Restriction map for pF1KB8128
 (Linear) (Six-base) MAPSORT of: KIBB8128.seq  Check: 4034  from: 1  to: 924



KIBB8128.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:03  ..

AccI GT'mk_AC

Cuts at:      0     554     924 
   Size:        554     370
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     393     716     924 
   Size:        393     323     208

  Fragments arranged by size: 

                 393     323     208
 
ApaI G_GGCC'C

Cuts at:      0     115     924 
   Size:        115     809
 
ApoI r'AATT_y

Cuts at:      0     349     498     615     638     924 
   Size:        349     149     117      23     286

  Fragments arranged by size: 

                 349     286     149     117      23
 
AvaI C'yCGr_G

Cuts at:      0     253     924 
   Size:        253     671
 
BanII G_rGCy'C

Cuts at:      0     115     744     924 
   Size:        115     629     180

  Fragments arranged by size: 

                 629     180     115
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     242     734     924 
   Size:        242     492     190

  Fragments arranged by size: 

                 492     242     190
 
BclI T'GATC_A

Cuts at:      0     675     924 
   Size:        675     249
 
Bme1580I G_kGCm'C

Cuts at:      0     115     924 
   Size:        115     809
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     901     924 
   Size:        901      23
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     889     924 
   Size:        889      35
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     798     806     907     924 
   Size:        798       8     101      17

  Fragments arranged by size: 

                 798     101      17       8
 
BsiHKAI G_wGCw'C

Cuts at:      0     744     924 
   Size:        744     180
 
Bsp1286I G_dGCh'C

Cuts at:      0     115     744     924 
   Size:        115     629     180

  Fragments arranged by size: 

                 629     180     115
 
BsrGI T'GTAC_A

Cuts at:      0     713     804     924 
   Size:        713      91     120

  Fragments arranged by size: 

                 713     120      91
 
BstEII G'GTnAC_C

Cuts at:      0     208     924 
   Size:        208     716
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     242     924 
   Size:        242     682
 
BtgI C'CryG_G

Cuts at:      0     828     924 
   Size:        828      96
 
EaeI y'GGCC_r

Cuts at:      0     845     924 
   Size:        845      79
 
EarI CTCTTCn'nnn_

Cuts at:      0     732     924 
   Size:        732     192
 
EcoICRI GAG'CTC

Cuts at:      0     742     924 
   Size:        742     182
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     393     716     924 
   Size:        393     323     208

  Fragments arranged by size: 

                 393     323     208
 
EcoO109I rG'GnC_Cy

Cuts at:      0     112     924 
   Size:        112     812
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     328     916     924 
   Size:        328     588       8

  Fragments arranged by size: 

                 588     328       8
 
HaeII r_GCGC'y

Cuts at:      0     323     924 
   Size:        323     601
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     177     209     924 
   Size:        177      32     715

  Fragments arranged by size: 

                 715     177      32
 
MfeI C'AATT_G

Cuts at:      0     295     924 
   Size:        295     629
 
MslI CAynn'nnrTG

Cuts at:      0     774     924 
   Size:        774     150
 
NcoI C'CATG_G

Cuts at:      0     828     924 
   Size:        828      96
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      32      64     924 
   Size:         32      32     860

  Fragments arranged by size: 

                 860      32      32
 
PsiI TTA'TAA

Cuts at:      0      29     924 
   Size:         29     895
 
PspOMI G'GGCC_C

Cuts at:      0     111     924 
   Size:        111     813
 
PstI C_TGCA'G

Cuts at:      0     168     924 
   Size:        168     756
 
SacI G_AGCT'C

Cuts at:      0     744     924 
   Size:        744     180
 
SapI GCTCTTCn'nnn_

Cuts at:      0     732     924 
   Size:        732     192
 
SfcI C'TryA_G

Cuts at:      0     164     924 
   Size:        164     760
 
SmlI C'TyrA_G

Cuts at:      0     253     880     924 
   Size:        253     627      44

  Fragments arranged by size: 

                 627     253      44
 
StyI C'CwwG_G

Cuts at:      0     828     900     924 
   Size:        828      72      24

  Fragments arranged by size: 

                 828      72      24
 
TatI w'GTAC_w

Cuts at:      0     713     804     924 
   Size:        713      91     120

  Fragments arranged by size: 

                 713     120      91
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     546     924 
   Size:        546     378
 
XhoI C'TCGA_G

Cuts at:      0     253     924 
   Size:        253     671
 
XmnI GAAnn'nnTTC

Cuts at:      0     501     924 
   Size:        501     423
 

 Enzymes that do cut:

     AccI     AcuI     ApaI     ApoI     AvaI    BanII    BciVI     BclI
 Bme1580I    BpuEI    BseRI     BsgI  BsiHKAI Bsp1286I    BsrGI   BstEII
    BstXI     BtgI     EaeI     EarI  EcoICRI  Eco57MI EcoO109I     FalI
    HaeII    Hin4I     MfeI     MslI     NcoI     PpiI     PsiI   PspOMI
     PstI     SacI     SapI     SfcI     SmlI     StyI     TatI     XcmI
     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI
     BcgI    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI
    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EciI    EcoNI    EcoRI    EcoRV     FseI
     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI     MluI
     MmeI     MscI   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI    PpuMI    PshAI    PspXI     PsrI     PvuI
    PvuII    RsrII    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TstI  Tth111I     XbaI
     XmaI     ZraI
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