A^CCGGT
PleI          350  GAGTC                GAGTC(4/5)
PstI          277  CTGCAG               CTGCA^G
PvuII          58  CAGCTG               CAG^CTG
RsaI          297  GTAC                 GT^AC
SalI          171  GTCGAC               G^TCGAC
Sau3AI        222  GATC                 ^GATC
SexAI         299  ACCTGGT              A^CC[AT]GGT
SfuI          313  TTCGAA               TT^CGAA
SgrAI          33  CACCGGTG             C[GA]^CCGG[CT]G
SmaI          130  CCCGGG               CCC^GGG
TfiI          153  GATTC                G^A[AT]TC
Tsp45I        352  GTCAC                ^GT[GC]AC
Van91I        204  CCAGAGGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI         130  CCCGGG               C^CCGGG
XmaI          130  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

BcgI          173  CGACCGAGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
               15  GCAGACTGCTCG        
BcnI          130  CCCGG                CC^[GC]GG
              131  CCGGG               
BsaJI         130  CCCGGG               C^C[ACGT][ACGT]GG
              137  CCGTGG              
BsaWI          34  ACCGGT               [AT]^CCGG[AT]
              188  TCCGGA              
BsrFI          34  ACCGGT               [GA]^CCGG[CT]
               97  ACCGGC              
BstNI          77  CCAGG                CC^[AT]GG
              300  CCTGG               
Cfr10I         34  ACCGGT               [GA]^CCGG[CT]
               97  ACCGGC              
DdeI          247  CTGAG                C^T[ACGT]AG
              344  CTAAG               
EcoRII         77  CCAGG                ^CC[AT]GG
              300  CCTGG               
FauI           50  CCCGC                CCCGC(4/6)
              101  GCGGG               
HaeII          85  AGCGCT               [GA]GCGC^[CT]
              141  GGCGCT              
HinfI         153  GATTC                G^A[ACGT]TC
              350  GAGTC               
MboII         108  GAAGA                GAAGA(8/7)
              316  GAAGA               
MvaI           77  CCAGG                CC^[AT]GG
              300  CCTGG               
NciI          130  CCCGG                CC^[GC]GG
              131  CCGGG               
SfcI          123  CTACAG               C^T[GA][CT]AG
              277  CTGCAG              


#########################################
# 3 OR MORE CUT ENZYMES (   27 enzymes) #
#########################################

AciI        AluI        BbvI        BsiYI       BslI        
BssKI       BstF5I      Cac8I       CfoI        Cfr13I      
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HpaII       HphI        ItaI        MnlI        
MspI        MwoI        Sau96I      ScrFI       TaqI        
TseI        TthHB8I     
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