Restriction map for pF1KB8111
##################################
# NO CUT ENZYMES (  114 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwNI       Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AviII       AvrII       BbvCI       BcgI        BclI        
BfaI        BfrI        BglII       BlnI        BlpI        
Bpu1102I    BsaBI       BseAI       BsiEI       BsiHKAI     
BsiWI       BsmBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrDI       BsrGI       BssHII      
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstXI       BstZ17I     CelII       ClaI        CpoI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoNI       EcoO65I     EcoRI       
EcoRV       FbaI        FseI        FspI        HpaI        
KpnI        KspI        MaeI        MamI        MfeI        
MluI        MroI        MunI        NdeI        NheI        
NotI        NruI        NspV        PacI        PinAI       
PmeI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        SexAI       SfiI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        Sse8387I    
SspBI       SspI        StuI        SwaI        TfiI        
XbaI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AccII         404  CGCG                 CG^CG
AspI           96  GACTGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          296  CTCGAG               C^[CT]CG[GA]G
BalI            2  TGGCCA               TGG^CCA
BamHI        1461  GGATCC               G^GATCC
BbeI          181  GGCGCC               GGCGC^C
BbrPI        1035  CACGTG               CAC^GTG
BciVI         994  GGATAC               GTATCC(6/5)
BglI         1142  GCCAGTTTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI        1035  CACGTG               [CT]AC^GT[GA]
BsaWI         621  ACCGGA               [AT]^CCGG[AT]
BsmI          119  GCATTC               GAATGC(1/-1)
BsoBI         296  CTCGAG               C^[CT]CG[GA]G
BsrBI         388  GAGCGG               CCGCTC(-3/-3)
BssSI         934  CTCGTG               CACGAG(-5/-1)
BstUI         404  CGCG                 CG^CG
DraI         1531  TTTAAA               TTT^AAA
EaeI            2  TGGCCA               [CT]^GGCC[GA]
EarI         1302  GAAGAG               CTCTTC(1/4)
EcoT22I       807  ATGCAT               ATGCA^T
HaeII         181  GGCGCC               [GA]GCGC^[CT]
HincII       1468  GTCAAC               GT[CT]^[GA]AC
HindII       1468  GTCAAC               GT[CT]^[GA]AC
HindIII       430  AAGCTT               A^AGCTT
KasI          181  GGCGCC               G^GCGCC
Ksp632I      1302  GAAGAG               CTCTTC(1/4)
MluNI           2  TGGCCA               TGG^CCA
MscI            2  TGGCCA               TGG^CCA
MvnI          404  CGCG                 CG^CG
NarI          181  GGCGCC               GG^CGCC
NcoI          149  CCATGG               C^CATGG
NsiI          807  ATGCAT               ATGCA^T
PaeR7I        296  CTCGAG               C^TCGAG
PflFI          96  GACTGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         819  CCAGCTTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        1035  CACGTG               CAC^GTG
PmlI         1035  CACGTG               CAC^GTG
ScaI          581  AGTACT               AGT^ACT
SfoI          181  GGCGCC               GGC^GCC
SmlI          296  CTCGAG               C^T[CT][GA]AG
Tth111I        96  GACTGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        819  CCAGCTTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI          296  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AocI          235  CCTGAGG              CC^T[ACGT]AGG
              919  CCTAAGG             
ApaI          141  GGGCCC               GGGCC^C
             1380  GGGCCC              
BanI          181  GGCGCC               G^G[CT][GA]CC
             1343  GGCACC              
BbsI         1099  GAAGAC               GAAGAC(2/6)
             1164  GTCTTC              
BcnI          178  CCCGG                CC^[GC]GG
             1650  CCCGG               
BpuAI        1099  GAAGAC               GAAGAC(2/6)
             1164  GTCTTC              
BsaI          535  GAGACC               GGTCTC(1/5)
              929  GAGACC              
BseRI        1173  CTCCTC               GAGGAG(10/8)
             1176  CTCCTC              
BsmAI         535  GAGAC                GTCTC(1/5)
              929  GAGAC               
BsmFI         611  GGGAC                GGGAC(10/14)
              370  GTCCC               
BsrFI         184  GCCGGC               [GA]^CCGG[CT]
             1086  GCCGGC              
BstYI        1461  GGATCC               [GA]^GATC[CT]
             1613  AGATCC              
Bsu36I        235  CCTGAGG              CC^T[ACGT]AGG
              919  CCTAAGG             
Cfr10I        184  GCCGGC               [GA]^CCGG[CT]
             1086  GCCGGC              
DsaI          149  CCATGG               C^C[GA][CT]GG
              281  CCACGG              
Eco81I        235  CCTGAGG              CC^T[ACGT]AGG
              919  CCTAAGG             
MflI         1461  GGATCC               [GA]^GATC[CT]
             1613  AGATCC              
MslI         1155  CATGCAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1595  CACACAAGTG          
NaeI          184  GCCGGC               GCC^GGC
             1086  GCCGGC              
NciI          178  CCCGG                CC^[GC]GG
             1650  CCCGG               
NgoMIV        184  GCCGGC               G^CCGGC
             1086  GCCGGC              
NspI          198  GCATGC               [GA]CATG^[CT]
             1154  GCATGC              
PstI          161  CTGCAG               CTGCA^G
              193  CTGCAG              
PvuII          19  CAGCTG               CAG^CTG
             1423  CAGCTG              
SfcI          161  CTGCAG               C^T[GA][CT]AG
              193  CTGCAG              
SphI          198  GCATGC               GCATG^C
             1154  GCATGC              
Tsp45I       1033  GTCAC                ^GT[GC]AC
             1500  GTCAC               
TspRI         159  CACTG                CA[GC]TG[ACGT][ACGT]^
             1385  CAGTG               
XcmI          281  CCACGGTTGTCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              716  CCAACTCATTCGTGG     
XhoII        1461  GGATCC               [GA]^GATC[CT]
             1613  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   71 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AluI        
AlwI        ApoI        AvaII       BanII       BbvI        
BmyI        BpmI        BsaHI       BsaJI       BsgI        
BsiYI       BslI        Bsp1286I    BspMI       BsrI        
BssKI       BstF5I      BstNI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
MaeII       MaeIII      MboI        MboII       MnlI        
MseI        MspA1I      MspI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       PleI        PpuMI       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
StyI        TaqI        Tru9I       TseI        Tsp509I     
TthHB8I     
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