Restriction map for pF1KB8126
 (Linear) (Six-base) MAPSORT of: KIBB8126.seq  Check: 632  from: 1  to: 1659



KIBB8126.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:03  ..

Acc65I G'GTAC_C

Cuts at:      0    1291    1659 
   Size:       1291     368
 
AclI AA'CG_TT

Cuts at:      0     690    1226    1659 
   Size:        690     536     433

  Fragments arranged by size: 

                 690     536     433
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     281     838    1432    1659 
   Size:        281     557     594     227

  Fragments arranged by size: 

                 594     557     281     227
 
AflII C'TTAA_G

Cuts at:      0     222    1659 
   Size:        222    1437
 
AflIII A'CryG_T

Cuts at:      0     502    1659 
   Size:        502    1157
 
AleI CACnn'nnGTG

Cuts at:      0     817    1659 
   Size:        817     842
 
AlwNI CAG_nnn'CTG

Cuts at:      0     861    1659 
   Size:        861     798
 
ApoI r'AATT_y

Cuts at:      0     545    1635    1659 
   Size:        545    1090      24

  Fragments arranged by size: 

                1090     545      24
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     179     212     841     874    1191    1224    1659 
   Size:        179      33     629      33     317      33     435

  Fragments arranged by size: 

                 629     435     317     179      33      33      33
 
BamHI G'GATC_C

Cuts at:      0    1252    1659 
   Size:       1252     407
 
BanI G'GyrC_C

Cuts at:      0     597    1291    1659 
   Size:        597     694     368

  Fragments arranged by size: 

                 694     597     368
 
BanII G_rGCy'C

Cuts at:      0    1502    1659 
   Size:       1502     157
 
BbvCI CC'TCA_GC

Cuts at:      0     259    1659 
   Size:        259    1400
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1219    1659 
   Size:       1219     440
 
BclI T'GATC_A

Cuts at:      0     923    1659 
   Size:        923     736
 
BglII A'GATC_T

Cuts at:      0    1175    1592    1659 
   Size:       1175     417      67

  Fragments arranged by size: 

                1175     417      67
 
Bme1580I G_kGCm'C

Cuts at:      0    1573    1659 
   Size:       1573      86
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     146     221     656     765    1335    1659 
   Size:        146      75     435     109     570     324

  Fragments arranged by size: 

                 570     435     324     146     109      75
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     171    1659 
   Size:        171    1488
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      48    1066    1516    1659 
   Size:         48    1018     450     143

  Fragments arranged by size: 

                1018     450     143      48
 
Bpu10I CC'TnA_GC

Cuts at:      0     259    1659 
   Size:        259    1400
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      66    1659 
   Size:         66    1593
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     374     395     404     425    1018    1048    1659 
   Size:        374      21       9      21     593      30     611

  Fragments arranged by size: 

                 611     593     374      30      21      21       9
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     445    1659 
   Size:        445    1214
 
BseYI C'CCAG_C

Cuts at:      0    1501    1659 
   Size:       1501     158
 
BsiEI CG_ry'CG

Cuts at:      0      26    1659 
   Size:         26    1633
 
BsmI GAATG_Cn'

Cuts at:      0    1530    1659 
   Size:       1530     129
 
Bsp1286I G_dGCh'C

Cuts at:      0    1502    1573    1659 
   Size:       1502      71      86

  Fragments arranged by size: 

                1502      86      71
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1272    1279    1659 
   Size:       1272       7     380

  Fragments arranged by size: 

                1272     380       7
 
BsrDI GCAATG_nn'

Cuts at:      0      89    1659 
   Size:         89    1570
 
BssSI C'ACGA_G

Cuts at:      0    1244    1659 
   Size:       1244     415
 
BstEII G'GTnAC_C

Cuts at:      0     634    1659 
   Size:        634    1025
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1302    1659 
   Size:       1302     357
 
BstYI r'GATC_y

Cuts at:      0     703     757    1175    1252    1592    1659 
   Size:        703      54     418      77     340      67

  Fragments arranged by size: 

                 703     418     340      77      67      54
 
BtgI C'CryG_G

Cuts at:      0      26     884    1433    1659 
   Size:         26     858     549     226

  Fragments arranged by size: 

                 858     549     226      26
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     995    1659 
   Size:        995     664
 
