Restriction map for pF1KB8128
##################################
# NO CUT ENZYMES (  150 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwI        AlwNI       AocI        Aor51HI     ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AviII       AvrII       BalI        BamHI       
BanI        BbeI        BbrPI       BbsI        BbvCI       
BcgI        BfrI        BglI        BglII       BlnI        
BlpI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BsiEI       BsiWI       BsmBI       BsmI        BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstYI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DdeI        
DraI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoRI       EcoRV       EcoT22I     FseI        FspI        
Hin1I       HincII      HindII      HindIII     HpaI        
HphI        KasI        KpnI        KspI        MaeII       
MamI        MflI        MluI        MluNI       MroI        
MscI        MspA1I      NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RcaI        RsrII       SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspI        StuI        SwaI        Tth111I     
Van91I      XbaI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccI          553  GTAGAC               GT^[AC][GT]AC
ApaI          111  GGGCCC               GGGCC^C
Asp700I       497  GAAATTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         740  GAGCTC               G[AT]GC[AT]^C
AvaI          253  CTCGAG               C^[CT]CG[GA]G
BclI          675  TGATCA               T^GATCA
BsiHKAI       740  GAGCTC               G[AT]GC[AT]^C
BsmAI          16  GTCTC                GTCTC(1/5)
BsoBI         253  CTCGAG               C^[CT]CG[GA]G
BstEII        208  GGTTACC              G^GT[ACGT]ACC
BstNI         912  CCAGG                CC^[AT]GG
BstPI         208  GGTTACC              G^GT[ACGT]ACC
BstXI         235  CCAGTTTATTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DpnII         676  GATC                 ^GATC
DraII         111  GGGCCCC              [GA]G^G[ACGT]CC[CT]
DsaI          828  CCATGG               C^C[GA][CT]GG
EaeI          845  CGGCCA               [CT]^GGCC[GA]
EarI          737  GAAGAG               CTCTTC(1/4)
EcoO109I      111  GGGCCCC              [GA]G^G[ACGT]CC[CT]
EcoO65I       208  GGTTACC              G^GT[ACGT]ACC
EcoRII        912  CCAGG                ^CC[AT]GG
FauI          109  GCGGG                CCCGC(4/6)
FbaI          675  TGATCA               T^GATCA
HaeII         319  GGCGCT               [GA]GCGC^[CT]
HgaI          106  GACGC                GACGC(5/10)
Ksp632I       737  GAAGAG               CTCTTC(1/4)
MboI          676  GATC                 ^GATC
MfeI          295  CAATTG               C^AATTG
MslI          770  CATACCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI          295  CAATTG               C^AATTG
MvaI          912  CCAGG                CC^[AT]GG
NcoI          828  CCATGG               C^CATGG
NdeII         676  GATC                 ^GATC
PaeR7I        253  CTCGAG               C^TCGAG
PleI          380  GAGTC                GAGTC(4/5)
PstI          164  CTGCAG               CTGCA^G
SacI          740  GAGCTC               GAGCT^C
SapI          737  GAAGAGC              GCTCTTC(1/4)
Sau3AI        676  GATC                 ^GATC
SfcI          164  CTGCAG               C^T[GA][CT]AG
TaqI          254  TCGA                 T^CGA
TfiI          269  GATTC                G^A[AT]TC
Tsp45I        413  GTGAC                ^GT[GC]AC
TthHB8I       254  TCGA                 T^CGA
XcmI          539  CCATTGATTTCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          253  CTCGAG               C^TCGAG
XmnI          497  GAAATTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AccII         108  CGCG                 CG^CG
              843  CGCG                
AciI          109  GCGG                 CCGC(-3/-1)
              844  GCGG                
AvaII         246  GGACC                G^G[AT]CC
              519  GGACC               
BanII         111  GGGCCC               G[GA]GC[CT]^C
              740  GAGCTC              
BbvI          896  GCAGC                GCAGC(8/12)
              819  GCTGC               
BciVI         231  GTATCC               GTATCC(6/5)
              723  GTATCC              
BcnI          213  CCGGG                CC^[GC]GG
              513  CCCGG               
BfaI          377  CTAG                 C^TAG
              562  CTAG                
BmyI          111  GGGCCC               G[AGT]GC[ACT]^C
              740  GAGCTC              
BseRI         874  GAGGAG               GAGGAG(10/8)
              916  GAGGAG              
BsmFI         245  GGGAC                GGGAC(10/14)
              644  GGGAC               
Bsp1286I      111  GGGCCC               G[AGT]GC[ACT]^C
              740  GAGCTC              
Bsp1407I      713  TGTACA               T^GTACA
              804  TGTACA              
BsrGI         713  TGTACA               T^GTACA
              804  TGTACA              
BstUI         108  CGCG                 CG^CG
              843  CGCG                
Cac8I         109  GCGGGC               GC[ACGT]^[ACGT]GC
              465  GCCTGC              
CfoI          320  GCGC                 GCG^C
              685  GCGC                
EcoT14I       828  CCATGG               C^C[AT][AT]GG
              900  CCTTGG              
HhaI          320  GCGC                 GCG^C
              685  GCGC                
HinP1I        320  GCGC                 G^CGC
              685  GCGC                
HinfI         269  GATTC                G^A[ACGT]TC
              380  GAGTC               
MaeI          377  CTAG                 C^TAG
              562  CTAG                
MseI           11  TTAA                 T^TAA
               56  TTAA                
MvnI          108  CGCG                 CG^CG
              843  CGCG                
NciI          213  CCGGG                CC^[GC]GG
              513  CCCGG               
NlaIII        549  CATG                 CATG^
              829  CATG                
SmlI          253  CTCGAG               C^T[CT][GA]AG
              880  CTTGAG              
SspBI         713  TGTACA               T^GTACA
              804  TGTACA              
StyI          828  CCATGG               C^C[AT][AT]GG
              900  CCTTGG              
Tru9I          11  TTAA                 T^TAA
               56  TTAA                
TseI          819  GCTGC                G^C[AT]GC
              896  GCAGC               


#########################################
# 3 OR MORE CUT ENZYMES (   30 enzymes) #
#########################################

AcsI        AfaI        AluI        ApoI        BsaJI       
BsgI        BsiYI       BslI        BsrI        BssKI       
BstF5I      Cfr13I      Fnu4HI      FokI        HaeIII      
HapII       HpaII       ItaI        MaeIII      MboII       
MnlI        MspI        MwoI        NlaIV       RsaI        
Sau96I      ScrFI       SfaNI       Tsp509I     TspRI       
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