EaeI y'GGCC_r

Cuts at:      0      23    1659 
   Size:         23    1636
 
EagI C'GGCC_G

Cuts at:      0      23    1659 
   Size:         23    1636
 
EarI CTCTTCn'nnn_

Cuts at:      0     161     388     775    1659 
   Size:        161     227     387     884

  Fragments arranged by size: 

                 884     387     227     161
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     973    1201    1659 
   Size:        973     228     458

  Fragments arranged by size: 

                 973     458     228
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      48     281     838    1066    1432    1516    1659 
   Size:         48     233     557     228     366      84     143

  Fragments arranged by size: 

                 557     366     233     228     143      84      48
 
EcoRV GAT'ATC

Cuts at:      0    1483    1659 
   Size:       1483     176
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     925     957    1659 
   Size:        925      32     702

  Fragments arranged by size: 

                 925     702      32
 
KpnI G_GTAC'C

Cuts at:      0    1295    1659 
   Size:       1295     364
 
MfeI C'AATT_G

Cuts at:      0     488     602     659    1659 
   Size:        488     114      57    1000

  Fragments arranged by size: 

                1000     488     114      57
 
MluI A'CGCG_T

Cuts at:      0     502    1659 
   Size:        502    1157
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1168    1230    1659 
   Size:       1168      62     429

  Fragments arranged by size: 

                1168     429      62
 
MslI CAynn'nnrTG

Cuts at:      0     817     820    1659 
   Size:        817       3     839

  Fragments arranged by size: 

                 839     817       3
 
MspA1I CmG'CkG

Cuts at:      0    1258    1659 
   Size:       1258     401
 
NcoI C'CATG_G

Cuts at:      0     884    1433    1659 
   Size:        884     549     226

  Fragments arranged by size: 

                 884     549     226
 
NspI r_CATG'y

Cuts at:      0     919    1659 
   Size:        919     740
 
SapI GCTCTTCn'nnn_

Cuts at:      0     161    1659 
   Size:        161    1498
 
SmlI C'TyrA_G

Cuts at:      0     222    1659 
   Size:        222    1437
 
StuI AGG'CCT

Cuts at:      0    1006    1659 
   Size:       1006     653
 
StyI C'CwwG_G

Cuts at:      0     459     884    1433    1659 
   Size:        459     425     549     226

  Fragments arranged by size: 

                 549     459     425     226
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     653    1659 
   Size:        653    1006
 
TatI w'GTAC_w

Cuts at:      0     725     809    1659 
   Size:        725      84     850

  Fragments arranged by size: 

                 850     725      84
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     374     395     406     427    1659 
   Size:        374      21      11      21    1232

  Fragments arranged by size: 

                1232     374      21      21      11
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      72    1659 
   Size:         72    1587
 
XmnI GAAnn'nnTTC

Cuts at:      0     691    1528    1659 
   Size:        691     837     131

  Fragments arranged by size: 

                 837     691     131
 

 Enzymes that do cut:

   Acc65I     AclI     AcuI    AflII   AflIII     AleI    AlwNI     ApoI
     BaeI    BamHI     BanI    BanII    BbvCI    BciVI     BclI    BglII
 Bme1580I     BmrI     BplI     BpmI   Bpu10I     BsaI    BsaXI    BseRI
    BseYI    BsiEI     BsmI Bsp1286I    BspMI    BsrDI    BssSI   BstEII
    BstXI    BstYI     BtgI     DrdI     EaeI     EagI     EarI     EciI
  Eco57MI    EcoRV    Hin4I     KpnI     MfeI     MluI     MmeI     MslI
   MspA1I     NcoI     NspI     SapI     SmlI     StuI     StyI    TaqII
     TatI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AfeI     AgeI     AhdI     AlfI     AloI
     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BbeI
     BbsI     BcgI    BfrBI     BglI     BlpI    BmgBI     BmtI    BpuEI
    BsaAI    BsaBI    BsaHI    BsaWI     BsgI  BsiHKAI    BsiWI    BsmBI
    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII   BstAPI    BstBI
  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
  EcoICRI    EcoNI EcoO109I    EcoRI     FalI     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     MscI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI  Tth111I     XbaI     XhoI     XmaI     ZraI
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